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Screening of active components from phloroglucinols in Mianma Guanzhong for anti-neuraminidase of H1N1 influenza virus

Yantao YAN ; Juan WANG ; Donghao GENG ; Shenzhen FU ; Jinghong HU ; Zengping GAO

Journal of Beijing University of Traditional Chinese Medicine.2018;41(1):71-75. doi:10.3969/j.issn.1006-2157.2018.01.012

Objective To study the inhibitory effect of phloroglucinols in Mianma Guanzhong(Dryopteris crassirhizoma Nakai,Basket Fern Nakai)on neuraminidase(NA)of H1N1 influenza virus.Methods A library of 34 phloroglucinols ligands was established through retrieving, and X-ray three-dimensional crystal structure file of NA(H1N1)was downloaded from Protein Data Bank.The phloroglucinols possessing lower binding energy and stronger hydrogen bond interaction with NA were screened by using AutoDock Vina software and LigPlot +software, and the inhibitory effect of candidate phloroglucinols on NA was verified by using fluorometric assay.Results There were 4 phloroglucinols(filixic acids ABA, ABB, ABP and ABBA)with better combination with NA screened through molecular docking.The results of fluorometric assay showed that dryocrassin ABBA had the strongest inhibitory effect on NA [(IC50)=(26.73 ±0.57)μmol/L].Conclusion Dryocrassin ABBA in Mianma Guanzhong has higher effect of anti-NA of H1N1 influenza virus, which lays a foundation for further study on new anti-influenza drugs.

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Expression of miR-122-related factors of liver fibrosis regulated by Fuzheng Huayu Tablet

Wei ZHANG ; Mingliang SHAO ; Haicong ZHANG ; Zongyao WU ; Tongguo MIAO ; Huanwei ZHENG ; Xingtong XIE ; Rui WANG

Journal of Beijing University of Traditional Chinese Medicine.2018;41(1):76-82. doi:10.3969/j.issn.1006-2157.2018.01.013

Objective To investigate softening-hardness dissipating-binds effects of Fuzheng Huayu (reinforcing-healthy qi resolving-stasis)Tablet combined with anti-virus therapy on liver fibrosis, and relationship between these effects and regulation of pathway of miR-122-interleukin-10-reactive oxygen species(miR-122/IL-10/ROS).Methods The patients with chronic hepatitis and liver fibrosis(n=85)were chosen from Aug.2015 to Aug.2016,and then divided randomly into test group(n=45)and control group(n=40).The control group was treated with entecavir(0.5 mg/d)and monoammonium glycyrrhizinate(0.1 g/d),and test group, additionally with Fuzheng Huayu Tablet(4.5 g/d)for 48 weeks.After treatment,ISHAK fibrosis score was reviewed through routine pathology, and activities of serum superoxide dismutase(SOD)and ROS were detected by using ELISA.The expressions of miR-122 mRNA and IL-10 mRNA were detected by using RT-PCR, and content of serum inflammatory factors of liver fibrosis, including procollagen III N-terminal peptide(PCIIINP), type IV collagen(IV-C), hyaluronidase(HA)and laminin(LN),were detected by using immunoturbidimetry.Results ISHAK fibrosis score had no significant difference between 2 groups before treatment,and decreased in 2 groups after treatment,which was superior in test group to that in control group(P<0.05).The content of serum inflammatory factors of liver fibrosis all decreased in 2 groups after treatment,and the decreases of PCIIINP and HA were more significant in test group compared with control group(P <0.05).The activities of ROS and SOD had no difference in 2 groups before treatment,and ROS decreased in 2 groups after treatment.The inhibitory effect on ROS was better in test group than that in control group after treatment(P<0.05), and SOD increase was effectively relieved in test group after treatment(P <0.05).The expressions of miR-122 mRNA and IL-10 mRNA all decreased in 2 groups after treatment, which was more significant in test group compared with control group(P<0.05).Conclusion Fuzheng Huayu Tablet reduces directly the levels of collagen and HA, degrades abnormal deposition of extracellular matrix,and regulates dynamic balance of collagen,and it antagonizes effectively the genetic expressions of IL-10 and miR-122,relieves inflammatory disorders and controls progress of histology.

