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Hypotensive Test of Rats by Intravenous Immunoglobulin

Chinese Journal of Blood Transfusion.1988;0(04):-.

In the paper, 32 lots of intravenous immunoglobulin (IVIG)were tested by an animal model,the hypotensive test of rats. A linear correlation was found between prekallikrein activator (PKA)activity and hypotensive reaction if the inflow of human IVIG was made rapid (r=0.96,n=23). The preliminary observations indicated that it would be satisfactory that the PKA activity was below 35IU/ ml and the size of reduction in blood pressure was not 15% higher than that of control animal's level.The rat model can provide a method for safe usage of the intravenous immunoglobulin and reliable detection of and control over the residual PKA activity in blood products.

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Epidemiological study on HPV B19 and viral load among volunteer blood donors in Guangzhou

Yourong ZHENG ; Zhongping LI ; Haojian LIANG

Chinese Journal of Blood Transfusion.2008;0(07):-.

Objective To investigate the situation and distribution of human parvovirus B19(HPV B19) infection in volunteer blood donors in Guangzhou.Methods Blood samples from volunteer blood donors were tested for HPV B19 IgG and IgM antibody by ELISA.Real-time PCR assay was applied to detect HPV B19 DNA of HPV B19 IgG and /or IgM antibody positive specimens.Results The prevalence of HPV B19 IgG and IgM antibodies were 38.6%(679/1760) and 1.9%(33/1760) respectively,among volunteer blood donors,and there was significant difference between them((P

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Peripheral blood stem cell collection for ABO incompatible allogeneic donors and the effectiveness of transplantation

Shulai YE ; Meiai YING ; Jiaping CHEN

Chinese Journal of Blood Transfusion.2008;0(07):-.

0.05).The volumes of PBSC collected each time in the 3 groups were about 60 ml,and there were(3.67—10.25)?1010 erythrocytes per pack in the ABO major incompatible group,and 22—38 ml of plasma in ABO minor incompatible component.All the PBSC were infused to recipients without the removal of erythrocytes or plasma,and no haemolytic reaction was observed in any recipients and all their hematopoietic functions were re-established.Conclusion Enough PBSC can be harvested by using CS-3000 Plus blood cell separator from ABO incompatible allogeneic donors.By modification of separator parameters,the number of erythrocytes in the collects can be reduced.The collected PBSC can be safely infused into the blood group incompatible recipients without removal of erythrocytes or plasma.

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Primary research of the importance of ALT testing on the safety of blood donor screening system

Lidong LIU ; Chao LI ; Yu GAO

Chinese Journal of Blood Transfusion.2008;0(07):-.

Objective To analyze the second time blood screening results of ALT deferred donors,and to evaluate the importance of alanine aminotransferase(ALT) testing on the improvement of blood safety.Methods The ALT testing results of 565 360 blood donors from Feb.2006 to Jan.2008 of Shanghai Blood Center were studied retrospectively.The screening results and donation intervals of such donors who delayed their donation just because of their former unqualified ALT level were also analyzed.Results A total of 32 042 donors(5.67%) failed in ALT testing among 565 360 donors.And 3 395 ALT deferred donors participated the second time blood donation,among which 2 205(64.95%) passed the blood screening tests,while the other 1 190(35.05%) failed.Among the 1 190 unqualified blood donors,1 151(33.90%)failed again in ALT testing,and 11(0.32%) in Syphilis,12(0.35%) in HBsAg,7(0.21%) in anti-HCV and 1 in anit-HIV(0.03%).Meanwhile,donors failed both in ALT testing combined with HBsAg,anti-HCV,and anit-HIV sero-converted were 1(0.03%),2(0.06%) and 1(0.03%),respectively.And 72.64% of ALT deferred donors participated the second time blood donation within 6 months.The average donation intervals of donors with qualified ALT level but sero-converted were 140 days(from 24 to 267 days),and those with both unqualified ALT level and sero-converted were 158 days(from 91 to 220 days).Conclusion Before the new methods such as NAT were applied to blood donation screening system,ALT test could prevent the window-period failure of ELISA screening so as to improve the blood safety.

