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Integrator complex and its research progress

Lanlan SI ; Hongxing ZHANG ; Gangqiao ZHOU

Military Medical Sciences.2015;(5):398-402. doi:10.7644/j.issn.1674-9960.2015.05.019

The integrator complex is multifunctional and contains at least 12 evolutionarily conserved subunits in hu-mans.It interacts with the C-terminal tail of the largest subunit of RNA-polymeraseⅡ ( RNAPⅡ) to promote 3′-end pro-cessing of small nuclear RNA (snRNA) U1/U2.It also interacts with RNAPⅡ, NELF and Spt5 to regulate NELF-mediated RNAPⅡpause/release and processivity at coding genes .Recently, the integrator complex is also reported to be involved in DNA damage response , dynein recruitment to the nuclear envelope , integrity of Cajal bodies , adipose differentiation , hem-atopoiesis , ciliogenesis , tumorigenesis and generation of viral microRNAs .This review discusses related research progress in the integrator complex .

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Biological characteristics of Ebola virus:research advances

Yi ZHANG ; Yinglong QU ; Zhendong GUO ; Siyan ZHAO ; Yingying FU ; Hongyan ZHAO ; Zhongyi WANG ; Zongzheng ZHAO ; Linna LIU ; Jun QIAN

Military Medical Sciences.2015;(5):386-389. doi:10.7644/j.issn.1674-9960.2015.05.016

Ebola virus disease , which can cause ebola hemorrhagic fever , is a potent zoonotic infectious virus disease . In 2014 , Ebola virus spread across West Africa and it has become a new major threat to global public health .This article summarizes the structural features of Ebola virus , transmission characteristics , interactions ,animal models .

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Multiple linear regression analysis methods for complex random sampled data and their application

Riyang SUN ; Liangping HU

Military Medical Sciences.2015;(5):380-385. doi:10.7644/j.issn.1674-9960.2015.05.015

Objective To study the significance and function of the comprehensive weight in multiple linear regression analysis of complex random sampled data .Methods Based on the concept of Monte Carlo random simulation , two different multiple linear regression analysis procedures in SAS-REG and SURVEYREG were used to perform regression modeling for the same batch of complex random sampled data ( n=6756 ) at different random sampling proportions .The results were compared.Results In the results of the fitting multiple linear regression model when observation weight and sampling weight were considered or not , it was found that the size of the partial regression coefficient , standard error and P value of independent variables varied .Conclusion In complex random sampled data based on different proportions ,especially in regression modeling of stratified random sampling survey information , the multiple linear regression model makes it possible to more accurately and sensitively perform parameter estimates of regression coefficients and statistical prediction of outcome variables if the comprehensive weight of the survey data is incorporated into the statistical analysis .

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Verification of a molecular pathology detection method of sentinel lymph node in breast cancer patients:a comparison between RT-LAMP and GeneSearch

Qiang LIN ; Yi LIU ; Feng LIANG ; Yinhua LIU ; Xuening DUAN ; Xiang WANG ; Tao OUYANG ; Hongchuan JIANG ; Bo LI ; Deqi YANG ; Xiaopeng HAO

Military Medical Sciences.2015;(5):373-376. doi:10.7644/j.issn.1674-9960.2015.05.013

Objective To verify the reliability of our previously established reverse-transcription loop-mediated isother-mal amplification ( RT-LAMP) method for the detection of sentinel lymph nodes metastasis in breast cancer patients .Meth-ods Sentinel lymph nodes of breast cancer patients were analyzed by RT-LAMP and FDA-approved GeneSearch methods respectively, and the consistency of the two methods was assessed with a kappa concordance test.Results One hundred and thirty-four cases of sentinel lymph node samples were collected from seven hospitals in Beijing .Using the GeneSearch assay as the gold standard, the sensitivity, specificity and consistentcy of RT-LAMP were 96.2%(25/26),96.3%(104/108) and 96.3%(129/134), respectively.Statistical analysis showed that the two methods were consistent (Kappa=0.8857, P<0.001).Conclusion RT-LAMP is highly consistent with GeneSearch ,efficient,simple and inexpensive, promising a good prospect of application to intra-operative detection of sentinel lymph nodes metastasis for breast cancer patients.

