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Extraction of Magnetosome from Acidthiobacillus ferrooxidans

Jianping XIE ; Xinxing LIU ; Wenbin LIU ; Guanzhou QIU

Progress in Modern Biomedicine.2005;5(3):7-10. doi:10.3969/j.issn.1673-6273.2005.03.002

In physiological property and growing environment aspects, there are some similarities between Acidthiobacillus ferrooxidans(ATF) and magnetotactic bacteria. ATF shows magnetotacxis under the microscope. The magnetosome can be extracted from ATF by using the extracting method of magnetosome from magnetotactic bacteria. The membrane of ATF was crushed by ultrasonic wave and the magnetosomes in the ATF which mainly contains Fe through chemical detection were attracted by using magnetite. After purifing with sucrose density gradient centrifugation and washing by PBS, the magnetosomes can be seen clearly through a transmission electron microscope. The results indicate there are a small amount of intracellular magnetosomes which made the ATF be magnetotactic under the applied magnetic field. It is the first time to find that ATF was magnetotactic. The magnetotaxis of ATF can be utilized and isolated by the magnetotactic properties to gain the high-performance ATF strains of mineral leaching, which have high activity and different magnetism.

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Effects of extremely low frequency magnetic field on growth kinetics, metabolism and cell cycle of human liver cancer cells

Liang DONG ; Lingzhen HUANG ; Yantian CHEN ; Tao GENG ; Dengming XIAO ; Hanshi QI

Progress in Modern Biomedicine.2005;5(3):1-6. doi:10.3969/j.issn.1673-6273.2005.03.001

Objective: To study the effects of extremely low frequency (ELF) magnetic field with fixed parameters on human liver cancer cells (SK-HEP-1) at different aspects. Methods: SK-HEP-1 cells were exposed to 50Hz, 20mT magnetic field during the whole culture process, and then proliferation activity, growth kinetics, metabolic profile and cell cycle were analyzed. Results: 50Hz, 20mT magnetic field inhibits the growth and metabolism of SK-HEP-1 cells, and hampers their mitotic division. Conclusion: 50Hz, 20mT magnetic field could be a potential therapy in the treatment of human malignant tumors.

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"The""modification""Type Microsatellite Change in High Frequency Microsatellite Instability Colorectal Cancer Closely Relating to MLH1 and KRAS Mutation"

Yan ZHAO ; Tao ZHANG ; Jianjun ZHANG ; Zhichao ZHENG ; Yiliang ZHAO ; Yoshihiko MAEHARA ; Huimian XIU

Progress in Modern Biomedicine.2008;8(5):875-880. doi:10.3969/j.issn.1673-6273.2008.05.023

Microsatellite instability(MSI)was defined according to the frequency of positive findings in a panel of MSI markers.High frequency MSI(MSI-H)was the phenotype in which repeat sequences were extraordinarily unstable, and was considered to be the bona fide phenotype of DNA mismatch repair defection. However base substitutions in some well studied oncogenes or tumor suppressors were reported to be uncommon in MSI-H tumors. To explore this obvious contradiction, the relationship between MSI and KRAS gene mutations were studied in a panel of 76 human colorectal carcinomas, the whole exon of MLH1 and MSH2 were sequenced for MSI-H tumors. KRAS gene mutation was confirmed by similar frequencies in tumors of different MSI status. Intriguingly, all of the KRAS mutant MSI-H tumors harbored sequence alterations in MLH1gene, which was a key player in DNA mismatch repair system. This implied that in MSI-H tumors carrying MMR mutations, KRAS mutation were frequently and almost exclusively occurred. Furthermore, these MMR mutants were uniformly carrying a unique modification + jumping type MSI, which was different to MSI-H tumors without MLH1 or MSH2 gene mutations. This study shaded lights on the heterogeneity of MSI-H tumors, and implied the connection between modification type MSI and DNA mismatch defection.

