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THE STUDY OF RELATIONSHIP BETWEEN THE MOVEMENT OF SOIL BACTERIAL NUMBER AND ECOLOGICAL FACTORS ON THE LEYMUS CHINENSIS GRASSLAND

Chongbang ZHANG

Microbiology.1992;0(02):-.

This paper preliminary studied the movement of soil bacterial number, and the relationship with 8 kinds of ecological factors. The result indicated: soil bacterial number only appears a biggest value in one year (2. 67?10-7个 individual g-1 dry soil). Among 8 kinds of ecological factors, soil water content, temperature, hydrolyted nitrogen, quick acting postossium and active organic matter have straight relationship with siol bacteria, and have negative relationship with soil pH, electric conduct rate, effective phosphorus. The component analysis indicated that soil water content, temperature, electric conduct rate, active organic matter are principal effective factors.

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IMMOBILIZATION STUDIES OF PENICILLIN ACYLASE ON THE POROUS BEAD WITH OXIRANE GROUPS

Haijun XIAN

Microbiology.1992;0(03):-.

Penicillin Acylase from B. megaterium was immobilized on the porous bead carriers based on methacrylate, N,N-methelene-bis-methacrymide, glycidyl methacrylate, Allyl ether copolymers (Eupergit-c) either directly or after chemical modification with 1.6-deaminohexane and L-Lysine. Directly binding with oxirane groups, the most efficient immobilization results were achieved. The immobilization yield was markedly influenced by the ratio of amount of free enzyme to the weight of the carrier. The specific activities of 89 up to 475IU/g (wet) and binding protein of 6.9 to 112 mg/g (dry) were obtained when the free enzyme added to the immobilization solution was from 323IU/g (dry) up to 6250IU/g (dry). The residual activity of immobilized PGA in a recycling system at the 20th was about 92.5% of the initial value.

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ASSOCIATION OF ABO BLOOD GROUP WITH HELICOBACTER PYLORI INFECTION AND ITS SIGNIFICANCE

Yong XIE ; Chongwen WANG ; Jinquan ZHU ; Kunhe ZHANG ; Meizhen ZHOU

Microbiology.1992;0(06):-.

The Helicobacler pylori and ABO blood group of 217 patients and 169 blood donorswere identified by bacteriologic and serologic method,Aiming to expore the relation between H. pylori infection and ABO blood group. The result show: (1) Among patients with peptic ulcer there were a increase in the proportion of blood group O; (2) The infection rate of H.pylori in the proportion of bloodgroup O was significatly higher than in those not blood group O (P

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EFFECT OF MICROENVIRENMENT ON LIPASE-CATALYZED ENANTIOSELECTIVE ESTERIFICATION OF KETOPROFEN

Wei DU ; Minhua ZONG ; Rong YANG ; Qiong LI ; Yong GUO

Microbiology.1992;0(05):-.

The effect of reaction media cosolvent water activity, temperature and pH on Novozym 435-caulyzed enantioselective esterification of ketoprofen was systematically explored. Novozym 435 showed high catalytic activity and enantioselectivity in cyclohexane; E value increases markedly by addition of toluene to cyclohexane; the optimum temperature and the initial water activity were found to be 30℃ and 0.09 respectively; pH shows little effect on enzymatic reaction wilson the scope studied.

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IDENTIFICATION OF PLANT PATHOGENIC BACTERIUM FROM CROWNGALL OF JAPANESE CHERRY (PRUNUS XYROLOEUSIS) ANDCONTROL TECHNOLOGY

Dawei NI ; Jie SHENG

Microbiology.1992;0(01):-.

