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Establishment of multiplex PCR assay for detection of HIV-1

Lili DAI ; Dexi CHEN ; Ying SHI ; Feili WEI ; Bo SHENG ; Yasong WU ; Yali LIU ; Honghai ZHANG ; Lianchun LIANG ; Tong ZHANG ; Hao WU

Chinese Journal of Laboratory Medicine.2009;32(8):866-872. doi:10.3760/cma.j.issn.1009-9158.2009.08.006

reproducible, and may cover the major circulating strains in China.

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Development of a rapid triplex TaqMan real-time PCR assay for the detection of toxigenic Vibrio cholerae

Hui YANG ; Cheng GONG ; Lijuan WU ; Jihong HU ; Jianping CAI

Chinese Journal of Laboratory Medicine.2009;32(9):1038-1043. doi:10.3760/cma.j.issn.1009-9158.2009.09.017

for the detection of their virulent abilities simultaneously.

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Comparative study of different screening markers for hemodialysis patients infected with hepatitis C virus

Bin LOU ; Yajun TAN ; Jian FAN ; Jie ZHANG ; Yu CHEN

Chinese Journal of Laboratory Medicine.2009;32(9):993-996. doi:10.3760/cma.j.issn.1009-9158.2009.09.007

ssay provides a higher detection rate for HCV infection in HD patients.

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Distribution and antimicrobial resistance change of blood culture isolates from the year 2004 to 2007

Sing GU ; Shiyang PAN ; Xuefei WEI ; Wenying XIA ; Yi WEN ; Yaning MEI ; Mingqing TONG

Chinese Journal of Laboratory Medicine.2009;32(8):889-894. doi:10.3760/cma.j.issn.1009-9158.2009.08.012

n positive blood culture, and they are resistant to a variety of antimicrobial agents, which should be called attention.

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Multiplex RT-PCR assay for the detection of 8 kinds of respiratory viruses

De WU ; Yanling MO ; Lirong ZOU ; Hui LI ; Qiuxia CHEN ; Ling FANG ; Ping HUANG ; Changwen KE

Chinese Journal of Laboratory Medicine.2009;32(8):861-865. doi:10.3760/cma.j.issn.1009-9158.2009.08.005

. Conclusions Two multiplex RT-PCR assays show high consistency with common RT-PCR. The multiplex RT-PCR assays were initially established.

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A novel base-quenched probe technique for detecting single-nucleotide polymorphisms

Jun ZHANG ; Guanghua LUO ; Lu ZHENG ; Xiaoying ZHANG ; Ning XU

Chinese Journal of Laboratory Medicine.2009;32(9):1064-1068. doi:10.3760/cma.j.issn.1009-9158.2009.09.025

ncing analysis validated that all four-type base-quenched probes could provide unbiased genotyping results ( Kappa =1, P=0.00), although. Conclusion This method is simple, economic and suitable for large-scale genotyping studies.

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Some key issues in immunologica; surveillance and its clinical application

Renqian ZHONG

Chinese Journal of Laboratory Medicine.2009;32(7):735-738. doi:10.3760/cma.j.issn.1009-9158.2009.07.003

Immunological surveillance, analysis including cells to biological is one of the important components in clinical laboratory immunology. This paper focuses on three issues. First of all is the measurement of antigen-specific T cell, which is very important for understanding the pathogenesis of immunological related diseases because of the central role played by T cell in immune response. It is optional to adopt different assays according to different facilities and puroposes such as flow cytometer or the number or function of T cells. Secondly, it is no doubt that positive autoantibodies are key bio-markera in the diagnosis of autoimmune diseases. And what is more autoantibodies could predict the occurrence of certain autoimmune diseases. More and more researches showed that autoantibodies could be detected in patients with tumors, which help for either diagnosis of tumors or screening of therapeutic target markers. Finally, the application of cytokine measurement is discussed. Clarification of cytokine profiles is the way to understanding pathogenesis of immunological related diseases, it is also in favor of monitoring severity of the diseases, But there are shortages concerning organ or tissue specific, as well as good quality control.

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Analysis,identification and strategies on interferences in immunoassay

Gusheng TANG ; Yu WU ; Qian SHEN

Chinese Journal of Laboratory Medicine.2009;32(7):725-729. doi:10.3760/cma.j.issn.1009-9158.2009.07.001

Substances that might potentially alter the measurable concentration of the analyte or alter the binding ability of detection antibody can lead to immunoassay interference. Endogenous interferences consist of autoantibodies, heterophiles antibodies, human anti-animal antibodies (HAAA) and some binding proteins. Lipidemia, cross-reactivity, pre-analytical variation, matrix and different detection equipments may also affect immunoassay. These interfering substances may cause falsely increased or decreased concentration in many analytes, including hormones, tumor markers, drugs, cardiac tropanin and microbial serology, thus it will affect the diagnosis of patients and the evaluation of therapeutic strategies Laboratorians and physicians should both be aware of the potential interference in immunoassays and communicate closely when any clinical discordance between the clinical and the laboratory data appears, avoiding a subsequent wrong diagnosis and unwarranted treatment. In this case, an alternative assay or measurement is needed for the potential correct results.

