Chinese Journal of Veterinary Science 2009;29(5):643-648

Effect of disequilibrium of calcium homeostasis on apoptosis of MDCC MSB1 cells induced by lanthanum chloride

Lan ZHAO ; Wenjun LI ; Jintao WANG ; Shiwen XU

Keywords

lanthanum chloride; MDCC-MSB1; DNA damage; alcium homeostasis; apoptosis

Country

China

Language

Chinese

Abstract

To investigate the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride(LaCl3 ), MDCC-MSB1 cells was conventionally cultured in RPMI1640, which was tured for 24 h, MTT was utilized to detect the cell multiplication inhibition ratio,DNA ladder and TUNEL to detect apoptosis,the Fura-2/AM as the probe to detect the [Ca2+]i within cells. The results indicated when LaCl3 concen and [Ca2+]i also increased,and presented a dose-effectiveness relationship. LaCl3 could restrain the proliferation of MDCC-MSB1 cells,suggesting their apoptosis induced by changing [Ca2+]i.