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Application of Nonlinear Dynamics in Analysis of Electroencephalograph

Ling HE ; Hua HUANG

Progress in Modern Biomedicine.2006;6(9):24-26. doi:10.3969/j.issn.1673-6273.2006.09.008

Nonlinear dynamics has been used to analyse Electroencephalograph (EEG) time series,in recent years,which has opened new possibilities to the dynamic knowledge of the brain. In this paper, we review researches on the treatment of EEG (sleep stage, anesthesia, process of cognition, schizophrenia, dementia and epilepsy) using nonlinear dynamics methods, and try to better understand brain neurodynamics.

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The Morphology of Vesicles of Higher Topological genus

Li-Qiang XIE ; Fei ZHENG ; Jie-Xi ZHU ; Shao-Guang ZHANG

Progress in Modern Biomedicine.2006;6(9):66-68. doi:10.3969/j.issn.1673-6273.2006.09.025

The equilibrium configuration of fluid-phase phospholipid vesicles in aqueous solutions are controlled by bending elasticity. We exploited a method for calculating explicitly the stability of arbitrary symmetric shapes. The morphology of lipid vesicles with topological genus g=2, i.e. with two holes or two handles, is studied. We provided some stable shapes with results explicitly.

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EEG Characteristics in Frequency Domain in Synaptic Dysfunction Rat Model

Li-Ying HAN ; Xin TIAN

Progress in Modern Biomedicine.2006;6(10):1-4. doi:10.3969/j.issn.1673-6273.2006.10.001

EEG characteristics in frequency domain were investigated in the frontal lobe, occipital lobe and hippocampus, i.e. cognition - related cortex of synaptic dysfunction model rat, providing electrophysiological basis for further study on plastic extent and nerve regeneration of the damaged neurons. Synaptic dysfunction model was made via microinjecting β - amyloid protein1 - 40 (Aβ1 - 40) into hippocampal CA1 area of rat. Morris water maze behavioral test was performed to evaluate the learning and memory function of model group. Then EEG in the above areas for two groups were recorded. The spectrum for two groups was performed and the characteristics in frequency domain were analyzed. The results showed: (1) The average escape latency in 3rd, 4th, 5th and 6th training times of model group are higher than those of normal. The average escape latency of normal group in 5th training time decreased more markedly than that in 2nd training time, while that of model group in 7th training time decreased more remarkably than that in 2nd training time (P ＜0.05). Without platform, the platform quadrant time percentage of model group was lower than the control (P ＜0.05). (2) Alpha rhythm in EEG of model rat was slowing down; alpha- band power decreased with peek frequency left shifted nearly 2Hz. And the power of delta - band and theta - band in frontal lobe, occipital and hippocampus all increased with different extent. The synaptic dysfunction model rats were made successfully by microinjecting Aβ1 - 40 method. The model rats show the decreased learning and memory dysfunction. EEG frequency spectrum features in model rat show slower alpha rhythm with power amplitude lower or loss, slow waves (delta and theta wave) increasing with higher power amplitude. These can be consistent with the EEG of Alzheimer's disease patients, which can provide electrophysiological basis for further plasticity and nerve regeneration study on the impaired cortex with synaptic dysfunction.

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Genetic variants in the tumor necrosis factor receptor 2 gene in patients with Systemic lupus erythematosus

Zhi-Hua YIN ; Zhi-Zhong YE ; Jun-Han ZHUANG ; Da-Qiang GAN ; Xun WANG ; Xiu-Xia LUO

Progress in Modern Biomedicine.2006;6(10):34-36. doi:10.3969/j.issn.1673-6273.2006.10.011

In this paper, we designed to investigate the frequencies of tumor necrosis factor receptor 2 (TNFR2) polymorphisms at nt587 and nt694 in south Chinese SLE patients and healthy individuals and explore whether genetic variants in TNFR2 gene is involved in the pathogenesis of SLE. The results showed that the nt587G allele frequency was 21.1% in the 128 SLE patients and the allele frequency was 13.0% in the 135 healthy individuals, the former was significantly higher than the latter in the allele frequency (P ＜ 0.05). People with the nt587 G variant showed high risk to SLE. The frequency of nt694 was slightly but not statistically significantly increased in SLE patients compared with healthy controls(16.0% versus 11.9%, P= 0.149). These results indicate that the polymorphism at nt587 of TNFR2 is associated with the south Chinese SLE patients. The polymorphism at nt694 is not associated with SLE.

