Journal Detail Information

1

Cite

Share

Fine Mapping of a Deafness Mutation hml on Mouse Chromosome 10.

Qing Yin ZHENG ; Belinda S HARRIS ; Patricia F WARD-BAILEY ; Heping YU ; Roderick T BRONSON ; Muriel T DAVISSON ; Kenneth R JOHNSON

Academic Journal of Xi'an Jiaotong University.2004;25(3):209-212.

OBJECTIVE: to map a mouse deafness gene, identify the underlying mutation and develop a mouse model for human deafness. METHODS: genetic linkage cross and genome scan were used to map a novel mutation named hypoplasia of the membranous labyrinth (hml), which causes hearing loss in mutant mice. RESULTS: 1. hml was mapped on mouse Chr 10 (~43 cM from the centromere) suggests that the homologous human gene is on 12q22-q24, which was defined on the basis of known mouse-human homologies (OMIM, 2004). 2. This study has generated 25 polymorphic microsatellite markers, placed 3 known human genes in the correct order in a high-resolution mouse map and narrowed the hml candidate gene region to a 500kb area.

2

Cite

Share

Alteration of HB-EGF in psoriatic epidermis with the treatment of retinoid acid

Yan ZHENG

Academic Journal of Xi'an Jiaotong University.2003;15(1):41-43.

Objective. To investigate the mechanism of tazarotene in active psoriasis vulgaris. Methods. HB-EGF protein in active psoriatic lesions before and 10 days after the treatment of tazarotene was detected by immunohistochemistry. Results. There was nearly no expression of HB-EGF in psoriatic lesions (9.1%), but after the treatment by tazarotene, there was expression of HB-EGF not only in basal layer (95.5%), but also focal expression in suprabasal layers of epidermis (77.3%). Conclusion. Tazarotene inhibit proliferation and induce apoptosis of keratinocytes through upregulating expression of HB-EGF in psoriatic epidermis.

3

Cite

Share

One probable mechanism of the learning-memory damage by lead: The changes of NOS in hippocampus

Jing WANG

Academic Journal of Xi'an Jiaotong University.2003;15(1):47-50.

Objective: To study the effects of lend on the activity and expression of nitric oxide synthase (NOS) and relationship between the effects of lead on learning-memory and changes of NOS in subfields of hippocampus. Methods. Y-maze test was used to study the effects of lead on ability of learning-memory; NADPH-d histochemistry and immunohistochemistry methods were used to investigate the changes of NOS in subfields of hippocampus. Results. Compared with the control group, the ability of learning-memory in lead-exposed rats was significantly decreased (P< 0.05); the number of NOS positive neurons in CA1 region and dentate gyrus of lead-exposed rats was significantly decreased(P<0.05), but no marked changes in CA3 region; the number of nNOS positive neurons in CA1 of lead-exposed rats was also significantly decreased (P<0.05), but no obvious changes in CA3. Conclusion. Lead could damage the ability of learning-memory in rats. Lead could decrease the activity and expression of NOS in hippocampus and had different effects on NOS in different subfields of hippocampus. The changes of NOS in hippocampus induced by lead may be the mechanism of the learning-memory damage by lead.

4

Cite

Share

Cellular immunity effect of leukemia vaccine on tumor burden rat

Wanhong ZHAO

Academic Journal of Xi'an Jiaotong University.2003;15(1):51-54.