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Comparative study on clinical characteristics of pattern of phlegm heat and bowel excess in acute stage of stroke involving meridians-collaterals or involving zang-fu organs

Tingting CHEN ; Jinting REN ; Lina REN ; Zhongyan HE ; Chang SUN ; Donghui WANG ; Mingguang SUN ; Fang WANG ; Yingzhen XIE

Journal of Beijing University of Traditional Chinese Medicine.2018;41(1):83-88. doi:10.3969/j.issn.1006-2157.2018.01.014

Objective To explore the clinical characteristics of pattern of phlegm heat and bowel excess in acute stage of stroke involving meridian-collateral or involving zang-fu organs from the aspects of severity of neurological impairment, pattern distribution at different time points and duration of bowel excess.Methods The basic information,scores of National Institute of Health Stroke Scale(NIHSS), whether bowel qi being unobstructed or not,duration of bowel excess,and pattern distribution at different time points(onset for 1 d to 3 d,onset for 4 d to 7 d and onset for 8 d to 14 d)were inputted based on structured data by using retrospective analysis of medical records in 204 cases of pattern of phlegm heat and bowel excess of stroke involving meridian-collateral(meridian-collateral group)and 217 cases of pattern of phlegm heat and bowel excess of stroke involving zang-fu organs(zang-fu organ group).The clinical characteristics from the data were compared, analyzed and studied.Results The severity of neurological impairment except of conscious state was significantly higher in zang-fu organ group than that in meridian-collateral group(P<0.01).The average duration of unobstructed bowel qi was significantly longer in zang-fu organ group than that in meridian-collateral group(P<0.01), and the percentage of patients with throughout obstructed bowel qi was significantly higher in zang -fu organ group than that in meridian-collateral group in acute stage of stroke.The pattern of phlegm heat and bowel excess showed a decline trend after stroke onset for 1 d to 14 d in meridian-collateral group, and showed an ascending trend after stroke onset for 1 d to 7 d and a decling trend after stroke onset for 8 d to 14 d in zang-fu organ group.The cases of pattern of qi deficiency with blood stasis or pattern of collaterals blocked by wind -phlegm and static blood were significantly higher in meridian-collateral group at 4-14 d than those at 1-3 d,and cases of pattern of wind stirring due to yin deficiency,pattern of phlegm heat and blood stasis or pattern of wind phlegm and blood stasis were significantly higher in zang-fu organ group at 8 -14 d than those at time point 1.Conclusion The duration of pattern of phlegm heat and bowel excess is posi-tively correlated to the severity of stroke,that is,neurological impairment is more serious and duration is longer in patients with pattern of phlegm heat and bowel excess of stroke involving zang -fu organs.

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Medication rules of anti-tumor formulas containing marine Chinese medicinals

Mingchan WEI ; Jiang LIN ; Jincang YI ; Yuanming TONG ; Diya ZHANG ; Tianjun ZOU

Journal of Beijing University of Traditional Chinese Medicine.2018;41(3):253-258. doi:10.3969/j.issn.1006-2157.2018.03.013

