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Clinical Research Progress of Traditional Chinese Medicine on Treatment of Diabetic Nephropathy

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):95-97. doi:10.3969/j.issn.1005-9903.2009.07.036

Clinical research progress of Traditional Chinese Medicine(TCM) on treatment of diabetic nephropathy in recent ten years was explained from aspects of pathogen and pathogenesis, patterns classification of TCM, Chinese herbal Compound, single drug and the extractive. And the result showed a good therapeutic effect on early stage of TCM treatment for diabetes mellitus which was worthy to be popularized.

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The Protective Effect of Rhodobryum giganteum (Schwaegr.) Par. on Umbilical Vascular Endothelial Cells and the Influence on Secretion of NO and NOS

Ying CAI ; Qunli WEI ; Xiaohe LU

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):79-82. doi:10.3969/j.issn.1005-9903.2009.07.030

Objective: To study the effect and mechanism of Rhodobryum giganteum (Schwaegr.) Par. 's anti-atherosclerotic effect. Methods:Vascular endothelial ceils were cultivated and the H2O2 were used to induce the oxidative stress injury of human umbilical vascular endothelial cells (HUVEC). Water extract of Rhodobryum giganteum (Schwaegr.) Par. Was added to cultivated HUVEC, and the activity of the cells was carefully determined by OD value with MTF method. The Griess Reagent was used to detect the NO concentration of different groups. At the same time, the NO fluorescence analysis probe, DAF-FM DA. (3-amino, 4-aminomethyl-2', 7'-difluorescein, diacetate), was used to determine the activity of NO synthase. Results:The most suitable stimulating concentration of H2O2 on HUVEC is 12.5 mmol· L-1. Water extract of Rhodobryum giganteum (Schwaegr.) Par. Was co-cultured with HUVEC damaged byH2O2 .The OD values indicate that 3.33,2.50 mg· mL-1 water extract groups increase activity of the cells significantly compared with the model group (P <0.05) ,and 3.33 mg·mL-1 is much better than 2.50 mg·mL-1 dose group. To determine the content of NO and NOS, the 2.50 mg·mL-1 dose has significant effect (P < 0.05). Conclusions: The H2O2 concentration of 12.5 mmol· L-1 could be used to establish the injured model of endothelial cell successfully. 2.50 and 3.33 mg·mL-1 water extract of Rhodobryum giganteum (Schwaegr.) Par. Have protective effect on ECV304 injured by H2O2. 2.50 mg· mL-1 water extract of Rhodobryurn giganteum (Schw aegr.) Par could increase the activity of NOS and promote the synthesis and secretion of NO.

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The Pathological Study on Effective Compounds of TongMai in Treating MsPGN in Rats

Mingping LIU ; Zhaosheng HUANG ; Nizhi YANG ; Pingqing WEI ; Qingguang WU

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):54-57. doi:10.3969/j.issn.1005-9903.2009.07.021

Objective: To observe the therapeutic effects of effective compounds which were extracted from TongMai. Methods: SD rats experimential model with MsPGN were induced by subcutaneously injection rabbit anti-rat thymocyte serum(ATS) and forced swim. The rats were respectively ig with different dose(1.2,0.6,0.3 g'kg-1) of effective compound of TongMai for 4 weeks. Then the proliferative degree of mesangium was examined by HE stain, PAS stain and immunfluorescent respectively. Mesagium area proportion in glomerular capillaries area was calculated using image analyzing system. The expression of PCNA in the glomeruli was examined by immunohis to chemistry. Results: The effective compounds of TongMai can control the hyperplasia of the glomemlar mesangial cells (GMG)and the mainifold of the mesangial matrix, and prevent the deposit of IgG in mesangium. The proportions in glomerular capillaries area in model control group were significantly higher than that in the treatment groups; The express of proliferative cell nuclear antigen (PCNA) were ascended either. Conclusion: The effective compound of TongMai can improve the damage s of pathology in glomerular.

