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Daidzein and Genistein produced by a marine Micromonospora carbonacea FIM 02-635

Chinese Journal of Marine Drugs.2007;26(1):8-12.

Objective To study the active secondary metabolites from marine microorganism FIM02-635. Methods The producing strain was identified by taxonomical and phylogenetic studies. Two compounds FW635I1 and FW635I2 with immunosuppressive activities were extracted by organic solvents from the culture broth and purified by silica gel column chromatography and high speed counter current chromatography. The structures of the two compounds were determined by physico-chemical properties and spectral analyses,the biological activities were assayed in vitro. Results and Conclusion The producing strain was named as Micromonospora carbonacea FIM 02-635. Two compounds FW635I1 and FW635I2 were determined to be isoflavone Daidzein and Genistein, respectively, showed immunosuppressive and antitumor activities, but not antimicrobial activities.

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Antiviral activity and its antiviral mechanism of a sulfated polysaccharide from Sargassum patens against respiratory syncytial virus (RSV)

Chinese Journal of Marine Drugs.2006;25(1):32-38.

Objective To detect the antiviral effect and its antiviral mechanism of the sulfated polysaccharide SP2 isolated from the brown alga Sargassum patens C. Ag. against RSV.Methods The antiviral activity,cytotoxicity and its possible antiviral mechanism were assayed by cytopathogenic effect (CPE) inhibition assay,MTT assay and plaque reduction assay. Reduction assay when it was added during virus adsorption. It did not show any antiviral activity against influenza A and B viruses and parainfluenza type 3 virus up to the concentration of treatment of cells with SP2 did not protect the cells from RSV infection. This polysaccharide addition assay showed that when SP2 was present only during virus adsorption phase at 37℃, it could significantly inhibit RSV-induced plaque formation. Conclusions SP2 might be a potent substance for treating RSV infection. The mode of action of SP2 might depend on inhibiting RSV attachment to host cells.

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A new triterpene glycoside from the sea cucumber Colochirous anceps

Yongjuan ZHANG ; Xuejun Lü ; Yanghua YI

Chinese Journal of Marine Drugs.2005;24(2):13-17.

Objective To search for triterpene glycoside with new structure. Methods We studied the sea cucumber Colochirous anceps belonging to the family cucumariidea with many chromatography methods and found a new sulfated triterpene glycoside, which structure was established by a combination of spectroscopic analysis (IR, UV, ESI-MS and 2D-NMR), chemical transformations and other chemical evidence. Results and Conclusion The new sulfated triterpene glycoside was isolated and named Colochiroside A (1) .

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Chemical composition of Porphyra haitanensis (Rhodophyta, Bangiales) in China

Chengliang XIE

Chinese Journal of Marine Drugs.2009;28(1):29-35.

Objective To analyze the chemical composition of Porphyra haitanensis cultivated in Fujian Province.Methods The contents of crude protein,carbohydrate,crude fat,ash,free amino acids,inorganic elements,chlorophyll a,carotenoid,phycoerythrin and phycocyanin of dried P.haitanensis were determined by routine analysis methods.Results It showed the contents of crude protein,carbohydrate,crude fat and ash of dried P.haitanensis were 34.88%～37.21%,46.86%～49.27%,1.68%～2.09%and 8.75%～9.92%.The contents of chlorophyll a,carotenoid,phycoerythrin and phycocyanin in P.haitanensis were 60 1～646,120～130,1289～1802,1255～1743mg(100g)-1.P.haitanensis is rich in free amino acids and inorganic elements,i.e.K,Na,Mg,Fe,P and Zn,but very little heavy metals,i.e.Cu,Cd and Cr.Conclusions The P.haitanensis was a kind of health food,which was in high content of protein,low content of fat and rich of inorganic elements.

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Effect of carboxymethyl chitosan on sodium currents in isolated ventricular myocytes of rats

Hongguang NIE ; Lei CHEN ; Minjie WEI ; Jinming LI

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To investigate the effect of carboxymethyl chitosan on sodium currents (I_(Na)) in single ventricular myocyte of rat,and to explore the mechanism of carboxymethyl chitosan at the ionic channel level.Methods Single ventricular myocyte of rat was isolated with enzymatic dissociation technique.Whole-cell patch clamp technique was used.The stimulating protocol was:holding potential—120 mV,command potential—80 mV to +20 mV,step potential 10 mV,duration 50 ms,stimulating interval 2 s.Results Carboxymethyl chitosan inhibited sodium channel in a dose-dependent manner.When holding potential was—120mV,carboxymethyl chitosan(0.1%,0.2%,0.3%) decreased the peak amplitude of I_(Na) density to 57.4%?6.7%,40.9%?5.4%and 16.1%?4.3%(P

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Antitumor effects of the polysaccharides from Gloiopeltis furcata on H_(22) tumor bearing mice

