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Study on the effect of microparticle platelet frozen-powder on the healing of skin wound in mouse

Jing LI ; Ruqing LI ; Xin WANG ; Shuming ZHAO

Chinese Journal of Blood Transfusion.2008;0(11):-.

0.05). Levels of TGF-?1 and PDGF in microparticle platelet frozen powder were higher than those in fresh platelet(P

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Investigation of the polymorphism of HPA-1-17 gene and the platelet transfusion refractoriness in Li population on Hainan island,in China

Lan XIA ; Xingquan CAI ; Xinping CHEN

Chinese Journal of Blood Transfusion.2008;0(11):-.

Objective To investigate the allele polymorphism and characteristic of HPA-1-17 genotyping of Li ethnic on Hainan island,and to evaluate the HPA mismatched ratio between donor and recipient in randomly chosen cases of blood transfusion,in order to investigate the platelet transfusion refractoriness in Li population.Methods Genotyping tests of A-1—17 allele of the blood sample from 180 Lis were conducted with PCR-SSP.Results A total of 180 blood samples were genotyped successfully in HPA genetic system,among which 0.9972 were HPA-2a,0.0028 were HPA-2b,0.4889 were HPA-3a,0.5111 were HPA-3b,0.9667 were HPA-5a,0.0333were HPA-5b,0.9972 were HPA-6a,00028 were HPA-6b,0.4250 were HPA-15a,0.05750 were HPA-15b,and they all showed polymorphism. But polymorphism was not detected out in other allelic genes,such as HPA-1,-4,-7—14,-16,and -17. The bb homozygous were detected in two alleles,among which 0.2834 was HPA-3 and 0.3667 was HPA-15,but it wasn't detected in the others systems. In the randomly chosen cases of blood transfusion,the mismatch rate of HPA-3 ,HPA-15 and HPA-5 was 37.49%,36.93% and 6.23%,respectively.Conclusion This research revealed the distribution of HPA gene in Li population on Hainan island,and found the allelic frequency and thier characteristics. According to our findings,HPA-2,HPA-3,HPA-5 and HPA-15 genes between donor and recipient should be matched in order to avoid platelet transfusion refractoriness.

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Dongfeng ZENG ; Peiyan KONG ; Xinghua CHEN

Chinese Journal of Blood Transfusion.2008;0(11):-.

Objective To clarify the risk,treatment and preventive measurement of transfusion related acute lung injury (TRALI) occurred in HLA half-matched hematopoietic stem cell transplantation. Methods A case of TRALI occurred in HLA half-matched hematopoietic stem cell transplantation was analyzed,including the clinical characteristics,the laboratory and instrumental examination,the treatment measure and prognosis,and then the associated literature was reviewed.Results Owing to the blood products(fresh platelet) transfusion during transplantation,the patient got TRALI. And after active rescue,the patient eventually died due to the worsen condition.Conclusion The risk of TRALI is very high in HLA half-matched hematopoietic stem cell transplantation since the blood products transfusion is inevitable,so the effective and timely treatment and preventive measurement are necessary.

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Reducing the risk of ABO incompatibility by two specimen policy

Peiyuan ZHU ; Fengxia DONG ; Jianfeng LUAN

Chinese Journal of Blood Transfusion.2008;0(12):-.

Objective:To evaluate the effectiveness of using two specimens for ABO grouping on the prevention of ABO-incompatible transfusions caused by sample errors. Methods A two specimen policy for blood samples of pre-transfusion testing was implemented from July 2007. This policy was defined as that for patients requiring transfusion or likely to require transfusion,ABO group was determined twice on 2 separate-samples. The rate of mislabeled samples and miscollected samples prior to blood transfusion were recorded and analyzed.Results From Jul 2007 to Jun 2009,79 115 samples for pre-transfusion compatibility testing were accepted. A total of 273 samples were identified as mislabeled. The rate of mislabeled samples was 1 in 290. The rate of miscollected samples with correction for silent errors was 1 in 1485 (23 miscollected samples of 47 507 repeat samples,with a correction factor of 1.391 2). Of the 23 miscollected samples detected by double-step typing policy,9 cases (39%) could have caused ABO-incompatible transfusions.Conclusion The risk of ABO-incompatible transfusions caused by mislabeled and miscollected samples can be reduced by two specimen policy.

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Red Cells Suspended in SAGS Medium

Shu-Ping YANG ; Jing QI ;

Chinese Journal of Blood Transfusion.1988;0(01):-.

