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The protective effect of high expression of mouse peroxisome proliferator activated receptor γ1 on free fatty acid induced β cell impairment

Yunfeng DU ; Liyong YANG

Chinese Journal of Diabetes.2005;13(4):296-298,301.

Objective To observe the protective effect of high expression of mouse proxisome proliferator activated receptor γ1（PPARγ1）on free fatty acid （FFA）-induced βTC3 cell impairment. Methods The recombinant plasmid pcDNA3.1/PPARγ1 was generated with cloning and was stably transfected into pancreatic β TC3 cells. The expression was detected with semi-quantitative RT-PCR. Then the cell viability of wild βTC3 cells was compared with that of the βTC3 cells with high expressed PPARγ1 by MTT viability assay after they were exposed to high-level FFA for 48 hours. Results The sequencing results for amplified target gene showed that the sequence of PPARγ1 from Chinese Kunming mouse is similar to that of mouse PPARγ1 in Genebank, only the codon coding Asp at the site of 421 amino acid changed from AAU to AAC. PPARγ1 was efficiently expressed in βTC3 cells in vitro. The cell viability of wild βTC3 cells reduced after being exposed to high-level FFA for 48 hours（P< 0.01）. Higher the level of FFA was, more obvious the reduction of the cell viability was （r=-0.962, P<0.01）. However, at the same condition, the cell viability of the βTC3 cells high expressing PPARγ1 had no significant change（P>0.05）. Conclusion The high expression of PPARγ1 could protect βTC3 cells from FFA-induced impairment

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Effect of losartan on urinary albumin excretion in experimental diabetic rats

Dongmei ZHANG ; Huiju ZHONG ; Min ZHOU

Chinese Journal of Diabetes.2000;8(6):360-361,359.

Objective　To observe the effect of losartan on urinary albumin excretion in streptozotocin diabetic rats.Methods　The following groups of rats were studied:normal control rats(NC),diabetic control rats (DC),diabetic rats treated with losartan 〔20mg/(kg*d)〕(DL) and diabetic rats treated with perindopril （2mg/(kg*d)〕(DP).Urinary albumin was observed at the 4th、8th、12th and 16th week.Results　Urinary albumin excretion of diabetic rats treated with losartan or perindopril were significantly lower than that of diabetic untreated group (P＜0.01).The effect was not different between losartan treated and perindopril treated rats.Conclusion　The results suggested that losartan can reduce urinary albumin excretion in diabetic rats.

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Effects of taurine treatment on glutamate transporter mRNA expression in diabetic rat retina

Zhaoxia XU ; Mantian MI ; Hongxia XU

Chinese Journal of Diabetes.2005;13(5):340-342,345.

Objective To investigate the effects of taurine treatment on retina ultrastructure and glutamate receptor GLAST mRNA expression in diabetic rat.Methods Diabetic rats were fed with 1.2% taurine for 3 months.Reverse transcription and polymerase chain reaction(RT-PCR) were employed to detect the expression of GLAST mRNA.Changes of retinal ultrastructure of rats were observed with transmission electronic microscope. Results From the first to the third month,the(mRNA) expression of GLAST gradually decreased in diabetic rat retina.Taurine could up-regulate the expression in this period.Membrane disc arrange was disordered and mitochondria swelled in STZ diabetic rat retina,which were ameliorated after taurine treatment.Conclusion Taurine can up-regulate the mRNA expression of GLAST and restrain excitotoxicity of glutamate.These observations suggest that taurine may play a role of nerve protection.

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Gene transfer and expression of human insulin gene in fibroblasts in vitro

Haiying XIE ; Yancheng XU ; Jiazhong SUN

Chinese Journal of Diabetes.2005;13(5):389,391-.

Human insulin gene(PCMV.INS) is successfully transferred and expressed into N1H3T3 cell by chitosan mediation.This is helpful in the research of the gene therapy of type 1 diabetes.

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Beneficial effects of nitrendipine on diabetic cardiomyopaty in rats

Cai LI

Chinese Journal of Diabetes.1993;0(00):-.

The effects of nitrendipine (Nit) on myocardial contractility, myocardial succinate de-hydrogenase (SDH) and cytochrome oxidase (CCO) activities in streptozotocin (65mg/kg) diabetic rats were studied. Four weeks after the induction of diabetes, the rats were treated with Nit (30mg ?kg -2/day) for 4 weeks. The results showed that ventricular diastolic function was affected after 4 weeks of diabetes, and both ventricular diastolic and systolic functions were obviously involved after 8 weeks. These changes were significantly improved in diabetic rats receiving Nit treatment. The myocardial SDH and CCO activities in diabetic animals were markedly lower as compared to controls. The attenuation of these enzyme activities in diabetic rats was significantly reversed by administration of Nit. These findings suggest that Nit treatment may exhibit some beneficial effects on diabetic cardiomyopathy.

