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Effect of disequilibrium of calcium homeostasis on apoptosis of MDCC MSB1 cells induced by lanthanum chloride

Lan ZHAO ; Wenjun LI ; Jintao WANG ; Shiwen XU

Chinese Journal of Veterinary Science.2009;29(5):643-648.

To investigate the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride(LaCl3 ), MDCC-MSB1 cells was conventionally cultured in RPMI1640, which was tured for 24 h, MTT was utilized to detect the cell multiplication inhibition ratio,DNA ladder and TUNEL to detect apoptosis,the Fura-2/AM as the probe to detect the [Ca2+]i within cells. The results indicated when LaCl3 concen and [Ca2+]i also increased,and presented a dose-effectiveness relationship. LaCl3 could restrain the proliferation of MDCC-MSB1 cells,suggesting their apoptosis induced by changing [Ca2+]i.

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Effects of dietary energy level on ovarian expression of mRNA s for luteinizing hormone receptor and follicle-stimulatiing hormone receptor in prepubertal gilts

Xu ZHOU ; Miaoying YU ; Liwen LIU ; Kangle YI ; Chunjin LI ; Lu CHEN ; Yanling SUN

Chinese Journal of Veterinary Science.2009;29(1):97-105.

To explore the effects of long-term treatment of different dietary energy levels on ovarian expression of mRNAs for LHR and FSHR,the present study was performed in nine growth-matched littermate crossbred (Land-race×Large White X Duroc) prepubertal gilts. At approximately 30 kg of body weight and 50 day of age,gilts were housed with individual feeding stalls and placed on a normal level of feeding for 90 days (dl-90) with free ac-cess to water and food throughout the whole research. From d91 ,littermates were divided and randomly assigned to one of the following three treatment lines for 3 weeks till d112:Group H,Group M, and Group L, fed the high energy level diet (n = 3, digestible energy 14.87 MJ/kg), moderate energy level diet (n = 3, digestible energy 12.39 MJ/ kg), and low energy level diet (n = 3, digestible energy 9.98 MJ/kg), respectively. When gilts were slaughtered on d112 after 3 weeks energy treatment, both ovaries of every gilts were collected,snap frozen in liquid nitrogen and re-tained at -80℃ for use to determine and analysis the relative amount of ovarian LHR and FSHR mRNAs using semi-quantitative RT-PCR. Results showed that the effect of dietary energy treatment was notable: the ovarian ex-pression of mRNAs for LHR and FSHR was significantly higher (P<0.05) in gilts treated with high energy diet compared to gilts treated with moderate and low energy diets, while gilts treated with low energy diet had a signifi-cantly lower (P<0.05) ovarian LHR and FSHR expression compared with gilts treated with moderate and high en-ergy diets. These results revealed that ovarian expression of I.HR and FSHR in prepubertal gilts increased as the lev-el of dietary energy intake elevated,i, e. , high energy diet can markedly enhance ovarian expression of mRNAs for LHR and FSHR,whereas energy deficit markedly suppress the expression.

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Expression patterns of neuroendocrine cells in the duck thymus

Jing FANG ; Hengmin CUI ; Min HE

Chinese Journal of Veterinary Science.2008;28(4):446-450.

The expression patterns of 5-hydroxytryptamine(5-HT),gastrin(Gas),β-endorphin(End),somatostatin(SS),vasoactive intestinal peptide(VIP),glucagon(Glu) and neuropeptide Y(NPY)-positive cells in the duck thymus were studied by the immunohistochemical method associated with image analysis.The result shows that these 7 kinds of neuroendocrine cells with strong intensity were differentially detected in the duck thymus.The End-positive cells were abundantly expressed in the thymic cortex,whereas the Gas,5-HT,SS,VIP,Glu and NPY ones were more frequent in the medullary and around the corticomedullary junction.Except Glu,the other 6 kinds of neuroendocrine substances with various intensity were expressed in the cells of thymic corpuscles.The observation demonstrated that the duck thymus is not only a primary lymphatic organ,but also has important neuroendocrine functions.The End-positive cells in the thymus may play crucial roles in the development of immature T-lymphocytes.The neuroendocrine cells with a predominant distribution in the medulla and around the corticomedullary junction might facilitate exerting their influence via an endocrine,autocrine or paracrine pathways on the function of mature T-cells.The possible roles of thymic corpuscles are also discussed.

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Changing trends in the development of the thymus in duckling

Jing FANG ; Hengmin CUI ; Xi PENG ; Min HE

Chinese Journal of Veterinary Science.2008;28(5):544-548.

By using routine H. E method and measuring the absolute weight and growth index of thymus and ANAE positive ratios, 1 to 49 days Tianfu duckling were selected to study the changing trends in the normal development of the thymus. The results are as follows:the thymus of duckling develops slowly from 1 to 14 days,and quickly from 21 to 35 days,then steadily from 35 to 49 days. By the 28th day,most T-lymphocytes have well differentiated and the thymus has reached its maturation. There are three types of thymie corpuscles whose functions have been discussed.