Objective To investigate the rules of medication and combinations of anti-tumor formulas containing marine Chinese medicinals. Methods The anti-tumor formulas containing marine Chinese medicinal were collected from Marine Chinese Medicinal and Effective formulas, Dictionary of Chinese Marine Medicinals, Marine Chinese Materia Medica, and databases including CNKI. A database was established for data mining by using V2. 5 software of TCM inheritance support system (TCMISS). Results There were totally 251 anti-tumor formulas containing marine Chinese medicinals involved 417 Chinese medicinal including 36 marine Chinese medicinals. The nature of medicinals in these formulas were mainly cold, neutral and warm, flavors of them were mainly bitterness, pungency, sweetness and saltiness,and they mainly entered into meridians of liver,lung,stomach,spleen and heart. The analysis of association rules showed that core combination was Muli (Oyster Shell, Concha Ostreae)-Haizao (Seaweed, Sargassum)-Kunbu (Kelp, Thallus Laminariae), which was commonly combined with medicinals with effects of clearing heart,tonifying,resolving phlegm,dissipating binds,activating blood and resolving stasis in clinical practice. Entropy clustering analysis showed that there were 12 combinations with core medicinals,and they were Haizao-Haigeqiao(Clam Shell,Goncha Meretricis seu Cyclinae)-Kunbu, Zaojiaoci (Chinese Honeylocust Spine, Spina Gleditsiae)-Chuanshanjia (Pangolin Scales,Squama Manitis)-Jiangcan (Batryticated Silkworm, Bombyx Batryticatus), Muxiang (Common Aucklandia Root,Radix Aucklandiae)-Walengzi (Ark Shell, Concha Arcae)-Puhuang (Cattail Pollen, Pollen Typhae), Chansu (Toad Venom, Venenum Bufonis)-Xionghuang (Realgar, Realgar)-Zhenzhu (Pearl,Pernulo),Huangbai(Amur Cork-Tree,Cortex Phellodendri)-Yunaoshi(Fish Otolith,Asteriscus Pseudosciaenae)-Bingpian (Bomeol, Bomeolum Syntheticum), Haima (Sea Horse, Hippocampus)-Qingpi (Green Tangerine Peel, Pericarpium Citri Reticulatae Viride)-Qianniuzi (Pharbitis Seed, Semen Pharbitidis), Haizao-Kunbu-Gancao (Liquorice Root, Radix Glycyrrhizae), Wangbuliuxing (Vaccaria Seed,Semen Vaccariae)-Chuanshanjia-Muli, Fengfang (Hornet Nest, Nidus Vespae)-Walengzi-Qianxie (Scorpion,Scorpio),Shexiang (Musk,Moschus)-Xuejie (Dragon's Blood,Risina Draconis)-Zhenzhu, Huangbai (Amur Cork-Tree, Cortex Phellodendri)-Ruxiang (Frankincense, Olibanum)-Bingpian, and Qingpi-Wulingzhi (Trogopterus Dung, Faeces Trogopterorum)-Dafupi (Shell of Arecanut, Pericarpium Arecae). There were 6 new combinations, and they were Haizao-Haigeqiao-Kunbu-Gancao, Zaojiaoci-Chuanshanjia-Jiangcan-Wangbuliuxing-Muli, Muxiang-Walengzi-Puhuang-Fengfang-Qianxie, Chansu-Xionghuang-Zhenzhu-Shexiang-Xuejie, Huangbai-Yunaoshi-Bingpian-Ruxiang, and Haima-Qingpi-Qianniuzi-Wulingzhi-Dafupi. The high-frequency tumor diseases, totally 47, treated with anti-tumor for-mulas containing marine Chinese medicinals,were successively thyroid tumor,lung cancer,liver cancer, uterine fibroids, nasopharyngeal carcinoma, esophageal cancer, gastric cancer and mammary cancer. Conclusion There are rules to follow in treatment of tumors with marine Chinese medicinal. The different therapeutic effects can be achieved by different medicinal combinations,which has a very important value in guiding clinical medication and new medicinal development.

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Treatment of stenosal tendosynovitis with percutaneous micro-invasive release therapy by using push-cut knife: a clinical trial

Changhe YU ; Tao LUO ; Changxin LIU ; Yujie ZHAO ; Guihua TIAN ; Xiyou WANG ; Jing LIU ; Fu WANG

Journal of Beijing University of Traditional Chinese Medicine.2018;41(3):259-264. doi:10.3969/j.issn.1006-2157.2018.03.014

Objective To evaluate the effectiveness and safety of percutaneous micro-invasive release therapy by using push-cut knife on stenosal tendosynovitis. Methods The patients with stenosal tendosy-novitis were chosen according to inclusion criteria,and then divided,based on random digital table and 1:1 of random concealmert,into push-cut knife group and needle-knife group. The push-cut knife group was given push-cut knife therapy one time, and was given traditional needle-knife one time. The main outcome index was cure rate on the 27thweek after treatment, and secondary indexes included pain scores,joint range of motion (ROM) and trigger indications. The safety indexes included complications and adverse events. Results There were totally 120 patients were chosen,but one quitted due to serious disease in needle-knife group,and 119 patients completed the study and were brought into the statistical analysis of outcome indexes. On the 27thweek after treatment,there were 55 cases(91.67%) cured in push-cut knife group and 39 (66.10%) cured in needle-knife group (P<0.001). The indexes of trig-ger indications,pain and ROM were significantly relieved in 2 groups,and improved more significantly o-ver time. The comparisons at the time points of the 1st,3rd,6th,12thand 27thweeks showed that,the re-liefs of trigger indications, pain and ROM were superior in push-cut knife group than those in needle-knife group (P<0.05). There were no adverse events or complications occurred in 2 groups. Conclu-sion The push-cut knife has higher one-off cure rate in treatment of stenosal tendosynovitis. The signs of pain,ROM and trigger indications are relieved immediately after treatment,and continuously relieved af-ter half year. There are no adverse events occurred.

