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Determination of paeoniflorin in Fufang Biejia Ruangan Tablet by TLC scanning

Mao YI ; Lingyun NIE ; Yun LIU ; Ke SONG

Chinese Pharmaceutical Journal.2001;(3):196-198.

OBJECTIVE　To establish a method for the determination of paeoniflorin in Fufang Biejia Ruangan tablet.METHODS　The tablets were extracted with 50% ethanol.The sample solution was applied on a silica gel G-CMC plate and developed with chloroform-methanol-glacial acetic acid (85∶13∶2) and was sprayed with sulfuric acid containing 5% vanillin.The separated spot was determined by scanning with CS-930 at λS=575 nm,λR=700 nm.RESULTS　The average recovery was 97.60% and the RSD was 3.67%.CONCLUSION　The results showed that this method is sensitive,specific,accurate and reproducible.

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A dual-wavelength spectrophotometry of serum theophylline with concomitant use of ofloxacin

Xian HUANG ; Lingping FANG ; Jianhua XU ; Fenghui YOU ; Xiaofang ZENG

Chinese Pharmaceutical Journal.2001;(3):194-196.

OBJECTIVE　To develop a method for the determination of theophylline in serum with concomitant use of ofloxacin.METHODS　Theophylline was serum was extracted with chloroform-hexane(7∶3,v/v) after some ammonium sulfate was added.It was re-extracted with NaOH solution (0.1 mol*L-1)and detected with a UV spectrophotometer at 275 nm and 299.5 nm respeitively.RESULTS　The method was linear over the range of 5.0～40.0 mg*L-1(r=0.9997,n=7).The average recovery of methodology was 100.3%(RSD=1.2%),and the extraction recovery from serum was 87.7%(RSD=4.6%).CONCLUSION　This practical method for the determination of serum theophylline can eliminate the interference of ofloxacin and other antibiotics.

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Study on the preparation of piroxicam films

Ping ZHOU

Chinese Pharmaceutical Journal.2001;(3):172-173.

OBJECTIVE　To prepare the piroxicam films and study its characteristics of transdermal delivery.METHODS　Different high polymers were employed to prepare piroxicam films.Rat abdomen skin and modified Franz diffusion cells were used in the drug penetration tests and release tests.The percutaneous penetration rate was investigated.RESULTS　The release rate of piroxicam films was 64.31 μg*cm-2*h-1.The in vitro permeation rate was 14.86 μg*cm-2*h-1.The percutaneous penetration rate was 11.94 μg*cm-2*h-1.CONCLUSION　Piroxicam films was developed.

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Identification of proteins and polypeptides from Fructus Lycii by HPCE

Zhiliang CHEN ; Zhende CHEN ; Lianbing HOU ; Zhongyuan XU ; Jianwu LIU ; Xixiao YANG

Chinese Pharmaceutical Journal.2001;(3):192-194.

BJECTIVE　To identify the fruits of Lycium barbarum L.(LB) and Lycium chinense Mill.(LC).METHODS　The proteins and polypeptides from the fruits of LB and LC were analysed by high-performance capillary electrophoresis.RESULTS　LB could be distinguished from LC by their electrophorograms.CONCLUSION　HPCE could be used for the pharmacognostic identification of Fructus Lycii.

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Determination of propofol and lidocaine in human plasma by RP-HPLC

Qunlin WU ; Jie WANG ; Ruosong WANG ; Xingan ZHANG

Chinese Pharmaceutical Journal.2001;(4):271-274.

OBJECTIVE　To establish an assay for the simultaneous determination of propofol and lidocaine in human plasma using reversed-phase high performance liquid chromatography（RP-HPLC）.METHODS　With thymol and bupivacaine as the internal standards for propofol and lidocaine respectively,the extraction was performed with cyclohexane.The organic layer was evaporated and the residue was redissolved by mobile phase.The concentrations of propofol and lidocaine were assayed on a hypersil BDS C18 column with a mobile phase consisting of acetonitrile-methanol-water（10∶60∶30）（including 0.14% n-butyamine 0.1% acetic acid）at a flow rate of 1 mL*min-1 and detected at 220 nm during 1～7 min and at 273 nm during 7～16 min.RESULTS　The linearity between concentrations and peak area ratio was obtained from 0.1 μg*mL-1 to 25.6 μg*mL-1（r=0.998，r=0.9995）.The detection limits were 0.1 μg*mL-1 for propofol and 0.05 μg*mL-1 for lidocaine.The within-day and between-day variation coefficients were less than 5%(n=5).The average recoveries were 93.33%,99.4% and 90%,95.67% respectively.CONCLUSION　The method was found to be rapid,accurate and precise.It is suitable for clinical pharmacokinetic and pharmacodynamic study.

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Determination of uric dopamine content by HPLC-RF for patients with Parkinsons disease treated with laser acupuncture

Cangsang SONG ; Shan LIN ; Peilin ZHANG ; Qin ZHANG ; Zhongkun LI ; Renqiu LI

Chinese Pharmaceutical Journal.2001;(4):269-271.

