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Dihydromyricetin promotes autophagy and apoptosis in head and neck squamous cell carcinoma cells through STAT3/Bcl-2 signaling pathway

Man-Ying WANG ; Xiao-Jun DING ; Yang SUN ; You-Cheng YU

Chinese Journal of Clinical Medicine.2017;24(4):571-576. doi:10.12025/j.issn.1008-6358.2017.20160961

Objective:To study the effects of dihydromyricetin(DHM)on cell proliferation and apoptosis of head and neck squamous cell carcinoma(HNSCC).Methods:SCC-25 cancer cells were treated with 12.5,25,50 μmol/L DHM for 6,12,and 24 h.SCC-25 cell apoptosis was detected by flow cytometry.Immunofluorescence was used to observe the emergence of autophagosomes in HNSCC cells.Western blotting was used to detect the expression of apoptosis and autophagy markers in SCC-25.Objective:After treatment of 12.5,25,and 50 μmol/L DHM for 24 h,the apoptosis of SCC-25 cells gradually increased;the apoptotic markers CL-PARP,Bax,and CL-casp3 gradually increased and Bcl-2 gradually decreased(P<0.05).After treatment of 12.5,25,and 50 μmol/L DHM for 24 h,in line with the apoptosis,the autophagy markers of SCC-25 cells gradually changed,the expression of Beclin 1,p62 and the ratio of LC3Ⅱ/LC3Ⅰunderwent changes which were signs of autophagy and the p-STAT3 expression was up-regulated;the ratio of LC3 Ⅱ/LC3 Ⅰ gradually increased,Beclin 1 increased,p62 gradually decreased(P<0.05).Inhibition of autophagy could promote apoptosis of SCC-25 cells which were induced by DHM,increase apoptosis markers CL-PARP and Bax,and decrease Bcl-2(P<0.05).Conclusions:DHM can induce apoptosis and increase autophagy of HNSCC cells.The mechanism is related to STAT3 signaling pathway.Inhibition of autophagy can promote apoptosis of HNSCC cells induced by DHM.

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Effect of cigarette smoke extract on differentiation of bronchial epithelial cells mediated by MAPK/FOXA2

Chun-Ling DU ; Yan-Hong DUAN ; Lei ZHOU ; Bo WU

Chinese Journal of Clinical Medicine.2017;24(4):577-581. doi:10.12025/j.issn.1008-6358.2017.20160777

Objective:To explore the effect of cigarette smoke extract(CSE)on the differentiation of bronchial epithelial cells and the role of mitogen-activated protein kinase(MAPK)/forkhead box A2(FOXA2)signaling pathways in this process.Methods:BEAS-2B cells were cultivated and treated respectively with cigarette smoke extract(CSE),CSE and ERK inhibitor U0126,CSE and JNK inhibitor SP600125,CSE and p38 inhibitor SB203580.Thus the blank control group and cigarette smoke extract(CSE)treatment group,CSE+ERK inhibitor U0126 treatment group,CSE+JNK inhibitor SP600125 treatment group,and CSE+p38 inhibitor SB203580 treatment group were set.ELISA was developed for detecting the protein levels of phosphorylated ERK1/2,JNK,and p38.The mRNA and protein levels of FOXA2,E-cadherin,CD44 and ZO-1 were measured by real-time fluorescent quantitative PCR and Western blotting,respectively.Objective:Compared with the blank control group,the phosphorylated ERK1/2,JNK,and p38 protein levels were significantly increased(P<0.05),and the mRNA and protein expression of FOXA2,E-cadherin,CD44 and ZO-1 were significantly decreased(P<0.05)when cells were treated with CSE.CSE treatment with ERK,JNK or p38 inhibitors significantly improved the expression of the above genes and proteins(P<0.05).Conclusions:Smoking can affect the differentiation of bronchial epithelial cells,and the MAPK/FOXA2 signaling pathway plays an important regulatory role in this process.