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Quantitative determination of phenolic characteristic components and their quality representation and correlation analysis of specific chromato-grams in Shaji

Yuanyuan ZHANG ; Shan LYU ; Mengjie CHEN ; Jing KONG ; Rendi SUN ; Yuanyuan ZHANG ; Dong-Bo LU ; Qiuhong WU ; Wenxia LI ; Menglin WANG ; Yuanyuan FAN ; Xin FENG ; Sitong LI ; Yu-Juan HUANG ; Yanyan JIANG ; Renbing SHI

Journal of Beijing University of Traditional Chinese Medicine.2018;41(5):383-394. doi:10.3969/j.issn.1006-2157.2018.05.006

Objective To establish an analysis method for quantitatively determining phenolic character-istic components and correlation analyzing their quality representation of specific chromatograms in Shaji (Seabuckthorn Fruit,Hippophae rhamnoides),and to review the quality of Shaji effectively and accurate-ly by applying association analysis-reviewing mode.Methods HPLC-PDA method was used to quantita-tively determine the content of phenolic characteristic components in 11 batches of Shaji(protocatechuic acid,ellagic acid,narcissin,quercetin,isorhamnetin),and to establish phenolic specific chromatograms of Shaji.The quality of 11 batches of Shaji was characterized based on teasing characteristic peaks and chemical types.The quantity of 11 batches of Shaji was characterized based on quantity and peak areas of protocatechuic acid, ellagic acid, narcissin, quercetin, isorhamnetin, phenolic acids(represented by protocatechuic acid)and flavonoids(represented by narcissin)in the specific chromatograms.The characterized results of quality and quantity of 11 batches of Shaji were given association analysis based on baseline material of Shaji.Results The characteristic components of protocatechuic acid, ellagic acid,narcissin,quercetin,isorhamnetin all had good linear correlation,and the results of methodological investigation were in accordance with the quantitative determination requirements.Taken batch 7 of Shaji as baseline material, there were totally 18 characteristic peaks in phenolic specific chromatograms of Shaji,including 3 peaks of phenolic acids and 15 peaks of flavonoids, and all 18 characteristic peaks appeared in the chromatograms of 11 batches of Shaji.The quantity of characteristic components were higher in batch 8, 7, 10, 4, 5, 11 and 1 after analyzed and reviewed by using association analysis-reviewing mode.The relevance of batch 6, 2, 3, 1 and 11 was the highest with baseline material of Shaji.Comprehensive reviewing showed that the excellent extents of batch 1,11,8,6 and 7 were prior. Conclusion The quantitative determination method of phenolic characteristic components in Shaji estab-lished in this study is easy and accurate.The association analysis-reviewing mode for quality characteriza-tion of phenolic specific chromatograms can be used for analyzing the quality and application validity of Shaji and reviewing quality of Shaji effectively and accurately.

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Content and influencing factors of tobeimoside I in Tubeimu from different producing areas analyzed by using group-average clustering method

Ning TAN ; Yang LU ; Yutao XUE ; Yanping LI ; Yanling ZENG ; Shouying DU

Journal of Beijing University of Traditional Chinese Medicine.2018;41(5):395-399. doi:10.3969/j.issn.1006-2157.2018.05.007

Objective To identify the content of tubeimoside I in Paniculate Bolbostemma from 16 differ-ent locations and determine the high-containing locations and influencing factors.Methods The quanti-tative determination method of tobeimoside I in Tubeimu(Paniculate Bolbostemma, Rhizoma Bolbostem-atis)in Chinese Pharmacopoeia(2015 Edition)was used to determine the content of tobeimoside I in 16 batches of Tubeimu for deciding producing areas with higher content of Tubeimu and analyzing the influ-encing factors.The origin producing areas was classified by using group-average clustering analysis meth-od taken content of tobeimoside I as the index.Results The results showed that the content of tobeimo-side I was in a range from 1.234%to 3.174%in different producing areas,which was accorded with the requirement of Chinese Pharmacopoeia.The clustering analysis classified 16 producing areas into 4 groups,and content of tobeimoside I was the highest in Tubeimu material produced from Shangluo and Hanzhong.Conclusion The temperature and humidity had influence on Tubeimu material, and content of tobeimoside I was higher in the producing areas with higher temperature and humidity.