OBJECTIVE　To determine the concentrations of dopamine (DA) in human urine,studying the change of uric dopamine content before and after laser acupuncture treated Parkinsons disease patients.To estabilsh attemptly the standard of apprasing the treatment method which is combined traditional medical theory with modern technology.METHODS　The urine samples were extracted with organic solvent and determined by HPLC-RF.RESULTS　A good correlation was obtained between dopamine over 20～400 ng*mL-1 and peak height,r=0.9996.The recoveries of DA were (89.69±3.95)%～(92.99±6.5)% for urine samples.There was a close correlation between DA content and the severity of parkinsons disease.The DA content in urine of patients before acupuncture was less than healthy ones.DA content in urine increased most at 4 h and content still maintained high as normal scope at 12 after acupuncture.CONCLUSION　This HPLC-RF assay was sensitive,accurate stable and convenient.It is an important method to evaluate the effects of treated Parkinsons disease by laser acupunctrue.

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Clinical comparison of cerivastatin with simvastatin for aged hyperlipidemia

Zhe CHEN ; Jian ZHANG

Chinese Pharmaceutical Journal.2001;(4):276-277.

OBJECTIVE　To assess the efficacy and safety of Lipobay (cerivastatin) and Jingbishuxin (simvastatin) on lipid profiles.METHODS　40 patients with hypercholesterolemia were randomizedly divided into Lipobay group (0.3 mg,QN) and Jingbishuxin group (domestic simvastatin tablet) (20 mg,QN)for 4 weeks.Total cholesterol (TC),low density Lipoprotein cholesterol (LDL-C),triglyceride (TG) and high density lipoprotein cholesterol (HDL-C),blood sugar,hepatic and renal function,blood and urine regular test were measured before and after the treatment.RESULTS　After 4 weeks of treatment,TC,LDL-C and TG were reduced by 29.02%,29.95%,17.21% in Lipobay the group and 22.48%,28.68%,14.74% in Jingbishuxin group,respectively.Compared the measurements after treatment with that before treatment,there were significant differences in both groups,but there was no significant difference between them.No serious adverse effect was found.CONCLUSION　Lipobay had a same efficacy and safety on lipoprotein profiles as that of Jingbishuxin in elderly.It has a good tolerance with smaller dose.

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Determination of the content of berberine hydrochloride tablets by potassium dichromate colormetric method

Jianmin HOU ; Qun LIU ; Aiping FENG

Chinese Pharmaceutical Journal.2001;(4):274-275.

OBJECTIVE　To develop an assay for the determination of berberine hydrochloride in berberine hydrochloride tablets,and to compare the results measured by colormetric method with the assay described in Chinese Pharmacopoeia 1995 for assessing the correlation of the two methods.METHODS　Potassium dichromate colormetric method was selected to determine the content, and the 0.2 mg*mL-1Potassium dichromate was used as the reference solution. The absorbency was measured at 421 nm.RESULTS　The calibration curve for berberine hydrochloride was linear within the concentration range of 4.0～28.0 μg*mL-1(r=0.9999).The average recovery was 100.05%(n=5),and the RSD was 0.43%.The analytical results for berberine hydrochloride between the two methods was found to be correlated.CONCLUSION　The method was proved to be simple, precise and reproduciable. It can be used for the quantitative determination of berberine hydrochloride. It is especially practicable for rapid analysis in production.

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Determination of racecadotril and its impurities by HPLC

Zhe ZHAO ; Shuchun LIU

Chinese Pharmaceutical Journal.2001;(4):267-269.

OBJECTIVE　A HPLC method was established for the determination of racecadotril and its impurities.METHODS　A ODS column was used and the mixture of acetonitrile-potassium dihydrogen phosphate (KH2PO4) solution (70∶30) was used as the mobile phase.The detection wavelength was 210 nm.RESULTS　The liner range of the racecadotril was 0.08～0.24 mg*mL-1 and the regression equation was Y=15847X+3873(r=0.9997).The liner range of benzylthiorphan disulphide was 2.40～21.56 μg*mL-1 and the regression equation was Y=1826x+46 (r=0.9999).The measurable lowest limit was 1 μg*mL-1.The average recovery was 100.0%.CONCLUSION　The method was convenient,accurate and specific.

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Determination of magnolol and honokiol in cortex magnoliae officinalis and their supercritical fluid extract by capillary zone electrophoresis

Guifang WANG ; Shouyao ZHANG ; Zhongyi ZHANG ; Zhengjie LEI

Chinese Pharmaceutical Journal.2001;(4):265-267.

OBJECTIVE　To establish a CZE method for the simultaneous determination of magnolol and honokiol in cortex magnoliae officinalis and their supercritical fluid extract.METHOD　The buffer solution was 20 mmol*L-1 sodium borate(pH=10),the internal standard was sulfamethoxazole and the detection wavelength was 294 nm.RESULTS　Magnolol and honokiol in cortex magnoliae officinalis and their supercritical fluid extract were separated completely and determined accurately.The recoveries and the relative standard deviation were 99.40% and 1.6% for magnolol,and 98.44% and 1.4% for honokiol (n=3) respectively.CONCLUSION　The method was simple and accurate and it can be used for the quality control of magnolol and honokiol in cortex magnoliae officinalis and their supercritical fluid extract.

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