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The Possible Role of Mesenchymal Stem Cells Therapy in the Repair of Experimentally Induced Colitis in Male Albino Rats.

Sohair Ahmed FAWZY ; Rahma Kamal El din ABO-ELNOU ; Dalia Fathy Abd El Maksoud EL-DEEB ; Marwa Mohamed Yousry ABD-ELKADER

International Journal of Stem Cells.2013;6(2):92-103.

BACKGROUND AND OBJECTIVES: Colitis is inflammation of the colon which can be transmural or confined to the mucosa. Colitis may be acute or chronic. In case of serious intestinal discontinuity of epithelium, the regeneration capacity of local stem cells is not enough to complete tissue repair. Bone marrow mesenchymal stem cells (BM-MSCs) migrate into the gastrointestinal wall, where they may contribute to the repair progress. The present study aimed at evaluating the possible therapeutic effect of MSCs on induced colitis in albino rat. METHODS AND RESULTS: Twenty male albino rats were divided into 3 groups (control, colitis, MSCs), control group (4 rats), colitis group (8 rats) received once intra-rectal injection of 2 ml of 3% acetic acid. MSCs therapy group (8 rats) injected with MSCs 24 hours after colitis induction. In each group, rats were subdivided into subgroups (a & b). Subgroup (a) corresponds to rats sacrificed 3 days and subgroup (b) corresponds to rats sacrificed 10 days after colitis induction. Isolation and culture of MSCs from rat bone marrow were performed. Colon sections were examined using light and fluorescent microscopy. Colon specimens were subjected to histological, morphometric and statistical studies. In colitis group, ulceration, loss of surface columnar epithelium, disturbed crypts architecture with few goblet cells and huge lymphatic nodule piercing the muscularis mucosa were reported. In stem cell therapy group, MSCs stimulate colonic repair through differentiation into several cells and dampen the inflammation. CONCLUSIONS: MSCs represent future therapeutic hopes for intestinal injury and chronic intestinal inflammatory states.
Acetic Acid ; Animals ; Bone Marrow ; Colitis* ; Colon ; Epithelium ; Goblet Cells ; Humans ; Inflammation ; Male* ; Mesenchymal Stromal Cells* ; Microscopy ; Mucous Membrane ; Organic Chemicals ; Rats* ; Regeneration ; Statistics as Topic ; Stem Cells ; Ulcer

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Biomimetic Polymer Scaffolds to Promote Stem Cell-Mediated Osteogenesis.

Eunkyung KO ; Seung Woo CHO

International Journal of Stem Cells.2013;6(2):87-91.

Bone tissue engineering using stem cells with osteogenic potential is a promising avenue of research for bone defect reconstruction. Organic, inorganic, and composite scaffolds have all been engineered to provide biomimetic microenvironments for stem cells. These scaffolds are designed to promote stem cell osteogenesis. Here, we review current technologies for developing biomimetic, osteoinductive scaffolds for stem cell applications. We summarize the reported in vitro and in vivo osteogenic effects of these scaffolds on stem cells.
Biomimetics* ; Bone and Bones ; Osteogenesis* ; Polymers* ; Stem Cells

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Specific Protein Markers for Stem Cell Cross-Talk with Neighboring Cells in the Environment.

Kyung Soo PARK ; Seung Won SHIN ; Jeong Woo CHOI ; Soong Ho UM

International Journal of Stem Cells.2013;6(2):75-86.

A stem cell interacts with the neighboring cells in its environment. To maintain a living organism's metabolism, either cell-cell or cell-environment interactions may be significant. Usually, these cells communicate with each other through biological signaling by interactive behaviors of primary proteins or complementary chemicals. The signaling intermediates offer the stem cell's functionality on its metabolism. With the rapid advent of omics technologies, various specific markers by which stem cells cooperate with their surroundings have been discovered and established. In this article, we review several stem cell markers used to communicate with either cancer or immune cells in the human body.
Human Body ; Metabolism ; Stem Cells*

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An Analysis of Blood Utilization for Stem Cell Transplant Patients in a Tertiary Care Hospital.