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Anticonvulsant effect of intranasal midazolam on maximal electroshock seizure and metrazol seizure model in rats

Kai TIAN ; Yuan LUO ; Wanhua LI ; Xin SUI ; Jun YANG ; Aiping ZHENG ; Peifeng WEI ; Yongan WANG

Military Medical Sciences.2015;(5):368-372. doi:10.7644/j.issn.1674-9960.2015.05.012

Objective To evaluate the anticonvulsant effect of midazolam and diazepam when administered nasally on maximal electroshock seizure and metrazol seizure threshold test models .Methods Rats were randomly divided into 7 groups:model group, low-dose, middle-dose and high-dose of midazolam nasal spray groups , diazepam nasal spray in low-dose, middle-dose and high-dose of midazolam nasal spray groups .After the establishment of the maximal electroshock seizure( MES) and metrazol seizure threshold test models ( MST) in rats, the anticonvulsant effects of different doses of midazolam and the clinically used antiepileptic drug diazepam were evaluated and compared .HE staining was used to observe the histopathological changes in the hippocampus , cortex and amygdala in rats .Results Significant anticonvulsant effects were observed on MES and MST in rats pretreated with different dosages of midazolam .In addition , the anticonvul-sant effects of midazolam were stronger than those of diazepam at the same dosage on MES and MST (P<0.05,P<0.01). Histopathological results showed that both midazolam and diazepam could effectively prevent the seizure -induced brain inju-ries, inhibit the increase of microglial cells and the inflammatory cell infiltration in the hippocampus, cortex and amygdala, and reduce the nucleus pycnosis and neuronophagia .Conclusion Midazolam has significantly anticonvulsant and neuropro-tective effects on different seizure models when administered nasally in rats .

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Epidemiological analysis of measles in China between 2005 and 2013

Xiushan ZHANG ; Yicheng WU ; Quan QIAN ; Wanyu LIU ; Wenyi ZHANG ; Shenlong LI

Military Medical Sciences.2015;(5):360-363. doi:10.7644/j.issn.1674-9960.2015.05.010

Objective To analyze the epidemiologic characteristics and epidemic intensity of measles in China between 2005 and 2013 , and to provide evidence for reasonable allocation of health resources and measles elimination in China . Methods Descriptive epidemiological methods were applied to measles surveillance data and demographic information during this study.Results A total of 597 594 cases, including 366 deaths, were reported from all over China , among whom 355 409 were males (59.47%) and 242 185 were females (40.53%).The average annual incidence was 5.07/100 000 and the average annual mortality was 0.0031/100 000.The majority of cases were children under 10 years old, accouning for 64.64%.Conclusion The incidence of measles in China is decreasing .Measles mainly occurrs in spring and there is significant difference between age and gender groups , and obvious regional differences are also found .

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Comparative proteomic analysis of Yersinia pestis 201 and 201△pCD1 strains

Hongsheng LIU ; Jingyu GUO ; Erling FENG ; Xiaoyi WANG ; Hengliang WANG ; Yajun SONG ; Li ZHU

Military Medical Sciences.2015;(5):348-353. doi:10.7644/j.issn.1674-9960.2015.05.007

Objective To explore the potential pathogenesis of Yersinia pestis and provide new clues for vaccine development through comparative proteomic analysis of wild-type and pCD1 cured strain of Yersinia pestis 201.Methods Differentially expressed proteins at 26℃ and 37℃ were separated and identified using two-dimensional electrophoresis coupled with mass spectrometry .Results A total of 24 differently expressed proteins were successfully identified from the samples of bacteria grown at 26℃ and 25 proteins at 37℃.Among these, 7 proteins were encoded by pCD 1 plasmid. Conclusion Through comparative proteomic research, we have found that the abundance of several proteins can be dramatically changed when the large plasmid pCD 1 is missing,suggesting that the plasmid can regulate the expression of many genes located in the chromosome .

