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Study on Partial Purification of the Native Human Gamma Interferon (HuIFN-?)

Chinese Journal of Blood Transfusion.1988;0(02):-.

A method for partially purifying the native human gamma interferon (HuIFN-?) was presented. The rude HuIFN-?was concentrated by PEG 4 000, precipitated by (NH_4)_2SO_4 and purified by CM-Sephades C-50(more than 100 times). The titre of HuIFN-?was 1.3X10~5u/ml and its specific activity was 1. 0 X 10~5u/mg protien. Quality control tests were performed and 10 volunteers received intramuscularly the purified HuIFN-?without significant changes in body temperature, pulse rate and blood pressure. It is considered to be suitable for clinical trial.

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Testing the Human Serum for HBsAg by Ultrasensitive Chemiluminescent Photographic Detection-Dot-ELISA Technique

Xiaoyu LIU

Chinese Journal of Blood Transfusion.1988;0(02):-.

The most new ultrasensitive chemiluminescent photographic detectiondot-ELISA (CPD-Dot-ELISA) technique was developed by combining the chemiluminescence technique with Dot-ELISA. The sensitivity of testing for pure HBsAg by CPD-Dot-ELISA was 30 and 60 times higher than by general Dot-ELISA and plate ELISA, respectively. 243 clinical serum specimens had been tested for HBsAg by both plate ELISA and CPD-Dot-ELISA, indicating that of 243 specimens tested for HBsAg by the former, 149 were positive, while of 243 specimens tested for HBsAg by the latter, 194 were positive. Of 149 positive specimmens tested by the former, only Ⅰ wasn't detected by the latter. 14 specimens randomly sampled from the additional 45 positive serum specimens detected by the latter, and 2 serum specimens which proved to be positive by both methods, had then been subject to neutralized test for HBIG, indicating that all 16 mentioned above specimens were positive. The results showed that the CPD-Dot-ELISA technique not only had its high sensitivity, good specificity and repro-ducibility, but also it was simple in manipulation, economically practical and worthy to be widely spread.

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Subgenotype Analysis of Hepatitis C Virus in paid Biood Donors

Zuhu HUANG

Chinese Journal of Blood Transfusion.1988;0(03):-.

The detecting of HCV-RNA and genotyping of HCV in sera of 65 paid blood donors which are positive for anti-HCV were conducted,indicating that 60 of them appeared positive for HCV-RNA,and their genotype of HCV was classified as genotype- I . It was suggested that the genotype- I of HCV could be considered as the predominant infectious strain in this group of paid blood donors. It was found that the endonuclease digestion method of typing based on HCV 5' -noncoding region PCR was better compared with the direct method of typing based on HCV core region PCR in terms of its senstivity,economy and simplicity.

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Investigation on Donors' Blood Lipoids,Blood Composition and Hemorrheological Indexes

Huanhe XIN

Chinese Journal of Blood Transfusion.1988;0(02):-.

A comparison between 70-blood-donor and 70-health-control blood lipoids,blood composition,and hemorrheological indexes by paired analysis method basedon occupation,sex and age was made.Paired T-test showed that though severalindexes of the blood donors reduced remarkably,they were still within the normalphysiological limits,There were no abnormal findings through physical examinationand ECG,indication that according to the general physique and present diet condi-tions of Chinese population.It is safe to donate 300ml blood once at intervals of three months.

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Preliminary Treatment of Separating Albumin from Autoplasma by Heating

Yuanguo ZHOW

Chinese Journal of Blood Transfusion.1988;0(02):-.

A safe and effective method for separating albumin from autoplasma by heatwas introduced.Immunological analysis,showed that such an albumin solutionhad the same identity as human serum albumin.Determination by acetate cellu-lose electrophoresis showed the purity of the albumin was over 90%.Determination by polyacrylamide gel electrophoresis showed that one of thealbumin monomers was over 97%.Detection by double radial immunodiffusionindicated that there were no any new antigens in it.The recovery of the albuminwas more than 80%.

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Preparation of Protein C Deficient Plasma

Xiaopu XIAO

Chinese Journal of Blood Transfusion.1988;0(03):-.

