Nuclear Medicine and Molecular Imaging.2008;42(5):422-424.
Nuclear Medicine and Molecular Imaging
to Present ISSN: 1975-129X
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A Case of Paranasal Sinus Papilloma with Increased FDG Uptake.
Nuclear Medicine and Molecular Imaging.2008;42(5):419-421.
The false-positive FDG uptakes on head and neck areas are common due to benign lesion, iatrogenic and physiologic changes. The Schneiderian papilloma is uncommon benign tumor arising from the mucosa of the paranasal sinuses and nasal cavity. The findings of paranasal papilloma on conventional modality such as CT and MRI are non-specific and they could be confused with inflammatory polyp or retention cyst. Despite of benign tumor, the papilloma usually shows locally aggressive growth with malignant potential, therefore the FDG can be actively accumulated in this lesion. We describe the case of 18F-FDG PET/CT finding in a 77-year-old woman who demonstrates oncocytic papilloma in maxillary sinus.
Aged ; Female ; Fluorodeoxyglucose F18 ; Head ; Humans ; Mucous Membrane ; Nasal Cavity ; Neck ; Papilloma ; Paranasal Sinuses ; Polyps ; Retention (Psychology)
Aged ; Female ; Fluorodeoxyglucose F18 ; Head ; Humans ; Mucous Membrane ; Nasal Cavity ; Neck ; Papilloma ; Paranasal Sinuses ; Polyps ; Retention (Psychology)
Hepatocellular Carcinoma with Right Atrial Invasion Detected by PET/CT.
Nuclear Medicine and Molecular Imaging.2008;42(5):414-418.
The role of positron emission tomography (PET) with F-18 fluorodeoxyglucose (F-18 FDG) in the diagnosis of hepatocellulcar carcinoma (HCC) has been limited because of a variable FDG uptake in HCC. However, the usefulness of PET/CT for detecting extrahepatic metastasis and monitoring of the treatment response in HCC has been reported. A 55-year-old man with a hepatitis B surface antigen-positive, was admitted to our hospital due to dyspnea, general weakness and body weight loss for one month. Chest X-ray showed multiple reticulo-nodular densities on both lower lung fields, which implies metastatic lesions. F-18 FDG PET/CT revealed consecutively intense hypermetabolic mass in right hepatic lobe, inferior vena cava and right atrium. We report a case of HCC with IVC and right atrium invasion identified by F-18 FDG PET/CT.
Body Weight ; Carcinoma, Hepatocellular ; Dyspnea ; Heart Atria ; Hepatitis B ; Humans ; Lung ; Middle Aged ; Neoplasm Metastasis ; Positron-Emission Tomography ; Thorax ; Vena Cava, Inferior
Body Weight ; Carcinoma, Hepatocellular ; Dyspnea ; Heart Atria ; Hepatitis B ; Humans ; Lung ; Middle Aged ; Neoplasm Metastasis ; Positron-Emission Tomography ; Thorax ; Vena Cava, Inferior
Esophageal Leiomyoma with intense FDG uptake on 18F-FDG PET/CT.
Nuclear Medicine and Molecular Imaging.2008;42(5):410-413.
A 56 years old woman referred to our hospital with dysphagia and epigastric soreness. Gastroendoscopy revealed huge submucosal tumor with ulceration extending from distal esophagus to lesser curvature of stomach. Subsequent computed tomography (CT) demonstrated soft tissue mass encircling distal esophagus, and 18F-FDG PET/CT demonstrated intense 18F-FDG accumulation in it. Finally this case was diagnosed as esophageal leiomyoma based on pathologic evaluation of the surgical specimen.
Deglutition Disorders ; Esophagus ; Female ; Fluorodeoxyglucose F18 ; Humans ; Leiomyoma ; Stomach ; Ulcer
Deglutition Disorders ; Esophagus ; Female ; Fluorodeoxyglucose F18 ; Humans ; Leiomyoma ; Stomach ; Ulcer
Facile Fabrication of Animal-Specific Positioning Molds For Multi-modality Molecular Imaging.
Nuclear Medicine and Molecular Imaging.2008;42(5):401-409.