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Studies on Qualitative and Quantitative Methods of Shenbai Concentrated Pills

Xiaowei DU ; Wenwei ZHANG ; Dan YU ; Hongxin CAO ; Yan LIU ; Peican WU

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):19-22. doi:10.3969/j.issn.1005-9903.2009.07.008

Objective:To study the qualitative and quantitative methods of Shenbai concentrated pills. Methods:Danshen root and lightyellow sophora root in Shenbai concentrated pills were identified with the method of TLC. The contents of astragaloside Ⅳ and paeoniflorin were determined with the method of HPLC. Results: For TLC, the spots of the two herbal drugs were well separated and without interference. Quantitative analysis of HPLC showed that the average recovery of astragaloside 1V was 100.82% (RSD1.73%) with the RSD values of precision, repeatability and stability tests were 2.79%, 1.48% and 1.44%, respectively; and the average recovery of paeoniflorin was 99.83% (RSD2.34%) with the RSD values of precision, repeataibility and stability tests were 2.25%, 2.16% and 1.60%, respectively. Conclusions: The methods set up by this study are accurate and easy to perform with the merits of good resolution, specificity and reproducibility. It could effectively control the quality of Shenbai concentrated pills.

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Studies on Qualitative and Quantitative Method of Gubaoxinshen Tablets

Yan ZHANG ; Xiangqun JIN ; Guangshu WANG

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):14-16. doi:10.3969/j.issn.1005-9903.2009.07.006

Objective:To establish the qualitative and quantitative methods for Gubaoxinshen Tablets. Method: Soy bean, and epimedium herb were identified by TLC, and the content of genistein and icarrin were determined by HPLC. Result: The developed TLC sports were was fairly clear, the HPLC method showed good repeatability. The average recovery of genistein was 98.9% with RSD 0.9%, and the average recovery of icarrin the was 98.0% with RSD 1.0%. Conclusion: The method is simple, accurate and can becauseofon control quality of Gubaoxinshen Tablets.

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Analytical methods for quality control of Fructus Forsythiae and its Preparations

Yan XIONG ; Jingjing ZHU ; Zhiming WANG ; Limei LIN ; Bohou XIA

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):97-99. doi:10.3969/j.issn.1005-9903.2009.07.037

This paper retrospectively analyzed the quality control methods of Fructus Forsythiae, summarized the corresponding achievements and problems on its quality control. It can provide some available envidences for the quality control of Fructus Forsythiae and its preparations.

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Anti-tumor Effect of Phellinus Linteus and Coriolus Versicolor Capsule on S180 sarcoma and H22 hepatoma in mice

Kunxing LI ; Xueping ZHU ; Hailin ZHANG ; Ke NIE ; Wei GUO

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):83-86. doi:10.3969/j.issn.1005-9903.2009.07.031

Objective: To observe the anti-tumor effect of Phellinus Linteus and Coriolus Versicolor Capsules (PLCVC) in S180 sarcoma and H22 hepatoma animal models in mice. Methods: The sarcoma S1180 and hepatoma H22 models were established in mice. After 12 days of treatment, the animals were killed, and the subcutaneous sarcoma were separated and weighted. The levels of vascular endothelial growth factor(VEGF), CD4 and CD8 of S180 tumor tissue were investigated by immunohistochemical method. KM mice were intraperitoneal injected with H22 hepatoma cells, and treated with different experimental drugs. The survival time was observed and recorded, and life-prolongation rate was calculated. Result: PLCVC could inhibit the growth of S180 and H22 tumor, and inhibit the expression of VEGF, improve the expression of CD4 and CD8. The survival time of the mice treated by PLCVC were significantly longer than the untreated group. Conclusion: PLCVC can inhibit the growth of tumour, the mechanism is partially related to inhibiting angiogenesis and improving the immunological function.

