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INTERACTION OF ASSOCIATIVE NITROGEN-FIXATION BACTERIA WITH EVCAIYPTUS

Microbiology.1992;0(04):-.

The interaction of Klebsiella mytoca NG13/pMC73A with Eucalyptus were studied. The results showed that the root surface and even the inner cortex of Eucalyptus were colonized by K. oxytoca. The K. oxytoca was reisolated from rhizosphere, root surface and inner root of inoculated Eucalyptus. The inoculation with K. oxytoca stimulated the excretion of Eucalyptus root and affected the contents of amino acids, carbohydrates, phytohormones of root exudates. The seedlings of Eucalyptusto to inoculation with K. oxytoca increased growth, total dry matter and N content by 29.81% - 100.40% after 3 months in comparinson to the uninoculated seedlings.

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Study on Fermentation Kinetics of Nisin by Lactococcus lactis

Xuliang ZHUANG

Microbiology.1992;0(03):-.

The kinetics of growth, lactic acid and nisin production and substrate consumption by Lactococcus lactis subsp. lactis SM526 were studied in different pH. Growth, lactic acid and nisin production were modelled using a simple logistic equation while substrate consumption was foud to be a function of growth and lactic acid production rate. The optimal pH for growth and lactic acid production was 7.0 while it was 6.5 for nisin production according to the models developed.

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Affinity Selection of Human ScFv Against Botulinum Neurotoxin Serotype A

Hui WANG

Microbiology.1992;0(01):-.

In this work, rare specific binders to botulinum neurotoxin antigen were selected from human phage immunized library through three rounds of panning in vitro. Of them, clone ScFvB17 is of high affinity and specificity to target antigen. B17 is of 750bp encoding 250 amino acid. Analysis results of gene structure showed V-genes of B17 belonging respectively to VH4 or? chainⅡ family are new antibody Fv genes. More important result showed that recombinant ScFvB17 expressed in E.coli could bind specific for neurotoxin competing with antiserum. Successful selection of human phage antibody library and obtaining specific ScFv against botulinum neurotoxin serotype A provide useful materials for further study on detection of neurotoxin and human engineering therapeutic antitoxin.

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CLONING OF GENES ESSENTIAL FOR XANTHAN POLYSACCHARIDE BIOSYNTHESIS FROM XANTHOMONAS CAMPESTRIS NK-01

Shiyan LI

Microbiology.1992;0(05):-.

Xanthomonas campestris NK-01 produces amounts of xanthan polysaccharide. Nonmucoid mutants, defective in synthesis of xanthan polysaccharide, were isolate after treatment with ethylmethanesulfonate. To isolate genes essential for xanthan polysaccharide synthesis complete libraries of DNA fragments from wild-type Xanthomonas campestris NK-01 were constructed in E. coli HB101. The pooled clone bank was conjugated en masse from E. coli into a nonmucoid mutant. Kanamycin-resistant exconjugants were then screened for the ability to form mucoid colonies. Analysis plasmids form muciod exconjugants indicated that overlapping segments of DNA had been cloned. These plasmids were tested for complementation of nine additional nonmucoid mutants. A 13. 4 kb region of DNA was determined to contain at least some genes essential for xanthan polysacchdride synthesis.

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Cloning and Squence Analysis of Gene Coding for Dextransucrase from Leuconostoc mesenteroides

Yanchun SHAO

Microbiology.1992;0(03):-.

The desired DNA product of dsr1 and dsr2 was amplified from the total DNA of the L0309 screened in this lab by PCR with two pairs of gene specific primers.The segment of dsr1 and dsr2 was inserted into pUC19 vector and integrated into the entire gene of dsrx.The result of restriction endonuclease mapping and sequencing shows that 4566 bp of dsrx covers the entire open reading frame,encoding 1522 amino acid residues.There is an identity of 99% between the gene of U81374 and dsrx about their nuclear acid sequences.The homology of the deduced amino acid level amount to 98.49% with that of U81374.

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USING SNAIL ENZYMES TO EXTRACT AND PURIFY THE DNA OF CRYPTOCOCCUS

Zhiqiang ZHAO

Microbiology.1992;0(01):-.

This article introduced a method to extrct and purify the DNA of Cryptococcus. Protoplasts were obtained after digested by snail enzyme. We washed them with SE liquid again and again to get rid of the capsular polysaccharides and split them with thermal treatment of 55 degrees centigrade. Then, satisfactory results of the extrction and the purification were achieved. From one wet gram of the yeast, more than 600 ?g DNA can be acquired. The DNA gained is especially suited for the determination of G+C mol% content by use of the T_m method.

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REPORT ON CULTURE CONSERVATION OF ACTINOMYCES AND MOLDS

Shankang FANG

Microbiology.1992;0(06):-.

The sand tube method for the preservation of 244 strains of Actinomyces at room temperature through a period of 29 years has been tested. The survival rates (%) of these strains were 96 for 6 yrs; 91 for 9yrs; 88 for 11yrs; 78 for 14yrs; 76 for 15yrs; 74 for 17yrs; 70 for 20yrs; 59 for 22 yrs ; 47 for 25 yrs ; 40 for 27 and 37 for 29 yrs . The antimicrobiai activities and antibiograms of existing strains were identical with their original strains. The conservation of 12 genera. 216 strains of molds at room temperature for 16 yrs was carried out by moldy bran method. Their survival rates varied from 0 to 100% depending on their species. All of them deceased at 16th years.

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A STUDY ON ULTRA LOW TEMPERATURE FREEZING OF ALKALOPHILIC MICROORGANISMS

Yuguang ZHOU

Microbiology.1992;0(01):-.

In this paper, response of the 7 bacterial strains belonging to alkalophilic microorganisms subjected to liquid nitrogen freezing and thawing was reported. According to the cell survival rate, the protective efficiency of 10% glycerol and 5% DMSO was equal to the protective efficency of the same method used in general bacterial cultures after preservation at frozen state for 3 months. The cell survival rate of alkalophilic microoraganisms should be increased if using alkaline protective agent.

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FINDING OF BDELLOVIBRIO BACTERIOVORUS INFACTING RHIZOBIUM JAPON ICUM

Heng XU

Microbiology.1992;0(01):-.

Four bdellovibrio bacteriovorus of Rhizobium japonicum were isolated in our country from field soils cropped to soybeans. The culture character, morphology, host range and the other biological properties of these bdellovibrio bacteriovorus of R. japonicum had a wide host range and a strong ability that lyse R. japonicum in field soils, and were one of main biological factors that lower the yield of soybeans.

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STUDY ON THE INHIBITION PHAGE FROM DIAD POSITIVE ION Ca~(2+), Fe~(2+) IN THE GLUTAMATE FERMENTATION

Zhilan LING

Microbiology.1992;0(02):-.

Using Ca~(2+), Fe~(2+) in proper concentration as the inhibitor of phage 530, the strains T_6-13 and B_9 of L-Glutamate-producing bacteria could not be lysised. These strains produced the Glutamate no less than the parental strain did.

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