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Differential Vi Haemagglutination Inhibition Test for Typhoid Carriers

Chinese Journal of Clinical Laboratory Science.1985;0(04):-.

This Paper reports the result of the differential Vi HAI test for typhoid carriers inXuzhou.One of 298 normal persons gave a false positive result.The traditional Vi HA I testfor 148 persons in the same group.6 revealed false positive.In 34 typhoid patients,thedifferential HAI test gave a titer of 1:12 to 1:64 in 16 cases(47%)Where as 10 of 239(4.1%)typhoid convalescents had the same titers.

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Isolation and Identification of Ente rotoxigenic Escherichia Coli(ETEC)

Zhilin YIN

Chinese Journal of Clinical Laboratory Science.1985;0(04):-.

The stool specimens of 235 patients with acute diarrhea were isolated and identifiedfor detection of ETEC,according to modified Elek test by purified anti-heat-labile entero-toxin serum and to the suckling mouse assay.Simultaneously,the other enteropathogenicorganisms were also isolated.33 strains of ETEC(14.04%)were obtained in total,amongwhich 29 strains(87.8%)belong to heat-stable enterotoxin(ST)type;3 strains(9.09%)heat-labile enterotoxin(LT)type and only 1 strain(3.1%)ST and LT mixed type.It wasshown that ETEC was one of the important enteric pathogens of patients with acute diar-rhea.The isolation,identification and incidence of ETEC were discussed briefly.

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A Sensitive,Rapid and Specific Assay for Double Stranded DNA Antibodies

Yuanwei LEI

Chinese Journal of Clinical Laboratory Science.1985;0(04):-.

This paper presented the method and result of enzyme-immunoassay using the CL kine-toplast as a substrate for the measurement of dsDNA antibodies.Clinical studies showedthat the results of 538 donors,984 non-rheumatic patients and 94 rheumatic diseases otherthan SLE were all negative.The positive rate of anti-dsDNA in 56 SLE sera was 55.4%.The method was compared with indirect fluorescent antibody test(CL-IF).The method isquite sensitive,simple and less expensive and can be performed by the ordinary optical mic-roscope.The micromillimetres of blood from earlobe was satisfactory.

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Evaluation of cefoxitin disk diffusion test for the detection of methicillin resistant Staphylococcus aureus

Hongwei ZHOU ; Rong ZHANG ; Gongxiang CHEN

Chinese Journal of Clinical Laboratory Science.2006;0(01):-.

Objective To establish and evaluate the method for detection of methicillin-resistant Staphylococcus aureus (MRSA) by the cefoxitin disk diffusion test.Methods The disks with 30 microgramme of cefoxitin and oxacillin recommended by NCCLS were used for the detection of MRSA with PCR by using mecA as the reference.The MIC of cefoxitin to Staphylococcus aureus was also determined.Results Of the 145 test isolates of Staphylococcus aureus,83 isolates showed the mecA-positive by PCR,62 isolates were negative.In cefoxitin disk diffusion tests,82 isolates showed MRSA-positive and 63 isolates were negative.The sensitivity was 98.8% and the specificity was 100%.In oxacillin diffusion tests,the sensitivity was 95.2% and the specificity was 96.8%.Conclusions For the detection of MRSA,the cefoxitin disk diffusion test was consistent with the results by PCR for mecA.The cefoxitin disk diffusion test is easy to be performed and can be used in routine diagnostic works.

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Correlation between expression of TIAM1 gene and carcinomas of larynx

Huihe WANG ; Guangwu HUANG ; Ling LIN ; Ligen MO ; Guoqian KUANG

Chinese Journal of Clinical Laboratory Science.2006;0(01):-.

Objective To study the relation between Tiam1 gene(T lymphoma invasion/metastasis 1)and carcinomas of larynx metastasized to lymph node.Method Using reverse transcription polymerase chain (RT-PCR) mRNA overexpression of Tiam1 gene in 30 cases of carcinoma of larynx tissue,12 lymph nodes and 10 cases of normal larynx tissue was studied.Result The frequency of TIAM1 overexpression was 75% (6/8) in primary carcinomas of larynx with metastasis but only 18.7%(4/22) in those without metastasis(P=0.0072).Overexpression of TIAM1 in metastasized lymph nodes was observed in 100% (8/8) of lymph nodes with metastasis but in only 25%(1/4) of the lymph nodes without metastasis of carcinoma(P=0.0182).The frequency of TIAM1 overexpression was 33.3% (10/30) in primary carcinomas of larynx.Conclusion Our data suggest that the overexpression of the TIAM1 gene correlates with lymph node metastasis of carcinomas of larynx.