PURPOSE: Recently multi-modal imaging system has become widely adopted in molecular imaging. We tried to fabricate animal-specific positioning molds for PET/MR fusion imaging using easily available molding clay and rapid foam. The animal-specific positioning molds provide immobilization and reproducible positioning of small animal. Herein, we have compared fiber-based molding clay with rapid foam in fabricating the molds of experimental animal. MATERIALS AND METHODS: The round bottomed-acrylic frame, which fitted into microPET gantry, was prepared at first. The experimental mice was anesthetized and placed on the mold for positioning. Rapid foam and fiber-based clay were used to fabricate the mold. In case of both rapid foam and the clay, the experimental animal needs to be pushed down smoothly into the mold for positioning. However, after the mouse was removed, the fabricated clay needed to be dried completely at 60 degrees C in oven overnight for hardening. Four sealed pipet tips containing [18F]FDG solution were used as fiduciary markers. After injection of [18F]FDG via tail vein, microPET scanning was performed. Successively, MRI scanning was followed in the same animal. RESULTS: Animal-specific positioning molds were fabricated using rapid foam and fiber-based molding clay for multimodality imaging. Functional and anatomical images were obtained with microPET and MRI, respectively. The fused PET/MR images were obtained using freely available AMIDE program. CONCLUSION: Animal-specific molds were successfully prepared using easily available rapid foam, molding clay and disposable pipet tips. Thanks to animal-specific molds, fusion images of PET and MR were co-registered with negligible misalignment.
Aluminum Silicates ; Animals ; Fungi ; Immobilization ; Magnetic Resonance Imaging ; Mice ; Molecular Imaging ; Veins
Aluminum Silicates ; Animals ; Fungi ; Immobilization ; Magnetic Resonance Imaging ; Mice ; Molecular Imaging ; Veins
Nuclear Medicine and Molecular Imaging.2008;42(5):394-400.
PURPOSE: Quantitative comparison of transgene expression within stem cells between lentivirus and adenovirus-mediated delivery systems has not been reported. Here, we evaluated the human sodium iodide symporter (hNIS) gene expression in rat mesenchymal stem cell (rMSC) transduced by lentivirus or adenovirus, and compared the hNIS expression quantitatively between the two delivery systems. MATERIALS AND METHODS: Lentiviral-mediated hNIS expressing rMSC (lenti-hNIS-rMSC) was constructed by cloning hNIS gene into pLenti6/UbC/V5-DEST (Invitrogen) to obtain pLenti-hNIS, transducing rMSC with the pLenti-hNIS, and selecting with blasticidin for 3 weeks. Recombinant adenovirus expressing hNIS gene (Rad-hNIS) was produced by homologous recombination and transduction efficiency of Rad-hNIS into rMSC evaluated by Rad-GFP was 19.1+/-4.7%, 54.0+/-6.4%, 85.7+/-8.7%, and 98.4+/-1.3% at MOI 1, 5, 20, and 100, respectively. The hNIS expressions in lenti-hNIS-rMSC or adeno-hNIS-rMSC were assessed by immunocytochemistry, western blot, and I-125 uptake. RESULTS: Immunocytochemistry and western blot analyses revealed that hNIS expressions in lenti-hNIS-rMSC were greater than those in adeno-hNIS-rMSC at MOI 20 but lower than at MOI 50. However in vitro I-125 uptake test demonstrated that iodide uptake in lenti-hNIS-rMSC (29,704+/-6,659 picomole/106 cells) was greater than that in adeno-hNIS-rMSC at MOI 100 (6,168+/-2,134 picomole/10(6) cells). CONCLUSION: Despite lower amount of expressed protein, hNIS function in rMSC was greater by lentivirus than by adenovirus mediated expression. Stem cell tracking using hNIS as a reporter gene should be conducted in consideration of relative vector efficiency for transgene expression.
Adenoviridae ; Animals ; Blotting, Western ; Clone Cells ; Cloning, Organism ; Gene Expression ; Genes, Reporter ; Homologous Recombination ; Humans ; Immunohistochemistry ; Ion Transport ; Lentivirus ; Mesenchymal Stromal Cells ; Rats ; Sodium Iodide ; Stem Cells ; Symporters ; Track and Field ; Transgenes
Adenoviridae ; Animals ; Blotting, Western ; Clone Cells ; Cloning, Organism ; Gene Expression ; Genes, Reporter ; Homologous Recombination ; Humans ; Immunohistochemistry ; Ion Transport ; Lentivirus ; Mesenchymal Stromal Cells ; Rats ; Sodium Iodide ; Stem Cells ; Symporters ; Track and Field ; Transgenes
Nuclear Medicine and Molecular Imaging.2008;42(5):383-393.