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Clinical and pathological analysis of collagen type Ⅲ glomerualopathy

Shao-Min GONG ; Wei-Li LUO ; Shi JIN ; Xue-Guang LIU ; Xiao-Qiang DING ; Hong LIU

Chinese Journal of Clinical Medicine.2017;24(4):582-586. doi:10.12025/j.issn.1008-6358.2017.20170140

Objective:To provide basis for the diagnosis and treatment of collagen type Ⅲ glomerualopathy by analyzing the clinical and pathological data of patients.Methods:After searching the data of the patients receiving renal biopsy from March 2007 to June 2015 in nephrology department,the clinical and pathological features of patients who were proven as collagen type Ⅲ glomerualopathy were analyzed.Objective:Two male and two female patients aged(45.5±10.7)years old were included.None of the patients were reported to have positive familial history of any kidney diseases.None of them were found to have extra renal involvement.The most common clinical manifestations were proteinuria,hypertension and impaired renal function.Membrane proliferative glomerulonephritis,nodular glomerulosclerosis,focal segmental proliferative and sclerosis glomerulonephritis were found under the light microscopy.One of the patients was accompanied by IgA nephropathy.As for the follow-up results,1 case with minor declined eGFR and proteinuria less than 0.5 g/24 h remained stable in kidney function and proteinuria,2 cases responding to glucocorticoids and immunosuppressive agents had improvement in renal function and partial remission of proteinuria,and 1 case developed into end-stage renal disease and received hemodialysis.Conclusions:The sporadic cases of collagen type Ⅲ glomerualopathy may be underestimated in clinical practice,characterized by proteinuria,hypertension,and renal dysfunction.The light microscope pathological features of renal biopsy are various,and they are easy to be missed.Immunohistochemistry and electronic microscopy are essential to confirm the diagnosis.

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Expression of PFKFB3 in human colon cancer and its clinical significance

Mi-Mi ZHANG ; Yue-Jun HE

Chinese Journal of Clinical Medicine.2017;24(4):587-590. doi:10.12025/j.issn.1008-6358.2017.20160848

Objective:To investigate the expression of PFKFB3 in human colon cancer and its clinical significance.Methods:Reverse transcriptase polymerase chain reaction(RT-PCR)and immunohistochemistry were used to detect the mRNA and protein expression levels of PFKFB3 in cancer and adjacent tissues of 156 human colon cancer patients.The relationship of PFKFB3 expression with clinicopathological features and prognosis of colon cancer patients was analyzed by statistical analyses.Objective:PFKFB3 mRNA and protein levels were significantly increased in tumor tissues compared to adjacent non-malignant tissues.Furthermore,correlation analysis indicated that PFKFB3 mRNA high expression in colon cancer tissues was significantly associated with intravascular cancer embolus,lymph node metastasis,and TNM staging(P<0.05).Survival analysis showed a poor prognosis of patients with high expression of PFKFB3 mRNA level(P<0.05).Conclusions:PFKFB3 is highly expressed in colon cancer.The expression level of PFKFB3 is correlated with the malignant phenotype and poor prognosis of colon cancer.

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Clinical analysis of primary malignant tumors of the trachea and main bronchus

Li CHEN ; Cheng ZHAN ; Yu-Lin JIN ; Xiao-Dong YANG ; Meng-Kun SHI ; Yong-Xing ZHANG ; Qun WANG

Chinese Journal of Clinical Medicine.2017;24(4):591-594. doi:10.12025/j.issn.1008-6358.2017.20170051

Objective:To investigate the clinical features,diagnosis and surgical treatment of primary malignant tumors of the trachea and main bronchus.Methods:Clinical data of 35 cases with primary malignant tumors of the trachea and main bronchus in Zhongshan Hospital,Fudan University between January 2005 and December 2015 were analyzed retrospectively.Objective:The clinical symptoms were cough,sputum,bloody sputum,hemoptysis,shortness of breath,chest tightness,thoracalgia,and fever.There were 13 cases(37.1％)proved to be misdiagnosed in the early stage.All patients underwent surgical treatment without complications,and 3 patients were received postoperative radiotherapy.Among the 31 cases received postoperative follow-up,26 cases survived,5 cases died.The average disease-free interval was(34±16.2)months(16 to 49 months)and the median survival time was 50 months(16-138 months).Conclusions:Primary malignant tumors of the trachea and main bronchus without special clinical manifestations are easy to be misdiagnosed so that early bronchoscopy and chest CT examination are necessary.Active surgery treatment for primary malignant tumors of the trachea and main bronchus can achieve generate good prognosis.

