Chinese Journal of Clinical Medicine 2014;(3):259-261

The Expression of MicroRNAs in HT-29 Cells with Lipopolysaccharide-Induced Inflammation

Haibo WU 1 ; Zhigang CAI ; Shaoming NG ZHA ; Heng ZHANG ; Lei WANG ; Xiaoping XU

Affiliations

+expand

Keywords

MicroRNA; HT-29; Inflammation; Lipopolysaccharide

Country

China

Language

Chinese

Abstract

Objective:To study the expression of microRNAs (miRNAs) in colon adenocarcinoma cell line HT-29 with lipopo-lysaccharide-induced(LPS-induced) inflammation .Methods :HT-29 cells were treated with 400 ng/mL LPS for 48 h to establish inflammatory HT-29 cell model (LPS group) ,and HT-29 cells without LPS treatment were viewed as the control group .Total RNA from the two groups was isolated with Trizol reagent ,and IL-6 and CRP were examined .The expression of miRNAs was determined by Real-time PCR .Results:Compared with the control group ,the mRNA levels of IL-6 and CRP were up-regulated in LPS group .The expression levels of miR-22 ,miR-26 ,miR-214 in LPS group were lower than those in the control group , while miR-23 level was higher than that in the control group .The levels of miR-24 ,miR-30 and miR-181 were not statistically different between the two groups .Conclusions :MiRNAs may play important roles in LPS-induced inflammation of adenocarci-noma cells .