Journal Detail Information

1

Cite

Share

Effect of microsomal triglyceride transfer protein(MTP) gene polymorphism on angiopathy in type 2 diabetes

Chinese Journal of Diabetes.2006;14(2):98-100.

Objective To investigate the relationship of MTP gene variation with type 2 diabetic angiopathy. Methods PCR and restriction-fragment-length polymorphism(PCR-RFLP) analysis and gene sequencing were used to investigate the influence of a common MTP gene polymorphism in promoter region on diabetic macroangiopathy. Results (1) A single nucleotide mutation of G→T was detected at 493 bp of upstream from the transcriptional start point.There was a significant difference in genotype frequency(χ2=9.162,P=0.01) and T allele frequency(χ2=10.322,P=0.001) between macroangiopathy group and non-macroangiopathy group.(2) MTP-493 TT group had the significantly higher serum levels of TC, TG, LDL-C, VLDL-C and lower HDL-C than other genotype groups.(3) Multivariate stepwise regression analysis showed that T allele, age, disease duration, diastolic blood pressure and total cholesterol were independent risk factors against type 2 diabetic macroangiopathy.The HDL-C level was negatively related with diabetic macroangiopathy. Conclusions MTP-493 TT genotype may be deeply involved in production of atherogenic dyslipidemia and associated with development of macroangiopathy in type 2 diabetic subjects.

2

Cite

Share

Insulin resistance and adrenal androgen excess in adolescents with polycystic ovary syndrome

Hong TAO ; Shuhua MI ; Zhaolin LU

Chinese Journal of Diabetes.2006;14(3):162-165.

Objective To understand the correlation between insulin resistance(IR) and adrenal androgen excess in adolescents with polycystic ovary syndrome (PCOS). Methods According to BMI, 90 adolescents with PCOS were divided to groups of normal weight PCOS(NW-PCOS,33 cases,BMI<25.0 kg/m2) and obesity PCOS(OB-PCOS,57 cases, BMI≥ 25.0 kg/m2),and 15 normal weight healthy girls matched for age as controls.All patients had normal OGTT.Acute ACTH stimulating test was performed on patients, and cortisol (F), 17-hydroxyprogesterone(17-OHP), dehydroepiandrosterone(DHEA) during ACTH testing at 0 and 60 minute (steroid0 and steroid60) were determined. Results Compared with controls, NW-and OB-PCOS patients showed significantly greater levels of total testosterone (T), Luteotropic hormone(LH), FIns, 2 hIns, and HOMA-IR(P<0.01).The freqnencies of hirsuties and acne, and serum level of FIns, 2 hIns, and HOMA-IR were significantly higher and LH levels were much lower in the group of OB-PCOS than those in NW-PCOS(P<0.05 and P<0.01).ACTH stimulating test showed that PCOS patients had significantly higher levels of basal 17-OHP0, DHEA0, as well as 60-minute ACTH-stimulated levels (Steroid60) of 17-OHP60、DHEA60, and net increase of 17-OHP(Δ17-OHP)as compared with normal controls(P<0.05 and P<0.01).The 60-minute ACTH-stimulated levels of 17-OHP60, DHEA60,Δ17-OHP, and ΔDHEA were also higher in group of OB-PCOS than those in group of NW-PCOS(P<0.05 and P<0.01), and there were no obvious discrepancies in basal 17-OHP0, DHEA0 between OB-PCOS and NW-PCOS groups.Spearman correlation analysis showed that in group of OB-PCOS, HOMA-IR value was significantly associated with BMI, 17-OHP60, Δ17-OHP, DHEA60, ΔDHEA(P<0.05 and P<0.01); whereas in group of NW-PCOS, HOMA-IR was associated only with BMI(P<0.01). Conclusions The significantly higher basal and ACTH-stimulated levels of 17-OHP and DHEA indicates adrenal hyperresponsiveness in adolescents with PCOS.Adrenal androgen excess appears to be closely associated with insulin resistance in obese adolescents with PCOS

3

Cite

Share

Study on the association between the different levels of C-peptide of type 2 diabetes and colorectal cancer risk

Yikun ZHOU ; Qiang GUO ; Yuanming XUE

Chinese Journal of Diabetes.2008;16(4):231-232.

To study on the association between the different levels of C-peptide of type 2 diabetes and colorectal adenoma and cancer risk.We detected the concentration of serum C-peptide in 45 type 2 diabetes who were diagnosed as colorectal adenoma and cancer.It showed that serum C-peptide level was higher in type 2 diabetes with colorectal adenomas and cancer than without colorectal adenomas and cancer.The serum C-peptide level may have relationship with colorectal adenomas and cancer.

4

Cite

Share

The association of type 2 diabetic retinopathy with FFA and oxLDL-C

Ruihua LI ; Guangyao SONG ; Junjiang LIU

Chinese Journal of Diabetes.2008;16(4):228-230.