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Clinical efficacy of biportal video-assisted thoracoscopic lobectomy in non-small cell lung cancer

Lei-Lei PENG ; Yi ZHANG ; Liang XUE ; Hong FAN

Chinese Journal of Clinical Medicine.2017;24(4):595-597. doi:10.12025/j.issn.1008-6358.2017.20170108

Objective:To explore the clinical efficacy of biportal video-assisted thoracoscopic surgery(VATS)in the treatment of non-small cell lung cancer.Methods:A total of 536 patients with non-small cell lung cancer receiving surgical resection from January 2013 to December 2016 were enrolled.The surgery was performed as video-assisted thoracoscopic surgery,by which 3-portal VATS for 167 patients(3-portal group)and biportal VATS for remaining 369 patients(biportal group).The clinical effect in two groups was compared.Objective:The statistical significances of following parameters were not found in two groups including operation time,intraoperative blood lose,lymph node dissection number,the intraoperative conversion rate,drainage on 1st day after surgery,duration of chest drainage,duration of hospital stay and incidence of postoperative complications.However,compared with 3-portal group,visual analogue scores(VAS)on 1st day,5th day and 30th day in biportal group were significantly lower.Conclusions:There is no significant difference between biportal VATS and 3-portal VATS in safety and effectiveness.Biportal VATS is a better recommended skill because of less postoperative discomforts.

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Comparison of clinical efficacy of uterine fibroids by different surgical proceduresabdominal open surgery, conventional laparoscopy and robot-assisted laparoscopy

Sheng-Lian LU ; Xian-Wei CHEN ; Sheng-Yun CAI

Chinese Journal of Clinical Medicine.2017;24(4):598-600. doi:10.12025/j.issn.1008-6358.2017.20160920

Objective:To study the clinical effect and application value of abdominal open surgery,conventional laparoscopy,robot-assisted laparoscopy for the treatment of uterine fibroids.Methods:The clinical data of 54 patients with uterine fibroids admitted to Changhai Hospital of Navy Medical University from December 2012 to October 2013 were analyzed retrospectively.They were divided into the open surgery group,the conventional laparoscopy group and the robot-assisted laparoscopy group.The operation status and postoperative complications were compared between the three groups.Objective:The surgical time of the robot-assisted laparoscopy group had no significant difference with the other two groups,but the amount of bleeding was significantly less than that in the other two groups(P<0.05),and the intestinal function recovery time was greatly shortened(P<0.05).There were no significant differences between the three groups in postoperative 24 h drainage and postoperative complication rate.The length of postoperative hospital stay of the robot-assisted laparoscopy group and the conventional laparoscopy group was significantly less than that in the open surgery group(P<0.05).There was 1 case of incision pain after operation in the open surgery group.Conclusions:The effect of robot-assisted laparoscopy for the treatment of uterine fibroids is accurate,with less intraoperative bleeding and fast postoperative intestinal function recovery.It is conducive to early recovery,and of high clinical application value.

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Application of biliary drainage with single pigtail plastic pancreatic stent after ERCP treating choledocholithiasis

Jing-Ze LI ; Wen-Jia ZHANG ; Ming XU ; Qing-He TANG

Chinese Journal of Clinical Medicine.2017;24(4):601-604. doi:10.12025/j.issn.1008-6358.2017.20170184

