Journal of Zhejiang University. Medical sciences 2009;38(4):348-351

Effects of 8-Methoxypsoralen on intracellular Ca(2+)i and cytoskeleton actin organization in human melanocytes in vitro.

Xian-qi ZHANG 1 ; Min ZHENG ; Kuan-hou MOU ; Jie FENG

Affiliations

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Country

China

Language

Chinese

Abstract

OBJECTIVETo investigate the effects of 8-methoxypsoralen on human melanocytes [Ca(2+)]i and cytoskeleton actin organization in vitro.

METHODSHuman melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca(2+)]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin.

RESULTS8-methoxypsoralen increased [Ca(2+)]i and induced organization of actin stress fiber cytoskeleton.

CONCLUSION8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca(2+) concentration and cytoskeleton actin reorganization.