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Investigation on Donors' Blood Lipoids,Blood Composition and Hemorrheological Indexes

Chinese Journal of Blood Transfusion.1988;0(02):-.

A comparison between 70-blood-donor and 70-health-control blood lipoids,blood composition,and hemorrheological indexes by paired analysis method basedon occupation,sex and age was made.Paired T-test showed that though severalindexes of the blood donors reduced remarkably,they were still within the normalphysiological limits,There were no abnormal findings through physical examinationand ECG,indication that according to the general physique and present diet condi-tions of Chinese population.It is safe to donate 300ml blood once at intervals of three months.

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Preliminary Treatment of Separating Albumin from Autoplasma by Heating

Yuanguo ZHOW

Chinese Journal of Blood Transfusion.1988;0(02):-.

A safe and effective method for separating albumin from autoplasma by heatwas introduced.Immunological analysis,showed that such an albumin solutionhad the same identity as human serum albumin.Determination by acetate cellu-lose electrophoresis showed the purity of the albumin was over 90%.Determination by polyacrylamide gel electrophoresis showed that one of thealbumin monomers was over 97%.Detection by double radial immunodiffusionindicated that there were no any new antigens in it.The recovery of the albuminwas more than 80%.

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Preparation of Protein C Deficient Plasma

Xiaopu XIAO

Chinese Journal of Blood Transfusion.1988;0(03):-.

A specific monoclonal antibody(McAb),which could identfy different conformations of protein C(PC) in the presence or absence of Ca24+ .was selected and coupled with Sepharose-4B gel to form an affinity chromatographic column. When fresh plasma passed through this column.its PC would he adsorbed exclusively,and the obtained effuent was PC-deficient plasma (PCDP). The residual PC in the PCDP gained was

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Aproaching the Method of Cryopreserving Hemopoietic Stem Cells in Cord Blood

Weiguo ZHU

Chinese Journal of Blood Transfusion.1988;0(02):-.

For the purpose of opening up the future of more widespread application of cord blood,and also for the purpose of setting up the, bank of hemopoietic stem sells in cord blood,an effective method of cryopreserving the cord blood was approached, indicating, that it was appropriate that the time of cryopreserving the hemopoietic stem cells in cord blood at 4℃ should be 2-3 days (the storage of hemopoietic stem cells in cord blood with liquid nitrogen was an effective method of cryopreserving the hemopoietic stem cells in cord blood,with high recovery rate,and longterm cryopreservation without any change in the recovery rate;the effect of storing the hemopoietic stem cells in freezer at-80℃ was similar to that of cryopreserving them with liquid nitrogen,and the former was simple,e-conomic, convenient and practical, and needed no any programmed temperature-dropping apparatus .At the same time, it is convenient to using the stem cells in large scale and to setting up the bank of them. Preserving the whole cord blood can avoid the loss of hemopoietic stem cells in cord blood during the centrifuging process,with the effect of preservation being unchanged.

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Observation on the Hepatic Histology and Serology Changes in HCV-infected Blood Donors

Xuanling SHI

Chinese Journal of Blood Transfusion.1988;0(02):-.

Seven asymptomatic blood donors,in whom the infection with HCV was verified,were selected. The time of their infection with HCV was one and a half years. And the research on the hepatic histology and serology indexes of the donors was performed. The liver tissue samples gotten by needle biopsy of liver ware examined under the common microscope and electron microscope,thereby the abnormal hepatic histology manifestation was found, in all the donors. The samples of seven cases have shown the pathological changes characteristic of early stage of viral hepatitis under microscope. The result of examination under electron microscope confirmed that the infected donors were located in early stage of infection,and showed the low grade hepatic histological lesion. The serological examination showed that all the seven cases had normal ALT level,and most of them were HCV RNA-positive and had genotype-II . It is necessary to conduct follow-up observation on the relationship between the changes of serological indexes and the hepatic lesion.

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Analysis of the Biological Activity of and the Residual SD in the Prothrombin Complex Concentrate after Inactivation of Virus by SD

Rong YU

Chinese Journal of Blood Transfusion.1988;0(02):-.

