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STUDIES ON ANTAGONISTIC INTERACTION OF HUMAN SKIN NORMAL FLORA AND COMMON PATHOGENIC BACTERIA

Microbiology.1992;0(04):-.

Biological antagonism was found to be only one aspeet When we Study the interactions among the microbial populations in and on the human beings. We examined the following strains of the resident flora isolated from the human healthy skin, including propionibacterium acnes (A14-1), Staphylococcus epidermidis (F65), and a number of the common dermal pathogenic micro-organisms such as staphylococcus aurcns. Ps. acruginosa snd E. coli, for their antagonistic interactions in vitro. The following results were obtained, they revealed obvious antagonism After 48h of contact in vitro antagonistic interaction to appear, and after 72h antagonism displayed obviously. On the other hand, P. acnes (A14-1) and S. epidermidis (F65) showed no antagonisim, they behaved commesaly, such commensality seemed to be important for the autoperification of the human normal skin surfaces resulting in dermal microeubiosis of the human beings.We examined the members of the transient microbial flora of the healthy human skin Lactobacterium (ad3), Bifidobacterium (Bf1) and some common pathogenic bacteria in antagonistic experiments in vitro, and discovered that they showed micropopulations appeared to be of great value to the maintenance of good health to their host.

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Cloning and Expression of Alpha-Toxin Gene from Clostridium perfringens Type C

Chongbo XU

Microbiology.1992;0(01):-.

Alpha-toxin gene was amplified from chromosomal DNA of Clostridium perfringens type C by polymerase chain reaction(PCR). PCR product was inserted into vector pGEM-T directively. The cloned recombinant plasmid pXCPA1 possesses positive nucleotide sequence of alpha-toxin. A 1.2 kb alpha-toxin gene fragment was cleaved with restriction endonucleases NcoI/EcoRI from plasmid pXCPA1, and then inserted into an expression vector pET-28c which cleaved with NcoI/EcoRI by blunt-end ligation. The recombinant expression plasmid pETXA1 was studied in detail by restriction endonucleases analysis and nucleotide sequencing. The results showed that the recombinant expression pETXA1 possessed a positive alpha-toxin gene sequence and reading frame. BL21(DE3) (pETXA1) could produce alpha-toxin and the expressed products were recognized by alpha-toxin monoclonal antibodies, and the expression level of the alpha-toxin proteins were about 16.28% of total cellular protein by SDS-PAGE and thin-layer gel scanning analysis.

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Relationship between Normal Bacteria Flora and Human Body

Zhiyi XU

Microbiology.1992;0(03):-.

Close relationship exists between normal bacteria flora in intestinal tract of human body with health. Health and outside enviroment conditions must maintain relative equilibrium to keep body healthy.Certain factors might destroy such equilibrium resulting the occurrence of some diseases. Following are general discussion of normal bacteria flora,their relationship with health and how to adjust dysbacteria into normal.

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Potential of Co-culture Induction in Antibiotics Discovery from Bacteria-associated with Marine Sponge Hymeniacidon perleve

Pengfei GUO

Microbiology.1992;0(01):-.

Ten actinomycetes strains from inter-tidal marine sponge Hymeniacidon perleve of China Yellow Sea were isolated and bio-assayed for antibiotic activities.3 of the 10 strains displayed antimicrobial activity when in monoculture.While strain Hp-053 was co-cultured with each of the other 9 strains,5 of the 9 co-cultures exhibited enhancing antimicrobial activity.TLC results and bio-autography of extracted samples indicated that chemical compounds and antimicrobial compounds produced by co-cultures of sponge associated actinomycetes were different from those by monoculture.Co-culture induction may be a valuable novel tool for the exploitation of sponge associated microorganism.

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Expression of VP2 Gene of Feline Panleukopenia Virus Isolated from Tiger in Pichia pastoris Yeast

Songtao YANG

Microbiology.1992;0(01):-.

