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Experience of the support of medicine in Harmonious Mission -2018

Xiaoguang LI ; Panpan ZHANG ; Jinghong XU ; Yunxia WANG ; Songwei LU ; Min LIU ; Shen GAO

Journal of Pharmaceutical Practice.2020;38(1):1-4. doi:10.3969/j.issn.1006-0111.201903091

Objective Through the statistics of drug consumption in the task of Harmonious Mission -2018, sort out the process of medication supply of overseas medical services, analyze and evaluate the medication supply services. Methods Count the types and quantities of medicines consumed by marine hospitals in the Harmonious Mission-2018, analyze whether the preparation of medicines is sufficient, effective, and economically reasonable, and find way to improve the process of medicine supply in overseas medical services. Results Statistics on the consumption of drugs in the Harmonious Mission-2018 show that the number of drugs consumed accounted for 68.21% of the total kinds of medicine carried, that the total amount of drugs consumed accounted for 40.61% of the total costs of medicine carried, and that the total number of boxes of drugs consumed (the number of the smallest packages) accounted for 21.72% of the total number of boxes of medicine carried. Conclusion The medicine support services in the Harmonious Mission-2018 was sufficient and effective. But, the workflow and the processes of the services still need to be further improved.

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Analysis of drug consumption in medical service for Dominica and Dominican in Harmonious Mission -2018

Panpan ZHANG ; Min LIU ; Jinghong XU ; Yunxia WANG ; Xia TAO

Journal of Pharmaceutical Practice.2020;38(1):5-8. doi:10.3969/j.issn.1006-0111.201908043

Objective To analyze the drug consumption in medical service for Dominica and Dominican in Harmonious Mission -2018, and provide reference for future drug supply services in similar countries. Methods Data of the drugs used during the medical service of the two countries were extracted from the computer system, which were summarized and analyzed. Results Ranking first in drug consumption in medical service for Dominica and Dominican was external drugs. Chinese traditional medicines were very popular in both two countries. There were great similarities and unique characteristics in the drug consumption during medical service between the two countries, orthopedic drugs, digestive system drugs, ophthalmic drugs and dermatological drugs were frequently used drugs. Conclusion The drug consumption in medical service was closely associated with the national conditions. Therefore, in order to prepare medicines reasonably in future overseas medical service, disease distribution of the country which would be visited and drug consumption in the similar country which had been visited before should be concerned.

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The role and mechanism of inflammasome-associated pyroptosis in nonalcoholic fatty liver disease

Wenjie ZHANG ; Diyang SUN ; Pei WANG

Journal of Pharmaceutical Practice.2020;38(1):9-13. doi:10.3969/j.issn.1006-0111.201902051

Non-alcoholic fatty liver disease (NAFLD), which is a series of diseases including simple fatty liver, nonalcoholic steatohepatitis and cirrhosis, is one of the main causes of liver cirrhosis, carcinoma and liver transplantation. The mechanism of NAFLD is not fully understood. Besides the physical exercise and diet intervention, there is no recognized effective drug therapy for NAFLD. Pyroptosis is a newly discovered type of programmed cell death, which is associated with activation of caspase-1, or caspase-2 mediated inflammasome activation. The main function of inflammasome is to activate caspase-1, which indirectly regulates the expression and secretion of inflammatory factors interleukin-1 and interleukin-18. Recent studies have shown that inflammasome-associated pyroptosis plays a key role in NAFLD. This review will discuss the latest researches in this field to provide new scientific knowledge for prevention and treatment of NAFLD.

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Research progress of valproic acid genomics

Xun ZHOU ; Luojiao TAN ; Hongyu ZHAO ; Shouhong GAO

Journal of Pharmaceutical Practice.2020;38(1):14-17. doi:10.3969/j.issn.1006-0111.201906018

Valproic acid is a commonly used and broad-spectrum antiepileptic drug in clinical practice with a narrow therapeutic window. Valproic acid has a great individual difference in its metabolism which is influenced by many factors. The gene polymorphism of drug metabolic enzymes is one of the critical factors. Through consulting relevant articles, the affection of genomics and clinical treatment on valproic acid clinical application were reviewed in this paper, which provided a reference for clinical individualized treatment.

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Research on the improving function of endothelial progenitor cells in diabetes

Jingxue LIU ; Jin HUANG

Journal of Pharmaceutical Practice.2020;38(1):18-21. doi:10.3969/j.issn.1006-0111.201906065

Endothelial progenitor cells (EPC) are stem cells derived from bone marrow and peripheral blood, which could proliferate and differentiate into endothelial cells and participate in repair after tube injury. Studies have shown that the improving function of diabetic EPC could prevent and reduce the occurrence of diabetic complications. The research progress in improving the function of EPC in recent years and the improvement of function in the development of diabetes were reviewed in order to provide new ideas for the treatment of diabetes.

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Comparisons and current situation analysis of drug purchase modes in military hospitals

Ming SUN

Journal of Pharmaceutical Practice.2020;38(1):22-26. doi:10.3969/j.issn.1006-0111.201908097

During the new round of medical system and military system reform, the current drug procurement mode in military hospitals dose not keep up with the development of national drug procurement policy. This article summarized the current situation of medication procurement in military hospitals through the study of national drug procurement policies for domestic public hospitals and foreign public medical institutions. The new drug procurement mode for military hospitals was discussed in order to lay the foundation for the Civilian-military Integration of military hospitals.