The biological activity recovery rate of and the residual SD in the 6 batches of prothrombin complex concentrate after inactivation of virus by solvent-detergent was investigated. The average recovery rate of PE activity of prothrombin compound was 87. 83%,the residual Tween 80 was

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In vitro Study on Inhibition of HBV Replication and Expression by Antisense Oligodeoxynucleotides toward HBV Regulation Gene ENⅡ

Peifang DING ; Wensheng SUN ; Chunhong MA

Chinese Journal of Blood Transfusion.1988;0(04):-.

Objective:To screen the high performance,specific and nontoxic anti HBV antisense oligonucleotide fragment.Methods:Taking 2.2.15 cells as cell model,the antisense oligodeoxynucleotides(ASON)toward the HBV regulation genesenhancerⅡ(HBV EFⅡ)were designed and synthesized.The role played by ASON in inhibiting the secretion and expession of HBsAg and HBeAg by the host cells was detected by ELISA method.The effect of ASON on prolifezation and metabolism of the cells was detected by MTT method.Results:The inhibitory rate of HBsAg by ASON was 92% and 75%,respectively,indicating that itsdifference from that of noncomplementary sequence control group(inhibitory rate was 11%)had considerable statistical significance( P 0.05).Conclusion:ENⅡ is one of the most important target oriented sequencial selective regions of research on anti HBV nature of antisense oligonucleotide.

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Cloning and Screening of Mutant Human Hemoglobin Gene(?_(99) ,?_(82) Lys→Cys)

Hao ZHANG ; Quanli WANG ; Qi FENG

Chinese Journal of Blood Transfusion.1988;0(04):-.

Objective:To mutate the codons which code the lysine at loci ? 99 ,? 82 in human hemoglobin gene to the codons which code the cystein.Methods:The objective gene to be mutated was cloned into pAlter Ex2 plasmis and then was mutated with the site directed mutagenesis mediated by oligonucleotide.Results:The sequencing of clones obtained by preliminary screening showed that the anticipated results were reached without any unexpected mutation.Conclusion:The results lay a foundation for the further development of research on expression and purification of recombinated mutant human hemoglobin gene.

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Cloning,Expression of E2 Gene in Hepatitis C Virus Envelope Region and Significance of Anti HCV E2 Detection in Patients with HCV Infection

Xuanling SHI ; Feng CHAO ; Yang JI

Chinese Journal of Blood Transfusion.1988;0(01):-.

Objective To clone and express the E2 gene in envelope region of hepatitis C virus and detect the anti E2 in sera of patients with HCV infection,using the purified E2 Protein.Methods The gene fragment of E2 region was obtained from the sera of patients with HCV infection by random primer reverse transcription and polymerase chain reaction(PCR),and then cloned into procaryotic expression system to express and Purify the protein and detect its antigenicity.Results A segment of E2 region gene with correct code reading frame was obtained,its full length was 984 bp and molecular weight of expressed Protein was 38kD.The detection of the protein performed by ELISA with sera from the patients with HCV infection,testified that it had appropriate antigenicity.Conclusion This experiment can provide the fundamental data for researching on the basic characteristics of HCV envelope protein,nuderstanding the significance of serological diagnosis and designing the anti HCV vassine.

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Serial Expression of Human ?,? globin Genes in Escherichia coli

Hao ZHANG ; Quanli WANG ; Qi FENG

Chinese Journal of Blood Transfusion.1988;0(02):-.

Objective To make an attempt to express serialy the human ?,? globin in Escherichia Coli in order to carry out the research on blood substitute based on the genetic recombination of hemoglobin.Methods The routine molecular biology technology was used.Results The serial genetic fragment of ?,? globins containing the hemoglobin was amplified by PCR.The genetic sequencing showed that there was no unexpected mutation.And then,the serial genes were cloned into the pBV220 expression carrier.Undergoing thermal inducement,the expression product could reach about 20% of total bacterial protein.The expression product assumed the form of inclusion body,and it was vertified by Western Blotting.Conclusion This much can lay the solid foundations for further launching the research on expression and pruification of recombinant human hemoglobin genes.

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