Chinese Pharmaceutical Journal 2001;(3):196-198

Determination of paeoniflorin in Fufang Biejia Ruangan Tablet by TLC scanning

Mao YI ; Lingyun NIE ; Yun LIU ; Ke SONG

Country

China

Language

Chinese

Abstract

OBJECTIVE To establish a method for the determination of paeoniflorin in Fufang Biejia Ruangan tablet.METHODS The tablets were extracted with 50% ethanol.The sample solution was applied on a silica gel G-CMC plate and developed with chloroform-methanol-glacial acetic acid (85∶13∶2) and was sprayed with sulfuric acid containing 5% vanillin.The separated spot was determined by scanning with CS-930 at λS=575 nm,λR=700 nm.RESULTS The average recovery was 97.60% and the RSD was 3.67%.CONCLUSION The results showed that this method is sensitive,specific,accurate and reproducible.