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Quantitative determination of N-caffeoylputrescine, kukoamine B and chlorogenic acid in Digupi

Lei SU ; Yanyan JIANG ; Luxiao CHEN ; Jing LIU ; Bin LIU

Journal of Beijing University of Traditional Chinese Medicine.2018;41(5):400-404. doi:10.3969/j.issn.1006-2157.2018.05.008

Objective To establish a method for quantitative determination of N-caffeoylputrescine, kukoamine B and chlorogenic acid in Digupi(Chinese Wolfberry Root-bark,Cortex Lycii).Methods The preparation method for solution of test sample was established by using single-factor investigation. HPLC procedure was performed for determining the content of N-caffeoylputrescine, kukoamine B and chlorogenic acid on chromatographic column of Waters Xbridge C 18(4.6 mm×250 mm,5 μm),mobile phase was methanol -0.05%trifluoroacetic acid in gradient elution.The flow velocity was 1.0 mL/min and the detection wavelength was 290 nm at 30℃.Results The solution of test sample was prepared af-ter Digupi was extracted with 100 times amount of 70% methanol by using reflux extraction for 60 min. N-caffeoylputrescine showed a good linear relationship at a range from 0.013 75 mg/mL to 0.220 0 g/L (r=0.999 5),and average recovery was 99.25%(RSD=1.81%, n=6).Kukoamine B showed a good linear relationship at a range from 0.057 875 g/L to 0.926 0 mg/mL(r=0.999 8), and average recovery was 101.01%(RSD=2.81%,n=6).Chlorogenic acid showed a good linear relationship at a range from 0.009 25 g/L to 0.148 0 g/L(r=0.999 5), and average recovery was 99.51%(RSD=2.32%,n=6).Conclusion The established quantitative determination method is easy and accurate with a good repeatability,which can lay a foundation for the quality control and reviewing of Digupi.

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Expressing patterns of serum inflammatory factors in patients with gas-tric cancer with different types of tongue fur

Junfeng ZHANG ; Liwei ZHANG ; Juan WU ; Qianqian XIN ; Wei DONG ; Ruiping WANG ; Zhen ZHAN

Journal of Beijing University of Traditional Chinese Medicine.2018;41(5):405-412. doi:10.3969/j.issn.1006-2157.2018.05.009

Objective To investigate the expressing patterns of serum inflammatory factors in patients with gastric cancer(GC)with different types of tongue fur.Methods GC patients with different types of longue fur(n=134)and non-GC subjects with different types of tongue fur(n=93)were chosen from clinic.The levels of serum inflammatory factors were detected by using electro-chemiluminescence immu-noassay(ECLIA),and correlation between types of tongue fur and serum inflammatory factors was ana-lyzed by using multi-factor association method.Results The levels of serum interleukin-17α(IL-17α) had significant difference in GC patients with different types of longue fur(P<0.05).The analysis on fur color showed that level of serum IL-17αwas 3.062 ng/L in GC patients with yellow fur and 3.982 ng/L in those with white fur(P<0.05).ROC analysis showed that the sensitivity was 72.7%,specific-ity was 91.3%and AUC(95% CI)was 0.639(0.539 -0.739)of serum IL-17αin distinguishing white fur and yellow fur,and cutoff value was 6.033 ng/L.The similar tendency was identified in non-GC subjects.The correlation analysis showed that there were 20 serum inflammatory factors with signifi-cant positive correlation in GC patients with the same types of tongue fur,and significant negative correla-tion in GC patients with different types of tongue fur.Conclusion The level of IL-17αcan significantly distinguish white tongue fur and yellow tongue fur.The white-yellow tongue fur and thin-thick tongue fur may reflect adverse inflammatory status in GC patients.

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Time series analysis on incidence trend of dysentery in Shanxi Province

Ling HAN ; Long YAN ; Yu HAO ; Keerqinfu ; Hong WANG ; Juan HE

Journal of Beijing University of Traditional Chinese Medicine.2018;41(5):413-417. doi:10.3969/j.issn.1006-2157.2018.05.010

Objective To investigate the feasibility and applicability of model of seasonal autoregressive integrated moving average(SARIMA)in analysis and prediction on dysentery morbidity in Shanxi Prov-ince,and to provide decision basis for preventing and controlling dysentery.Methods SARIMA model was established based on monthly morbidity of dysentery from Jan.2004 to Dec.2013 by using R3.3.1 software,and the predictive effect of the model was verified with monthly morbidity of dysentery in 2014. Results SARIMA model(2,1,2)(1,1,1)12fitted very well with the monthly dysentery morbidity in Shanxi Province,and the residuals of the model were white noise sequence.The relative error between predicted and actual values at a range from 0.909% to 35.575% and average relative error was 13.399%.Conclusion SARIMA model can well reflect the incidence trend of dysentery and predict the morbidity of dysentery in a short term in Shanxi Province.

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