PURPOSE: Cancer specific killing can be achieved by therapeutic gene activated by cancer specific promotor. Expression of sodium iodide symporter (NIS) gene causes transportation and concentration ofiodide into the cell, therefore radioiodine treatment after NIS gene transfer to cancer cell could be a form of radionuclide gene therapy. luciferase (Luc) gene transfected cancer cell can be monitored by in vivo optical imaging after D-luciferin injection. Aims of the study are to make vector with both therapeutic NIS gene driven by AFP promoter and reporter Luc gene driven by CMV promoter, to perform hepatocellular carcinoma specific radiodiodine gene therapy by the vector, and assessment of the therapy effect by optical imaging using luciferase expression. MATERIALS AND METHODS: A Vector with AFP promoter driven NIS gene and CMV promoter driven Luc gene (AFP-NIS-CMV-Luc) was constructed. Liver cancer cell (HepG2, Huh-7) and non liver cancer cell (HCT-15) were transfected with the vector using liposome. Expression of the NIS gene at mRNA level was elucidated by RT-PCR. Radioiodide uptake, perchlorate blockade, and washout tests were performed and bioluminescence also measured by luminometer in these cells. In vitro clonogenic assay with I-131 was performed. In vivo nuclear imaging was obtained with gamma camera after I-131 intraperitoneal injection. RESULTS: A Vector with AFP-NIS-CMV-Luc was constructed and successfully transfected into HepG2, Huh-7 and HCT-15 cells. HepG2 and Huh-7 cells with AFP-NIS-CMV-Luc gene showed higher iodide uptake than non transfected cells and the higher iodide uptake was totally blocked by addition of perchlorate. HCT-15 cell did not showed any change of iodide uptake by the gene transfection. Transfected cells had higher light output than control cells. In vitro clonogenic assay, transfected HepG2 and Huh-7 cells showed lower colony count than non transfected HepG2 and Huh-7 cells, but transfected HCT-15 cell did not showed any difference than non transfected HCT-15 cell. Number of Huh-7 cells with AFP-NIS-CMV-Luc gene transfection was positively correlated with radioidine accumulation and luciferase activity. In vivo nuclear imaging with I-131 was successful in AFP-NIS-CMV-Luc gene transfected Huh-7 cell xenograft on nude mouse. CONCLUSION: A Vector with AFP promoter driven NIS and CMV promoter driven Luc gene was constructed. Transfection of the vector showed liver cancer cell specific enhancement of I-131 cytotoxicity by AFP promoter, and the effect of the radioiodine therapy can be successfully assessed by non-invasive luminescence measurement.
Animals ; Benzothiazoles ; Carcinoma, Hepatocellular ; Gamma Cameras ; Genetic Therapy ; Homicide ; Injections, Intraperitoneal ; Ion Transport ; Light ; Liposomes ; Liver ; Liver Neoplasms ; Luciferases ; Luminescence ; Mice ; Mice, Nude ; Molecular Imaging ; Optical Imaging ; Perchloric Acid ; RNA, Messenger ; Sodium ; Sodium Iodide ; Symporters ; Transfection ; Transplantation, Heterologous ; Transportation
Animals ; Benzothiazoles ; Carcinoma, Hepatocellular ; Gamma Cameras ; Genetic Therapy ; Homicide ; Injections, Intraperitoneal ; Ion Transport ; Light ; Liposomes ; Liver ; Liver Neoplasms ; Luciferases ; Luminescence ; Mice ; Mice, Nude ; Molecular Imaging ; Optical Imaging ; Perchloric Acid ; RNA, Messenger ; Sodium ; Sodium Iodide ; Symporters ; Transfection ; Transplantation, Heterologous ; Transportation
Nuclear Medicine and Molecular Imaging.2008;42(5):375-382.
A low iodine diet (LID) is the recommended preparation for radioactive iodine treatment. However, the recommended duration and stringency of LID are different among each recommendation. More stringent LID is expected in Korea because Korea is a iodine-rich region. We investigated the decrement of urine iodine excretion by two-week stringent LID for remnant thyroid ablation with radioactive iodine in Korean patients with thyroid cancer, prospectively. MATERIAL AND METHOD: From November 2006, patients who referred to our hospital for remnant ablation after total thyroidectomy were included in this study. To decrease total body iodine, our protocol included three strategies. First, we checked medication which could inhibit the radioactive iodine uptake. Second, the date of I-131 treatment was scheduled at least 3 months later if contrast agent had been used. The last strategy was two-week stringent LID education by specialized nutritionist. Before and after two-week stringent LID, 24hr-urine iodine was analyzed respectively. 24hr-urine creatinine was also analyzed for determining more valid 24hr urine sampling subgroup. RESULTS: Total 51 patients were finally enrolled. Average of 24hr-urine iodine excretion was significantly lowered (787+/-2242 -> 85+/-85 microgram/d, p=0.03) after LID and 74.4% of patients reached below the recommended urine iodine excretion level (<100 microgram/d). In subgroup (n=14), similar results was showed (505+/-666 -> 99+/-116 microgram/d, p=0.05) and 78.6% of patients met the criteria. CONCLUSION: Most patients could reach below the recommended urine iodine level after two-week stringent LID. Therefore, in our opinion, at least two-week stringent LID should be recommended in Korea.