Red cell concentrates (Hct≥90%) weresuspended in SAGS medium(RCS.contain-ing sodium chloride,adenine,glucose,sucrose),and in H(?)gman's SAGM me-dium (containing sodium chloride,adenine,glucose,mannitol).Both were stored at4?2℃ for 35 days and compared withbiochemical tests.The results of posttrans-fusion survival,ATP,2,3-DPG,pH,K~+ tests between the two preparationswere similar,and the mean values ofinvitro hemolysis of SAGS-RCS was signi-ficantly lower than that of SAGM-RCS,i。e.1.71?0.95g/L and 3.10?0.19g/L res-pectively (P

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Study of Short Chain Fatty Acids in Platelet Concentrate with Gas Chromatography

Naran BAO ; Xinmin MAO ;

Chinese Journal of Blood Transfusion.1988;0(03):-.

The short chain fatty acids inplatelet concentrate (PC) and serumwere determined by means of gaschromatography.The short chainfatty acids determined includeacetic,propionic,butyric,isobuty-ric,valeric,isovaleric,lactic,pyruvic and succinic acids.Therewere acetic,lactic,pyruvic andsuceinic acids in PC;their relativecontents were 1.14,13.50,1.41 and9.54,respectively.The serum cont-ained acetic,lactic and pyruvic aci-ds;their relative contents were0.98,4.33 and 1.88,respectively.Succinic acid wasn't found in theserum,suggesting that the succinicacid in PC is the content of platelets.There was a positive correlationbetween the platelet count and lacticacid content of PC.Fresh PC wascompared with the PC of same volumepreserved for 0,24,48,72 hours atroom temperature.The lactic acidcontent of PC increased with thetime of preservation.

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A Transfusion-Associated GVHD

Baijun SHEN ; Xiaoyan LIU ;

Chinese Journal of Blood Transfusion.1988;0(04):-.

A case of the 12-year-old female patient with first relapsed acute lymphoblastic leukemia was reported. During the induction chemotherapy, the patient, transfused with whole blood and granulocytes, developed diarrhea and generalized macular rash. The skin biopsy showed some changes compatible with graft versushost disease (GVHD). Therefore, the related literature was reviewed, and the clinical features of transfusionassociated GVHD and the measures for its prevention and treatment were succinctly introduced.

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DEVELOPMENT OF INCOMPLETE ANTIBODY RAPID AGGLUTINANT AND ITS PRELIMINARY APPLICATION

Jinshen ZHAO ; Jieping KUANG ;

Chinese Journal of Blood Transfusion.1988;0(01):-.

To do rapid testing for incomplete antibody in the process of blood matching, a more suitable incomplete antibody rapid agglutinant(IRAT) and a neutralizer used in combination with IRAT were developed. The former consisted of protamine, dextran, albumin and EDTA, while the latter consisted of sodium citrate, albumin and NaCl. The method of using the agglutinant and neutralizer in testing for incomplete antibody was briefly called as IRAT method. It took 3 minutes for the method to give test result. IRAT method could be used in blood matching blood grouping aud in testing the IgG for anti-A and anti-B. Preliminary comparative observations of testing for incomplete antibody in patients with AIHA by IRTA and immediate Coombs test gave basically consistent results (Original article on page 9)

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Screening of phenothiazine photosensitizers that can be used to inactivate viruses in red blood cells

Fei WANG ; Jingxing WANG ; Xianfu YANG ; Yi HUANG ;

Chinese Journal of Blood Transfusion.1988;0(01):-.

9.9Log10 bacteriophage R 17 Conclusion Alkylation of the phenothiazine ring increased the potential of inactivating virus and decreased the damage to red blood cells? M007 might be an alternative phenothiazine dye that can be used to inactivate viruses in red blood cells?

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Investigation on SARS in Beijing volunteer blood donors

Guojing GAO ; Yan QIU ; Ping ZHANG ; Wei ZHA ; Hongying XIA ; Xiaoyan GONG ; Weijun CHEN ; Jiaming ZHU ; Hua SHAN ; Shigan LING ; Haiyan ZHAO ;

Chinese Journal of Blood Transfusion.1988;0(04):-.

Objective To investigate the epidemic of severe acute respiratory symdrome(SARS) in Beijing blood donors and make guidance for assuring blood safety during SARS epidemic.Methods Using SARSCoV Ab ELISA Kits, specimens from 2357 donors from Beijing during SARS epidemic phase,1079 preserved samples from Beijing donors collected well before the SARS epidemic,1183 donors from Shandong and Hunan provinces where no SARS had been reported were screed for IgG,IgM,and total antibodies against SARS coronavirus.Donors with reactive samples were followed up,RT PCR were performed to detect the SARS CoV RNA.Results There was no significant difference between the 3 groups of specimens and there was no SARS epidemic or subclinical SARS infections among Beijing blood donors.Conclusion Instead of blood SARS CoV Ab screening, we should focus on the donors inquiry,physical examination and education to prevent SARS transmission by transfusion.

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