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Studies on the secondary metabolites from marine fungus 09-1-1-1

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To search for novel antifungal structures produced by marine fungus.Methods Using the Pyricularia oryzae (P.oryzaae) bioassay method to screen marine fungi led to the isolation of marine fungus 09-1-1-1.Active metabolites were isolated from the broth of the marine fungus 09-1-1-1.Results Two compounds were extracted from the mycelium and their structures were elucidated by various spectroscopic methods as 3a,12c-dihydro-8-hydroxy-6-methoxy-7H-furo\furo\xanthen-7-one(Ⅰ,sterigmatocystin) and 1,3,6,8-tetrahydroxy-2-(1-hydroxyhexyl)anthraquinone(Ⅱ,averantin).Conclusion The P.oryzae bioassay was a cheap,quick and convenient method for the screening of bioactive substances from marine fungi.Compound Ⅱ showed potent inhibitory activity to the mycelia of P.oryzae.The MIC of compound Ⅱagainst P.oryzae was 1.6 ?g?mL~(-1).

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Study on enzymic hydrolytic conditions of skin collagen from Oreochromis niloticus

Mingyong ZENG

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To study the single and combined-enzymic hydrolysis conditions of Oreochromis niloticus skin collagen.Methods Based on the inhibitory activities of six kinds of single enzymic hydrolysates to angiotensin converting enzyme(ACE),bromelain and alcalase(2.4L) were selected as combined-enzyme.The optimal hydrolytic conditions of the skin collagen with the two enzymes were determined by orthogonal test L_(16)(4~(5)).Results and Conclusion The results showed that the optimal hydrolytic conditions of bromelain and alcalase 2.4L were temperature 45℃,pH4.5,E/S 4000U?g~(-1),time 4h,concentration of substrate 6%;and temperature 55℃,pH7.5,E/S 6000U?g~(-1),time 2h,concentration of substrate 4%,respectively.Furthermore,hydrolytic effect would be better when using bromelain in advance,followed by alcalase 2.4L.The hydrolytic degree of the hydrolysates prepared under the provided technology was 30.43%,the ACE inhibitory rate would be as high as(68.65%) in vitro.

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A new natural ceramide N-octadecanoyl-phytosphingosine from soft coral Sinularia sp.

Ruolin YANG

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To find new compound from soft corals.Methods The chloroform portion of the soft coral Sinularia sp.was subjected to silica gel to give ceramide(Ⅰ).Results Structure of compound(Ⅰ) was identified as 2S,3S,4R-N-octadecanoyl-phytosphingosine on the bases of spectroscopic(IR,MS,NMR) and chemical analyses.Conclusion Compound(Ⅰ) was a new natural ceramide and its IR, NMR data were reported here for the first time.

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Progress in the study of marine malabaricane triterpenes

Songzhi DENG

Chinese Journal of Marine Drugs.1994;0(01):-.

The developments in the study of marine malabaricane triterpenes were reviewed in this paper. The relationships between chemical structures and biological activities of marine malabaricane triterpenes were also described.

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Study on the protective effect of polypeptide from Chlamys farreri(PCF) against murine thymocytes apoptosis induced by UVB radiation

Lihua FANG

Chinese Journal of Marine Drugs.1994;0(02):-.

Objective To study the protective effect of PCF against murine thymocytes apoptosis induced by UVB irradiation.Methods The irradiation damage model of thymocytes caused by UVB was established.Cellular ultrastructuralalterations were observed with TEM.The expressions of P53,Bax and Bcl-2 proteins were examined by immunocytochemical technique. The p38 mRNA gene expression was also determined with the technique of in situ hybridization.Results Apoptotic thymocytes were observed after UVB irradiation.The ultrastructural damages of the cells induced by UVB irradiation were reduced by the treatment of PCF.PCF also raised the expression of Bcl-2 gene and decreased the expression of P53,Bax.The mRNA expressions of p38 were decreased in PCF groups.Conclusion PCF showed significant protective effects on UVB-irradiated murine thymocytes in vitro.