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The influence of two transfusion triggers on middle-and old-aged patients during tumorectomy

Shiguang YANG ; Xiaodong WU ; Jing ZHOU

Chinese Journal of Blood Transfusion.2008;0(08):-.

0.05].Conclusion To the middle-and old-aged GI cancer patients without organ dysfunction,lowering blood transfusion trigger from Hb 100 g/L to Hb 80 g/L during operation can meet the requirement of oxygen,reduce blood transfusion,and do not increase the rates of wound infection or delay wound healing.

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Expression of Toll like receptors on peripheral blood mononuclear cells from patients with idiopathic thrombocytopenic purpura

Xiaoliu LIANG ; Baolong WANG ; Zhimin SHANGGUAN

Chinese Journal of Blood Transfusion.2008;0(08):-.

Objective To study the level of the Toll like-receptor mRNA expression on PBMCs from patients with the idiopathic thrombocytopenic purpura.Methods We performed quantitative real-time reverse transcription-polymerase chain reaction(SYBR Green Ⅰ fluorescent dye combination technique)analyses of PBMCs from 30 idiopathic thrombocytopenic purpura(ITP) patients and 30 healthy subjects,to estimate the relative levels of TLR2,TLR4,TLR6,TLR7 and TLR9 mRNA expression.Results Relative expression levels of TLR2 and TLR7 mRNAs in ITP group(n=30)were significantly higher than those in health control group(n=30)(P0.05).Conclusion Expressions of TLR2 and TLR7 mRNA are up-regulated in patients with ITP,which may play an important role in the pathogenesis of ITP.

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Efficacy of modified PLT additive solution for platelets storage at low temperature(10℃) on rabbit thrombocytopenia model

Xin WANG ; Ronghua SHI ; Jing LI

Chinese Journal of Blood Transfusion.2008;0(08):-.

Objective To evaluate the in vivo efficacy of modified platelet(PLT) additive solution(PAS-ⅢM) with trehalose as a substitute for plasma for the storage of platelet concentrates at low temperature(10℃).Methods Rabbit platelet concentrates were collected and stored in different media and temperature conditions,plasma(22℃),70%PAS-ⅢM/30% plasma(10℃) and plasma/trehalose(-85℃).Platelets in plasma(22℃) on storage day 3,in 70%PAS-ⅢM/30% plasma(10℃) on storage day 3,7,9,and in plasma/trehalose(-85℃) thawed on day 20 were transfused to rabbit thrombocytopenia model.The in vivo recoveries and survivals of the three preserved platelets and fresh platelets were measured and evaluated after transfusion into rabit model of thrombocytopenia.Results The survival of platelets stored in 70% PAS-ⅢM/30% plasma(10℃) on day 9 was lower than that of fresh platelets(P0.05).Recovery and survival of frozen platelets were significant lower than those of the other groups(P

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Knowledge of voluntary blood donation and factors influencing the willingness among rural residents in Anhui

Xiaomei WANG ; Yan DOU ; Zhiqiang XIA

Chinese Journal of Blood Transfusion.2008;0(08):-.

Objective To get a general idea of the knowing level of knowledge and policies related to voluntary blood donation among rural residents,and to explore factors influencing their willingness of voluntary blood donation.Methods Three natural villages were randomly chosen in the north,middle and south of Anhui province as the investigation sites in Jan.2008.Data were collected by interviewing the target people,as well as using a self-designed questionnaire.A total of 724 people were interviewed and 620 valid questionnaires were obtained,with an effective retrieval rate of 85.6%.Results The score of voluntary blood donation knowledge among rural residents was 19.26?3.987,and the score of relevant policy knowledge was 5.85?2.154.There were significant differences in knowledge of both voluntary blood donation and relevant policies among different age groups.Residents with different education levels also differed significantly in knowledge of relevant policies.Logistic analysis indicated that age,education and knowing level of relevant policies were the factors that had influence people's willingness of voluntary blood donation.Conclusion Knowing level of voluntary blood donation knowledge is high among rural residents while the policy knowledge level is low.Willingness of blood donation among the group of age 25 to 34 is obviously higher than that of the group of age 18 to 24;willingness of people with middle school education background is lower than illiterate people;the higher the knowledge level,the higher of people's willingness.

