The association of glucokinase(GCK) gene with type 2 diabetes in Chinese was studied by using the polymerase-chain-reaction amplified microsatellite polymorphism marker, GCK-5'. In comparison with non-diabetics,allele 4 was significantly increased in type 2 diabetics(X2 = 6. 773,P=0. 009). The frequency of genotype 44 and 4X was significantly higher in type 2 diabetics than in non-di-abetics(X2 = 6. 439, P = 0.011). The allelic frequency difference was also observed in overweight/obese subgroup compariscn(X2 = 7. 718,P = 0. 021). The risk of type 2 diabetes in Chinese with genotype 44 or 4X is about 3. 6 times higher than in Chinese with genotype XX. Our results indicate that GCK is associated with type 2 diabetes in Chinese.