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Antimicorbial effect of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogen and effect on human gingival fibroblast cellular activity.

Tae Il KIM ; Eun Jeong CHOI ; Chong Pyoung CHUNG ; Soo Boo HAN ; Young KU

The Journal of the Korean Academy of Periodontology.2002;32(1):249-255. doi:10.5051/jkape.2002.32.1.249

Zea Mays L. has been known to be effective for improving tissue health and Magnoliae cortex to have effective antibacterial and antimicrobial activity against pathogenic microbes. The purpose of this study was to examine the antimicrobial effects of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogens(Prevotella intermedia, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans )and to examine the effects on human gingival fibroblast cellular activity. Zea Mays L. and Magnoliae cortex extracts and their mixtures were prepared with various mixing ratios (0.5:1, 1:1, 1.5:1, 2:1). These extracts were loaded to periodontal pathogen cultured petri dish for antimicrobial test and also loaded to cultured human gingival fibroblast for cellular activity test. Each test was repeated 3 times and data were analyzed by one-way ANOVA with 95% confidence level. Mixture of these two extracts showed greater amount of inhibition area on periodontal pathogen and more improved gingival fibroblast activity as Zea Mays L. ratio reduced. So, mixture ratio 0.5:1 (Zea Mays L. : Magnoliae cortex) group showed statistical significance in antimicrobial activity and cellular activity among various mixtures(p<0.05). In conclusion, 0.5:1 (Zea Mays L. : Magnoliae cortex) mixture possessed best gingival fibroblast cellular activity and antimicrobial activity toward periodontal pathogens.
Aggregatibacter actinomycetemcomitans ; Fibroblasts* ; Humans* ; Magnolia* ; Porphyromonas gingivalis ; Streptococcus mutans ; Zea mays*

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Effects of Chitosan on Human Gingival Fibroblasts in Vitro.

Ok Su KIM ; Hyun Ju CHUNG

The Journal of the Korean Academy of Periodontology.2002;32(1):235-247. doi:10.5051/jkape.2002.32.1.235

The aim of this study was to evaluate the effects of chitosan coating on the attachment, proliferation, functional and morphological change of human gingival fibroblasts. Primary culture of human gingival fibroblasts were grown in Dulbecco's modified Eagle's medium with 10% fetal bovine serum and 1% antibiotics. In experimental group, cells were inoculated in the multiwell plates coated with chitosan in concentration of 0.02, 0.2, and 2 mg/ml. Cell counting and MTT assay were done after 0.5, 1.5, 3, 6 and 24 hours of incubation to evaluate the cell attachment, and then after 2 and 7 days of culture to evaluate the cell proliferation. The alkaline phosphatase activity was measured after 4 and 7 days of culture and the ability to produce mineralized nodules was evaluated after 21 days of culture. The results were as follows : The morphology of cells on the chitosan-coated well was round or spheric. Round cells were aggregated since 6 hours of culture and showed nodule-like appearance after 24 hours of culture and did not achieved confluency at 7 days. The attachment of gingival fibroblasts was inhibited by chitosan coating with a tendency of dose dependent pattern. But, cellular activity of unit cell was higher than control. The proliferation of gingival fibroblasts was inhibited by chitosan coating at 2 mg/ml(P<0.01), while the cell proliferation at 0.02, 0.2 mg/ml was comparable to the control well. Total alkaline phosphatase activity was inhibited by chitosan coating and decreased in the course of time. While ALP activity of unit cell was the highest at 2mg/ml after 4 days of culture. Finally, gingival fibroblasts produced the mineralized nodule at 2 mg/ml. In summary, the attachment, proliferation, and alkaline phosphatase activity of gingival fibroblasts were influenced differently by the concentration of coated chitosan. From this study, it could be used as the matrix of tissue engineering for gingiva without inhibition on proliferation of gingival fibroblasts using chitosan at the optimal concentration (0.02mg/ml).
Alkaline Phosphatase ; Anti-Bacterial Agents ; Cell Count ; Cell Proliferation ; Chitosan* ; Fibroblasts* ; Gingiva ; Humans* ; Tissue Engineering

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Osteoporotic Condition in Postmenopausal Women with Periodontitis.