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Optimization of the compound herba lysimachiae extraction prosess based on orthogonal experiment and response surface method

Pengchao PAN ; Xiaofei CHEN ; Wei CHEN ; Zhiyong GUO ; Yifeng CHAI

Journal of Pharmaceutical Practice.2020;38(1):27-34. doi:10.3969/j.issn.1006-0111.201910001

Objective To optimize the extraction process of the compound herba lysimachiae by orthogonal experiment and response surface method. Methods The sum of the peak areas of multiple representative components in the compound herba lysimachiae was used as the evaluation index. The ultrasonic extraction time, ultrasonic temperature and ethanol ratio were analyzed to optimize the extraction process conditions by orthogonal experiment and Box-Behnken response surface analysis method. Results Ultrasonic extraction time and ethanol ratio are important factors in the extraction process of the compound herba lysimachiae. The effect of ultrasonic temperature in the extraction process is not significant. The optimal extraction condition for compound herba lysimachiaeare is 10 min ultrasonic extraction time at 55 ℃ with 55% ethanol. Conclusion The optimal extraction process parameters obtained by combining orthogonal experiment and Box-Behnken response surface method can be used for the extraction of representative components from the compound herba lysimachiae and pharmaceutical research in the future.

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Design, synthesis and biological activity of non-nucleoside NEDD8-activating enzyme inhibitors

Chuan LUO ; Zhiliang YU ; Wannian ZHANG ; Zhenyuan MIAO

Journal of Pharmaceutical Practice.2020;38(1):35-41. doi:10.3969/j.issn.1006-0111.201901012

Objective To develop novel NAE inhibitors with non-nucleoside scaffold by a scaffold hopping strategy and study the in vitro antitumor activities. Methods Disulfonamideindazole 14 was synthesized through 23 steps with a good yield. Its chemical structure was confirmed by ¹H NMR and MS. MTT method was used to determine the in vitro antitumor activities. Results Compound 14 exhibited moderate antitumor activities against various cancer cells and promoted significant UBC12 accumulation in a dose-dependent manner. Conclusion Compound 14 is a potent NAE inhibitor with remarkable apoptosis induction and cell cycle arrest in prostate cancer PANC-1 cells. Our work provides a valuable leading compound for the further design and development of NAE inhibitors.

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Establish and optimization of inter-simple sequence repeat PCR reaction system of Gnaphalium affine

Shenggui JIANG ; Zhiying DONG ; Qing LI ; Yingkui ZHAO ; Doudou HUANG ; Lianna SUN

Journal of Pharmaceutical Practice.2020;38(1):42-46. doi:10.3969/j.issn.1006-0111.201909048

Objective To provide the experimental basis for the subsequent genetic diversity research through establishing and optimizing the inter-simple sequence repeat PCR (ISSR-PCR) reaction system of Gnaphalium affine. Methods The single-factor experimental method and full experimental method were used to optimize the ISSR-PCR reaction system of Gnaphalium affine. Under the optimal system, after screening primers and corresponding annealing temperatures, the systematic feasibility was verified. Results The optimal ISSR-PCR reaction system was consisted of 10 μl Premix Taq DNA polymerase, 0.3 μmol/L primer, 10 ng DNA template, and sterilized water added to 20 μl. Finally, 10 primers were screened from 100 universal primers, and verification results indicated the system had high stability, good reproducibility, and the selected primers had good polymorphism. Conclusion The ISSR-PCR amplification system of Gnaphalium affine was established for the first time and the primers with appropriate annealing temperatures were filtered out, which provided a reference for the subsequent genetic diversity research of Gnaphalium affine.

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The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer

Jing ZHANG ; Yongwei GU ; Xin WU

Journal of Pharmaceutical Practice.2020;38(1):47-51. doi:10.3969/j.issn.1006-0111.201906038

Objective To synthesize a novel prostate cancer targeting gene vector PAMAM-PEG-C2min and improve gene transfection efficiency targeting on prostate cancer. Methods The aptamer (C2min) and polyamide-amine (PAMAM) were ligated by polyethylene glycol (PEG). The structure of the synthesized PAMAM-PEG-C2min was identified by NMR. The biological characteristics of the nanoparticles were examined by the uptake experiments and gene transfection experiments (the loaded gene was siR-M) with the prostate cancer cells (PC3 and LNCaP). Besides, the in vivo targeting was investigated using in vivo image system. The in vivo targeting results indicated that PAMAM-PEG-C2min can achieve the simultaneous targeting of two prostate cancer tissues. Results The PAMAM-PEG-C2min synthesis was confirmed by NMR. Cell uptake experiments showed that the cell uptake efficiency of PAMAM-PEG-C2min was concentration dependent. In vitro experiments showed that the PC3 and LNCaP cells transfection efficiency and targeting of PAMAM-PEG modified with C2min were significantly improved compared with the PEG modified PAMAM. Conclusion PAMAM-PEG-C2min is a potential targeted drug delivery vehicle. It provides a new technology platform for comprehensive and specific targeting treatment of prostate cancer.

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