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Rhodococcus equi distribution in the soil environment of horses in Inner Mongolia,China——An investigation report

Zhuang DING ; Takai SHINJI ; Madarame HIROO ; Shuang CHANG ; Hainan HUANG ; Xiaowei HUO ; Minghua GAO ; Zhongtian TAN ; Shuangcheng GAO ; Hatori FUMIKO ; Sasaki YUKAKO ; Kakuda TSUTOMU ; Tsubaki SHIRO

Chinese Journal of Veterinary Science.2008;28(1):40-44.

Little is known about the distribution of Rhodococcus equi in the soil environment of native horses in China. One hundred and eight soil samples were collected from native-horse farms in the Hulun Beier grasslands of eastern Mongolia, the Xilin Goler grasslands of southern Mongolia, and Tongliao city in Inner Mongolia of China for investigating the distribution of R. equi in these regions. The isolation rates of R. equi from soil samples from the Hulun Beier and Xilin Goler grasslands ranged from 25.9% to 30.0%. In contrast, isolation rates from soil samples from Tongliao city was as high as 82.3% and the mean number of R. equi in soil samples from Tongliao city was 10 times more than those of samples from the grasslands. The 488 isolates were examined using PCR for the presence of genes that encode virulence-associated 15 000-17 000 antigen protein (VapA) and the 20 000 antigen protein (VapB). All isolates were negative for virulence-associated proteins. Plasmid profiles of these avirulent isolates showed that cryptic plasmids of various sizes were present with an incidence of 13.3% to 21.5%. The results of the present study contrast with those of our recent study, in which we reported that R. equi was absent from Mongolian horses in Ulaanbaatar, Mongolia. It is suggested that the difference between the results of these two studies is due to the mobile pasturing system in Mongolia and nonmobile pasturing system in Inner Mongolia.

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Effects of particulate montmorillonite on tissue lead levels, erythropoiesis,ALA-D activity,and lead-induced lipid peroxidation in liver of pigs

Dongyou YU ; Xiaogang YANG ; Zirong XU

Chinese Journal of Veterinary Science.2006;26(6):673-676.

Sixty barrows (Duroc × Labdrace × Yorkshine) were randomly assigned to two groups by weight of 33 kg,each of which was replicated three times with ten pigs. Half of the pigs were fed with diets containing 10 mg/kg lead and 0.5% particulate montmorillonite,the other half pigs were fed only with diets supplemented 10 mg/kg lead as control groups for 100 days.The results showed that the addition of particulate montmorillonite to the diet significantly decreased lead concentration in tissues such as blood,brain,liver,bone,kidney and hair and enhanced the erythropoiesis as measured by increasing numbers of RBC,hemoglobin and hematocrit values ,and elevated ALA-D activity in liver. The damage of lead to the liver was evident in the increases in hepatic concentration of malondialdehyde (+ 17.08% );decreases in the antioxidant enzymes catalase(-85.73 % ),superoxide dismutase ( - 52.17% ) and glutathione peroxidase ( - 47.56% ). Concomitant use of particulate montmorillonite in the diets completely ameliorated the lead-induced oxidative damage. It indicated that particulate montmorillonite is possessed of the potential therapeutic activity against lead poison.

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Effect of Zinc Toxicity on Peripheral Blood T-lymphocyte in the Broiler

Hengmin CUI ; Cuiyan ZHAO ; Xi PENG ; Junliang DENG ; Debing LI

Chinese Journal of Veterinary Science.2006;26(3):310-313.

The experiment was conducted to examine the effect of zinc toxicity on the peripheral blood T-lymphocyte by the method of flow cytometry(FCM) and ANAE.200 one-day-old Avian broilers were divided into four groups,and fed with the diets as follows:①control group(Zn 100 mg/kg diet) and ② zinc toxic groups(Zn 1 500 mg/kg diet,zinc toxic group Ⅰ;Zn 2 000 mg/kg diet,zinc toxic group Ⅱ;Zn 2 500 mg/kg diet,zinc toxic group Ⅲ) for seven weeks.The ANAE positive ratios of the peripheral blood T-lymphocytes were much lower in the three zinc toxic groups than that in control group at 7 weeks of age.CD4+ T cell numbers were reduced from 2 to 7 weekds of age in zinc toxic group Ⅲ and from 6 to 7 weeks of age in zinc toxic group Ⅱ,as compared with that of control group.The numbers of CD8+ T cell decreased at 2 and 4 weeks of age in zinc toxic groups Ⅱ and Ⅲ,and at 7 weeks of age in zinc grups Ⅰ and Ⅲ.CD4+/CD8+ ratio was higher at 2 and 4 weeks age,and lower at 6 and 7 weeks of age in zinc toxic groups Ⅰ,Ⅱ and Ⅲ than in control group.The results showed that zinc toxicity would suppress the development of T-lymphocytes and reduce the peripheral blood T-lymphocyte populations.Potential mechanism underlying these observations are also discussed.