Kang Moon KIM ; Young Jun KIM ; Hyun Ju CHUNG

The Journal of the Korean Academy of Periodontology.2002;32(1):225-234. doi:10.5051/jkape.2002.32.1.225

The purpose of this study was to evaluate the relationship between the osteoporotic condition and periodontal condition in postmenopausal women with periodontitis. Forty three female postmenopausal patients with no systemic disease were grouped into 3 groups by their periodontal conditions; 12 mild periodontitis, 11 moderate periodontitis and 20 advanced periodontitis. From each patient, age of menopause was taken, alkaline phosphatase(ALP) and osteocalcin (OC) in blood and deoxypyridinoline (DPD) in urine were measured. Bone mineral density (BMD) of lumbar spine (L2-L4) was measured by dual energy X-ray absorptiometry. Periodontal and osteoporotic parameters were compared among the groups and correlation coefficient between them was evaluated. The blood ALP and OC levels were similar among the groups with different periodontal condition, whereas the urine DPD level and BMD were significantly lower in advanced periodontitis group than the other groups(p<0.01). Probing depth was negatively related with BMD (r=-0.5, p<0.01) and positively related with patient age and the duration of menopause (r= 0.32 and 0.35 respectively, p<0.05). Clinical attachment loss was negatively related with BMD (r=-0.66, p<0.01), and positively related with urine DPD (r= 0.37, p<0.05). These results showed that postmenopausal women with advanced periodontitis had significantly decreased bone mineral density and suggests that decreased bone mineral density in postmenopausal women could be associated with periodontal tissue breakdown.
Absorptiometry, Photon ; Bone Density ; Female ; Humans ; Menopause ; Osteocalcin ; Periodontitis* ; Spine

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Periodontal Repair on Intrabony Defects treated with BBP(R).

Hyon Su KIM ; Byeong Gap CHOI ; Seong Ho CHOI ; Kyoo Sung CHO ; Jong Jin SUH

The Journal of the Korean Academy of Periodontology.2002;32(1):213-224. doi:10.5051/jkape.2002.32.1.213

The ultimate goal of periodontal disease therapy is to promote the regeneration of lost periodontal tissue, there have been many attempts to develop a method to achieve this goal, but none of them was completely successful. The purpose of this study was to compare the effects of treatment using BBP(R) with control treated by only modified Widman flap. 22 intrabony defects from 12 patients with chronic periodontitis were used for this study, 10 sites of them were treated with BBP(R) as experimental group and 12 site were treated by only modified Widman flap as control group. Clinical parameters including probing depth, gingival recession, bone probing depth and loss of attachment were recorded at 6 months later, and the significance of the changes was statistically analyzed. The results are as follows: 1. Probing depth of control(delta2.7+/-1.3mm) and experimental group(delta3.6+/-1.8mm) weres reduced with statistically significance(P<0.05), but this changes were not different between the two experiment, control group with statistically significance. 2. Gingival recession showed statistically significant increase in control group(delta2.1+/-1.2mm)(P<0.05), but not in experimental group(delta0.5+/-0.7mm), and this changes were different between the two experiment, control group with statistically significance(P<0.05). 3. Bone probing depth showed statistically significant decrease in experimental group(delta2.9+/-1.0mm)(P<0.05), but not in control group(delta1.1+/-1.4mm), and this changes were different between the two experiment, control group with statistically significance(P<0.05). 4. Loss of attachment showed statistically significant decrease in experimental group(delta3.1+/-1.7mm), but not in control group(delta0.6+/-1.2mm), and this changes were different between the two experiment, control group with statistically significance(P<0.05) On the basis of these results, treatment using BBP(R) improves the probing depth, bone probing depth and loss of attachment in intrabony defects.
Chronic Periodontitis ; Gingival Recession ; Humans ; Periodontal Diseases ; Regeneration

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Consideration of density matching technique of the plate type direct radiologic image system and the conventional X-ray film: first step for the subtraction.

