Journal Detail Information

1

Cite

Share

Agriculture bioterrorism:risk and management

Military Medical Sciences.2014;(2):106-108,134. doi:10.7644/j.issn.1674-9960.2014.02.008

In recent years , a series of agriculture biosecurity accidents have occurred ,such as mad cow disease , foot-and-mouth disease and avian influenza , which has aroused worldwide , concern over agriculture bioterrorist attacks .This paper comprehensively analyzes the history and impact of agriculture bioterrorism , the current status of international agricul-ture bioterrorism research , and important issues to be taken into account in future efforts to deal with agriculture bioterrorism .

2

Cite

Share

Study on quality control of the next-generation sequencing data

Sheng LIU ; Zuren FENG ; Shan GAO

Military Medical Sciences.2014;(5):377-380. doi:10.7644/j.issn.1674-9960.2014.05.014

Objective To investigate the quality control of the next-generation sequencing data by analyzing some im-portant problems with quality control in order to provide high-quality data for the downstream data analysis .Method The software Fastq_clean was developed to clean the data and to statistically analyze the quality of the data.Result Using a Chrysanthemum transcriptome dataset , the function of the Fastq_clean software was illustrated .Conclusion Based on the Bioconducter package , the Fastq_clean software can accurately remove low quality nucleotides and Ns , adapter contamina-tion, possible rRNA and virus contamination .Moreover, it can keep the largest possible amount of high-quality data reads.

3

Cite

Share

Clinical analysis of therapies for native valve endocarditis

Li ZHAI ; Yanli HU ; Xiaojin WANG ; Changming XIONG

Military Medical Sciences.2014;(5):374-376. doi:10.7644/j.issn.1674-9960.2014.05.013

Objective To summarize experience in treating patients with native value endocarditis ( NVE) , and discuss the effect of timing of surgery on the infection control , cardiac function recovery and long-term mortality among patients . Methods Eighty NVE cases with antibiotic treatment were recruited between January 2006 and May 2013 .According to the timing of surgery, the subjects were divided into the early surgery group (38 cases) and the late surgery group (42 ca-ses) .The infection-related markers and cardiac function indexes before and after surgery , and 1-year, 3-year and 5-year mortality after surgery were analyzed .Results The infection control and recovery of cardiac function in the early surgery group were better than in the late surgery group (P<0.05).The difference in 3-year and 5-year mortalities between the two groups was significant .Conclusions For NVE patients with routine antibiotics treatment , the surgical effect and long-term survival rate are much better in the early surgery group than in the late surgery group .

4

Cite

Share

Development and application of triphenyltetrazolium chloride (TTC) method for determining the survival of cultured cells

Guowei HE ; Weiguang LI ; Ruixue WANG ; Chenggang ZHANG

Military Medical Sciences.2014;(5):388-391. doi:10.7644/j.issn.1674-9960.2014.05.017

Objective To develop a precise and reliable method to determine mammalian cell survival using the triphe-nyltetrazolium chloride (TTC) solution.Methods Different amounts of TTC solution were added to the cultured cells in order to find the optimal concentration .The correlation between the cell number and the measured optic density value was analyzed.In addition, the TTC method was compared with traditional MTT assay .Results The TTC assay could precisely reveal the viability of cultured cells as well as the MTT method , but the non-specific reaction of the MTT reagent by other antioxidants could result in unreliable overestimation of the cell number .Conclusion The TTC assay can be used to deter-mine the survival of cultured cells more precisely .

5

Cite

Share

Relationships between oncology and military medicine

Hang SU ; Erqing LEI

Military Medical Sciences.2014;(5):384-387. doi:10.7644/j.issn.1674-9960.2014.05.016

Objective To explore the relationship between oncology and military medicine in order to remove theoretical barriers to the development of oncology in military hospitals .Methods The documentary evidence was obtained from cita-tion networks .Results Military hospitals and institutes in China and abroad have conducted studies on oncology , including the long-term survival of Hodgkin′s disease patients in a study of 388 military cases during World War Ⅱ, a study of leuke-mia within the former West Germany Armed Forces , and a study on melanoma in the Armed Forces .Also,some foreign doc-umentation paid special attention to special weapons and equipment , special military operations and special military envi-ronment-related issues of cancer epidemiology , such as cancer following nuclear weapon tests , the association of selected cancers with service in the U .S.military in Vietnam or after the Gulf War .The Military Cancer Institute of the United States published a total of 206 articles between 2001 and 2013 .Conclusion To make closer the relationship between oncol-ogy and military medicine , we should find out more about the relationship between general medicine and military medicine , learn foreign from experience on development of oncology , and explore the military medical value of oncology .

6

Cite

Share

Co-effects of extremely low frequency electromagnetic field (ELF-EMF) and temper-ature on hsp22 and hsp26 expression in Drosophila melanogaster

Jing ZHANG ; Ziyan ZHANG ; Chuanjun YANG ; Peng CAI

Military Medical Sciences.2014;(5):321-326. doi:10.7644/j.issn.1674-9960.2014.05.001

Objective To study the combined effect of extremely low frequency electromagnetic fields ( ELF-EMF ) exposure (50 Hz and 3 mT) and high temperature exposure on hsp22 and hsp26 expression in Drosophila melanogaster. Methods Under the conditions of 50 Hz and 3 mT ELF-EMF,the male and female D.melanogaster was separately exposed for 2 h at 25℃, 27.5℃, 30℃, 32.5℃,and 35℃, respectively.The death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster were detected after exposure;or at 25℃and 35℃at 2 h, 4 h, 8 h, and 24 h, respectively.The hsp22 and hsp26 expression levels of D.melanogaster were tested after exposure .Results After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, temperature had a significant effect (P<0.01) on the death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster, but ELF-EMF had no significant effect (P>0.05).After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, the hsp22 and hsp26 expression levels of D.melanogaster were significantly impacted(P<0.01) by gender, temperature, ELF-EMF and exposure time, and tem-perature was the most important factor .Gender, temperature, ELF-EMF and exposure time had some interaction with the results.Conclusion ELF-EMF can influence the expression levels of hsp22 and hsp26.ELF-EMF exposure contributes to heat tolerance in D.melanogaster through accelerated expression of hsp22 and hsp26.

