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The variance of PKC in cardiac cells of SMDS victims

Hongquan XU ; Hualan JING ; Yijun ZHANG ; Jiquan YAN ; Yongxi WEN ; Hong LIN ; Fuxue JIANG

Chinese Journal of Forensic Medicine.2009;24(6):396-397,后插1.

Objective This article aims to explore the relationship between PKC and SMDS(sudden manhood death syndrome,SMDS).Methods By way of immunohistochemical staining of left,right ventricular tousles,sino atrial node and atrioventricular node from 30 cases of SMDS group and 20 cases of control group,we observed and measured the expression and the intensity variance of PKC in myocardial ceHs with graphical analysis software.Results The average gray value and total area of PKC in myocardial cells of cases in the SMS group are obviously higher than that of cases in control group(P<0.05).And in the SMDS group,the average gray value of PKC in sino atrial node is higher than that in atrioventricular node.Conclusion As one way of humoral regulation,PKC is involved in the process of protecting the heart from impairment in SMDS.and it can be considerated as a reference index to identify the SMDS.

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DISCUSSION ON PRIMARY SUBARACHNOID HEMORRHAGE:REPORT OFSEVEN AUTOPSY CASES

Yongchang MA

Chinese Journal of Forensic Medicine.1986;0(02):-.

Prim ary subarachnoid hemorrhage(SAH)is not uncommon in forensic autopsy.This paper reported 7 cases of primary SAH.Disputes about the cause of death arose because death occured after minor head injuries.Through thorough histopathological examination,causesof primary SAH in these 7 ca- ses were determined:arteriovenous malformation of the brain in 5 cases; aneurysm in 1 case;giant cell arteritis in 1 case.Causes of SAH were undeter- mined macroscopically in all these cases.The misdiagnosis of traumatic SAH were made in 4 reappraisal cases.Facts indicated the importance of thorough histopathological examination tor determining real causes of SAH. The literature was reviewed and the methods of histopathological exa- mination,as well as the attention it must paid were discussed.

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Gc SUBTYPING IN THE HAN POPULATION IN CHENGDU AND PHENOTYPING OF Gc IN HUMAN BLOODSTAINS

Qu ZHAO ; Meiyun WU

Chinese Journal of Forensic Medicine.1986;0(02):-.

0.05).The exclusion probability of Gc is 34.8% and discrimination probability of Gc is 79.44%.There are not any significant difference of the distribution of Gc subtypes between the Hun population in Chengdu and those in Hong Kong and Japanese.The difference of the distribution of Gc subtypes between the Han population in Chengdu and those in Malaysia,Indonesia,India as well as the American caucasians,Belgians,Icelander and West German are sig- nificant. The phenotyping of Gc in 11 bloodstain samples kept in room temperature for twenty weeks were carried out successfully also using PAGIF followed by immunofixation method.

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ISOLATION AND PURIFICATION DF Gc PROTEIN FROM HUMAN PLASMA

Yiping HOU ; Meiyun WU

Chinese Journal of Forensic Medicine.1986;0(02):-.

Group-specific component(Gc), one of the polymorphic proteins of human plasma,has been isolated and purified from human plasma of Gc 2-2 phenotype by a procedure including DEAE-Sephadex-A50,Sephadex G100 and DEAE-Sephadex-A50 chromatography. The purified Gc identified by PAGE,SDS-PAGE and immunoelectrophoresis was homogeneous and reacted specifically with the commercial anti-Gc serum (DAKO) kit. The molecular weight of the purified Gc was about 58 kd.

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AN EXPERIMENTAL STUDY OF TIMING OF WOUND

Qiunian SHI ; Wei GONG

Chinese Journal of Forensic Medicine.1986;0(02):-.

Experimental incised wounds 8 mm in diameter were made on the right side of the back of 48 rats under anesthesia using aseptic technique. All animals were randomly divided into 12 groups according to the time of wound biopsies which were taken immediately after skin cutting, lh,12h,24h,2,3,4,5,6,7,8,9 and 10 days after wounding.One rat of each group was given colchicine before animal sacrifice in order to observe cell mitosis. Skin specimens were fixed in 10% formalin,embeded in paraffin and sectioned for HE stain,Gorden and Sweet stain for reticular fiber,Weigert stain for elastic fiber,PTAH stain for fibrin and Van Gieson stain.Macroscopically, wounds were getting smaller daily,and reduced in size markedly on the 6～7th day. The histologic changes were as follows:Acute inflammation on the wall of wounds occurred 1h after skin cutting;profuse fibrin exudation at the top of the base and on the wall of wounds at 24h;new capillary formation and regeneration of the epidermis on the 2nd day;granulation tissue formation and collagen fiber deposition on the 3rd day;small artery formation in the granulation tissue on the 4～5th day;granulation tissue maturation and the regenerating tissue covering the whole wound area on thd 6～7th day;the scar formation and contraction on the 8～9th day;further sear contration on the 10th day.The epidermis regenerated from the proliferation of the epidermis at the edge of the wound with the speed of 1.23mm/day. The regeneration of the epithelium proceeded under the necrotic tissue on both the wall and at the base of wounds. The mechanism of the wound contraction was discussed.