Creatinine ; Diet ; Humans ; Iodine ; Korea ; Prospective Studies ; Thyroid Gland ; Thyroid Neoplasms ; Thyroidectomy
Creatinine ; Diet ; Humans ; Iodine ; Korea ; Prospective Studies ; Thyroid Gland ; Thyroid Neoplasms ; Thyroidectomy
Usefulness of F-18 FDG PET/CT in Staging of Peripheral T Cell Lymphoma.
Nuclear Medicine and Molecular Imaging.2008;42(5):369-374.
PURPOSE: F-18 FDG PET/CT has excellent sensitivity and specificity for staging non-Hodgkin lymphomas, but to the author's knowledge few studies to date have evaluated FDG PET/CT in peripheral T cell lymphoma. We evaluated the usefulness of F-18 FDG PET/CT in staging of patients with peripheral T cell lymphoma, especially indolent cutaneous T cell lymphomas. MATERIALS AND METHODS: Twenty five patients (M:F=17:8, age 53.7+/-14.8 yrs) with biopsy-proven indolent cutaneous T cell (CL) or noncutaneous T cell lymphomas (NCL) underwent PET/CT scans for staging at baseline. Peak standardized uptake values (p-SUV) of all abnormal foci were measured and compared between cutaneous and noncutaneous lesions. F-18 FDG PET/CT was performed on 6 patients with indolent CL and on 19 patients with NCL. RESULTS: All 6 patients with indolent CL had no significant FDG avidity in the skin despite histologically positive cutaneous lesions. However, FDG avidity appeared in extracutaneous lesions (lymph nodes) in two patients with CL where CT imaging suggested lymphoma involvement (mean p-SUV 4.26+/-0.37 in noncutaneous lesions in CL). In NCL, FDG avidity was demonstrated in all lesions where CT imaging suggested lymphoma involvement (mean p-SUV, 8.52+/-5.00 in noncutaneous lesions in NCL). CONCLUSION: F-18 FDG PET/CT has the limitation of usefulness for the evaluation of the skin in indolent CL. In contrast, F-18 FDG PET/CT is sensitive in staging evaluation of extracutaneous lesions regardless of CL or NCL.
Humans ; Lymphoma ; Lymphoma, Non-Hodgkin ; Lymphoma, T-Cell ; Lymphoma, T-Cell, Cutaneous ; Lymphoma, T-Cell, Peripheral ; Sensitivity and Specificity ; Skin
Humans ; Lymphoma ; Lymphoma, Non-Hodgkin ; Lymphoma, T-Cell ; Lymphoma, T-Cell, Cutaneous ; Lymphoma, T-Cell, Peripheral ; Sensitivity and Specificity ; Skin
Nuclear Medicine and Molecular Imaging.2008;42(5):362-368.
PURPOSE: It is well known that lung to heart ratio (LHR) is one of the high risk findings in Tl-201 myocardial perfusion SPECT. We evaluated the clinical efficacy of LHR to identify severe coronary artery disease in adenosine stress Tc-99m tetrofosmin gated myocardial perfusion SPECT (gSPECT). MATERIALS AND METHODS: The study population was 157 patients who underwent both adenosine stress Tc-99m gSPECT and coronary angiography (CAG) within one month. According to the results of CAG and gSPECT LHR and the incidence of increased LHR were compared. RESULTS: Among 53 patients with normal coronary arteries increased LHR was found in 2 patients (3.8%) and 0 in 44 patients (0%) with one vessel disease, 2 in 27 with two vessel disease (7.4%) and 8 in 33 with triple vessel disease (24.2%). Significant differences were found in LHR between subgroups of summed stress score, summed rest score and LV ejection fraction (LVEF). There were weak negative correlation between LHR and LVEF and weak positive correlation between LHR and SSS and SRS. CONCLUSION: Increased LHR had higher incidence in patients with triple vessel disease, severe LV dysfunction and/or extensive perfusion defect than those of normal group. Although its sensitivity might be low to identify severe coronary artery disease, LHR could be helpful in abnormal myocardial perfusion SPECT to stratify risk and prognosis.
Adenosine ; Coronary Angiography ; Coronary Artery Disease ; Coronary Vessels ; Glycosaminoglycans ; Heart ; Humans ; Incidence ; Lung ; Perfusion ; Tomography, Emission-Computed, Single-Photon
Adenosine ; Coronary Angiography ; Coronary Artery Disease ; Coronary Vessels ; Glycosaminoglycans ; Heart ; Humans ; Incidence ; Lung ; Perfusion ; Tomography, Emission-Computed, Single-Photon
Country
Republic of Korea
Publisher
Korean Society of Nuclear Medicine
ElectronicLinks
http://ksnm.or.krEditor-in-chief
Abbreviation
Nucl Med Mol Imaging
Vernacular Journal Title
핵의학분자영상
ISSN
1975-129X
EISSN
Year Approved
2007
Current Indexing Status
Currently Indexed
Start Year
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