Sung Soo SO ; Hyeun Soo NOH ; Chang Sung KIM ; Seong Ho CHOI ; Kee Deog KIM ; Kyoo Sung CHO

The Journal of the Korean Academy of Periodontology.2002;32(1):199-211. doi:10.5051/jkape.2002.32.1.199

Digital substraction technique and computer-assisted densitometirc analysis detect minor change in bone density and thus increase the diagnostic accuracy. This advantage as well as high sensitivity and objectivity which precludes human bias have drawn interest in radiologic research area. The objectives of this study are to verify if Radiographic density can be recognized in linear pattern when density profile of standard periapical radiograph with the aluminium stepwedge as the reference, was investigated under varies circumstances which can be encountered in clinical situations, and in addition to that to obtain mutual relationship between the existing standard radiographic system, and future digital image systems, by confirming the corelationship between the standard radiograph and Digora system which is a digital image system currently being used. In order to make quantitative analysis of the bone tissue, digital image system which uses high resolution automatic slide scanner as an input device, and Digora system were compared and analyzed using multifunctional program, Brain3dsp. The following conclusions were obtained. 1. Under common clinical situation that is 70kVp, 0.2 sec., and focal distance 10cm, Al-Equivalent image equation was found to be Y=11.21X+46.62 r(2)=0.9898 in standard radiographic system, and Y=12.68X+74.59, r(2)=0.9528 in Digora system, and linear relation was confirmed in both the systems. 2. In standard radiographic system, when all conditions were maintained the same except for the condition of developing solution, Al-Equivalent image equation was Y=10.07X+41.64, r(2)=0.9861 which shows high corelationship. 3. When all conditions were maintained the same except for the Kilovoltage peak, linear relationship was still maintained under 60kVp, and Al-Equivalent image equation was Y=14.60X+68.86, r(2)=0.9886 in the standard radiograhic system, and Y=13.90X+80.68, r(2)=0.9238 in Digora system. 4. When all conditions were maintained the same except for the exposure time which was varied from 0.01 sec. to 0.8 sec., Al-Equivalent image equation was found to be linear in both the standard radiographic system and Digora system. The R-square was distributed from 0.9188 to 0.9900, and in general, standard radiographic system showed higher R-square than Digora system. 5. When all conditions were maintained the same except for the focal distance which was varied from 5cm to 30cm, Al-Equivalent image equation was found to be linear in both the standard radiographic system and Digora system. The R-square was distributed from 0.9463 to 0.9925, and the standard radiographic system had the tendency to show higher R-square in shorter focal distances.
Bias (Epidemiology) ; Bone and Bones ; Bone Density ; Humans ; Radiography, Dental, Digital ; X-Ray Film*

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Clinical Long-term Assessment of Bioactive Glass Graft.

Hang Bin LEE ; Chang Sung KIM ; Seong Ho CHOI ; Kyoo Sung CHO ; Chong Kwan KIM ; Choong Kyoo CHAI

The Journal of the Korean Academy of Periodontology.2002;32(1):187-198. doi:10.5051/jkape.2002.32.1.187

The ultimate goal of periodontal therapy is the regeneration of periodontal tissue which has been lost due to destructive periodontal disease. To achieve periodontal regeneration, various kinds of methods have been investigated and developed, including guided tissue regeneration and bone graft. Bone graft can be catagorized into autografts, allografts, xenografts, bone substitutes. And materials of all types have different biological activity and the capacity for periodontal regeneration, but ideal graft material has not been developed that fits all the requirement of ideal bone graft material. Recently, bioactive glass that has been utilized in plastic surgery is being investigated for application in dental practice. But, there has not been any long-term assessment of bioactive glass when used in periodontal intrabony defects. The present study evaluates the long-term effects of bioactive glass on the periodontal regeneration in intrabony defects of human and the effect of plaqu control on long term treatment results after dividing patients into those who underwent 3-month regular check-up and those who didn't under go regular check-up The clinical effect on 74sites from 17 infrabony pockets of 11 patients were analyzed 36months after treatment. 51 sites which underwent regular check up were classified as the Follow-up group(F/U group), and 23 sites which did not undergo regular check up were classified as Non Follow-up group(Non F/U group). After comparing the probing depth, attachment loss, bone probing depth before and 36months after treatment, the following results could be concluded. 1. The changes of probing pocket depth showed a statistically significant decrease between after baseline and 36 months after treatment in F/U group(1.79+/-0.68mm) and did no show astatistically significant decrease between after baseline and 36months after treatment in Non F/U group(0.61+/-0.54mm) (P<0.05). 2. The changes of loss of attachment showed a statistically significant decrease between after baseline and 36months after treatment in F/U group(1.44+/-0.74mm) and did no show astatistically significant decrease between after baseline and 36months after treatment in Non F/U group(1.18+/-1.54) (P<0.05). 3. The changes of bone probing depth showed a statistically significant decrease between after baseline and 36 months after treatment in both F/U(1.35+/-0.28) and Non F/U group(0.78+/-0.55mm) (P<0.05). The results suggest that treatment of infrabony defects with bioactive glass resulted in significan reduction of attachment loss and bone probing depth 36months after the treatment. The use of bioactive glass in infrabony defects, combined with regular check-up and proper plaque control generally shows favorable clinical results. This measn that bioactive glass could be a useful bone substitute.
Allografts ; Autografts ; Bone Substitutes ; Follow-Up Studies ; Glass* ; Guided Tissue Regeneration ; Heterografts ; Humans ; Periodontal Diseases ; Regeneration ; Surgery, Plastic ; Transplants*