11 plant pathogenic strains were isolated from the crown gall of Japanese cherry (Prunus xyroloensis)in Japanese cherry growing areas, such as Chixing. fenhua and Shenzhou counties of Zhejiang province. Ten ofthem were identified as Agrobacterium tumefaciens biotype 1 based on their morphological. physiological andbiochemical characterizations and bacterial protein SDS-PAGE test. The plate antagonistic assay and the potbiocontrol test indicated that the isolated strains of A tumefaciens biotype 1 were sensitive to the well-knownantagonistic bacterium , K84 strain. The K84 strain could inhabit the tumor production by the pathogenic satins.

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THE EFFECT OF INHIBITOR VARIATIONS ON THE GROWTH OF BACILLUS LICHENIFORMIS

Tianhu LIU ; Qin SHANG

Microbiology.1992;0(05):-.

This paper reports reaction of Bacillus lichendermis on the inhibitor vacations. The result Shows that, inhibition of the 500?g/mL of cephalosporin 1000?g/mL of penicillin, 500?g/mL of streptimycin, on the Bacillus Licheniformis,Whe the Phis 7.0. This provides a sicentific basis for using the preparation of Bacillus licheniformis.

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SELECTION AND IDENTIFICATION OF NITRATE NON-UTILIZING MUTANTS OF COLLECTOTRICHUM GLOEOSPORIOIDES

Hong FENG ; Yizheng ZHANG

Microbiology.1992;0(05):-.

Eight isolates of Colletotrichum gloeosporioides isolated from host plants Cunninghamia lanceolata lanceolata and Euonymus japonichum, respectively, were cultured on MMC medium containing KCIO3 to select chlorate-resistant and nitrate non-utilizing mutants (Nit). All the Nit mutants obtained by this way belong to one of 3 kinds of the following phenotypes: the nitrate reductase structural locus (nit1), the nitrate-assimilation pathway-specific regulatory locus(nit3), and the molybdenum-containing cofactor locus(nitM). The higher mutation frequency on MMC amended with increasing concentration of KCIO3 was induced, and various nitrogen sources were able to influence production of the phenotypic classes. Seven of the 8 isolates tested were self-compatibility in which the two mutants with different phenotypes from the same isolate could genetically complement. The two isolates frail C. lanceolata belong to the same vegetative compatibility group (VCG), the other six isolates belong to distinctive VCGs.

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ASSAY OF EXTRACELLULAR PROTEASES OF AEROMONAS HYDROPHILA BY SKIM MILK PROTEOLISIS AGAR DIFFUSION TEST

Jieqing ZHU ; Chuanyi JI ; Chengping LU

Microbiology.1992;0(04):-.

A new method skim milk proteolysis ager diffusion test (SMPAD) was established. Extracelluar proteases in supernatants produced by Aeromonas hydrophila J-l in different mediums were detected by SMPAD and the Azocasein substrat method. It was demonstrated that the two methods agreed with each other and the SMPAD test was charactered by more easily operated, more sensitive and better repeated.

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A METHOD FOR ISOLATION AND PURIFICATION OF THERMOPHILIC CELLULOLYTIC ANAEROBES

Ruyang HAN ; Meici CHEN ; Yuhua ZHAO ; Hang MIN ; Xiaohang MA

Microbiology.1992;0(05):-.

A new method based on adherence of cellulolytic bacteria to insoluble cellulose for isolation and purification of thermophilic cellulolytic anaerobes was reported, in which Hungate anaerobic operating techniques were used to roll tubes with insoluble cellulose powder as substrate.

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A TECHNIQUE FOR DETECTION OF PLANT VIRUSES ──TISSUE BLOTTING

Mingquan XU ; Ping ZHENG ; Rongwei LIU ; Qing LIU ; Tao WANG

Microbiology.1992;0(05):-.

Tissue blotting, based on Enzyme-Linked Immunosorbent Assay (ELISA), is a technique for detection of plant viruses. This technique is not only high sensitivity and specificity, but also simpler and more rapid for detection. Samples that are blotted on membrane can be kept over three months. The results can directly display the section of virus infected. It is especially suitable for detection of plant viruses on a large scale.

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