Natasha ALI

International Journal of Stem Cells.2017;10(1):114-118. doi:10.15283/ijsc16045

BACKGROUND AND OBJECTIVE: Haematopoietic stem cell transplant is a potentially curative treatment option in various benign and malignant haematological diseases. Patients undergoing stem cell transplant procedure require blood transfusion on a daily basis. Currently, there is paucity of data from developing countries on transfusion practices. This audit was undertaken to determine the consumption of packed red blood cells (PRBCs) transfusion in the bone marrow transplant unit of the Aga Khan University Hospital. SUBJECTS AND METHODS: A retrospective audit was conducted for packed red cell transfusion ordering practice over a period from June 2014~June 2015. All consecutive patients, admitted for stem cell transplant procedure for various underlying diseases were included. Outcome measures used in this study were (i) cross match to transfusion (C: T) ratio and (ii) transfusion trigger. RESULTS: During the study period, n=25 patients underwent haematopoietic stem cell transplant. There were n=19 males and n=6 females. One patient was less than 15 years of age while rests were adults. Median age±SD was 26.5±14.5 years (12~54 years). The underlying diagnosis included Aplastic anemia (n=8), Thalassemia major (n=3), Multiple Myeloma (n=4), Acute leukemia (n=5), Hodgkin’s lymphoma (n=4), PRCA (n=1). Grand total consumption of PRBCs during the study period was 204 while 258 products were crossmatch. The C:T ratio was 1.26. The transfusion trigger was Hb level of less than 8 gms/dl. CONCLUSION: The results of our BMT unit indicate that the C:T ratio and transfusion trigger is comparable to the international benchmark.
Adult ; Anemia, Aplastic ; Benchmarking ; beta-Thalassemia ; Blood Transfusion ; Bone Marrow ; Developing Countries ; Diagnosis ; Erythrocytes ; Female ; Humans ; Leukemia ; Lymphoma ; Male ; Multiple Myeloma ; Outcome Assessment (Health Care) ; Retrospective Studies ; Stem Cells* ; Tertiary Healthcare*

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Cavin-2 Functions as a Suppressive Regulator in TNF-induced Mesenchymal Stromal Cell Inflammation and Angiogenic Phenotypes.

Bayader ANNABI ; Alain ZGHEIB ; Borhane ANNABI

International Journal of Stem Cells.2017;10(1):103-113. doi:10.15283/ijsc16032

Tumour necrosis factor (TNF)-α activation of mesenchymal stromal cells (MSC) enhances their tumour-suppressive properties and tumour-homing ability. The molecular actors involved are unknown. We found that TNF induced MSC migration and tubulogenesis which correlated with a dose-dependent increase in Cavin-1 and Cavin-3 transcript levels. TNF triggered cyclooxygenase (COX)-2 expression, whereas specific siRNA-mediated gene silencing of Cavin-2 resulted in an amplified COX-2 expression, tubulogenesis, and migratory response partially due to a rapid and sustained increase in NF-κB phosphorylation status. Our results highlight a suppressive role for the caveolar component Cavin-2 in the angiogenic and inflammatory regulation of TNF-activated MSC.
Gene Silencing ; Inflammation* ; Mesenchymal Stromal Cells* ; Necrosis ; Phenotype* ; Phosphorylation ; Prostaglandin-Endoperoxide Synthases

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Stemness Signature of Equine Marrow-derived Mesenchymal Stem Cells.

Morteza ZAHEDI ; Abbas PARHAM ; Hesam DEHGHANI ; Hossein Kazemi MEHRJERDI

International Journal of Stem Cells.2017;10(1):93-102. doi:10.15283/ijsc16036

BACKGROUND: Application of competent cells such as mesenchymal stem cells (MSCs) for treatment of musculoskeletal disorders in equine athletes is increasingly needed. Moreover, similarities of horse and human in size, load and types of joint injuries, make horse as a good model for MSCs therapy studies. This study was designed to isolate and characterize stemness signature of equine bone marrow-derived mesenchymal stem cells (BM-MSCs). METHODS: BM of three mares was aspirated and the mononuclear cells (MNCs) were isolated using density gradient. The primary MNCs were cultured and analyzed after tree passages (P3) for growth characteristics, differentiation potentials, and the expression of genes including CD29, CD34, CD44, CD90, CD105, MHC-I, MHC-II and pluripotency related genes (Nanog, Oct-4, Sox-2, SSEA-1, -3, -4) using RT-PCR or immunocytochemistry techniques. RESULTS: The isolated cells in P3 were adherent and fibroblast-like in shape with doubling times of 78.15 h. Their clonogenic capacity was 8.67±4% and they were able to differentiate to osteogenic, adipogenic and chondrogenic lineages. Cells showed expression of CD29, CD44, CD90, MHC-I and Sox-2 while no expression for CD34, MHC-II, CD105, and pluripotency stemness markers was detected. CONCLUSIONS: In conclusion, data showed that isolated cells have the basic and minimal criteria for MSCs, however, expressing only one pluripotency gene (sox-2).
Antigens, CD15 ; Athletes ; Bone Marrow ; Horses ; Humans ; Immunohistochemistry ; Joints ; Mesenchymal Stromal Cells* ; Trees

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Effects of Canine and Murine Mesenchymal Stromal Cell Transplantation on Peripheral Nerve Regeneration.