A specific monoclonal antibody(McAb),which could identfy different conformations of protein C(PC) in the presence or absence of Ca24+ .was selected and coupled with Sepharose-4B gel to form an affinity chromatographic column. When fresh plasma passed through this column.its PC would he adsorbed exclusively,and the obtained effuent was PC-deficient plasma (PCDP). The residual PC in the PCDP gained was

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Detection of HLA and Platelet-Associated Antibodies after Blood Transfusion and Its Meaning

Xiuying PAN

Chinese Journal of Blood Transfusion.1988;0(02):-.

A standard microlymphocytotoxicity assay was used to test the sera for HLAantibodies in the patients receiving multitransfusions and to identify their speci-ficity,and at the same time the platelet-associated antibodies were detected.As a result,the dectable rate of HLA antibodies was 39.14% (119/304),of which24.37% (29/119) had specificity;the positive rat platelet-associated antibodieswas 39.83% (47/118).The frequency of positive incidence of HLA antibodiesincreased with the number of transfusions and donors,having a significant differ-ence.The nonhemolytic reaction to transfusion was associated with HLA antibodi-es.The frequency of positive incidence of HLA antibodies was the highest whenthe patients were transfused with concentrated granulocytes.There were takensome measures to prevent and reduce the transfusion reaction.

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Study of Treatment of Refractory Idipathic Thrombocytopentic Purpura with VP-16-binding Platelets

Ziqing DING

Chinese Journal of Blood Transfusion.1988;0(03):-.

A technique of solid-phase pla-telet immunoserological assay wasused to select unsuitable plateletdonors for patients with refractory (?)diopathic thrombocytopenic purpura(RITP).The unsuitable plateletswere used as carriers to bind with VP-16 (Etoposide),and then wesetransfused into5patients with RITP.All the cases were benefited by sucha therapy,and 4 of them were cured.indicating that the therapy has itscurative value in clinical practice.(Original article on page 115)

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ENGLISH ABSTRACTS OF ORIGINAL ARTICLES——Effect of Transfusion with Stored Blood after Combined Radiation-Burn Injury on Immune Reaction of Allo-Skin Grafted Rats

Yongtang YAN

Chinese Journal of Blood Transfusion.1988;0(02):-.

The effect of post-injury transfusion with stored blood on the immune reactionof allo-skin grafted rats was treated,using models of the allo-skin grafted ratswith simple burn injury (15% TBSA) and with combined radiation-burn injury(exposed to 5 Gy systemic irradiation,15% TBSA) respectively.After burninjury and allo-skin graftment,the early cell immune function of the rats signifi-cantly enhanced,the survival time of all the allo-skin grafts was within 7 days.After the rats transfused with stored blood,the degree of their cell immunefunction enhancement was decreased,but there was no significant effect on thesurvival time of allo-skin grafts.However,in the case of allo-skin graftmentsubsequent to combined radiation-burn injury,and then transfusion with storedblood,the multiplying ability of thymocytes and the antibody-producing abilityof splenocytes were depressed significantly and for a long time;the number ofthymocytes,splenocytes,spleen B-cells,T-cells,T_H-cells and Ts-eells were decr-eased rapidly and recovered slowly.And the surviral rate of allo-skin grafts for15 days was 100.0% which was remarkably higher than that of the group subject-ed to simple allo-skin graftment (28.6%,P

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Analysis of TfC Subtypes by Sample Treatment with Rivanol-Ferrous Ammunium Sulfate

Jingxiang CHEN

Chinese Journal of Blood Transfusion.1988;0(03):-.

The sample treatment withRivanol-Ferrous ammumium sulfateis a new procedure which we searchedfor in analyzing Tf C subtypes amongnationalities of South China.Thesample subjected to this treatmentand isoelectric focusing gave clearlyvisible bands,reliable typing resul-ts,and good reproducibility.Onecould determine directly Tf C sub-types and their variants without im-munofixation.The pracedure is easyto perform,economically practical,and can be used in the anthropolongy,population genetics analysis,and inthe TF individual identificationand parent-child relation testing inthe forensic science.

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