Objective:To discuss the clinical efficacy of biliary drainage with single pigtail plastic pancreatic stent after endoscopic retrograde cholangiopancreatography(ERCP)treating choledocholithiasis.Methods:Clinical data of 105 patients with common bile duct stones treated by ERCP from October 2014 to October 2016 in Shanghai East Hospital were analyzed retrospeoctively.These patients were divided into stenting group(n=38)and control group(n=67).The stenting group received ERCP+endoscopic sphincterotomy(EST)/endoscopic papillary balloon dilatation(EPBD)+calculus removed+single pigtail plastic pancreatic stent for biliary drainage,while the control group underwent ERCP+EST/EPBD+calculus removed+ENBD.The incidences of post-ERCP acute pancreatitis(PEP)in the two groups were compared.The stent fall and dislodging in stenting group were observed.Objective:The incidence of PEP was 13.1％(5/38)in stenting group and 14.9％(10/67)in control group.In the stenting group,3 patients had stent dislodged on the first day after ERCP.One patient got stent removed from bile duct on the third day due to upper abdomen discomfort.The stents in 8 patients were removed successfully by endoscopy while they failed in self-releasing in 2 weeks.In the other 26 patients,without complications,the stents dislodged successfully and were excreted outside through the digestive tract.Conclusions:After ERCP treating choledocholithiasis,the application of single pigtail plastic pancreatic stent bile duct drainage can prevent the incidence of PEP,reduce the severity degree of PEP,and the stent can successfully dislodged without complications.

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Laparoscopic versus open tension-free surgery for adult unilateral primary inguinal hernia:a Meta-analysis

Jiang LI ; Ming-Ming NIE ; Tian-Hang LUO ; Jun-Chi YANG ; Zheng SHI ; Jian-Wei BI

Chinese Journal of Clinical Medicine.2017;24(4):605-610. doi:10.12025/j.issn.1008-6358.2017.20161127

Objective:To evaluate and compare the efficacy of laparoscopic and open tension-free surgery for adult unilateral primary inguinal hernia.Methods:Randomized controlled trial(RCT)reports published from 2006 to 2016 were searched in the PubMed,EMBASE,Web of Science,CBM and VIP databases.Data related to the clinical outcome,which compared the efficacy of laparoscopic and open surgery for adult unilateral primary inguinal hernia,were independently extracted and analyzed by two reviewers according to Cochrane Handbook for Systematic Reviews of Interventions 5.0.1.Statistical analysis was performed using RevMan 5.3 software.Objective:A total of 3 200 adult patients with unilateral primary inguinal hernia from 12 RCTs in 13 papers were enrolled in this Meta-analysis.Compared with open surgery,the cost of hospitalization in laparoscopic repair group increased(SMD=4.99,95％CI 3.13-6.85,P<0.001),and the rates of complication occurrence(RR=0.82,95％CI 0.68-0.98,P=0.03),NRS(MD=-1.67,95％CI-2.29——1.05,P<0.001)and persistent pain recurrence(RR=0.40,95％CI 0.23-0.69,P=0.001)reduced.However,there was no significant difference in the operation time(MD=0.14,95％CI 2.69-2.97,P=0.92)and the recurrence rate(RR=0.96,95％CI 0.32-2.88,P=0.06).Conclusions:Compared with open surgery,laparoscopic surgery is superior for adult primary unilateral inguinal hernia,but with higher cost.Accordingly,the appropriate surgical operation should be selected.

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Awareness of diabetes and its influencing factors among the elderly population in Shanghai rural community

Chao-Yu ZHU ; Yue-Qin MAO ; Chun-Jun MA ; Zhi-Jun ZHU ; Mu-Song LIU ; En-Fang FAN ; Li WEI

Chinese Journal of Clinical Medicine.2017;24(4):611-614. doi:10.12025/j.issn.1008-6358.2017.20161197

Objective:To investigate the awareness of diabetes and to explore the influencing factors of it among the elderly population in Shanghai rural community.Methods:A cross-sectional study method was used in the research.Epidemiological investigation was undertaken in residents of 40-75 years old in Yunsong Commuinity of Pudong New Area in March to April,2014.Among the population,diabetic patients were selected to conducted questionnaire survey,physical examination,and laboratory testing.Objective:A total of 256 diabetic patients completed questionnaires.The awareness rate of diabetes was 50.78％(130/256).The awareness rate of diabetes indicated that the statistical significance was found in patients with different educational background,blood lipid level,fasting blood-glucose level,HbA1c and exercise habits(P<0.05).The multi-factor logistic stepwise regression analysis showed that the educational level,glycosylated hemoglobin level and exercise habits were the main independent influencing factors for the awareness rate of diabetes.Conclusions:The level of awareness rate of diabetes in Shanghai Yunsong community is in the moderate level.Education should be strengthened to increase the rate of diabetes awareness for patients with low educational background,worse glucose control and no exercise habit.

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