Objective To study the relationship of free fatty acids and oxLDL-C with diabetic retinopathy(DR).Methods The 108 type 2 diabetes cases with(n=68)and without(n=40)DR were enrolled,and compared with 42 health control.FFA was determined by copper chromogenic method.oxLDL-C was determined by ELISA with kits provided.The correlation of risk factors with retinopathy was analyzed by logistic regression analysis.Results FFA and ox LDL-C levels were higher in DR group than in groups of DM and control.Logistic regression analysis showed that as the independent risk factors for DR pathogenesis,LDL-C,FFA,oxLDL-C and course of disease entered into regression equation.Conclusions FFA and oxLDL-C are the important risk factors for DR.

5

Cite

Share

The relationship between the serum ghrelin level and β-cell function after treatment of patients with newly diagnosed type 2 diabetes mellitus

Ying HU ; Qiao ZHANG ; Lixin SHI

Chinese Journal of Diabetes.2008;16(4):218-220.

Objective To investigate the relationship between the serum ghrelin level and β-cell function after treatment of patients with newly diagnosed type 2 diabetes mellitus.Methods 34 patients with newly diagnosed type 2 diabetes mellitus were divided into three groups of pre-treatment(DM0),two weeks after treatment(DM1)and six months after treatment(DM2).Intravenous glucose tolerance tests(IVGTT)were performed and blood glucose,HbA1C,ghrelin,insulin were measured during the study.Insulin sensitivity was assessed by homeostasis model assessment(HOMA).Results(1)Compared with DM0 group,beta-cell function and the mean AUC of insulin were dramatically improved in groups of DM1 and DM2(all P<0.05).(2)Compared with DM1 and DM2,ghrelin levels and the mean AUC of ghrelin in DM0 groups were lower(all P<0.05).(3)Only positive correlation between ghrelin and HOMA-β(r=0.446,P<0.05),and negative correlations of ghrelin with glucose(r=-0.274,P<0.05),insulin(r=-0.35,P<0.05)and BMI(r=-0.276,P<0.05)were found after six months of treatment.Conclusions Ghrelin might play an important role in the pathogenesis and progress of type 2 diabetes mellitus.

6

Cite

Share

Psychological disorders and their effect on the therapeutic efficacy in type 2 diabetes

Kunning SHAO

Chinese Journal of Diabetes.2008;16(5):301-303.

Objective To investigate the psychological disorders and their effect on the therapeutic efficacy in type 2 diabetes(T2DM).Methods FBG,PBG,HbA1c,TG,CH,urine albumine,urine Cr were tested in 120 T2DM patients(male 51,female 69),simultaneously the psychological status was evaluated with the measuring scales of SDS,SAS,PEI and EPQ.The effect of abnormal psychological status on treatment of type 2 diabetes was analyzed.Results 32.5% patients had depression,and 18.3% had anxiety.The scores of the patients were remarkably higher than those of the controls.The total scores of E,N,P of EPQ were higher and the score of PEI was lower in the patients with T2DM than those in the healthy subjects.Diabetes disease status of the patients with psychological disorders was more severe than that of the patients without abnormal psychology.Conclusions Psychological disorders are common in T2DM patients.The psychological disorders can effect the treatment effect and prognosis of type 2 diabetes.

7

Cite

Share

Study on the changes of the protein conformation of gestational diabetes mellitus (GDM) placenta tissue by using Raman spectroanalysis

Ying CHANG ; Xiumin NIU

Chinese Journal of Diabetes.2008;16(5):294-296.

Objective To study the protein conformational change of placenta tissue by using Raman spectrum.Methods By using Raman spectroanalysis,we detected the placenta protein conformational change of GDM and control groups.Results(1)In the placenta of GDM,the absorption bands of tryptophan and phenylalanine were increased obviously.(2)In the placenta of GDM,the secondary structure of protein was composed of α-helix,random coil and β-sheet.Conclusions In the placenta of GDM,the orderly conformations of main chains in protein are decreased.Side chains of amino acids,especially tryptophan and phenyl-alanine,are changed greatly.The structure variation of protein may be correlated to the diabetic complications.

8

Cite

Share

Study on the mtDNA tRNALeu(UUR) mutation in diabetics with onset age less than 45 years

Demin YU ; Mingzhen LI ; Demin LIU

Chinese Journal of Diabetes.2005;13(2):98-101.