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ISOLATION AND ANALYSIS OF SINGLE HAIR KERATIN

Xuemin HE

Chinese Journal of Forensic Medicine.1986;0(02):-.

The protein components from single hair(1～5 cm in length)have been analysedby using mini SDS-gradient polyacrylamide gel electrophoresis(5.0～17.0%).Thekeratins from hairs of human,pan,comopithecus,hylobates,oryctolagus and caviawere compared.Keratin in human hair differed from the animal hairs in the lowmolecular weight components.The low-sulphur protein and high-sulphur proteinfrom hairs were determined by means of N-(3-pyrene)maleimide labelled hair proteins.The molecular weight of the former was within the range of 45,000～52,000 daltonwhile that of the latter within the range of

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EXAMINATING THE SPERM ANTIGEN IN SEMINAL FLUID AND SEMINAL STAIN USING ELISA METHOD

Yaqing DUAN ; Jinghua XU ; Xiaofeng WANG ; Guanshi ZHANG ; Yuxin LI

Chinese Journal of Forensic Medicine.1988;0(04):-.

The sperm antigens were examined using the method of ELISA and specific monoclonal antiboby against human sperm.The results of ELISA method of 10 fresh seminal fluid samples and 15 seminal stains showed 100% positive rate. Even diluted the fresh seminal fluid to 10~6 times of seminal stains extract to 51?0~5 times,both were positive also.However,it was negtive for testing other specimens such as saliva stains,urine stains,colostrum stains,sweat stains, vaginal secretion stains and human seminal stains after vesoligation. The result certificate that using specific antibody to the sperm,the ELISA method is high sensitive and specific for the sperm antigen assay.

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DETERMINATION OF CARBOXYHEMOGLOBIN IN BLOOD BY TWO-WAVELENGTH SECOND-DERIVATIVE SPECTROPHOTOMETRY

Xixuan ZHANG ; Chuichang FAN ; Jian YU

Chinese Journal of Forensic Medicine.1988;0(04):-.

A new method of determination of COHb is reported in this paper by two- wavelength second-derivative spectrophotometry.Blood was diluted with a solution of THAM containing Na_2S_2O_4.The second-derivative spectrum was measured in the region of 500-600 nm and the derivative absorbance differerce ?Ax was deter mined at the wavelengths of 564 nm and 576 nm.Then the solution was satura- ted with CO gas.The second-derivative spectrum of COHb was measured in the same region.The derivative absorbance difference ?A_(100) was determined at the same wavelengths.The COHb in blood was calculated by the ratio of ?Ax to ?A_(100).The results of determination of COHb in standard blood samples were in good agreement with the theoretical values.

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AN EXPERIMENTAL MODEL OF CEREBRAL CONCUSSION GUINEA, PIG AND THE MORPHOLOGICAL CHANGES

Fengsheng YAO

Chinese Journal of Forensic Medicine.1986;0(01):-.

An experimental model of cerebral concussion induced by blunt force stroke in 46 guinea pigs was established. 30 out of 46 animals were unconscious immediately after stroke followed by recovery Within few minutes without any seuqel in the posttraumatic period The animals were killed at 3,6,12,24,48 and 72 hours, 1,2,3,4 weeks after injury. Within 72 hours after injury, some of axons of both the afferent and the efferent nerve fibers of the corpus callosum and the brain stem were swelling like necklace or disrupted into segments macrophage infiltration, glosis, satellitosis, pyknosis of nuclioli of neuron, lysis of nissles bodies, vascular reaction as well as the retraction of the torn ends of axon as demonstrated by the silver stain were observed in animals killed 24 and 48 hours after injury. The reaction mentioned above became milder and milder 1～4 weeks after injury. Brain contusion were produced in other 16 experimental animals. In 7 animals of control group, no change was observed

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THE RELATIONSHIP OF FIBRONECTIN DETECTION AND TIMING OF EARLY RAT'S SKIN WOUNDS

Yaonan MO

Chinese Journal of Forensic Medicine.1986;0(02):-.

Immunohistochemical PAP method was used to detect the fibronectin (FN) in the rat's skin incised wounds. The results demonstrated that FN manifest itself in the wall of the wounds after 15 min injury, within 1 to 8 hrs after injury, FN accumulated numerously, forming a gradient deposition. In contrast, no FN was found in wounds formed 5 min after death. The research had eatablished a new method for differentiation timing of early wound, and for differentiation of antemortom from postmortem wounds

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