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Analysis of tumor DNA in plasma for diagnosis of lung cancer

Xiaomei LUO ; Zhiguang TU ; Yulai WANG

Chinese Journal of Clinical Laboratory Science.2006;0(01):-.

Objective To investigate the loss of heterozygosity (LOH) of 3p D3S1300 and D3S1289 loci of plasma DNA of lung cancer (LC) patients and evaluate its value for a tumor marker of lung cancer.Methods Two microsatellite markers,D3S1300 and D3S1289,were analyzed by PCR and silver staining to investigate LOH of the plasma DNA and cancer tissue DNA from 69 cases of primary lung cancer,and the plasma DNA from 40 control subjects.Results The percentages of D3S1300 LOH detected in the tumor tissue and plasma DNA of LC patients were 40.6% and 29.0% respectively,while the percentages of D3S1289 LOH were 31.9% and 24.6% respectively.In contrast,only 2 cases of D3S1300 LOH and 3 cases of D3S1289 LOH were found in plasma DNA of control group (P

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Study on changes of protein kinase C activity in peripheral blood T lymphocytes of children with idiopathic thrombocytopenic purpura and its clinical significance

Chanlin WU ; Fang LIU ; Hong XIAO ; Qun CHEN ; Xiaomeng YANG

Chinese Journal of Clinical Laboratory Science.2006;0(01):-.

Objective To investigate the changes of protein kinase C(PKC) activity in peripheral blood T lymphocytes of the children with idiopathic thrombocytopenic purpura (ITP) and the relationships between PKC activity and T lymphocytes activation and thrombocyte decrease.Methods Sterilized peripheral blood were collected from ITP children (n=35) and healthy children (n=30).T lymphocytes were isolated and purified by the T cell segregation enrichment column.The total PKC activity was detected by non-radioactive assay.FasL,the T cell activated marker,was determined by flow cytometer.Platelet count was performed by hematocytometer.Result Compared with healthy children,total PKC activity in ITP children was significantly enhanced (0.97?0.21 nmol/min.ml vs 0.60? 0.13 nmol/min.ml,?s,P

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Frequency distributions of six short tandem repeat loci multiplexing in Jilin district,China

Bingmei WANG ; Wenyue ZHUANG ; Zhengyi LI ; Wenzhuo ZHUANG

Chinese Journal of Clinical Laboratory Science.2006;0(01):-.

Objective To analyze the genetic polymorphism of three short tandem repeat (STR) double loci,namely,six STR loci in the Chinese population of Han nationality in Jilin district and to obtain the corresponding genetic data.Methods DNA was extracted from the blood cells of one hundred and three unrelated individuals of Han nationality in Jilin district;Six loci were divided into three groups which were groupⅠ(TPOX+CSF1PO),groupⅡ(D3S1358+D13S317) and groupⅢ(D5S818+D19S400).The extracted DNA were amplified with PCR multiplexing method.The PCR products were analyzed by non-reduced PAGE,followed by silver staining.The allele frequency distributions of six STR loci were studied by statistical procedures.The heterozygosity (H),discrimination power (DP),probability of exclusion (PE) and polymorphic information content (PIC) were calculated.Results The allele frequencies of six loci were obtained.The genotypes distributions of the loci were consistent with Hardy-Weinberg equilibrium and had higher heterozygosities and PIC.The H,DP,PE and PIC were 0.7511-0.8376,0.8273-0.9247,0.5167-0.6718 and 0.7100-0.8195 respectively.Conclusions The three STR double loci exhibited higher polymorphism and were better genetic markers.The data on the allele frequencies of these six STR loci may be used in individual identification,paternity testing and in other population genetic researches.

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Studies on relationship between resistance to vancomycin and the coagulase activity of S.aureus

Hong DAI ; Xuegong FAN

Chinese Journal of Clinical Laboratory Science.2006;0(01):-.

Objective To observe the changes of coagulase activity and the DNA expression of coagulase of S.aureus during inducing vancomycin-resistant S.aureus in vitro.Methods Coagulase activity and DNA expression of coagulase of S.aureus were detected.Results The coagulase activity in 6 strains and the expression of coagulase DNA in 1 strain reduced.Conclusion Decreased coagulase activity and DNA expression are probably linked to vancomycin resistance of S.aureus.

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Detection of cyclin B1 level by RT-PCR and its significance for primary hepato cellular carcinoma

Huochun YI ; Ruxin HUANG ; Zhongying ZHANG

Chinese Journal of Clinical Laboratory Science.2006;0(01):-.

0.05).Conclusion Cyclin B1 levels in liver cancer tissues were stronger than in adjacent cancer tissues and normal liver tissues.

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