Treatment of hypospadias by mobilization of the anterior urethra and meatal advancement
Journal of Zhejiang University. Medical sciences.2002;31(1):53-54.
Journal of Zhejiang University. Medical sciences
2002 (v1, n1) to Present ISSN: 1671-8925
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Serum interleukin-6 levels of appendicitis in children
Journal of Zhejiang University. Medical sciences.2002;31(1):51-52.
Study on application of PCR in the diagnosis of Haemophilus influenzae pneumonia
Journal of Zhejiang University. Medical sciences.2002;31(1):47-50.
OBJECTIVE: To study the role of Haemophilus influenzae(Hi) in pneumonia and that of PCR in the diagnosis of Hi pneumonia. METHODS: Hi genus-specific PCR, Hib type-specific PCR and selective Hi culture media were used to detect 83 samples of deep nasal pharyngeal aspiration (NPA), 51 sera from 83 children younger than 3 years with pneumonia and 37 samples of pharyngeal swabs from healthy children. RESULTS: Of 83 NPA samples, 20(24.1 %) were positive by culture, 36 (43.4 %) positive by Hi-PCR and 19 (22.9 %) positive by Hib-PCR.Six out of 51 sera were positive by Hi-PCR and Hib-PCR, but none positive by culture. Of 37 pharyngeal swabs from healthy children, 3 ( 8.1 %) were positive by culture, 6 ( 16.2 %) positive by Hi-PCR and none positive by Hib-PCR. CONCLUSION: Hib-PCR is more appropriate for detecting NPA samples from children with pneumonia because of the high rate of non-typeable Hi carriers in healthy children.
Preparation of albendazole sulfoxide--- an active metabolite
Journal of Zhejiang University. Medical sciences.2002;31(1):45-46.
OBJECTIVE: To investigate the facile method for preparation of albendazole sulfoxide, an active metabolite in vivo. METHODS: The reaction condition was investigated according to evaluations to the results of the experiment through temperature, solvent, oxidation agent, reaction time and impurity. RESULTS: The target compound was obtained by oxidation of albendazole with the presence of sodium metaperiodate in glacial acetic acid under low temperature and was free from contamination by impurity albendazole sulfone. Pure product (content >98%) and high yield (>90%percnt;) have been achieved due to reaction selection. CONCLUSION: Oxidation condition is essential to the preparation of albendazole sulfoxide.
Relation between formation time and structure of red blood cell aggregation body
Journal of Zhejiang University. Medical sciences.2002;31(1):41-44.
OBJECTIVE: To study the relation between of forming time red blood cell aggregation body and structure by changing the thickness of flow channel plane and red blood cell hematocrit. METHODS: A system with flow channel technique and light transmission measurements were applied to gaining red blood cell aggregation curve. RESULTS: The forming time of red blood cell aggregation body and the thickness of aggregation body as well as red blood cell hematocrit were linearily correlated with a correlation coefficient gamma of 0.989 and 0.990 respectively. CONCLUSION: When red blood cell aggregation process is studied using different methods, red blood cell aggregation time index can not be compared.
Effect of adriamycin on sarcoplasmic reticulum Ca2+ -ATPase activity in cardiomyocyte of rabbits
Journal of Zhejiang University. Medical sciences.2002;31(1):37-40.
OBJECTIVE: To evaluate influences of regular-dose of adriamycin (ADR) on heart function and sarcoplasmic reticulum (SR) Ca2+ -ATPase in cardiomyocyte of rabbits. METHODS: Nine rabbits received intraveneous injection of ADR (2mg/kg) once a week for 8 weeks, the rabbits injected with saline were used as control group. Cardiac output (CO), blood pressure (BP), mean artery pressure (MAP), left ventricular systolic pressure( LVSP), left ventricular end-diastolic pressure (LVEDP), calcium in cardiomyocyte (MyoCa2+) of rabbits and SR Ca2+ -ATPase activity were examinated 3 weeks after the final injection. RESULTS: CO, LVSP and SR Ca2+ -ATPase activity were significantly decreased in ADR treated group compared with the control group. Conversely, LVEDP and MyoCa2+ were significantly increased in ADR treated rabbits. CONCLUSION: Heart function can be decreased by regular-dose of ADR in injection. Calcium overload in cardiomyocyte and decrease of SR Ca2+ -ATPase activity is important physiopathologic mechanism in ADR-induced impairment of heart.
Effects of liposomal prostaglandin E(1) on myocardial reperfusion injury in rabbits
Journal of Zhejiang University. Medical sciences.2002;31(1):33-36.
