This paper presented the method and result of enzyme-immunoassay using the CL kine-toplast as a substrate for the measurement of dsDNA antibodies.Clinical studies showedthat the results of 538 donors,984 non-rheumatic patients and 94 rheumatic diseases otherthan SLE were all negative.The positive rate of anti-dsDNA in 56 SLE sera was 55.4%.The method was compared with indirect fluorescent antibody test(CL-IF).The method isquite sensitive,simple and less expensive and can be performed by the ordinary optical mic-roscope.The micromillimetres of blood from earlobe was satisfactory.