0.05).The volumes of PBSC collected each time in the 3 groups were about 60 ml,and there were(3.67—10.25)?1010 erythrocytes per pack in the ABO major incompatible group,and 22—38 ml of plasma in ABO minor incompatible component.All the PBSC were infused to recipients without the removal of erythrocytes or plasma,and no haemolytic reaction was observed in any recipients and all their hematopoietic functions were re-established.Conclusion Enough PBSC can be harvested by using CS-3000 Plus blood cell separator from ABO incompatible allogeneic donors.By modification of separator parameters,the number of erythrocytes in the collects can be reduced.The collected PBSC can be safely infused into the blood group incompatible recipients without removal of erythrocytes or plasma.