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An Assessment on effect of Bioabsorbable membrane, allogenic bone and Platelet Rich Plasma in Class II furcation involvement by digital subtraction radiography.

Sang Hoon KIM ; Sung Bin LIM ; Chin Hyung CHUNG

The Journal of the Korean Academy of Periodontology.2002;32(1):173-186. doi:10.5051/jkape.2002.32.1.173

The purpose of this study was to evaluate effect of platelet rich plasma on the treatment of Grade II furcation involvement, with Demineralized Freeze-Dried Bone(Dembone(R)) and bioabsorable membrane(BioMesh(R)) in humans by digital subtraction radiography. 12 teeth(control group) were treated with Demineralized Freeze-Dried Bone(Dembone(R)) and bioabsorable membrane(BioMesh(R)), and 12 teeth(test group) were treated with Demineralized Freeze-Dried Bone(Dembone(R)), bioabsorable membrane(BioMesh(R)) and Platelet Rich Plasma. The change of bone density was assessed by digital subtraction radiography in this study. The change of mineral content by as much as 5%(vol) could be perceived in the subtracted images. The change of mineral content was assessed in the method that two radiographs are put into computer program to be overlapped and the previous image is subtracted by the later one. Both groups were statistically analyzed by Wilcoxon signed Ranks Test and Mann-whitney Test using SPSS program (5% significance level). The results were as follows: 1. In test group, the radiopacity in 3 months after surgery were significantly increased than 1 month after surgery(p<0.05). However. there were no significant difference between 1 month after surgery and 3 months after surgery in control group(p>0.05). 2. In test and control group, the radiopacity in 6 months after surgery were significantly increased than 1 month after surgery(p<0.05) 3. In test and control group, the radiopacity in 6 months after surgery were significantly increased than 3 months after surgery(p<0.05). 4. There were no significant difference between test group and control group at 1 month, 3 months after surgery, but radiopacity in test group were significantly increased than control group at 6 months after surgery(p<0.05). In conclusion, Platelet Rich Plasma can enhance bone density.
Blood Platelets* ; Bone Density ; Humans ; Membranes* ; Platelet-Rich Plasma* ; Radiography*

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Reduced alveolar bone loss in rats immunized with Porphyromonas gingivalis heat shock protein.

Ni Na YI ; Ju Youn LEE ; Jeom Il CHOI

The Journal of the Korean Academy of Periodontology.2003;33(4):555-562. doi:10.5051/jkape.2003.33.4.555