7

Cite

Share

Advances in research on molecular design of antimicrobial peptides

Xingdong LIU ; Qingbin MENG ; Qingguo MENG

Military Medical Sciences.2014;(5):396-399. doi:10.7644/j.issn.1674-9960.2014.05.019

Antimicrobial peptides ( AMPs) are a kind of micromolecular peptides with antibacterial , antivirus and anti-tumor activities.Unlike conventional antibiotics , acquisition of resistance by a sensitive microbial strain against AMPs is surprisingly improbable .AMPs are not widely used clinically due to their toxicity , susceptibility to proteolysis and high manufacturing cost .Researchers have obtained some AMPs with high activity and low toxicity by structure modifications . The molecular design of AMPs as well as their development in the future are reviewed .

8

Cite

Share

Effect of hyperbaric oxygen on patients with severe traumatic brain injury and on intracranial hemodynamics

Fei YAN

Military Medical Sciences.2014;(5):371-373. doi:10.7644/j.issn.1674-9960.2014.05.012

Objective To investigate the effect of hyperbaric oxygen on cerebral hemodynamics and intracranial pressure(ICP)in patients with severe traumatic brain injury (SBI).Methods A retrospective analysis was conduccted .82 cases of SBI patients treated in our hospital from January 2013 to January 2014 were randomly and evenly divided into two groups.Control group received conventional treatment while observation group was treated with continuous hyperbaric oxygen therapy.Intracranial hemodynamic parameters before and after treatment were determined and compared between the two groups.Results Peak systolic velocity ( Vs) and mean flow velocity ( Vm) of the middle cerebral artery ( MCA) were faster in hyperbaric oxygen group than in control group (P<0.05); the pulsatility index was lower than in control group (P<0.05);intracranial pressure (ICP) values (11.20 ±3.39)mmHg were significantly lower in observation group after hyperbaric oxygen therapy than in control group (15.24 ±3.13)mmHg.The difference was statistically significant (P<0.05), and the total effective rate was 85.37% vs 68.29% in control group(P<0.05).Conclusion Early hyperbaric oxygen therapy can significantly enhance SBI patients immune function and the ability to fight against infections in critically ill patients, therefore improving patients outcomes and overall quality of life .

9

Cite

Share

Advances in biomarkers of oxidative stress under sulfur mustard exposure

Yizhou MEI ; Xiaorui ZHANG ; Wenxia ZHOU ; Yongxiang ZHANG

Military Medical Sciences.2014;(5):392-395. doi:10.7644/j.issn.1674-9960.2014.05.000

Sulfur mustard [bis (2-chloroethyl) sulfide, sulfur mustard, SM] is considered a powerful chemical warfare agent.There is still no specific antidote due to its complex toxicological mechanism .An intimate knowledge of the toxic mechanisms and pathophysiological changes is important to the treatment of sulfur mustard injury .Oxidative stress is one of the most important pathophysiological processes involved in the toxic effect of sulfur mustard .The study of oxidative stress biomarkers induced by sulfur mustard can help to reveal the role of oxidative stress in sulfur mustard intoxication , which may contribute to better understanding of the toxic mechanism and development of therapeutic measures after sulfur mustard exposure.The research advances in oxidative stress biomarkers in sulfur mustard intoxication are reviewed .

10

Cite

Share

Analysis of cell death induced by xCT deficiency based on differential proteomics technology

Jianli QI ; Yang LI ; Liping ZHAO ; Haixuan QIAO

Military Medical Sciences.2014;(5):327-332. doi:10.7644/j.issn.1674-9960.2014.05.002

Objective To identify the mechanisms underlying xCT deficiency by high-resolution proteomic analysis of differential protein expression in Sut and wild melanocytes .Methods The proteins,extracted from Sut and wild melano-cytes,were analyzed by two dimensional electrophoresis and PDQuest software .Subsequent MALDI-TOF mass spectrometry analysis was carried out .The protein identification was based on peptide mass fingerprint combined with the pI and the rela -tive molecular mass ( Mr) .Sequence coverage was performed with the Peptldent software on the NCBnlm website .Also,the autophagy marker protein LC3-Ⅱ, and the autophagic cell death marker protein Beclin 1 were detected by Western blotting . Results and Conclusion Twenty apparently upregulated or downregulated proteins were identified .Strikingly, important modifications in regulators of trafficking and organization of vesicles and autophagy ( Anxa3; Hist 1h2bk, NDRG1, and CaM) and in regulators of invasion and metastasis of carcinoma (S100A-4;S100A-6 and vimentin)are likely to account for dysfunctions in cell viability and cell-extracellular adhesion .These results indicate that the proteins regulating autophagy , vesicles trafficking and MAP kinase related pathways are activated and play a role in xCT-deficiency .

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share