Objective: To evaluate the effect of the active specific immunotherapy with leukemia vaccine in the malignant hematopoietic diseases. Methods: We established the animal models by inoculating C57BL/6 rats with FBL-3 erythroleukemia cells and prepared three types of tumor vaccine, which were administered on the rats respectively. The MTT colorimetric assay was adopted 2 and 4 weeks later to test the cytotoxicity of macrophage (MΦ) and that of cytotoxic T lymphocyte(CTL) derived from the rats injected with tumor vaccines, and compared the results with the control group. Results: With the growth of erythroleulemia cells in the rats, the cellular immunity was seriously depressed, and the inhibition of specific cellular immunity was later than that of non-specific cellular immunity. The tumor vaccine made from inactivated tumor cells, IFA and cytokines (rGM-CSF, rIL-2 and rIL-6), promote the cellular immunity of tumor burden rats, especially the specific cellular immunity more efficiently than that of tumor vaccine made from inactivated tumor cells and IFA, but the third vaccine made from inactivated tumor cells alone has no effect. Conclusion: The tumor vaccine made from inactivated tumor cells with addition of IFA and cytokines (rGM-CSF, rIL-2 and rIL-6) provides a promising future in the active specific immunotherapy against hematopoietic tumor.

5

Cite

Share

Study on differentiation of rats embryonic stem cells cultured in BRL-CM into neural precursor cells

Xiaozhi ZHANG

Academic Journal of Xi'an Jiaotong University.2003;15(1):55-58.

Objective: To investigate whether buffalo rat liver cell-conditioned medium (BRL-CM) can be used as the culture medium of embryonic stem (ES) cells, and to get relatively pure neural precursor cells (NPCs) for treatment aim. Methods: Mouse ES cells were cultured in BRL-CM and medium contain leukemia inhibitory factor (LIF), respectively. NPCs were selectively cultured in serum-free medium. Alkaline phosphatase activity was visualized with NBT/BCIP and nestin antigen was detected with immunocytochemical methods. Results: BRL-CM could be used as an efficiency culture condition instead of LIF in ES cells culture. About 86% of cells derived from ES cells in the serum-free culture were NPCs. Conclusion: BRL-CM can replace LIF to use in ES cell culture. High purity of NPC can be induced from ES cells with serum-free culture method.

6

Cite

Share

The application of cortical somatosensory evoked potential monitoring in child scoliosis surgery

Bin BAI

Academic Journal of Xi'an Jiaotong University.2003;15(1):59-61.

Objective: To study the application value of cortical somatosensory evoked potential (CSEP) monitoring in child scoliosis surgery. Methods: In surgeries of fifty-one children with scoliosis, the CSEP changes were continuously recorded by evoked potential instrument. The operations were performed under the guidance of CSEP monitoring. Results: Before propping and reshaping, the latencies and amplitudes in all cases had no change. During propping and reshaping, the latencies of all cases were slowly elongated, but all less than 10 percent. The amplitudes in 15 cases dropped to 55 percent, but returned to 80 percent 3-8 minutes after stopping the operations or partially loosening the propped rods at once. The amplitude in one case suddenly dropped to 37 percent and returned to 54 percent half an hour after loosening the propped rods at once and recovered to the normal range one day after operation. All cases got ideal orthopedic results and no one had neurological complications post operation. Conclusion: CSEP can accurately monitor the spinal injury and has a great value in preventing the spinal injury in child scoliosis surgery.

7

Cite

Share

Cloning and sequencing of matured fragment of human nerve growth factor gene

Wei MA

Academic Journal of Xi'an Jiaotong University.2003;15(1):62-65.

Objective: Molecular cloning and sequencing of the human matured fragment of human nerve growth factor(NGF) gene. Methods: Extracting the human genomic DNA from the white blood cells as templates, the gene of NGF was cloned by using PCR and T-vector cloning method. Screening the positive clones and identified by the restriction enzymes, and then the cloned amplified fragment was sequenced and analyzed. Results: DNA sequence comparison the cloned gene of NGF with the GenBank (V01511) sequence demonstrated that both of sequences were identical, 354bp length. Conclusion: Cloning the NGF gene from the human genomic DNA has paved the way for further study on gene therapy of nerve system injury.

8

Cite

Share

Study of eck gene exon-3 from human normal tissue and breast cancer cell line

Yaochen LI

Academic Journal of Xi'an Jiaotong University.2003;15(1):66-70.

