Chinese Journal of Applied Physiology.2002;18(1):13-100.
Chinese Journal of Applied Physiology
1985 to Present ISSN: 1000-6834
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Chinese Journal of Applied Physiology.2002;18(1):9-13.
AIMTo investigate the roles of acetylcholine (ACh) receptors in the rapid effects of corticosterone (CORT) on the presympathetic neurons in the rostral ventrolateral medulla (RVLM) of rats, and study the non-genomic mechanism of glucocorticoid (GC) in the integration of sympathetic cardiovascular activity.
METHODSThe effects of microelectrophoresis of CORT on the discharge of the presympathetic neurons in the RVLM were observed by extracellular recording in urethane-anaesthetized rats. The responses of atropine (a blocker for M type of ACh receptor, ATR), d-tubocurarine (a blocker for N1 type of ACh receptor, d-TC) and hexamethonium (a blocker for N2 type of ACh receptor, C6) to the effects of CORT on the presympathetic neurons were investigated respectively.
RESULTSTotally 33 presympathetic neurons in the RVLM were recorded. Among them the firing rate of 25 (76%) presympathetic neurons was increased by microelectrophoresis of CORT. The effects of CORT were also positively correlated with the currents. In the other 8 presympathetic neurons, had was shown no effect after microelectrophoresis of CORT. In 10 presympathetic neurons, which discharge was increased by CORT, microelectrophoresis of ATR decreased the firing rate of these presympathetic neurons (P < 0.05), and did not fully block the excitatory effect induced by CORT. In both 7 and 6 presympathetic neurons, application of d-TC and C6 had no effect on these neurons respectively, and did not fully block the excitatory effect induced by CORT.
CONCLUSIONCORT had rapid excitatory effects on the presympathetic neurons in the RVLM, which effect might be independent on ACh receptors.
Animals ; Cholinergic Antagonists ; pharmacology ; Corticosterone ; pharmacology ; Electrophoresis, Microchip ; Male ; Medulla Oblongata ; drug effects ; physiology ; Neuromuscular Nondepolarizing Agents ; pharmacology ; Neurons ; drug effects ; physiology ; Nicotinic Antagonists ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors, Cholinergic ; physiology
Chinese Journal of Applied Physiology.2002;18(1):5-8.
AIMTo study the effects of nitric oxide on calcium-activated potassium channels (KCa) in hypothalamus neurons.
METHODSThe data was recorded by adopting cell-attached or inside-out configuration of patch-clamp technique.
RESULTSNitric oxide was found to increase the open probability of the channels directly or through increasing cGMP, which resulted from both the prolongation of open period and increase of open frequency.
CONCLUSIONNitric oxide could regulate calcium-activated potassium channels in hypothalamic neurons through alternative pathways.
Animals ; Calcium ; metabolism ; Hypothalamus ; cytology ; Neurons ; metabolism ; Nitric Oxide ; metabolism ; Patch-Clamp Techniques ; Potassium Channels, Calcium-Activated ; metabolism ; Rats ; Rats, Sprague-Dawley
The effect of nitric oxide on electroacupuncture analgesia in central nervous system of rats.
Chinese Journal of Applied Physiology.2002;18(1):4-46.
Protein synthesis and characterization in isolated mitochondria from rat brain.
Chinese Journal of Applied Physiology.2002;18(1):1-4.
AIMTo establish a system of mitochondrial translation in vitro of rat brain and identify it's production of protein by molecular weight.
METHODSMitochondria isolated from hemisphere of rat brain by differential centrifugations. The optimization of mitochondrial translation in vitro by 3H-Lencine incorporation was explored. 35S-methionine labeled products of mitochondrial protein synthesis were identified by SDS-PAGE and fluorography.
RESULTSIsolated mitochondria had a highly activity oxidative phosphorylation and respiratory control ratio (RCR) was between 3.5 and 5.5. The activity of 3H-Lencine incorporation in isolated mitochondria in vitro increased with time of incubation in 60 min and maintained a steady level. The maximal activity of 3H-Lencine incorporation per milligram mitochondria protein occured at 1 mg mitochondria/ml of incubation mix.The major auto radiographic bands could be observed at 86, 68, 56, 43, 33, 29, 25 and 18(kD) molecular weight separated on SDS-PAGE.
CONCLUSIONThe translation system of rat brain mitochondria in vitro is faithful and high activity, can be used to study mtDNA expression and regulation in mammalian brain at the level of translation.
Animals ; Brain ; metabolism ; Electrophoresis, Polyacrylamide Gel ; Male ; Mitochondria ; metabolism ; Molecular Weight ; Protein Biosynthesis ; Rats ; Rats, Wistar
Chinese Journal of Applied Physiology.2005;21(4):377-381.
AIMTo investigate the dynamic changes and functions of urotensin II (U lI) receptor (UT) in pulmonary arteries of rats chronically exposed to hypoxia-hypercapnia.
METHODSIn rats with hypoxia-hypercapnia at 1, 2 and 4 weeks U II receptor binding of pulmonary arteries sarcolemma was determined by radioligand assay. U II mRNA and UTmRNA in various grades of pulmonary arterioles were measured by in situ hybridization.