The present study has been performed to evaluate Porphyromonas gingivalis (P.gingivalis) heat shock protein(HSP)60 as a candidate vaccine to inhibit multiple bacteria-induced alveolar bone loss. Rats were immunized with P.gingivalis HSP60 and experimental alveolar bone loss was induced by infection with multiple periodonto -pathogenic bacteria. Post-immune rat anti-P.gingivalis HSP IgG levels were significantly elevated and have demonstrated highly significant inverse relationship with the amount of alveolar bone loss induced by multiple bacteria. Results from PCR detection of subgingival bacterial plaque indicated that the vaccine successfully eradicated the multiple pathogenic species. We concluded that P.gingivalis HSP60 could potentially be developed as a vaccine to inhibit periodontal disease induced by multiple pathogenic bacteria.
Alveolar Bone Loss* ; Animals ; Bacteria ; Heat-Shock Proteins* ; Hot Temperature* ; Immunoglobulin G ; Periodontal Diseases ; Polymerase Chain Reaction ; Porphyromonas gingivalis* ; Porphyromonas* ; Rats* ; Shock

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T-cell epitope specificity for Porphyromonas gingivalis heat shock protein in periodontitis.

Ji Young LEE ; Ju Yeon LEE ; Jeom Il CHOI

The Journal of the Korean Academy of Periodontology.2003;33(4):543-553. doi:10.5051/jkape.2003.33.4.543

Due to considerably high degree of sequence homology between bacterial and human heat shock proteins (hsp), it has been widely thought that this protein might be involved in autoimmune disease mechanisms in humans.To elucidate how stress proteins contribute in the immunopathogenesis of periodontitis, the present study was performed to evaluate the T cell immune responses specific to Porphyromonas gingivalis (P. gingivalis) heat shock protein (hsp)60 and T-cell epitope specificities for P. gingivalis hsp60 in periodontitis. Anti-P. gingivalis IgG antibody titers were elevated in all patients. We could establish P. gingivalis hsp-specific T cell lines from the peripheral blood of periodontitis, a mixture of CD4+ and CD8+ cells. Of 108 overlapping synthetic peptides spanning whole P. gingivalis hsp60 molecule, ten peptides with epitopes specifities for T-cell were showed. Interestingly, ten epitopes were also identified as T-cell epitopes in the present study as well as B-cell epitopes in periodontitis. Therefore, all the ten representative epitopes were designated as common T-and Bcell epitopes for periodontitis. It is critical in developing a peptide vaccine strategy for potential prevention of periodontitis. It was concluded that P. gingivalis hsp60 might be involved in the immunoregulatory process of periodontitis with heat shock protein specificities.
Autoimmune Diseases ; Cell Line ; Epitopes ; Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte* ; Heat-Shock Proteins* ; Hot Temperature* ; Humans ; Immunoglobulin G ; Peptides ; Periodontitis* ; Porphyromonas gingivalis* ; Porphyromonas* ; Sensitivity and Specificity* ; Sequence Homology ; T-Lymphocytes*

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Stem cell properties of cells derived from canine periodontal ligament.

Kyoung Hwa KIM ; Su Hwan KIM ; Yang Jo SEOL ; Yong Moo LEE

The Journal of the Korean Academy of Periodontology.2007;37(3):479-488. doi:10.5051/jkape.2007.37.3.479

In spite of the attention given to the study of mesenchymal stem cells derived periodontal ligament (PDL), there is a lack of information about canine PDL cells. In this study, we characterized canine PDL cells to clarify their stem cell properties, including self renewal, proliferate rate, stem cell markers and multipotency. PDL cells were obtained from extracted premolars of canines, following a colony forming assay and proliferation rate of sub-confluent cultures of cells for self-renewal, immunostaining for STRO-1 and CD146/MUC18 and a differentiation assay for multipotency. Canine PDL cells formed single-cells colonies and 25% of the PDL cells displayed positive staining for BrdU. The cells expressed the mesenchymal stem-cell markers, STRO-1 and CD146/MUC18. Under defined culture conditions, the cells differentiated into osteoblasts and adipocytes, but the cells didn't differentiated into chondrocytes. The findings of this study indicated that the canine PDL cells possess crucial stem cells properties, such as self-renewal and multipotency, and express the mesenchymal stem cell markers on their surface. The isolation and characterization of canine PDL cells makes it feasible to pursue preclinical models of periodontal regeneration in canine.
Adipocytes ; Bicuspid ; Bromodeoxyuridine ; Chondrocytes ; Mesenchymal Stromal Cells ; Osteoblasts ; Periodontal Ligament* ; Regeneration ; Stem Cells*

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