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Rheological properties of xanthan gum solution and its suspending effect

Chinese Pharmaceutical Journal.1999;(4):250-.

OBJECTIVE:To determine the rheological properties of xanthan gum and to investigate its suspending effect.METHODS:Under different conditions such as light,refrigeration and additives,the NDJ-1 rotative viscometry was used to measure the change of viscosity by various rates of shearing.We used sucralfate as model drug,xanthan gum as suspension agent,and prepared 100g.L-1 of sucralfate suspesion.RESULTS:Plastic construction and thixotropy appeared in the solutions.Its optimum concentration for suspension was 3g.L-1 and this suspension appeared significant thixotropy.CONCLUSION:Some factors such as light,refrigeration and additives have no effects on thixotropy.The solution of xanthan gum can be applied as suspension agent.

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Effects of ligustrazine on serum CGRP and on the expression of CGRP,PDGF and bFGF in lung of hypoxic rats

Taiqin TANG ; Zhihong HUANG ; Shengming LIU ; Nasi HUANG

Chinese Pharmaceutical Journal.1999;(4):237-.

OBJECTIVE:To study the effects of ligustrazine on the SCGRP and on the expression of CGRP,PDGF and bFGF in the lung of CHPH rats.METHOD:A stable rat modle of CHPH was established.The serum CGRP levels were mesured by enzyme immunoassay.The expression of CGRP,PDGF and bFGF in lumg were observed by immunohistochemical method.RESULTS:①The SCGRP level of H group was significantly lower than that of N group (P＜0.01) but no difference between the H+Li group and H group (P＞0.05).②The expression of CGRP,PDGF and bFGF increased obviously in lung tissue of H group,whereas the expression of bFGF and PDGF of H+Li group reduced obviously but that of CGRP was not influenced.CONCLUSION:SCGRP reduction and expression increasion increasion of PDGF.bFGF have close relation with the formation of CHPH;Inhibiting the expression of PDGF.bFGF may be an aspect of the mechanism of preventing and treating HPH by ligustrazine.

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The secretion of catecholamine in cultured rat adrenal medullary chromaffin cells measured by high-performance liquid chromatography-electrochemical detection assay

Ling SONG ; Junmin YANG

Chinese Pharmaceutical Journal.1999;(3):172-.

OBJECTIVE：To study the secretion of catecholamine primary cultures of chromaffin cells from rat adrenal medulla were used.METHOD:Catechoalmines(norepinephrine,epinephrine and dopamine) were measured by high-performance liquid chromatography-electrochemical detection technique.RESULTS:Catecholamine released by chromaffin cells with in 20min without slimalus was (73.29±15.32) ng/106 cells.When acetylcholine,nicotine or muscarine was added,the secretion of catecholamine was then increased.CONCLUSION:Using high-performance liquid chromatography-electrochemical detection technique,we can detect sensitively catecholamine released by cultured rat chromaffin cells.

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Isolation purification and characterization of NV-PP-1 from Nidus vespae

Lin LI ; Xuemei LIU

Chinese Pharmaceutical Journal.1999;(4):233-.

OBJECTIVES:To isolate and purify a new peptide named as NV-PP-1,from anti-inflammatory protein extracts of Nidus Vespae and to characterize it.METHODS:It was purified by ammonium sulfate precipitation,DEAE-Sephadex A50 ionexchange chromatography,Sephadex G-50 gel filtration and HPLC，respectively.Then it was characterized by HPLC on Protein-PakTM-60 column,IEF-HPCE(isoelectric focusing-high performance capillary electrophoresis),UV spectrum,amino acid analysis,and the N-terminal analysis with DNS-Cl.RESULT:Both HPLC on Protein-PakTM-60 column and IEF-HPCE showed its homogeneity.Its molecular weight was estimated to be 7.079 kD by HPSEC.UV spectrum indicated its maximum absorption at 274nm.Its PI was determined to be 4.08 according to IEF-HPCE.The terminal analysis showed its N-terminal amino acid was blocked.Amino acid analysis of NV-PP-1 indicated that it was rich in Ser,Glu,Gly.CONCLUSION:NV-PP-1 is the first peptide isolated from the anti-inflammatory protein extracts of Nidus vespae.

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Anti-asthmatic effect inhibition of artesunate on the asthmatic model of guinea-pig

Maosheng YANG ; Kang HE ; Weiming XU

Chinese Pharmaceutical Journal.1999;(3):160-.