Objective To investigate the prevalence of mtDNA A3243G mutation in the early onset diabetics in Tianjin for exploring the relationship between mtDNA mutation and diabetes. Methods 348 kinship-free diabetics whose ages at onset were less than 45y were randomly recruited, with 207 control subjects. The PCR-RFLP and cloning techniques were applied to screen the A to G substitution at nucleotide 3243 of mtDNA tRNALEU（UUR）. Meanwhile, the clinical and genetic analysis was done from one pedigree. Results We detected two diabetics harboring the well-known a3243g mutation with the mutation frequency of 0.6%. While the mutation frequency in diabetics with （positive） family history was 1.2%, and zero in the control subject. The proband showed a typical （picture） of mitochondrial diabetes mellitus, while the other family members harboring the same （mutation） had heterogeneous presentation. Conclusion The prevalence of mtDNA A3243G mutation in early onset diabetes of Tianjin is low, but relatively higher in diabetes complicated with other （symptoms） of mitochondrial disease. Furthermore, its heterogeneity would decrease with aging in （mitotic） tissue

9

Cite

Share

The clinical characteristics of the familial early onset type 2 diabetes mellitus

Xueyao HAN ; Linong JI

Chinese Journal of Diabetes.2005;13(2):83-86.

Objective To investigate the clinical characteristics of the familial early onset type 2 diabetes（T2DM）,and the factors affecting the age at diagnosis. Methods 190 probands from early onset T2DM pedigrees （diagnosed before 40 years old） and 103 probands from late onset T2DM （pedigrees）（all patients were diagnosed after 40 years old）were included in this study. The homeostasis model assessment of insulin resistance （HOMA-IR）was used to estimate insulin resistance, HOMA-β cell was used to evaluate basal insulin secretion. The ratio of the incremental insulin (ΔI30) over （incremental） glucose(ΔG30) was calculated after intake of a steamed bread made of 100g flour to （evaluate） the early insulin secretion. Body mass index（BMI）and serum lipid levels were also （determined.）（Results）（1）The early onset group had significantly higher fasting serum insulin （（natural） logarithm transformed, 2.6±0.6 vs 2.4±0.5 μU/ml, P<0.05）, triglycerid （TG, 1.7±2.0 vs 1.4±2.0 mmol/L,P<0.05）,HOMA-IR（natural logarithm transformed, 1.6±0.8 vs 1.3±0.6, P<0.01）and lower high density lipoprotein （HDL-C, 1.2±0.3 vs 1.3±0.3mmol/L,P<0.05） than those in late onset group.（2）Linear regression analyses showed that HOMA-IR, HOMA-β were （independent） risk factors predicting early age of onset. （3）In the early onset type 2 diabetic patients, the subgroup with BMI more than 25 kg/m2 had significant earlier diagnosis age （32±7 vs 34±5 y, P<0.05）, lower level of serum HDL-C （1.1±0.3 vs 1.3±0.3 mmol/L, P<0.01）, higher FIns （（natural） logarithm transformed, 2.8±0.6 vs 2.4±0.6, P<0.01）, higher HOMA-IR （natural （logarithm） transformed, 4.3±0.9 vs 3.9±1.0, P<0.01）and higher TG （1.9±2.0 vs 1.5±2.1 mmol/L, P<0.01）than those with BMI less than 25 kg/m2. Conclusion Compared with late onset familial type 2 diabetic patients,more severe insulin resistance and relative β cell function defect are the important clinical （features） in early onset familial type 2 diabetic patients. The interaction between （insulin resistance） and （β-cell） dysfunction are probably the determinants of onset age of T2DM.

10

Cite

Share

The effects of blocking renin-angiotensin system with losartan or perindopril on renal angiotensin Ⅱ and its type 1 receptor expression in STZ-induced diabetic rats

Dongmei ZHANG ; Huiju ZHONG ; Min ZHOU

Chinese Journal of Diabetes.2005;13(2):131-133.

Objective To observe the effects of blocking renin-angiotensin system with losartan or perindopril on renal angiotensinⅡand its type 1 receptor （AT1R） expression in STZ-induced （diabetic） rats. Methods Male SD rats were randomly divided into 2 groups: normal control group （NC） and （diabetic） group. Diabetes was induced by STZ （65 mg/kg） abdominal injection. Four weeks later, diabetic rats were further divided into 4 groups: diabetic rats treated with losartan (DL, 20 mg·kg-1·d-1),diabetic rats treated with perindopril (DP,2 mg·kg-1·d-1) and diabetic untreated control group（DC）. Radioimmunoassay was used to measure plasma and renal angiotensin （Ⅱ.）Expression of AT1R was detected by RT-PCR in each group before and after 12 week′s treatment. Results At the 4th week, renal AT1R expression decreased. At the 16th week, plasma angiotensinⅡ,renal angiotensinⅡand AT1R mRNA in diabetic untreated group were significantly higher than those in normal rats. （Renal） angiotensinⅡand AT1R in losartan or perindopril-treatmented group were significantly lower than those in DC group. Conclusion Intrarenal RAS is abnormal in STZ-induced diabetic rats. Renoprotective effects of losartan and perindopril may partly induced by inhibiting renal angiotensinⅡ and AT1R （expression.）

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share