Objective To analyze and evaluate the 48 selected highly polymorphic SNP loci in Chinese Han population on genetic and forensic aspects.Methods Samples from 200 unrelated Han individuals in East China were collected.48 X-SNP polymorphic genetic markers were selected according to the information providedby NCBI and HapMap,and then were typed by SNPlex~(TM) System for establishing genetic data.Results The 48 X-SNP loci were highly polymorphic markers in East China Han population except rs6527549,and their polymorphism information contents(PIC)were all above 0.32,the discrimination power(DP)in females and males were above 0.56 and 0.40 respectively,the probability of exclusion(PE)in duos and trios were above 0.20 and 0.32 respectively.In addition,linkage disequilibriums were observed among some loci.Conclusion The 48 X-SNP loci union is suitable for developing high-throughout automated research and also useful for special parentage testing.