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Protective Effect of Astragalus Membranaceus on Ischemic Acute Renal Failure

Yuxin BAI ; Liang FU ; Jin GAO

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):74-76. doi:10.3969/j.issn.1005-9903.2009.07.028

Objective: To investigate the effect of astragalus on the serum levels of hepatocyte growth factor (HGF), renal function and the damage of renal morphology in ischemic acute renal failure (IARF). To study the protective mechanism of astragalus in IARF. Methods: The rats were pretreated with astragalus or vehicle for 3 days. IARF was induced by left renal artery clipping for 60 min, after the right kidney was removed. Biochemical parameters for serum HGF,blood urea nitrogen(BUN),creatinine(Cr) were measured and histological analyses of the kidneys were performed by tubular grading. Results: There have no significant difference between the serum HGF levels of I/R controls and the levels observed in sham-operated group (P > 0.05). Serum HGF levels in astragalus treated group was significantly increased than those of I/R control group(P < 0.01) .After 4 h of I/R, serum levels of BUN and Cr were increased in I/ R control rats compare with Sham-operated rats(P < 0.01). Astragalus treatment reduced the levels elevation(P < 0.05). Simultaneously,. I/R-induced severe damage in renal morphology were significantly ameliorated by astragalus treatment(P < 0.01). Conclusions: Astragalus pretreatment can alleviate the damage of renal function in IARF. It is may be one of the mechanisms of astragalus for protecting renal tissue in IARF that astragalus can induce the expression of HGF.

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Influence of Xiaokekang on Renal Function in Diabetic Nephropathy Rats

Dexian JIA ; Xiaomei JI ; Qian WANG ; Yu HAO ; Qiuxia ZHANG ; Xinlou CHAI ; Yingshu LIU ; Pengfei TIAN ; Weilian SU ; Liwei REN

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):57-59. doi:10.3969/j.issn.1005-9903.2009.07.022

Objective:To observe the changes of glyeemia, urine protein, serum creatinine, blood urea nitrogen and glycosylated hemoglobin in diabetic nephropathy rats and treating effects of Xiaokekang. Methods: Streptozotocin (STZ) was abdominally administered and was feeded by high lipid diet to establish diabetic nephropathy model in rats. Animals were divided into three groups: model group, Xiaokekang treatment group and normal group. Changes in glycemia, serum lipids, serum creatinine, blood urea nitrogen were measured at the 8th, 12th and 16th week after STZ injection. Glycosylated hemoglobin was measured at the 16th weeks after STZ injection. Results: Glycemia, serum lipids, serum creatinine were higher at the 8th, 12th and 16th week compared with normal group. Serum creatinine was higher at the 12th and 16th week, and glycosylated hemoglobin was also highter than that of the normal group. Xiaokekang reduced these changes. Conclusion: Xiaokekang can reduce glycemia, serum lipids and protect renal function.

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Study on Blood-stasis Syndrome Induced by Cold: Target on the Tongue

Zhenhua CAO ; Junxiang YIN ; Jinzhou TIAN ; Jing SHI ; Wenjie WANG ; Lejun LI ; Pengwen WANG ; Ying REN ; Zhihui ZHU ; Haiyun SUN

Chinese Journal of Experimental Traditional Medical Formulae.2009;(7):50-53. doi:10.3969/j.issn.1005-9903.2009.07.020

Objectives:To observe the changes of the tongues in rats with blood-stasis syndrome induced mainly by cold. To explore the evidence that tongues were the target of blood stasis syndrome and the target of medication. Methods: Rats with blood-stasis syndrome induced by cold (BSC group) were dipped into ice water (0 ℃) for 5 minutes every day and lasted 20d individually. Different drugs were given orally after the model establishment. And then we took photos of tongues of all rats by digital camera, analyzed the gray scale value of all rats' tongues using image analysis software, and observed the capillaries in tongues by electron microscope. Results: The tongues of rats which had been frozen in ice water for 20 d (once a day) were dark purple, the same as that of models induced by chemical materials .While as for the normal rats, the tongues were lustrous and ruddy. The changes of color of the tongues persisted about 1 week and were great obvious at the 3rd day after the model establishment among different time-points. After given different drugs, the degree of dark purple tongue degraded, showed significant difference (P < 0.01) from that of model rats. The capillary stegnosis and nucleus turgescence of vascular endothelial cell were observed in tongues in BSC group by using electron microscope. After medication, the above changes recovered. Conclusion: The degree of dark purple tongue indicates the degree of diseases with blood-stasis syndrome. The tongue probably is a target of drug treatment.

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