OBJECTIVE: To probe into the role of liposomal prostaglandin E(1) (Lipo-PGE(1)) on reperfusion injury in a rabbit ischemia-reperfusion model. METHODS: Twenty-four open-chest rebbits were randomized to receive a 10 min intravenous infusion of either liposome diluent, free PGE(1), or Lipo-PGE(1) after 60 min of left anterior desending (LAD) ligation just before reperfusion. Serum creatine phosphokinase (CPK), malodialdehyde (MDA), superoxide dismutase (SOD) were detected; infarct size and region at risk were measured. RESULTS: Infarct size as a ratio of weight of infarcted tissue to weight at risk (MI/RISK) was significantly reduced with Lipo-PGE(1) (32.20+/-4.70)% compared with PGE(1) (42.09+/-6.93)% or placebo (44.57+/-5.46)% infusion (P<0.01). The values of serum CPK, MDA during reperfusion in treatment of Lipo-PGE(1) group were significantly reduced than in treatment of PGE(1) group or control group (P<0.05), and the values of serum SOD were significantly increased (P<0.05). CONCLUSION: Lipo-PGE(1) can effectively decrease the serum CPK and MDA contents, elevate the SOD activity, and attenuate myocardial reperfusion injury.
High bioactive material-bone graft in bone defect healing
Journal of Zhejiang University. Medical sciences.2002;31(1):30-32.
OBJECTIVE: To assese the value and clinical significance of bioactive material-hydroxyapatite (HA) combined with TGF-beta1 in bone defect healing. METHODS: The applicability of bioactive material-HA granules combined with TGF-beta1 (group A) as a substitute for bone graft was observed in SD rat model. The results were compared with those of HA granules (Group B) and ungrafted bone defect (Group C). RESULTS: Group A demonstrated the highest level of type I collagen mRNA during healing period. CONCLUSION: The bioactive material-HA combined with TGF-beta1 can efficiently bond to ongrowing new bone comparing to HA granules.
Journal of Zhejiang University. Medical sciences.2002;31(1):26-29.
OBJECTIVE: To study the effect of basic fibroblast growth factor(b-FGF) on revascularization and bone remodeling of allogeneic mandible transplantation in repair of mandible defects in rabbits. METHODS: The mandible defects of 20 adult rabbits were created in both sides. The defects on the left side were implanted with allogeneic bone and local administration of b-FGF; the defects on the right side were only repaired with allogeneic bone as control group. At 1, 3 months after operation, the revascularization and bone remodeling were observed by ink-gelation vascular perfusion-transparency and histological examination. RESULTS: The allogeneic bone and b-FGF group had more marked vascularization and more quick and complete bone formation than control group. CONCLUSION: b-FGF can improve revascularization and bone formation after allogeneic mandible transplantation; allogeneic bone combined with b-FGF is a promising bone substitute in clinical uses.
Influence of peritoneal dialysate on nitric oxide production and viability of macrophage
Journal of Zhejiang University. Medical sciences.2002;31(1):23-25.
OBJECTIVE: To investigate the influence of commercial peritoneal dialysate (CDS) on function of macrophage. METHODS: Cultured peritoneal macrophages were divided into two experimental groups and their controls.(1) Macrophages were cultured in conditioned culture medium containing 50%CDS (0.139 mol/L glucose) for 24 h. (2)Macrophage were exposed to CDS containing 0.139mol/L glucose for 10, 30 and 60 min respectively, and then cultured in CDS-free medium for 24 h. The nitric oxide (NO) production and MTT in two groups were measured. In each control group, CDS was replaced by same amount of culture medium. RESULTS: NO production and MTT reduction ability (related to cell viability) of experimental groups were remarkably lower than those of controls and the NO production and MTT reduction in 60min CDS-exposed group were lower than that of 10 min and 30 min CDS-exposed group. CONCLUSION: Dialysate may have detrimental effects on viability and other function of macrophage and these effects may related to time length of CDS exposure.
Country
China
Publisher
浙江大学
ElectronicLinks
http://www.sciengine.com/JZJUMS/homeEditor-in-chief
zdxbyxb@zju.edu.cn
Abbreviation
Journal of Zhejiang University. Medical sciences
Vernacular Journal Title
浙江大学学报·医学版
ISSN
1008-9292
EISSN
Year Approved
2013
Current Indexing Status
Currently Indexed
Start Year
1958
Description
1958-1999(3):浙江医科大学学报; 1999(4)-:浙江大学学报·医学版
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