Objective: Molecular cloning and sequencing of the human matured fragment of human nerve growth factor(NGF) gene. Methods: Extracting the human genomic DNA from the white blood cells as templates, the gene of NGF was cloned by using PCR and T-vector cloning method. Screening the positive clones and identified by the restriction enzymes, and then the cloned amplified fragment was sequenced and analyzed. Results: DNA sequence comparison the cloned gene of NGF with the GenBank (V01511) sequence demonstrated that both of sequences were identical, 354bp length. Conclusion: Cloning the NGF gene from the human genomic DNA has paved the way for further study on gene therapy of nerve system injury.