In this study,the VP2 gene of feline panleukopenia virus(FPV) isolated from tiger was successfully cloned and expressed in Pichia pastoris yeast.The VP2 gene was amplified by PCR with a pair of specific primer.Then PCR products was purified and cloned into pGEM-T for sequencing.The interesting gene fragment was recovered after the double enzyme digestion of EcoRI/NotI,then subcloned into pPICZ?A for secretory expression.The recombinant pPICZ?AVP2 was linearized with SacI and then transformed into competence yeast GS115 for expression under the induction of 1% methanol.The positive recombinants were screened by PCR method.The expression product was identified by SDS-PAGE and western-blotting.The results showed that there was a molecular weight of 32 kD,which could be specifically recognized by polyclonic antibody against FPV.It revealed that the recombinant VP2 protein had correct three-dimensional structure,which would be used as a antigen protein for the diagnosis and prevention of FPV infection in tigers.

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Screening of Ganoderma Strain of High Yield Intracellular Triterpenes and Optimization of Its Fermentation Conditions

Suping YU

Microbiology.1992;0(01):-.

According to the yield of intracellular triterpene, the strain GL31 was screened out from 22 strains of Ganoderma collected from various regions. The optimal fermentation conditions were studied by single factor experiments and orthogonal test. The optimal parameters of carbon source, nitrogen source, initial pH, the medium volume in the flask and cell density were obtained. In addition, the metabolic curves of intracellular triterpene and biomass were determined. It was found that the yield of intracellular triterpenes was up to 3.51?10~(-2)g/100 mL medium at the 84th hour. In addition the stram was staticly fermented 144 hours flowing 84hours shaking-flask culture, the IT (intracellular triterpenes) content was increased by 48.6% and the yield of IT was increased by 65% compared with those of the mycelia cultured 84hours using shaking-flask method. This indicated that static fermentation method is helpful for improving the total IT content. The result also show that the highest yield of intracellular triterpenes of mycelia staticly fermented flowing 84hours shaking-flask culture is higher than that of mycelia using shaking flask culture method.

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Advances on Metabolic Engineering of Fuel Ethanol

Rong YOU

Microbiology.1992;0(03):-.

Ethanol is a renewable liquid fuel which has the most potential future. It is mostly produced by biofermentation. Many scientists pay attention to change ethanol metabolic network and increase ethanol productivity using the technology of metabolic engineering. This paper roundly illustrates that metabolic engineering is applied on fuel ethanol production from such as like extension of substrate use and design of new metabolic pathways.

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Teaching Reform and New Practices for the Food Fermentation Technology Curriculum

Lei ZHAO

Microbiology.2008;0(09):-.

A new teaching model for food fermentation technology education is proposed in this paper,aiming at improving some of the drawbacks of the traditional teaching method. Some novel approaches,such as summarization,comparison,discussion,as well as bi-lingual lecturing and multi-media assisted teaching,are introduced to inspire students’ passion for learning. Meanwhile,arranging students as dedicated class speaker and organizing students to participate in the exploring experiments also reflect the technology major education characteristics of normal university.

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Prevention and control of Multidrug Resistant Tuberculosis

Bin YI

Microbiology.1992;0(06):-.

On recently years,the tuberculosis shows the characteristic of higher incidence、 infection、drug-resistance、and Morbidity。However,the emphasis and difficult is preventing and controling of multidrug resistant tuberculosis.Its nosocomial and societal causes of occuring and prevailing were analyzed and control measures from seven facets were expounded in this article.Those include prevention knowledge widely,strengthen management and supervision of floating population,improve ability of diagnosis and therepy,develop combinasion treatment of traditional chinese medicine and western medicine and other assistant treatment, prevent community and nosocomial infection,perfect the drug-resistant surveillance system,carry out the study of new drug 、measure and treatment to anti-tuberculosis actively.

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Feasibility Study on Earthworm Feed Directly on Excess Sludge

Chunjie BAI

Microbiology.1992;0(01):-.

Raising earthworms in boxes with excess sludge is conducted.It is concluded that excess sludge is eatable to earthworms ,and feeding condition is≤30cm filling thickness with fresh excess sludge each time,≤20cm up-filling thickness with rotten-lumpy excess sludge each time.

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