Microbiology 1992;0(01):-

USING SNAIL ENZYMES TO EXTRACT AND PURIFY THE DNA OF CRYPTOCOCCUS

Zhiqiang ZHAO

Keywords

Cryptococcus; DNA; Extraction; Snail enzyme

Country

China

Language

Chinese

Abstract

This article introduced a method to extrct and purify the DNA of Cryptococcus. Protoplasts were obtained after digested by snail enzyme. We washed them with SE liquid again and again to get rid of the capsular polysaccharides and split them with thermal treatment of 55 degrees centigrade. Then, satisfactory results of the extrction and the purification were achieved. From one wet gram of the yeast, more than 600 ?g DNA can be acquired. The DNA gained is especially suited for the determination of G+C mol% content by use of the T_m method.