Jie YU ; Meizhen CHEN ; Zhaocheng XU ; Shichao CUI ; Wu TANG

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To study the antitumor effect and the mechanism of the polysaccharide from Gloipeltis furcata on H_(22),tumor bearing mice.Methods The polysaccharides from Gloipeltis furcata were administered by oral route in mice bearing H_(22) tumor.The treatments lasted for 7 days.The inhibition rate against H_(22) tumor and the indices of thymus, spleen and liver were measured.In addition,the levels of antibodies against H_(22) tumor and GPT in serum,and GPT,GOT,MDA and SOD in liver were measured using commercially available kits.Results The administration of the polysaccharides from Gloipeltis furcata(200 mg?kg~(-1)?d~(-1) and 400 mg?kg~(-1)?d~(-1) ) for 7 days,the inhibition rates of H_(22) tumor were 35.64%(P

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Protective effects of astaxanthin on the oxidative damage of plasma membrane induced by hydrogen peroxide

Xiuming CAO

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To test the hypothesis that astaxanthin may protect the plasma membrane from oxidative damage induced by hydrogen peroxide(H_2O_2).Methods Using H_2O_2- induced red blood cells damage as the model of oxidative damage of the plasma membrane,the protective effect of astaxanthin(1.0?10~(-7)、1.0?10~(-6)、1.0?10~(-5)mol?L~(-1)) on oxidated erythrocyte ghost was observed by the measurement of the haemolysis of the erythrocyte,the morphology of the erythrocyte,the content of malonidial aldehyde(MDA),the enzymatic activity of the superoxide dismutase(SOD),the membrane fluidity and the blocking degree.Results The rate of hematolysis and erythrocyte MDA content were increased significantly and SOD activity,membrane fluidity and blocking degree of the erythrocyte were decreased owing to the use of H_2O_2.When the astaxanthin was pretreated,the rate of hematolysis and MDA content of erythrocyte were decelerated obviously and the erythrocyte SOD activity,erythrocyte membrane fluidity and blocking degree were accelerated obviously.Conclusion Astaxanthin showed protective effect on the oxidative damage of plasma membrane induced by H_2O_2, and the possible mechanism was relevant with its antioxidation.

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Study on the antioxidant activity of extracts from Enteromorpha prolifera

Qian SHI ; Junhui CHEN

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To investigate the antioxidant activity of Enteromorpha prolifera by ABTS radical scavenging assay in vitro and provide scientific basis for exploitation of Enteromorpha prolifera.Methods The antioxidant activities of extracts by different solvents from Enteromorpha prolifera and water extracts from different Enteromorpha prolifera samples were investigated by ABTS radical scavenging assay.Moreover,the antioxidant ability of the water extract from Enteromorpha prolifera was evaluated by using caffeic acid and ascorbic acid as positive control standards.Results The water extract from Enteromorpha prolifera provided the highest radical scavenging activity,and the antioxidant capability of 1mL of water extract from Enteromorpha prolifera was equivalent to 1mL of 2.55 10~(-3) mg?mL~(-1) caffeic acid or 1mL of 2.89?10~(-3)mg?mL~(-1) ascorbic acid.However,the extraction methods,dry sample or not and different kinds of Enteromorpha prolifera samples had certain influence on the antioxidant activity of water extract from Enteromorpha prolifera. Conclusion There are abundant antioxidant compounds present in the aqueous extract of Enteromorpha prolifera,and it can be exploited as latent oxidation inhibitor.

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Therapeutic effects of Okam on asthmatic mice

Rongjun LIN ; Junxing GAO ; Xiaomei LIU ; Ning XIE

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To investigate the therapeutic effects and mechanism of Okam on asthmatic mice.Methods The mice model of asthma was established with the egg albumen sensitization, and were treated with low and large doses of Okam for four weeks.The pathological changes of lung tissues were observed.Immunohistochemistry was used to determine the contents of matrix metalloproteinases-9(MMP-9) and tissue inhibitor Metalloproteinase-1 (TIMP-1) in the lung tissues.Results Gasp and inflammation in the lung tissues were significantly relieved in the test groups than that in the model group.MMP-9,TIMP-1 and MMP- 9/TIMP-1 ratios were significantly lowered in the test group than that in the model group. Conclusion The marine drug of Okam shows therapeutic effects on mice with asthma,which may be the result of reduced contents of MMP-9 and TIMP-1 in the lung tissues and the correction of the imbalanced MMP-9/TIMP-1 ratio.Okam is likely to be a new choice for the treatment of asthma.

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Optimal fermentation conditions for the production of antitumor active substances by marine bacteria HGX5 and preliminary study on their physicochemical properties

Lei GUO

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To investigate the optimal fermentation conditions for the production of antitumor active substances by marine bacteria HGX5 and preliminary study on their physicochemical properties.Methods Six factors including glucose,peptone,yeast extract,culture temperature,medium pH and agitation were investigated by Plackett-Burman Design, and the important factors including culture temperature,glucose and peptone were optimized by using response surface methodology(RSM).Results The optimal fermentation conditions were culture temperature 35℃,glucose 2.0g?L~(-1) and peptone 9.3g?L~(-1).The antitumor active substances showed better heat and pH stabilities via physicochemical experiments. Conclusion The adopted approach based on Plackett-Burman design and RSM was fit for the fermentation of this strain.

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