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Molecular polymorphism and the distribution of HLA B-27 subtypes in southern Chinese Han patients with Ankylosing Spondylitis and healthy controls

Zhihui DENG ; Suqing GAO ; Jianqian ZENG

Chinese Journal of Blood Transfusion.2008;0(09):-.

Objective To study the molecular polymorphism and the distribution of HLA-B27 subtypes in southern Chinese Han patients with Ankylosing Spondylitis and healthy controls.Methods A total of 46 samples form southern Chinese Han patients with AS and 80 non-related blood samples from healthy peripheral blood stem cell donors with B27-positive identified by rSSO Lumminex flow array assay were subjected to sequencing analysis of exon 2 ,3 and 4 of HLA-B gene by the sequence-based typing,the purified products of sequencing reaction were electrophoresed on ABI 3730 DNA sequencer and the designation of HLA-B27 allele was accomplished using the Assign3.5 software. The ambiguities and the detected "rare" alleles were confirmed using the PCR-SSP commercial kit. Results In the 46 B27-positive patients with the diagnosis of AS,four alleles,namely B2704,B2705,B2707 and B2724 were determined. The frequencies for these four alleles were 82.98%(39/47),12.77%(6/47),2.13%(1/47) and 2.13%(1/47),respectively. In the 80 B27-positive control individuals,seven B27 related alleles were identified. The frequency for the two dominant subtype B2704 and B2705 were 57.32%(47/82) and 26.83%(22/82),respectively. Both the B2706 and B2707 were observed 5 times with a frequency of 6.10%(5/82),three alleles B2703,B2715 and B2724 were detected only once with a frequency of 1.22%(1/82).Conclusion Our study shows that HLA-B2704 and B2705 were the predominant subtypes in normal healthy controls,however,B2704 was the predominant subtype for the AS group in southern Chinese Han patients.

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Proficiency evaluation of platelet alloantiboby analysis in 42 platelet Immunology laboratories around the world:the 14t~h ISBT Platelet Immunology Workshop and Cooperative Research Project

Weidong SHEN ; Yan ZHOU ; Zhoulin ZHONG ; Guoguang WU

Chinese Journal of Blood Transfusion.2008;0(09):-.

Objective To organize the 14th ISBT Platelet Immunology Workshop and Cooperative Research Project,and proficiency evaluation on the techniques and quality level of platelet alloantiboby analysis will be taken effect for the 42 platelet immunology laboratories around the world. Methods Organized and confirmed by Nanning Institute of Transfusion Medicine,9 quality control samples contained the Human Platelet Antigen (HPA) specificity antibody have been supplied to all the participated laboratories in this project. The participants could use the In-House technology or/and market kits to test these quality control samples. The forms and sheets have been provided for recording of results. Results There were 36 laboratories from 23 countries participated in this Cooperative Research Project,and 35 laboratories have reported their results. The appraised consistent rate of the 9 quality control samples ranges from 20% to 97.14%,the HPA antibody specificities have been showed as anti HPA-1a,anti HPA-1b,anti HPA-3a,anti HPA-3a,anti HPA-3b,anti HPA-5b,anti HPA-5a,anti GPIV and anti HPA-5b+15b,respectively. Among the appraised consistent rate,the anti HPA-3b were the lowest and the anti HPA-5b and anti HPA-5a was the highest. Over 10 technologies of platelet alloantiboby analysis were used by the laboratories. Conclusion This international cooperative research project has successfully made the proficiency evaluation and report on the techniques and quality level of platelet alloantiboby analysis for the 35 international platelet immunology laboratories.

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