Objective To investigate the non-alkaloid constituent of Hippeastrun vittatum (Amaryllidaceae). Methods Solvent extraction and column chromatography were used to isolate the non-alkaloid constituents, and physicochemical constants and spectroscopic analysis were employed for structural elucidation. Results Five glycosphingosilipids were isolated, and their structures were elucidated to be (2S,3R, 4E, 8Z)-2-[(2R-2-hydroxyhexadecanoyl) amido ]-4, 8-octadecadiene-1, 3-diol 1-O-β-D-glucopyranoside ( Ⅰ ), (2S, 3R, 4E, 8E)-2-[(2R-2-hydroxyhexadecanoyl) amido]-4, 8-octadecadiene-1, 3-diol 1O-β-D-glucopyranoside ( Ⅱ ), (2S, 3R, 4E, 8Z)-2-[(2R-2-hydroxyoctadecanoyl) amido]-4, 8-octadecadiene-1, 3-diol 1-O-β-D-glucopyranoside (Ⅲ), (2S, 3R, 4E, 8E)-2-[(2R-2-hydroxyoctadecanoyl)amido]4, 8-octadecadiene-1, 3-diol 1-O-β-D-glucopyranoside ( Ⅳ ), (2S, 3R, 4E, 8Z)-2-[(2R-2-hydroxyeicosadecanoyl) amido]-4, 8-octadecadiene-1, 3-diol 1-O-β-D-glucopyranoside (Ⅴ), respectively. Conclusion They are all isolated from the fresh bulbs of H. vittatum for the first time.