OBJECTIVE：To study the anti-asthmatic effects of artesunate on the durg-induced asthmat.METHOD:Drug-induced asthmatic model of guinea-pig. RESULTS:Using nubilous inhalation or intragastric administration,artesunate significantly inhibited the guinea-pig model asthma in a dose-dependent manner.It's ED50 were (99.95±7.69)mg.L-1(n=4) and (15.64±1.25)mg.kg-1.d-1(n=4) respectively. CONCLUSION:artesunate showed anti-athmatic effect in guinea-pig asthmatic model.

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Preliminary study of rare-earth elements on the growth of Ganodema lucidum

Liqiong GUO ; Liqing GUO

Chinese Pharmaceutical Journal.1999;(3):155-.

OBJECTIVE：Rare-earth compound was added to the medium of Ganoderma lucidum to study the effect of rare-earth elements on the hypha growth,the fresh and dry weight of fruit body of G.lucidum.METHODS:The culture medium dressed with rare-earth elements.RESULTS:It showed that all concentrations of rare-earth elements had some inhibition on the hypha growth of G.lucidum.However they could make the hypha of G.lucidumwhiter.The fresh and dry weight of fruit body increased significantly when the concentrations of rare-earth elements were 100μg.g-1 and 150μg.g-1.Their raising rates were +4.13%，+0.20% and +16.47，+5.84%respectively.CONCLUSION:The yield of G.lucidum can be increased by rare-earth elements when its concentrations were 100～150μg.g-1.

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The preparation of ketoconazole eye drops

Shumei ZHANG

Chinese Pharmaceutical Journal.1998;(4):223-.

OBJECTIVE: To extend clinical application scope of ketoconazole, and satisfy the needs of ophthalmologists with mycotic infection. METHODS: Polysorbate 80 was used as solubilization, acidic water dissolent, basic water pH regulator, anhydrous sodium bisufite stabilizer, and ethylparaben preservative. The eye drops were prepared according to the ordinary method of manufacturing. RESULTS: The eye drops have good stability without stimulation to eye. Its quality can by controlled for good curative effect. CONCLUSION: Ketoconazole could be prepared eye drops for clinical application.

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Therapeutic effect of Cili juice enriched with SOD on lead poisoning in rats and mice

Lin YANG ; Shufang LI ; Bingnan XIA

Chinese Pharmaceutical Journal.1998;(4):211-.

OBJECTIVE: To observe the effects of Cili juice enriched with SOD (CLJES) on lead excretion, activity of superoxide dismutase (SOD) in erythrocytes, plasma lipid peroxidation (LPO) and immune function in experimental lead poisoning animals. METHODS: Lead poisoning models were made in rats and mice. The effects of CLJES on lead excretion, SOD activity, LPO concentration in rats and immune functions in mice were determined and compared with those of conventional EDTA treatment. RESULTS: CLJES significantly increased lead excretion. Furthermore it significantly increased SOD activity, reduced LPO concentration in rats with lead poisoning and enhanced immune function in lead loaded mice, however EDTA had no such actions. CONCLUSION: CLJES might exert a wider range of therapeutic effects on lead poisoning than EDTA.

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Oily constituents of Meconopsis punicea

Xuan PAN

Chinese Pharmaceutical Journal.1998;(4):208-.

OBJECTIVE: To analyse oily constituents of Meconopsis punicea Maxim. METHOD: The oily constituents were analysed and identified by GC-MS. RESULTS: The sixty-three constituents were isolated and the forty-seven of that were identified, making up 74.6% of the total constituents. Ester compounds accounted for 57.5% of the identified compounds. The main constituents were ester octadecnate. CONCLUSION: Ester octadecenate has high biological activity, which agrees with curative effect of the plant.

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Study on the quality standard for Liyan granule

Xinmei CHEN ; Xihong GUO

Chinese Pharmaceutical Journal.2001;(2):125-127.

OBJECTIVE　To establish a quality standard for Liyan granule.METHODS　Honeysukle flower in the granule was identified by TLC.The developing agent was the upper solvent of butyl acetate formil acid-water(7∶2.5∶2.5).The dual wavelength spectrophotometry was applied for the determination of chlorogenic acid in granule.And the acute toxicity test was accomplished.RESULT　The TLC for the identification of Honeysule flower in the granule was accurate.The uv for chlorogenic acid was reliable.The linear range of chlorogenic acid was 0.46～46.00 μg*mL-1(r=0.9992),and the recovery was 97.33%,RSD=1.49%.The acute toxicity test showed that the maximal tolerable dose in rats was equivalent to 850 times of the clinical dosage in human.CONCLUSION　This method could be used as the quality control during the production of Liyan granule.

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