Journal Detail Information

1

Cite

Share

Morbility rate and composition analysis of malocclusion of resident in bronze age from Yanghai cemetery, Xinjiang

Zheng LIU ; Yang GAO ; Quanchao ZHANG ; Hong ZHU

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To investigate and analysis the situation of malocclusion of 49 skulls of ancient resident in Xinjiang, and discuss the history evolution and the pathologic mechanism of malocclusion. Methods The investigation was carried out based on the standard of individual normal occlusion. Results Among the group of 49 skulls, the morbility rate of malocclusion was 30. 61% ; in Angle's classification of malocclution, class I was the most (73. 33%); in Mao's classification of malocclution, class I was also the most (33. 33%). Conclusion The morbility rate of malocclusion of resident in bronze age is lower than that of modern resident and nearer ages resident. There is a close releationship between the human food construction and the pathogenesis of malocclution.

2

Cite

Share

Epidemiology study of Eales disease and its risk factors in healthy young men

Jun XIAO ; Shihui WEI ; Jie WANG ; Yao HE

Journal of Jilin University(Medicine Edition).2006;0(01):-.

0. 05), and there seemed to have some statistical correlation between the incidence of Eales disease and PPD (P

3

Cite

Share

Prokaryotic expression of human L-selectin and preparation of polyclonal antibody

Yanguang GAO ; Junwei FEI ; Rui FEI ; Xianlu ZENG

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To construct the expression vector and express the recombinant human L-selectin in prokaryotic system, and purify the aimed protein for the preparation of rabbit anti-human L-selectin polyclonal antibody. Methods The partial cDNA of human L-selectin was amplified from the open reading frame (ORF) of N-terminus sequence of L-selectin containing 153 amino acids by PCR, then cloned into the prokaryotic expression vector pQE40 at restriction sites BamH Ⅰ and Hind Ⅲ. The recombinant expression plasmid pQE40-L-selectin was transformed into E. coli M15 for expression. The fusion protein including 6-His-tag was purified by Ni-NTA chromatographic column and analysed by SDS-PAGE. The purified protein was used to immune rabbit for preparing polyclonal antibody. Western blotting analysis and dot immunoblot assay (DIBA) were used to test the titer of the antiserum. Results The expression plasmid pQE40-L-selectin was constructed and confirmed with restriction enzyme digestion. The quantity of the purified protein from E. coli M15 was 600 mg ? L-1. By immuning the rabbit, the polyclonal antibody was successfully prepared. The results of dot immunoblot assay (DIBA) showed that the antiserum had the high titer (1 : 1 000). Conclusion The recombinant human L-seleclin prolein can express with high efficiency in E. coli M15. The prepared polyclonal antibody has a high titer.

4

Cite

Share

Determination of Mycoplasma genitulium infection in patients with chronic non-bacterial prostatitis and its significance

Shuxia ZHONG ; Shanshan LI ; Chaoying ZHANG ; Cuiyang ZHAO ; Lei MA

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To study the action of Mycoplasma genitulium (Mg) in pathogenesis of chronic non-bacterial prostatitis. Methods Mg culture and polymerase chain reaction (PCR) were perfomed on prostatic secretion specimens from 987 patients with chronic non-bacterial pristatitis and 125 healthy men. Results ①The positive rate of Mg in the prostatic secretion from the 987 patients was 31.5% (302/987). Among the 302 positive cases, 153 cases had other pathogens, of which Ureaplasma urealyticum (Uu) was the most (128). ②In the controls, the positive rate of Mg was 2. 53% (3/125). The difference between the two groups was statistically significant (X2=44. 32,P

5

Cite

Share

Application of shaping titanium mesh by multi-point forming technique in skull repairing

Shuanglin FU ; Xuan CHEN ; Haifeng WANG ; Wenzhi FU

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To select materials with high quality and safety and evaluate the clinical value of multi-point forming technique in skull repairing. Methods 161 patients suffered from skull defect had been cured in our hospital, within them 43 patients were treated with multi-point forming technique in titanium mesh shaping, 19 patients with traditional handwork shaping, 99 patients with bone-like concrete (acrylate). The following aspects were analyzed and compared: neurosurgeons' shaping workload before operations, operative time, approving scale on shaping and complications after operation. Results Repairing skull by titanium mesh with the technique of multipoint shaping significantly shortened the average operative time to (30 ?6) min, compared with acrylate group (70?18 min) and titanium mesh traditional handwork shaping group (50?11 min) (P

