Chinese Journal of Clinical Medicine 2014;(3):239-241

Effect of Interferon αon the Expression and Activity of Transcription Factor Sp 1 in Hepatocarcinoma Cells

Qi PAN 1 ; Wanyong CHEN ; Kai ZHU ; Jubo ZHANG ; Yiming ZHAO ; Xiaodong ZHU ; Huichuan SUN ; Lu NG WA ; Ning REN

Affiliations

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Keywords

Hepatocarcinoma; T ranscription factor; Sp1; Interferon-α

Country

China

Language

Chinese

Abstract

Objective:To evaluate the effect of interferon-αon the expression and activity of transcription factor specificity pro-tein 1(Sp1) in hepatocarcinoma cells .Methods :The effect of interferon-αon the expression and phosphorylation level of Sp1 in hepatocarcinoma cell line M HCC97H were detected by Western blotting .The effect of interferon-α on the activity of Sp1 was analyzed by electrophoretic mobility shift assay (EMSA) .Results :Interferon-α significantly reduced the expression and phos-phorylation level of Sp1 ,and the reducement on phosphorylation was more distinct .Interferon-α also inhibited the activity of Sp1 significantly .Conclusions :Interferon-αinhibits the activity of Sp1 by down regulating the expression and phosphorylation level of Sp1 .Sp1 may be an optional predictor of clinical efficacy of interferon-αon hepatocarcinoma .