Journal of Zhejiang University. Medical sciences 2009;38(4):343-347

Fetal bovine serum enhances expression of PEDF in epidermal keratinocytes and dermal fibroblasts.

Xiao-yong MAN 1 ; Yong-gang YAO ; Xiao-hong YANG ; Sui-qing CAI ; Min ZHENG

Affiliations

+expand

Country

China

Language

Chinese

MeSH

Abstract

OBJECTIVETo investigate the effect of fetal bovine serum (FBS) on expression of pigment epithelium-derived factor (PEDF) in normal epidermal keratinocytes and dermal fibroblasts.

METHODSKeratinocytes and fibroblasts were incubated with 10% FBS. PEDF protein level in the cells was determined by immunofluorescence and Western blot.

RESULTSPEDF was localized mostly in the cytoplasm,while some in the nuclei. The distribution of PEDF in cytoplasm was in a granular pattern. 10% FBS increased the expression of PEDF both in keratinocytes and fibroblasts,but histamine and Phorbol 12-myristate 13-acetate (PMA) did not interfere the distribution of PEDF in cells.

CONCLUSION10% FBS can upregulate expression of PEDF in epidermal keratinocytes and dermal fibroblasts.