Diego Noe Rodriguez SANCHEZ ; Matheus BERTANHA ; Thiago Dias FERNANDES ; Luiz Antônio DE LIMA RESENDE ; Elenice DEFFUNE ; Rogério Martins AMORIM

International Journal of Stem Cells.2017;10(1):83-92. doi:10.15283/ijsc16037

BACKGROUND AND OBJECTIVES: Maintaining a permissive microenvironment is essential for adequate nerve regeneration. Cell-based therapy has the potential based cell replacement and promotion of axonal growth. The adipose tissue derived mesenchymal stromal cells (Ad-MSC) attract interest because neuroregenerative and anti-inflammatory properties. The aim of this study was to evaluate the effects of canine and murine Ad-MSC transplantation on the sciatic nerve regeneration. METHODS: Forty Wistar rats were divided randomly into: control group - CG (n=8); denervated group - DG (n=8); decellularized vein group - VG (n=8); decellularized vein+canine MSC–cMSC (n=8); descellularized vein+murine MSC–mMSC (n=8). After 10-mm nerve gap, the tubulation technique was performed with decellularized vein filled with 10⁶ MSC labeled with quantum dots (Qtracker 665®). The sciatic nerve functional index (SFI) and electroneuromyography (ENMG) measurements were carried and morphometric and immunohistochemistry analysis of the tissue. RESULTS: The SFI values were higher in the cMSC and mMSC groups at day 27 (p<0.020) and day 35 (p<0.011). The ENMG analysis also revealed better results in the mMSC group. Density, number, and total area of the fibers were increased in the mMSC and cMSC groups. Brain-derived neurotrophic factor BDNF and S-100 protein positive immunoreactivity showed a higher expression for both in the nerve of the mMSC and cMSC groups. The MSC labeled with quantum dots were detected at day 35, indicating neuronal survival long after the nerve damage. CONCLUSIONS: Murine and canine Ad-MSC associated with decellularized vein scaffold had positive effects on sciatic nerve regeneration in rats.
Adipose Tissue ; Animals ; Axons ; Brain-Derived Neurotrophic Factor ; Immunohistochemistry ; Mesenchymal Stromal Cells* ; Nerve Regeneration ; Neurons ; Peripheral Nerves* ; Quantum Dots ; Rats ; Rats, Wistar ; Regeneration* ; Regenerative Medicine ; S100 Proteins ; Sciatic Nerve ; Veins

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Osteogenic Differentiation of Bone Marrow Stem Cell in Poly(Lactic-co-Glycolic Acid) Scaffold Loaded Various Ratio of Hydroxyapatite.

Hyeongseok KIM ; Hye Min KIM ; Ji Eun JANG ; Cho Min KIM ; Eun Young KIM ; Dongwon LEE ; Gilson KHANG

International Journal of Stem Cells.2013;6(1):67-74.

BACKGROUND AND OBJECTIVES: Hydroxyapatite has biocompatibility and bioactivity and similar to bone of in human body. The purpose of this study is to evaluate osteogenic differentiation of bone marrow stem cell (BMSC) in PLGA Scaffold added various ratio of hydroxyapatite (HAp). METHODS AND RESULTS: PLGA and PLGA/HAp scaffold were prepared using solvent casting/salt-leaching method. BMSC was seeded on the PLGA and PLGA/HAp scaffold and the samples were cultured in 37degrees C incubator with 5% CO2 for 28 days. Alkaline phosphatase (ALP) was carried out to evaluate alkaline phosphatase activity at 1, 3, 7, 10 and 14 days. Alizarin Red S stating was performed to identify calcium in scaffold at 1, 7, 14, 21 and 28 days. Compressive strength was measured to evaluate mechanical property of scaffold. To confirm cell viability, MTT was carried out at 1, 3, 7, 14 and 28 days. RT-PCR was performed to verify specific marker expression of osteoblast and stem cell at 7, 14, 21 and 28 days. CONCLUSIONS: Osteogenic differentiation of BMSC was confirmed through ALP, RT-PCR, and alizarin red S staining in this study. These results suggest that HAp helps osteogenic differentiation of BMSC.
Alkaline Phosphatase ; Anthraquinones ; Bone Marrow ; Calcium ; Cell Survival ; Compressive Strength ; Durapatite ; Human Body ; Incubators ; Lactic Acid ; Osteoblasts ; Polyglycolic Acid ; Seeds ; Stem Cells

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Histological Study on Effect of Mesenchymal Stem Cell Therapy on Experimental Renal Injury Induced by Ischemia/Reperfusion in Male Albino Rat.

