Objective To confirm the difference in the biological characteristics between V?24 natural killer T cells(NKT) and the cytokine-induced killer cell(CIK).Methods V?24 NKT cells and CIK cells were expanded from human peripheral blood mononuclear cells.Purified TCRV?24~+ NKT cells and CD3~+CD56~+CIK cells were obtained by using Dynal beads.The phenotype and the levels of cytokine and necrosis-related factors expression on the purified NKT cells and CIK cells were determined by flow cytometry.The cytotoxicity of the purified NKT cells and CIK cells were measured by means of DIOC18 staining and flow cytometry.Results The proportions of TCRV?24~+V?11~+NKT cells and CD3~+CD56~+CIK cells were elevated up to 90% after purification.The majority of V?24 NKT cells were CD4~+ and DN NKT cells.They demonstrated high levels of expression of TCRV?24,V?11,CD3 and CD161 and a low level of expression of CD56,but most purified CD3~+CD56~+CIK cells were CD8~+ T cells,with high levels of CD3 and CD56 expression,low level CD161 expression and little TCRV?24 and V?11expression.After antigen stimulation,the purified CD3~+CD56~+CIK cells showed higher levels of expression of IFN-?,TNF-?,Perforin,FasL and TRAIL,compared to the NKT cells.CD3~+CD56~+CIK cells secreted little IL-4,whereas,V?24~+NKT cells secreted high level of IL-4.In addition,the cytotoxic effect of the CD3~+CD56~+CIK cells on tumor cell lines K562,U937 and Jurkat were more intense than that of the NKT cells.Conclusion It is evident that TCRV?24~+ NKT cells and CD3~+CD56~+CIK cells differ absolutely in many ways and might play different roles in anti-tumor immunity and immune regulation.

One hundred unrelated healthy indi- viduals of the Mulao nationality were ty- ped for HLA-A, -B, -C, -DR and -DQ locus antigens. The distribution of HLA class I antigens is similar to that of Zhu- ang, Miao and Yao nationalities in the Guangxi Area and to that of the Han po- pulations in southern China. For example, A2, A9 and A11 are much more frequent in A locus, as well as B40, B13 and Bw46 in B locus and Cw3. Cw1 (including Cx46) and Cw7 in C locus. The antigen B17 sho- wing a lower frequency in Mulao natio- nality than in other populations in sou- thern China is quite especial. The distri- bution of DR and DQ antigens in HLA class II shows still more different between Mulao and Zhuang nationalities. It is not clear whether the origin of the two na- tionalities or other factors give rise to their difference, and it needs further study.

A study of the mutagenieity of SAGS preservative solution for red cells,performed by Ames method, was reported.Result of the study in- dicated that there was no evidence of the positive mutagenicity reaction of SAGS preservative solution for red cells at its 6 doses ranging from 0.02 to 100mg/ml,whether the rat liver microsomal enzymes(S9)were added as metabolic activators or not.That is, the solution was unable to inducethe mutation at gene loci in the strains tested by Ames Method.

The absorption of anti-A and anti-B in reagent sera containing non-ABO antibodies,performed by coupling the ABH substance to se- pharose 4B,was introduced.The sera containing both ABO antibodies and anti-D(11),-C(5),-E(5),-c(6), -e(4),-M(1),-N(1),-s(1),-K(1), -Kp~b(1),-Fy~a(1),-Js~b(1)absorbed by this method,were tested then by the ABO-incompatible cells lacking relevant non-ABO antigens,and the panel red cells(O).As a result,all the ABO antibodies were absorbed exclusively,but all the non-ABO antibodies were preserved fullyThe non-ABO antibodies can't give the false positive reaction and their titer can't decrease after repeated absorp- tion.The advantages of this method and the factors that have influeced on effectiveness of the absorption were discussed.

A case of B, -D- blood groups was found in a Chinese woman whose baby died of haemolytic disease of newborn, Her husband was AB, CCDee blood groups. The unexpected antibodies in her sera were studied. The sera reacted with the red cells of all common Rh blood groups except -D-, (Rh)~(mod) and (Rh)~(null). Both respective and serial absorption and elu- tion tests were done for the ccDEE, CCD ee and ccde red cells. The results show that there are two antibodies in her sera. The anti-e is separable and the anti -Hr_o can react with all of the common Rh blood groups. The results were the same to that reported by Allen and Corocoran in 1958.

Effect of both granulocyte transfusions and antibiotics on the treatment of acute agranulocytosis with severe infections in 9 of 15 patients were reported. However, all 6 patients with agranulocytosis ind- uced by various drugs survived from sev- ere infections. It revealed that granulocyte count of each transfusion was more sign- ificant for the therapeutic effect.

An epidemic of hepatitis C virus occurred in plasmapheresis donors on Guan county in 1985. Early on 1986, there were investigated the state of hepatitis C virus and hepatitis B virus infection in plasmapheresis and whole blood donors from the villages with plasmapheresis donation in that year 1985, the villages with five-year plasmapheresis donation and the villages bacially without blood donation, then the follow-up survey of them was made in 1990. The positive rates of anti-HCV for plasmapheresis donors in 1986 and 1990 were 64.7% and 43.9% respectively. The positive rates of anti-HCV in the plasmapheresis donors with abnormal ALT were 77.5% and 81.1% and in the plasmapheresis donors with normal ALT were 51.3% and 34.3%. The positive rate for anti-HCV in plasmapheresis donors was higher than that in the whole blood donors and controls. It was proposed that the increasing of anti-HCV screening in blood supplying villages was imperative. In addition, the follow-up survey of 91 anti-HCV positive plasmapheresis donors was made in the fifth year since then, the rate at which the positive results turned to negative was 30.8%, about 70% cases turned out to chronic.

The effect of an specific immunoglo- bulin to meningococcus group A was tes- ted in rabbits infected with meningococcus group A and yielded satifactory results All the 10 rabbits in the treated group appeared normal, while 5 of the 12 rabbits in the control group developed toxic symptom and DIC, and 2 died finnally. The immunoglobulin was then appli- ed clinically to 4 children with meningo- coccal meningitis. Three of them recove- red. Unfavorable outcome was found in the fourth patient, in whom meningococ- cus group B was isolated from his blood and CSF. Since meningococcus group A continues to be the predominant pathogen in our country, the above-mentioned study provides strong support for the adminis- tration of specific immunoglobulin to chil- dren with meningococcal meningitis.

ABO blood group of 236 patients with aplastic anemia (AA) was compared with that of 2277 healthy individuals. Subjects with A antigen were susceptible to AA (R=1.3090, x~2=3.8489, P

A total of 272 lots of immunoglobulin products was investigated, and the anti- body potency of measles was determined by hemogglutination inhibition test. The total antibody potencies of diphtheria, pertusis, rubella, epidemic hemorrhagic lever and titer of anti-HBs and anti-HAY were tested in some lots of immunoglobulin produced in recent years. The results provided a scien- tific basis for clinical use.