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Effect of Copper Toxicity on Peripheral Blood T-lymphocyte in the Chicken

Hengmin CUI ; Guang YANG ; Xi PENG ; Junliang DENG ; Debing LI

Chinese Journal of Veterinary Science.2006;26(4):427-431.

The experiment was conducted to examine the effect of copper toxicity on the peripheral blood T-lymphocyte using the flow cytometry(FCM) and ANAE.180 one-day-old Avian broilers were divided randomly into three groups,and fed diets as follows:(1)Controls(Cu 11.97 mg/kg diet) and (2)copper toxic(Cu 650 mg/kg diet,copper toxic group Ⅰ;Cu 850 mg/kg diet,copper toxic group Ⅱ) for six weeks.The ANAE positive ratios of the peripheral blood T-lymphocytes were much lower in the two copper toxic groups than in control group from 1 to 6 weeks of age(P＜0.05 or P＜0.01).Also,there was significant difference between copper toxic groups Ⅰ and Ⅱ at 1,3,5 and 6 weeks of age(P＜0.05 or P＜0.01).CD+4 T cell numbers reduced from 2 to 6 weeks of age in both copper toxic group Ⅰ and copper toxic group Ⅱ as compared with those of control group(P＜0.05 or P＜0.01).At the same time,there was significant difference between copper toxic groups Ⅰ and Ⅱ at 6 weeks of age(P＜0.05).But the numbers of CD+8 T cell were not varied from 2 to 6 weeks of age in copper toxic groups Ⅰ and Ⅱ in comparison with those of control group(P＞0.05).The CD+4/CD+8 ratio was lower from 2 to 6 weeks of age in copper toxic groups Ⅰ and Ⅱ than in control group.The results showed that copper toxicity could suppress the development of T-lymphocytes and reduce the peripheral blood T-lymphocyte populations.Potential mechanisms underlying these observations are also discussed.

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Synthesis and Identification of the Antigens for Ciprofloxacin

Yu ZHOU ; Yansong LI ; Zhe WANG ; Jianhua TAN ; Zengshan LIU

Chinese Journal of Veterinary Science.2006;26(2):200-203.

Hapten-carrier protein conjugates were made using ciprofloxacin (CPFX) and two carrier proteins by 1-ethyl-3-(3- dimethylaminopropyl)-carbodiimide hydrochloride (EDC) method. Ultraviolet spectrophotometry were used to demonstrated that the molecule conjugate ratio of CPFX to ovalbumin (OVA) and bovine serum albumin (BSA) are 6:1 and 13:1 respectively. Nondenaturing gel electrophoresis results revealed that the conjugate band migrates differently from that of the carrier protein alone and of the EDC-treated protein when as few as 6 molecules of CPFX are attached to the carrier protein. The results indicate that nondenaturing gel electrophoresis and ultraviolet spectrophotometry can be employed to analyze the molecule coupling ratio of CPFX to carrier proteins qualitatively and quantitatively.

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Antibiotic Sensitivity Detection and Analysis of Escherichia coli Isolated Clinically in China

Liancheng LEI ; Dan ZHENG ; Wenyu HAN ; Fengtao JI ; Weidong XU

Chinese Journal of Veterinary Science.2005;25(5):470-473.

The susceptibility to 9 kinds of antibiotics of 393 animal pathogenic E. coli isolated from clinical samples was determined from 2000 to 2003. The resistance to TC, GM, CMP, AMP, RA, KM, FT, SM and CIP were 93.89%, 57.76%,78.63%, 77.86%, 92.11% ,47.33%, 46.82%, 76.84% and 74.81%, respectively. The isolates could be classified into eight classes according to the number of drugs to which srains were resistant. The resistance spectrum of the isolates varied from 2to 9 kinds of the above drugs. The strains that were resistant to seven kinds of drugs were more than 80 percent. The resistance rates of swine origin strains to GM,AMP and KM were higher than those of poultry origin strains, while the resistance rates of poultry origin strains to TC, CMP, SM and CIP were higher remarkably than those of swine origins. The frequency of resistance increased from 2000 to 2003,which was from 67.33% to 90.58% for CMP, 71.29% to 84.81% for AMP, 73.76%to 80.10% for SM and 61.88% to 88.48% for CIP. At the same time, the resistance rate of GM decreased from 61.39% to 53.92%. Thirty-seven strains (95 %) could make all the Kunming mice die within 72 h injected intraperitoneally with the culmice three times in vivo. The pathogenicity of wild strains with drug resistance acquired naturaly to mice did not decline.

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