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lnfluences of Rhodospirillaceae on immune function of mice

Jianmin HOU ; Jin LAN

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To investigate the influences of Rhodospirillaceae on immune function of mice.Methods Four tests were performed in mice after 4 w medication with different doses of Rhodospirillaceae ig,including:1.The transformation of spleen lymphocyte(MTT method);2.The phagocytosis of macrophagocyte(semi-vivo method);3.The value of HC50; 4.The activity of NK cell(LDH method).Results1.The transformation of spleen lymphocyte;2.The phagocytosis of macrophagocyte,the Value of HC50 and the activity of NK cell in test groups were all raised,the differences were significant and very significant when compared with the control group.Couclusions Rhodospirillaceae could enhance the cellular and humoral immunity of mice.

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Effect of C-phycocyanin from Spirulina platensis on erythropoiesis in mice

Chengwu ZHANG

Chinese Journal of Marine Drugs.1994;0(02):-.

To investigate the effects of C-phycocyanin(C-PC) from Spirulina platensis on erythropoiesis in mice.The colony forming uniterythroid(CFU-E) and burst forming unit-erythroid(BFU-E) were determined using micro-methycellulose culture method in vitro.The erythropoietin(EPO)-like activity of C-PC was examined using the technique of CFU-E culture of fetal liver cells in mice in vitro.Anemic mice models were established by ~(60)Co ?-ray irradiation(5Gy) and intra-peritoneal injected with benzohyarazine hydrochloride.After normal mice were intra-peritoneal injected with C-PC (50 mg?kg~(-1)) for 5d,C-PC provided the only increase in the numbers of CFU-E and BFU-E-derived colonies.C-PC exhibited higher EPO-like activity.12.5 ng of C-PC could match with EPO 1.06U.The specific activity of C-PC was 84,800 U?mg~(-1) C-PC.When anemic mice were intra-peritoneal injected with C-PC for 5d,on d10 the leukocyte,erythrocyte and hemoglobin were significant increased.

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OYSTER EXTRACT CAPSULES IN THE TREATMENT OF HYPERLIPEMIA: A CLINICAL OBSERVATION OF 20 CASES

Yuanmei MIAO

Chinese Journal of Marine Drugs.1994;0(01):-.

Oyster Extrt Capsules(OEC) ,a natural marine preparation from oyster flesh, are used for a lot of diseases, such as cerebrovascular diseases,hepatitis, tumors,etc. In this presentation a clinieal observation on 20 cases of hyperlipemia is reported ,The results showed that OEC had a marked efficacy in treating hyperlipemia.

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APPLICATION OF ALGINIC GLUE IN TOPICAL PREPARATION I. STUDY OF THE FILM-FORMING MATERIAL

Guoqing SHEN ; Ge LU ; Xunquan LIU ; Zhijang CHEN ; Lei CHEN

Chinese Journal of Marine Drugs.1994;0(01):-.

The pigmentum was prepared by using alginic glue prepared by the authors as the film - forming material. It was compared with pigmentum of Alg. Na and PVA in antibacterial activity, stability, film -forming and drug -release. The results showed that alginic glue was better than both Alg. Na and PVA. This pigmentum is an effective treatment for exudative dermatosis .

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CHEMICAL STUDIES ON THE GORGONIAN ISIS MINORBRACHLASTA

Xiaoshuang YANG ; Jingyu SU

Chinese Journal of Marine Drugs.1994;0(01):-.

The chemical constituents of the gorgonian Isis minorbrachlasta have been studied for the first time. Th?marine specimen was collected off the Nansha Islands in the South China Sea . On the basis of spectral correlation (1H NMR,13C NMR,IR)four known compounds:gorgosterol,22 - epi-hippuristanol, ceramide and batyl alcohol, were indentified.

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