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Progress of animal embryo technology in China

Li-Li WEI ; Xu-Ying WANG ; Ya-Dong YAN ; Bao-Can WANG ; Qing-Fu YAN ; Zhen-Dong LUO ; Zheng-Fu LI ; He LI ; Bing HONG ; Jing ZHOU

Progress in Modern Biomedicine.2006;6(10):100-103. doi:10.3969/j.issn.1673-6273.2006.10.035

Embryo technology, including super-ovulation, embryo transfer and embryo nucleus transfer, was a well-developed technology in 20th century. It has been widely used in animal science and veterinary and boomed livestock industry in China. Chinese livestock producer could gain high grade breeds in short time through embryo technology by the reason that embryo technology could make outstanding gene spread out in herd. With the help of embryo technology, rare animals could have a chance to extend their progeny in fast changing world. In this article, we briefly introduced the process of embryo transfer and application of embryo technology in China.

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Culture Conditions Optimization of Magnetotactic Bacteria WM-1

Wen-Bing LI ; Peng-Peng ZHOU ; Long-Jiang YU

Progress in Modern Biomedicine.2006;6(12):102-105. doi:10.3969/j.issn.1673-6273.2006.12.042

The culture of Magnetospirillum magneticum WM-1 depends on several control factors that have great effect on the magnetic cells concentration. Investigation into the optimal culture conditions needs a large number of experiments. So it is desirable to minimize the number of experiments and maximize the information gained from them. The orthogonal design of experiments and mathematical statistical method are considered as effective methods to optimize the culture condition of magnetotactic bacteria WM-1 for high magnetic cells concentration. The effects of the four factors, such as pH value of medium, oxygen concentration of gas phase in the serum bottle, C:C (mtartaric acid: msuccinic acid) ratio and NaNO3 concentration, are simultaneously investigated by only sixteen experiments through the orthogonal design L16(44) method. The optimal culture condition is obtained. At the optimal culture condition (pH 7.0, a oxygen concentration 4.0%, C: C (mtartaric acid: msuccinic acid) ratio 1:2 and NaNO3 100 mg l-1), the magnetic cells concentration is promoted to 6.5×107 cells ml-1, approximately 8.3% higher than that under the initial conditions. The pH value of medium is very important factor for magnetic cells concentration. It can be proved that the orthogonal design of experiment is of 90% confidence. The results from the hysteresis of WM-1 shows that Hc = 230 Oe, Ms = 0.9 emu/g dry wt. Cells,and Mr / Ms = 0.50.

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The Subsystems of Visual Mental Imagery Processing

Dan-Ni SUI ; Chang-Jun LI ; Li ZHOU ; Qing-Bao YU ; Yi-Yuan TANG

Progress in Modern Biomedicine.2007;7(2):161-165. doi:10.3969/j.issn.1673-6273.2007.02.001

Background mental imagery is a multi-componential process involving different mental operations. Objective The present study investigated the subsystems of visual mental imagery processing and their interactions. Methods: Four experimental tasks were done: (1) object, the ability to generate global mental images; (2) resolution, the ability to generate vivid,high-resolution mental images; (3) transformation,the ability to mentally rotate patterns in images, and (4) spatial imagery,spatial cognition. 91 university students (77 males, 14 females) participated in this study. Results: Object task was highly correlated with resolution task, and the same the tendency was found between transformation task and spatial task. Conclusion: The results indicate there are different subsystems of visual mental imagery and corresponding operative strategies, but with strong correlation among these processes and abilities.

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