6

Cite

Share

Correlations of matrix metalloproteinase content and expression with invasion and metastasis of hepatocellular carcinoma

Zhichao ZHANG ; Yaxin SUN ; Mingku JIA

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To investigate the correlations of serum matrix metalloproteinase-2, -9 (MMP-2, MMP-9) contents and tissue expressions in hepatocellular carcinoma with tumor invasion and metastasis. Methods Serum MMP-2, MMP-9 contents were detected in 40 patient with hepatocellular carcinoma and 20 healthy controls by ELISA; the expressions and distributions of MMP-2 and MMP-9 in 40 patients and 10 normal tissues were detected by immunohistochemical method. Results Serum MMP-2, MMP-9 contents were significantly elevated in cancer samples compared with normal serum (P

7

Cite

Share

Significance of pathological changes and prognosis in chronic hepatic disease by assay of plasma level of neuropeptide Y

Ligang CHEN ; Baorong CHI

Journal of Jilin University(Medicine Edition).2006;0(01):-.

0. 05); but plasma levels of NPY in patients with hepatic cirrhosis and ascites or those with HRS had significant difference (P

8

Cite

Share

Relationship between oncogene Stat3 and invasion and metastasis of non-small cell lung cancer

Yaxin SUN ; Zhichao ZHANG ; Chuanjie XU ; Chunlei YU

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To investigate the expressions of Stat3, CyclinD1 and proliferating cell nuclear antigen (PCNA) in non-small cell lung cancer (NSCLC), and analyze their relationships with carcinogenesis and progression of NSCLC. Methods SP immunohistochemistry was used to detect the expressions of Stat3, CyclinD1 and PCNA in 45 cases of NSCLC. Results The positive rate of Stat3 was 71. 1 % in NSCLC. The expression intensities of Stat3 was not associated with different tumor sizes and histological type, but it was associated with the expression intensities of CyclinDi and PCNA (r= 0.531, P

9

Cite

Share

Expressions of matrix metalloproteinases-2 and its tissue inhibitor-2 and microvessel density in endometrial carcinoma

Yujing ZHANG ; Liping GU ; Ying HUA ; Shoujie ZHANG

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To study the expressions of matrix metalloproteinases-2 (MMP-2) and its tissue inhibitor-2 (TIMP-2) and microvessel density (MVD) in human endometrial carcinoma tissues and their relations with the invasion and metastasis of endometrial carcinoma. Methods Immunohistochemistry was used to measure the MMP-2, TIMP-2, and MVD protein levels in 33 patients with endometrial carcinoma and 8 normal endometrial samples. Results The MMP-2, TIMP-2 and MVD protein expressed in both normal endometrial samples and endometrial carcinoma. The positive expression proportions of MMP-2, TIMP-2, and MVD protein in endometrial carcinoma tissues were higher than those in normal endometrial samples (P

10

Cite

Share

Effects of rhBMP_2/ TGF-? on heterotopic osteogenesis of autogenous marrow stromal cells and ?-tricalcium phosphate scaffold

Wei SUN ; Zhanxin WANG ; Jinsong GAO

Journal of Jilin University(Medicine Edition).2006;0(01):-.

Objective To observe heterotopic osteogenesis of autogenous marrow stromal cells on ?-tricalcium phosphate (?-TCP) scaffold, and the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) and transforming growth factor-beta (TGF-?) on osteogenesis. Methods The Newzealand rabbits autogenous marrow stromal cells were cultured by mineralized condition medium. The cell suspension was collected. All the cells with concentration of 5 ?106 ? mL-1 were divided into four groups. Group A: rhBMP-2 (15 ?g) /TGF-? (30 ng) were added; group B: TGF-? 30 ng was administered; group C: without growth factor as control; group D: continual cultured and no loading on ?-TCP. MSCs in group A, B, C were planted on ?-TCP. Alkaline phosphatase (ALP) activity was examined by histochemistry. Then MSCs loading on ?-TCP were embedded in autogenous subcutaneous. Four weeks later, the osteogenesis was observed by HE staining, type I collagen and bone morphogenetic protein synthesis were examined by immunohistochemistry stains. Results The ALP activities in groups A and B were significantly higher than those in group C and D (P

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share