Eman Mostafa SADEK ; Noha Mohamed AFIFI ; Lamiaa Ibrahim ABD ELFATTAH ; Manal Ali ABD EL MOHSEN

International Journal of Stem Cells.2013;6(1):55-66.

BACKGROUND AND OBJECTIVES: Acute kidney injury (AKI) represents a major clinical problem with high mortality and limited treatment protocols. This study was planned to evaluate the therapeutic effectiveness of bone marrow-derived mesenchymal stem cells (BM-MSCs) in a rat model of ischemia/reperfusion (I/R) AKI. METHODS AND RESULTS: This study was carried out on thirty adult male albino rats. Animals were divided equally into three groups. Group I (control sham-operated group) (n=10), were subdivided equally into two subgroups; Ia and Ib. The experimental group (n=20) were all subjected to I/R injury by clamping both renal pedicles for 40 minutes. Half of the I/R animals did not receive MSC therapy (group II) [non-MSC treated group]. The other half of the I/R animals received single intravenous injection of PKH26 labelled BM-MSCs immediately after removal of the clamps and visual confirmation of reflow (group III) [MSC treated group]. Animals were sacrificed 24 hrs (subgroups IIa & IIIa) and 72 hrs (subgroups IIb & IIIb) after intervention. Serological measurements included serum urea and creatinine. Kidney specimens were processed for H&E, PAS and PCNA. Mean % of renal corpuscles with affected glomeruli, mean % of affected tubules, mean area % of PAS-positive reaction and mean area % of PCNA immunoreactivity were measured by histomorphometric studies and statistically compared. MSCs-treated group exhibited protection against renal injury serologically and histologically. CONCLUSIONS: Results of the present study suggest a potential reno-protective capacity of MSCs which could be of considerable therapeutic promise for cell-based management of clinical AKI.
Acute Kidney Injury ; Adult ; Animals ; Clinical Protocols ; Constriction ; Creatinine ; Humans ; Injections, Intravenous ; Kidney ; Male ; Mesenchymal Stromal Cells ; Organic Chemicals ; Proliferating Cell Nuclear Antigen ; Rats ; Urea

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New Approach of Bone Marrow-Derived Mesenchymal Stem Cells and Human Amniotic Epithelial Cells Applications in Accelerating Wound Healing of Irradiated Albino Rats.

Samah S MEHANNI ; Noha F IBRAHIM ; Alyaa R HASSAN ; Laila A RASHED

International Journal of Stem Cells.2013;6(1):45-54.

BACKGROUND AND OBJECTIVES: Irradiated wound healing is a highly complex and dynamic process. The latest technology making a huge difference in this process is stem cell therapy. The goal of this study was to evaluate the use of bone marrow-derived mesenchymal stem cells (BM-MSCs) or human amniotic epithelial cells (HAECs) in the healing of irradiated wounds. METHODS AND RESULTS: Forty five male albino rats were subjected to whole body 6 gray gamma radiations. One day post irradiation, full-thickness incisional wound was created in the tibial skin. The rats were randomly equally divided into three groups. The incisions of the first group (gp I) were injected intra-dermally with saline before stitching and those of both the second (gp II) and the third groups (gp III) were intradermally injected with BM-MSCs and HAECs before stitching respectively. Animals were sacrificed after the third, seventh and fourteenth days postoperative. The healing process was assessed histopathologically. CXCL-5, SDF-1 and Transforming growth factor-beta 1 (TGF-beta1) expression were also detected in biopsies from all wounds. Expression of TGF-beta1 in gp I was more than the other groups leading to severe inflammation, deficient healed dermis and delayed reepithelialization. SDF-1 expression was high in gp II while CXCL-5 expression was high in gp III causing accelerated wound healing. BM-MSCs showed a great effect on the quality of the dermis, while superiority of the epithelium and its appendages were achieved in HAECs group. CONCLUSIONS: Using BM-MSCs and HAECs could be used safely in case of irradiated wounds.
Animals ; Biopsy ; Dermis ; Epithelial Cells ; Epithelium ; Gamma Rays ; Humans ; Inflammation ; Male ; Mesenchymal Stromal Cells ; Rats ; Skin ; Stem Cells ; Transforming Growth Factor beta1 ; Wound Healing

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