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Determination of autoantibody profile in patients with primary biliary cirrhosis and its clinical appfication

Yang ZHANG ; Yongzhe LI ; Xue FENG ; Shumeng BAO ; Chaojun HU ; Dawei TONG ; Shulan ZHANG ; Fengchun ZHANG

Chinese Journal of Laboratory Medicine.2009;32(7):760-763. doi:10.3760/cma.j.issn.1009-9158.2009.07.008

Objective To detect the value of autoantibedy profile in primary biliary cirrhosis (PBC). Methods 96 serum samples of patients with primiary biliary cirrhosis, 100 serum samples of other antoimmune disease and 49 serum samples of healthy were tested for anti- M2, anti-3E(BPO), anti-Sp100,anti-PML,anti-gp210,anti-LKM-1 ,anti-LC-1 ,anti-SLA/LP by EUROLine. Results The positive of the anti-M2,anti-BPO, anti-Sp100, anti-PML and anti-gp210 for PBC was 76. 0%, 84.4%, 32. 3%, 28. 1% and 35.4% ,respectively. The positive of the anti-M2, anti-BPO, anti-Sp100, anti-PML and anti-gp210 for other autoimmune disease was 13.0% ,9. 0% ,3.0% ,2.0% and 1. 0%, respectively. The sensitivity of the anti-M2 for PBC was 76. 0% ,with specificity of 87. 0%. The sensitivity of the anti-BPO for PBC was 84. 4%, with specificity of 91.0% ;the sensitivity of the anti-Sp100 for PBC was 32. 3%, with specificity of 97.0%. The sensitivity of the anti-PML for PBC was 28. 1% ,with specificity of 98.0%. The sensitivity of the anti-gp210 for PBC was 35.4%, with specificity of 99. 0% . Anti-LKM-1, anti-LC-1, anti-SLA/LP positive patients with PBC were not detected;the incidence rate of liver function failure in anti-gp210 positive serum higher than anti-gp210 negative serum (χ2 = 11.17, P < 0. 01). Conclusions Multiple autoantibedies can be detected in the sera of PBC patients. The detection of autoantibody profile is useful for the diagnosis and differential diagnosis of PBC, and may he helpful for therapy and prognosis of PBC.

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Negative interference of circulating troponin autoantibodies in five commonly used cardiac troponin Ⅰ detection systems

Yu WU ; Weiguo ZHAO ; Gusheng TANG ; Qian SHEN

Chinese Journal of Laboratory Medicine.2009;32(7):749-753. doi:10.3760/cma.j.issn.1009-9158.2009.07.006

Objective To explore the possible negative interference of circulating cardiac troponin Ⅰ(cTnI) autoantibody on the immunoassay of cTnI in five commonly used cTnI detection systems. Methods Thirteen patients with positive cTnI autoantibodies in their serum samples were firstly screened and selected from 121acute myocardial infarction (AMI) patients using ELISA assay. The serum cTnI values and their recovery rates were then carefully measured and analyzed. Results cTnI values in these 13 samples showed amazing difference in the five detection systems, demonstrating various degrees of pseudo-drop, or even false-negative. One sample with low recovery was detected in Access-2 system. One sample with low recovery as well as one sample with moderate recovery were detected in Architect i2000 (Abbott). Two samples with moderate recovery and one sample with low recovery were detected in Axsym(Abbott). Three samples with moderate recovery and two samples with low recovery were detected in Dimension X Pand (Dade Behring)and one sample with moderate recovery together with four samples with low recovery were detected in Vidas (Biomerieux). And the serum levels of autoantibodies (A450) positively correlated with the degrees of their negative interference for the detection of cTnI. The R2 and P values on each system were 0. 841 (P <0. 01)vs Access-2, 0. 808 (P < 0. 01) vs Architect i2000 (Abbott), 0. 772 (P < 0. 01) vs Axsym (Abbott), 0. 707 (P < 0. 01) vs Dimension X Pand (Dade Behring) and 0. 424 (P < 0. 05) vs Vidas (Biomerieux), respectively. Conclusion Circulating autoantibodies of cTnI can induce considerable negative interference in all the 5 commonly used cTnI detection systems, which might then lead to incorrect judgments of the obtained results of cTnI in daily clinical work.

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