The Journal of the Korean Academy of Periodontology  2009;39(3):359-365

doi:10.5051/jkape.2009.39.3.359

Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-1beta stimulation.

Sool Heon LEE 1 ; Ji Il PARK ; Young Joon KIM

Affiliations

+expand

Keywords

biocompatible materials; cytokines; ELISA; mineral trioxide aggregate

Country

Republic of Korea

Language

Korean

Abstract

PURPOSE: The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell. METHODS: In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-1beta on MTA was evaluated by ELISA and RT-PCR. RESULTS: The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-1beta group and MTA+IL-1beta group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-1beta group and Glass+IL-1beta group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression. CONCLUSIONS: MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.