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A study on the mRNA expression of the glucose transporter gene in the diabetic rats

Hua WANG

Chinese Journal of Diabetes.1993;0(00):-.

The study was proceeded on the mRNA expression of the GULT1 and GLUT4 gene in the alloxan diabetic rats by the technique of dot-blot hybridization analysis. The results showed that the levels of the GLUT4 mRNA expression were reduced in diabetic rats while increased in insulin treated diabetic rats. This suggests that the reduced GLUT4 mRNA expression which could affect the quantity of its posttranslational products may be one of the causes of the insulin resistance.

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Effects of thymosin and insulin on suppressor T cell in type Ⅱdiabetes

Kaizhen ZHANG

Chinese Journal of Diabetes.1993;0(00):-.

The activity and the percentage of suppressor T cells in PBMC from 28 patients with type I diabetes of various durations treated with thymosin or insulin were detected by using the method of ConA-induced suppressor T cells and Wu T8 (suppressor/cytotoxic) monoclonal antibody respectively. Both thymosin and insulin were found to have ability to improve and normalize the ConA-induced suppressor cell activity and the percentage of Wu T8 cells in diabetic patients. The maximal effects were found at the concentration of 3. 22?mol/L thymosin and 1. 722nmol/L(240. 05mIU /L) insulin. Thymosin was more effective than insulin.

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A Study on the Na+-K+ATPase Activity of Erythrocyte Membranes in Diabetic Patients

Guizhi LU

Chinese Journal of Diabetes.1993;0(00):-.

A study on the Na+-K+ ATPase activity of erythrocyte membranes was carried out in 34 NIDDM patients without complications and in 20 age-matched normal control subjects. The Na+-K + ATPase activity was decreased in the NIDDM patients as compared with that of the normal subjects. There was a positive correlation between the Na+-K+ ATPase and the serum insulin peak level as well as the area under curves of insulin releasing test. There was no correlation between the Na+-K+ATP ase and the fasting blood glucose or glycohemoglobin level. The mean value of erythrocyte su-peroxide dismutase (SOD) was decreased in the NIDDM patients as compared with that of normal subjects. But the Na+-K+ ATPase activity was not correlated with SOD. The result suggests that Na+-K+ATPase activity in the NIDDM patients is regulated by the serum insulin level. Insulin treatment would be useful for these patients to increase the activity of Na+-K+ATPase and prevent chronic complications.

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A study on the polymorphisms and haplotypes at the apoAI-c Ⅲ gene cluster from the patients with NIDDM

Jinchao ZHANG

Chinese Journal of Diabetes.1993;0(00):-.

The polymorphisms and haplotypes identified by restriction fragment length polymorphisms (RFLPs) at the apolipoprotein A1-C3 gene cluster (ApoAl-C3) were studied in 75 Han-nationality-Chinese patients with NIDDM, 30 of which were complicated with hypertriglyceridemia. Fifty unrelated healthy subjects matched for age and body weight were chosen as controls. The results showed that after the RFLPs hydrolyzed by the restriction endonucleases SstI and MspI were hybridized with the human ApoAl gene cDNA fragment(l. 0 kb),S2 and M2 tnutational sites consistent with other reports were found . Significant frequency difference in genotype M1M1 was observed between the simple NIDDM and control group(62% vs 42%). In the NIDDM with hypertriglyceridemia group,both of the S2S2 and M2M2 genotypes had a frequency of 10% and the haplotype S2-M2 had a higher frequency than that in controls. This suggests the association of S2M2 haplotype with NIDDM complicated by hypertriglyceridemia.

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Effect of islet amyloid polypeptide(Amylin) on glucose metabolism and secretion of insulin in the rats

Yuning QIAN

Chinese Journal of Diabetes.1993;0(00):-.

The effect of continuous amylin infusion on glucose tolerance in rats and the effect of amylin on glucose-stimulated insulin secretion from isolated rat pancreatic islets were sudied. Three groups of animals formed the study of glucose tolerance: Group 1 received salined infusion; Group2 , amylin at 2. 6 nmol?kg-1/h;and group 3,amylin at 26 nmol**kg-1/h. The total infusion time was 60 min. The insulin level and serum glucose were not altered at lower dose of amylin. However glucose levels markedly rose at 2.7,12 min. after beginning IVGTT, without significant change of insulin levels in group 3. The response of insulin to 16. 7 mol glucose-stimulated isolated rat pancretic islets was singnificantly inhibiled by the addition of amylin 10 umol.

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