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Impact of military stress on levels of interleukin-6 in serum and gastric mucosa of servicemen

Juan GAO ; Guorong ZHENG ; Yan WANG ; Min LI ; Weitian XU ; Yimin XIONG

Military Medical Sciences.2015;(5):345-347. doi:10.7644/j.issn.1674-9960.2015.05.006

Objective To investigate the levels of IL-6 in serum and gastric mucosa of servicemen before and after mili-tary maneuver and their significance .Methods The level of serum IL-6 in 60 servicemen was studied by enzyme-linked immunosorbent assay .The expression of IL-6 in gastric mucosa from the 60 servicemen was detected by immunochemical staining.Results The level of IL-6 in serum of servicemen after military maneuver was significantly higher than that before military maneuver (P<0.01).IL-6 was found to be expressed in the cytoplasm of epithelioglandular cells and also in the cytoplasm of mesenchymal cells .The expression of IL-6 in gastirc mucosa was significantly stronger after military maneuver than before military maneuver (P<0.01).Conclusion IL-6 might be involved in the development of pathological changes in gastric mucosa caused by military stress in troops .Examination of the level of IL-6 in the early stage of military stress might help assess the chance of gastric mucosa lesion and its severity .

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Identification of alternative splicing events in acute myeloid leukemia and chronic myeloid leukemia cells

Fangfang ZHENG ; Tao HE ; Min JIANG

Military Medical Sciences.2015;(5):339-344. doi:10.7644/j.issn.1674-9960.2015.05.005

Objective To reveal the important role of aberrant splicing in cancer and to identify differential expressing isoforms and differential alternative splicing events between acute myeloid leukemia ( AML) and chronic myeloid leukemia (CML).Methods Based on whole transcriptome sequencing of CML (K562) and AML(THP1 and HL-60) cells,TopHat, MATS and Cufflinks were used to identify alternative splicing events and alternative splicing isoforms and analyze differenti -al expression of isoforms and genes .Results A total of 130 genes were identified to be highly expressed in CML and 80 genes in AML cells .Also, 337 differential expressed isoforms and 35 specific alternative splicing events between AML and CML cells were identified.Conclusion There are significant differences in alternative splicing isoforms and events between AML and CML cells.Leukemia-associated alternative splicing events can be used as potential tumor markers or drug targets.

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PM2.5 induces VEGF expression via activation of NF-κB pathway in bronchial epithelial cells

Shasha LIU ; Hongli WANG ; Yongliang HU ; Weiping LI ; Lun SONG

Military Medical Sciences.2015;(5):325-328. doi:10.7644/j.issn.1674-9960.2015.05.002

Objective To investigate the molecular mechanism of vascular endothelial growth factor ( VEGF) expression in bronchial epithelial cells (Beas-2B)induced by particulate matter 2.5(PM2.5).Methods PM2.5 powder was dis-solved in DMEM medium and diluted into five concentrations , 0,12.5,25,50 and 100μg/ml, respectively.The double an-tibiotics ( streptomycin and penicillin ) and FBS were added into the solution to a 2% final concentration of serum system after being treated by ultrasound for 30 minutes.The cultured Beas-2B cells were then treated with different doses of PM2.5.Subsequently, nuclear factor-kappa B(NF-κB) transactivity and the transcriptional activation of vegf gene promot-er were tested by dual-luciferase reporter gene analysis system while phosphorylation of p 65 , expression levels of IκBαand VEGF were detected by Western blotting .Results PM2.5 induced up-regulation of VEGF expression in Beas-2B cells in a dose-dependent manner , accompanied by NF-κB transactivation at the highest level under 100 μg/ml of PM2.5 treatment. Moreover, PM2.5 induced degradation of the repressor protein IκBαand increase in the phosphorylation level of p 65 sub-unit in Beas-2B cells.Knockdown of NF-κB p65 expression significantly inhibited vegf gene promoter transcriptional activa-tion as well as VEGF protein expression in Beas-2B cells induced by PM2.5.Conclusion PM2.5 induces VEGF expres-sion via activation of NF-κB pathway in bronchial epithelial cells .

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