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Xueqiong YANG ; Meihong LI ; Yabin YANG ; Zhongtao DING

Chinese Traditional and Herbal Drugs.2005;36(6):808-810.

Objective To investigate the chemical costituents from Drymaria diandra. Methods Compounds were separated and purified by repeated column chromatographies on macroporous resin D101,silica gel, and RP-18.Two compounds were identified by spectral analysis. Results Two compounds were isolated from D. diandra. Theirs structures were identified as 6-carboxymethy1-5,7,4'-trihydroxyflavone (Ⅰ) and l-O-β-D-glucopyranosyl-(2S,3R,4E,8E)-Z-N-(2'-hydroxypalmitoyl) octadecasphinga-4,8-dienine(soya cerebroside Ⅰ,Ⅱ). Conclusion Compound I is a new compound. Compound Ⅱ is obtained from this plant for the frist time.

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Chemical constituents from root of Glycyrrhiza uralensis

Xumin ZHU ; Yingtong DI ; Shulin PENG ; Mingkui WANG ; Lisheng DING

Chinese Traditional and Herbal Drugs.2003;34(3):198-201.

Object To investigate the chemical constituents from the roots of Glycyrrhiza uralensis Fisch. Methods The constituents were isolated on normal and reversed silica gel column chromatography and their structures were identified by spectral evidence. Results A new oleanane-type triterpenoid saponin and twelve known compounds, including two triterpenoid saponins, two cumarins and eight flavonoids, were isolated. Conclusion The new compound was elucidated as 3-O-[β-D-(6-methyl) glucuronopyranosyl (1→2)-D-glucuronopyranosyl]-24-hydroxy-glabrolide on the basis of ESI-MS, 1HNMR,13CNMR, HMQC and HMBC spectral evidence.

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Stellera chamaejasme induced apoptosis of HL-60 cells and regulated expression of bcl-2 protein in SGC-7901 cells

Zhengping JIA ; Yanguang WANG ; Junjie FAN ; Jingwen XIE ; Liting XU ; Sheng LIU

Chinese Traditional and Herbal Drugs.2001;32(12):1097-1101.

Object To explore the antitumor mechanism of Stellera chamaejasme Linn.(SC).Methods SC containing-serum(SCCS)was derived from mice pretreated with different doses of SC.Cultured human leukemia HL-60 and human gastric adenocarcinoma SGC-7901 cells were used.Inhibition of proliferation was measured using MTT assay.Morphological assessment of apoptosis was performed with fluorescence microscope.DNA fragmentation was assessed by agarose gel electrophoresis and flow cytometry.Expression of bcl-2 protein was measured with immunohistochemistry.Results Exposure of exponentially growing HL-60 cells to mice serum containing 10% SC(pretreated with SC3,6, and 12 g/kg)for 48h resulted in growth inhibition in a dose-dependent manner.Typical morphological changes of apoptosis and DNA fragmentation in HL-60 cells were induced."Apobodies'in the apoptotic cells were observed,'ladder"pattern of agarose gel electrophoresis of DNA from 11.7% to 57.4%.Treatment with SC containing serum decreased the percentage of SGC-7901 cell of bcl-2 protein positive expression from 78.3% to 32.9%.Conclusion SC could induce apoptosis of HL-60 cells and decrease the expression of bcl-2 protein of gastric adenocarcinoma SGC-7901 cells.

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Antiinflammatory Effects of Alcohol Extract of Daoguaniou (Caesalpinia sepiaria var. pubescens)

Le LI ; Feier ZHUANG ; Zengli LI

Chinese Traditional and Herbal Drugs.1994;0(06):-.

An tiinflammatory effects of alcohol extract of Caesalpinia sepiarla Roxb. var. pubescent Tang et wang were shown by ip iujection. The extract significantly decreased xylene induced swelling of mice ear; carrageenin produced edema of hind paw in rats, and decreased volume of pleural exudate on carrageenin-induced acute pleurisy of rats. It also inhibited egg white induced arthritis iu rats.LD50 value is 303.7mg/kg by ip in mice, confidence limit of 95% is 296.5 ? 189.4mg/kg.

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Study on the Identifi－Cation of Bile－Cyst of Bear by Disc Gel Electrophoresis

Zhaoqing ZHONG ; Zhongzhi QIAN

Chinese Traditional and Herbal Drugs.1994;0(07):-.

Disc polyacrylamide gel clectrophorsis was performed to detect water soluble proteins of the bile－cysts of bear, pig, cow and sheep. The bands of electrophorsis were analysed by means of gel clecrophoresis scan. The results showed that different bile－cysts have different bands.Thus, this method can be used to identify the bile－cyst of bear.

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Chemical Studies on Peptide-Polysaccharides of Lingzhi (Ganoderma lucidum )

Yunqing HE ; Rongzhi LI ; Tingwei CAI

Chinese Traditional and Herbal Drugs.1994;0(08):-.

Two glycan peptides, GLSP2 and GLSP3 were obtained from the hot-water extract of thefruit-body of Ganoderma lucidum. Gel chromatography and HPLC showed that they are bothhomogeueous polysaccharides,with molecular weights of 12800 aud 14100 respectively. Throughthe procedures of total acidic hydrolysis, periodate oxidation, Smith degradation, and spectraldetermination, GLSP2 proved to be a glycan peptide with ? (1→3 ) (1→6 ) and (1→4 )linkage, 26.6% of which is peptide; while GLSP3 proved to be a glycan peptide with ?- (1→6 ) and (1→4 ) linkage, aud the sugar residue ratio in these two bonds is 1∶1. The chain ofGLSP3 has branches, and peptide takes 12.3% 0f it. Basic ?-elimination reaction iudicatedthat the sugar residues in the two glycan peptides are linked with O-bond to serine and threonine on the peptide chain.

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