RESULTS(1) Mean pulmonary pressure (mPAP) and weight ratio of right ventricle to left ventricle and septum (RV/LV + S) of 1-week group were higher than those of normal control (NC) group by 26.2% and 21.6% (P < 0.01), respectively, and 2-week group higher than 1-week group by 22.5% and 14.1% (respectively, P < 0.01). However, no significant changes were found between 4-week and 2-week group. (2) U Il receptor (Bmax) of 1-week group was higher than NC group by 38.8%, 2-week group higher than 1-week group by 23.2%, and 4-week group increased 7.3% compared with 2-week group (respectively, P < 0.01). The UT changes were time-dependent, while the affinity to U II (Kd) was no different among each group. (3) UII mRNA in each grade of pulmonary arterioles of 2-week group and 4-week group were higher than NC group (respectively, P < 0.01), and those of 2-week group were higher than 1-week group by 5.9% (P > 0.05), 16.4% and 9.1% (respectively, P < 0.01), while no differences existed between 2-week group and 4-week group. (4) UT mRNA in each grade of pulmonary arterioles of all hypoxia-hypercapnia groups was higher than NC group (respectively, P < 0.01), and those of two abaxial grade vessels in 1-week group were the highest. No differences existed between 2-week group and 4-week group. (5) The pulmonary vessels remodeling were time-dependently aggravated by hypoxia-hypercapnia.
CONCLUSIONThe dynamic changes of UT in pulmonary arterioles might have important contribution to the development of pulmonary hypertension and pulmonary arteriole remodeling induced by chronic hypoxia-hypercapnia in rats.
Animals ; Arterioles ; metabolism ; physiopathology ; Hypercapnia ; metabolism ; Hypertension, Pulmonary ; metabolism ; physiopathology ; Hypoxia ; metabolism ; Male ; Pulmonary Artery ; metabolism ; physiopathology ; Rats ; Rats, Sprague-Dawley ; Receptors, G-Protein-Coupled ; metabolism
Chinese Journal of Applied Physiology.2005;21(4):375-422.
Animals ; Calcium ; metabolism ; Cell Hypoxia ; Cells, Cultured ; Hippocampus ; cytology ; Neurons ; drug effects ; metabolism ; Rats ; Rats, Wistar ; omega-Conotoxins ; pharmacology
Chinese Journal of Applied Physiology.2005;21(4):371-375.
AIMTo determine if the diagnostic ultrasound and self-made microbubbles could be used to increase gene transfection and expression in cardiac myocytes by means of the ultrasound-mediated microbubbles destruction.
METHODSThe perfluoropropane-exposed sonicated dextrose albumin(PESDA) microbubbles were made and mixed with indicated volume reporter gene encoding beta-galactosidase prior to gene transfection. Gene transfection into the cultured cardiac myocytes was performed by exposure to the various intense diagnostic ultrasound (1.3 MHz) in the presence of the gene-attached microbubbles. The calcium phosphate precipitation gene transfection was carried out alone or in combination with ultrasound-mediated destruction microbubbles. The cells were harvested 48 h after transfection and beta-galactosidase expression was detected by in situ staining and quantitive assay.
RESULTSCardiac myocytes exposed to ultrasound with PESDA induced significantly increase in gene expression (60-fold compared with naked plasmids transfection, P < 0.01). Moreover, it was found that the reporter gene expression not only related with ultrasound intension but also with the microbubbles concentration. In combination with calcium phosphate precipitation gene transfection, ultrasound-mediated destruction microbubbles resulted in more intense gene expression even 6 hours after calcium phosphate precipitation gene transfection.
CONCLUSIONThe ultrasonic destruction of gene-loaded microbubble is a highly effective gene transfer method, and it not only acts on the gene entry into cells, but also on the intracellular exogenous DNA expression.
Animals ; Gene Expression ; Genes, Reporter ; Myocytes, Cardiac ; cytology ; Plasmids ; Rats ; Rats, Wistar ; Transfection ; methods ; Ultrasonics
Chinese Journal of Applied Physiology.2005;21(4):367-370.
AIMTo detect effect of the different frequency of chronic electrical stimulation (CES) on myofibrillar isoform, myosin heavy chain (MHC) and metabolic enzyme activities.
METHODSThe histochemical method and SDS-polyacrylamide gel electrophoresis were respectively employed.
RESULTS(1)There were a significant increase in I myo-fibrillar isoform and I MHC isoform and decrease in II B myofibrillar isoform and II B MHC isoforms in the chronic low frequency electrical stimulation (CLFES) 10 Hz and 20 Hz groups, but opposite results were found in the chronic high frequency electrical stimulation (CHFES) 50 Hz and 100 Hz groups. (2) There were a significant increase in the aerobic-oxidative enzyme activities and capacity, and a concomitant significant drop in glycolysis enzyme activities in CLFES groups, but opposite results were found in CHFES 50 Hz and 100 Hz groups.
CONCLUSIONIt was suggested that there was a significant dependent relation between chronic electrical stimulation frequency and myofibrilla isoforms, myosin heavy chain (MHC) and metabolic enzyme activities.
Adaptation, Physiological ; Animals ; Diaphragm ; enzymology ; metabolism ; physiology ; Electric Stimulation ; Muscle Contraction ; Myosin Heavy Chains ; metabolism ; Nonmuscle Myosin Type IIB ; metabolism ; Protein Isoforms ; Rabbits
Effects of leptin on RNA content of LHA, VMH, PVN and the body fat.
Chinese Journal of Applied Physiology.2005;21(4):365-456.
Adipose Tissue ; Animal Feed ; Animals ; Hypothalamus ; drug effects ; physiology ; Leptin ; pharmacology ; Male ; Mice ; Mice, Inbred Strains ; RNA ; analysis
Country
China
Publisher
中国生理学会
Editor-in-chief
tjzgyish@163.com
Abbreviation
Chinese Journal of Applied Physiology
Vernacular Journal Title
中国应用生理学杂志
ISSN
1000-6834
EISSN
Year Approved
2013
Current Indexing Status
Suspended(2024)
Start Year
1985
Description
1986:应用生理学杂志; 1987-:中国应用生理学杂志
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