Objective: To establish a method cloning the exon 3 of eck gene from normal tissue and ZR-75-1 cell line (a human breast cancer cell line) and study whether these genes exist mutant. Methods: Designed a pair of specific primers and amplified the exon 3 of eck gene fragment from the extracted genomic DNA derived from normal epithelial cells from skin tissue and ZR-75-1 cell line respectively by PCR technique. Transformed the E. coil. JM109 with recombinant plamids constructed by inserting the amplified fragments into medium vector pUCm-T and sequenced these amplified fragments after primary screening of endonuclease restriction digestion and PCR amplification. Results: (1) Obtained the genomic DNA of human normal epithelial cells and ZR-75-1 cell line respectively. (2) Obtained the amplified fragments of human exon 3 of eck gene through PCR technique. (3) Obtained the cloning vectors of exon 3 of eck gene of human normal epithelial cells and ZR-75-1 cell line respectively. (4) ZR-75-1 cell line exists mutation of nucleotides. Conclusion: Successfully established the method of cloning the human exon 3 of eck gene and found some mutations in the detected samples. This study lays a foundation for further studying the function of eck gene in tumorgenesis.

9

Cite

Share

Study on the effect of T-2 toxin and selenium on CD44 expression in the cultured human fetal chondrocytes in vitro

Long XIE

Academic Journal of Xi'an Jiaotong University.2003;15(1):78-81.

Objective: To investigate the effect on the structure of reestablished cartilage in vitro and CD44 expression on chondrocytes and compare the inducing effect on the reestablished cartilage in vitro between cortical bone matrix gelatin and cancellous bone matrix gelatin. Methods: To plant human fetal chondrocytes on the BMG, the damage of the cultured chondrocytes was observed by the optical microscope (HE staining). The immunohistochemistry of CD44 was quantitative analysis by the image collection and analysis system. Results: With the increasing concentraton of T-2 toxin, the damage of chondroytes was more and more evident and CD44 expression was lowered. After adding selenium, the damage was relieved and CD44 expression increased. The density of chondrocytes on the cortical bone matrix gelatin was much higher than that on the cancellous bone matrix gelatin. Conclusion: T-2 toxin can lower the CD44 expression on the chondrocytes and adding selenium can relieve the damage caused by T-2toxin and increased CD44 expression. The inducing effect on reestablished cartilage in vitro of cortical bone matrix gelatin was much higher than that of cancellous bone matrix gelatin.

10

Cite

Share

Expression and significance of basic fibroblast gwowth factor and fibroblast growth factor receptor-1 in ovarian epithelial neoplasm

Shangfeng GAO

Academic Journal of Xi'an Jiaotong University.2003;15(1):82-85.

Objective: To study the relevance of expression of basic fibroblast growth factor (bFGF), fibroblast growth factor receptor-1 (FGFR-1) and carcinogenesis and progression of ovarian epithelial neoplasm. Methods: Ten cases of normal ovarian tissues and 75 cases of ovarian epithelial neoplasm tissues were detected by immunohistochemical methods: S-P for bFGF, FGFR-1, double immunohistochemistry Lab-SA for Ki-67 antigen and bFGF. Results: The expression level of bFGF, FGFR-1 in ovarian epithelium and ovarian epithelial neoplasm showed a step-wise increase in the following order: normal 〈benign 〈borderline 〈malignant; The expression level and intensity of bFGF and FGFR-1 were increased with the decrease of differentiation degree and increase of clinical stage in ovarian carcinoma; There was no statistical difference between the expression of bFGF, FGFR-1 in serous cystadenocarcinoma and that of mucinous cystadenocarcinoma; The expression of bFGF was correlated with that of FGFR-1 in neoplastic tissues; There were positive expression rates of bFGF and Ki-67 antigen in ovarian epithelial neoplasm. Conclusion: As an important proliferative factor, bFGF plays an important role in carcinogenisis and progression of ovarian epithelial neoplasm.

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share