The Journal of the Korean Academy of Periodontology  2009;39(3):331-337

doi:10.5051/jkape.2009.39.3.331

Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans.

Yang Sin LEE 1 ; Hong Gyu PARK ; Sung Whan KIM ; Jeong Heon CHA ; Yun Jung YOO

Affiliations

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Keywords

Aggregatibacter actinomycetemcomitans; interleukin-8; periodontal ligament; reactive oxygen species

Country

Republic of Korea

Language

Korean

Abstract

PURPOSE: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells. METHODS: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS. RESULTS: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-cetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans. CONCLUSION: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.