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Extraction and purification of the polysaccharides from Dunaliella salina(PDS) residue

Chinese Journal of Marine Drugs.2001;0(05):-.

Objective To study the extraction and purification of polysaccharides from D.salina residue after extraction of ?-carotene.Methods The crude polysaccharide was extracted by hot basic water,and then was isolated and purified by the DEAE-cellulose and Sepharose-4B.Results After having been purified by DEAE-cellulose chromatography,three components named PDS_(1),PDS_(2),PDS_(3)were obtained.Each component was divided into two components by Sepharose-4B chromatography,then six components were obtained.Conclusion The six components were identified to be homogeneous polysaccharide-protein complexes which were composed of sulfate radical and uronic acid.

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Studies on chemical constituents of the sea cucumber Holothuria fuscocinerea

Shuyu ZHANG

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To study the chemical constituents of the sea cucumber Holothuria fuscocinerea collected from the South China Sea.Methods The compounds were isolated by Pyricularia oryzae bioassay-guided fractionation method in combination with extraction and partition as well as multi-chromatography.Their structures were determined on the basis of spectral analysis and chemical evidence.Results Three triterpene glycosides were isolated from the n-BuOH extract and identified as: pervicoside C(Ⅰ),holothurin A(Ⅱ) and DS-holoturin B(Ⅲ);Four compounds [uracil(Ⅳ),thymine(Ⅴ),thymidine(Ⅵ) and 2'-deoxyuridine(Ⅶ)] were obtained from the CHCl_(3) extract.Conclusion All the compounds were isolated from this sea cucumber for the first time while Compound Ⅰ was first obtained as pure compound.Compound Ⅰ and Ⅱ were active compounds causing morphological abnormality of P.oryzae mycelia and exhibited cytotoxicity against two cancer cell lines.

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Antitumor components from sponge-derived fungus Penicillium auratiogriseum Sp-19

Zhihong XIN

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To isolate and identify the antitumor bioactive components from the fermentation of the fungus Penicillium auratiogriseum Sp-19 derived from sponge Mycale plumose.Methods The antitumor components were isolated by bioassay-guided fractionation and using solvent extraction,silica gel column chromatography and preparative HPLC.Their structures were established by physicochemical properties and spectral analyses.The cytotoxicities of compounds were evaluated by SRB method.Results and Conclusion Five alkaloid compounds were isolated and identified as fructigenines A(1),deacetyl fructigenines A(2),fructigenines B(3),1,4-benzodiazepine-2,5-diones(4) and cyclopenin(5),respectively.Compound 4 and 5 showed cytotoxicity at the concentration of 3 ?g?mL~(-1)against tsFT210 cell line.

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Determination of nutrient constituents of Luidia quinaria von Martens

Guangchang PAN ; Chenghua GUO

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To analyse the nutritional compontents of Luidia quinaria Von Martens collected from the Yellow Sea,and to provide the theoretical bases for exploitation and utilization of its resource,.Methods The contents of total suger,crude fat and protein were determined by anthorone reagent,improved Soxhlet extraction and micro-Kjeldahl method,respectively.The analysis of fatty acid composition was determined by gas chromatography.Results The contents of total suger,reducing suger,crude fat and protein from Luidia quinaria Von Martens were 2.64%,1.68%,2.71% and 22.20%,respectively.Unsaturated fatty acid was rich in the Luidia quinaria Von Martens,especially EPA was 45% of the total(li-)pid,and chemical elements were rich.Conclusion Luidia quinaria Von Martens was rich in nutritional constituents and was worthy to be utilized.

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Optimization of extraction technology of the polysaccharides from sporophyll of Undaria pinnatifida Suringar by orthogonal design

Liang ZHU ; Qing ZHANG ; Qinchang ZHU ; Yifei WANG ; Yinzhou CEN

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To study the extraction technology of the polysaccharides from Sporophyll of Undaria pinnatifida Suringar and find out the optimum extraction condition.Methods The extraction technology was studied by the orthogonal design.Results The optimum extraction conditions were to add 50 times of water to the Undaria pinnatifida Suringar material at 90℃ for 4 hours.Conclusion This optimum extraction technology based on the orthogonal design was confirmed by the experiment and was available in the future research.

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Preparation of ?-carotene microemulsion from Dunaliella salina and its stability

Qiling TANG

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To preparare the ?-carotene microemulsion (?-cm)from Dunaliella salina and study its stability.Methods The influences of some factors on the stability of ?-cm were studied.These factors included light,temperature,air(oxygen),storing time and so on.Results The appearance,color,Zeta potential,volume-Wt diameter and K_(e) were unchanged,but content of ?-cm was decreased with higher temperature(40℃)or stronger light(4500?500L_(x)).Conclusion The preparation should be stored at low temperature and keep away from light.Under this circumstance,the ?-cm was stable for more than 12 months.

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Study on the HPLC fingerprints quality control of ascidian Styela plicata

Chunrong LI ; Fanlin ZENG ; Jiemiao QIU ; Xinxiang WAN

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To establish the HPLC fingerprints for the guality control of ascidian Styela plicata.Methods The HPLC fingerprints of ten batches of samples were obtained using ZORBAX Bonus-RP(4.6?250mm,5?m) column with a mix of acetonitrile and water with 0.01%TFA as mobile phase in a gradient mode,the detection wavelength was 254nm and the temperature was 30℃.Results The experiment and analysis were carried out on ten samples,the standard HPLC fingerprint pattern method and 13 characteristic diffraction peaks of Styela plicata were obtained.The proposed method was precise,reproducible and steady.Conclusion This reliable and convenient method can be used for the identification and quality control of Styela plicata.

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A novel ceramide from starfish Asterias rollestoni Bell—rollceramide A

Yongcheng ZHAN

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To study the chemical constituents of starfish Asterias rollestoni Bell.Methods The constituents were isolated by chromatography methods and identified by spectroscopic and chemical analyses.Results and Conclusion A novel compound was obtained and identified as(2S,3S,4R)-2-(13'-methyl-pentadecanoylamino)-nondecane-1,3,4-triol.The compound was reported first time and named as rollceramide A.

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Screening of cytotoxic activity of marine animal symbiotic and epiphyte microorganisms against B16 tumor cell

Xinqiang XIE

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To isolate marine microorganisms against murine melanoma cells B16.Methods Using Gause,M1,and Martin media for isolation.Isolates were fermented in the soy flour medium,the fermentation supernatant were tested against murine melanoma cells B16 by observing the growth inhibition or killing of the tumour cells under microscope.The results were statistically analyzed by SAS.Results Six hundred and thirty four strains of marine microorganisms were isolated from 101 marine animal samples,of which 56 active strains against murine melanoma cells B16 were screened out,the percentage of cytotoxic active strains was 8.83%.Conclusion The number of cytotoxic active strains was higher from Actina,Mytilus edulis,Meretrix meretrix,Atrina,Ascidiacea,Tube Worm,and crabs.There was significant relation between different microorganism types isolated to the sampling locations.Hainan Lingshui New Village fishing row had the highest percentage of the actinomycetes,GuangXi Beihai Guchengling mangrove had the highest percentage of bacteria,and GuangXi Beihai Nanwan habor had the highest percentage of fungi.There was significant relation between cytotoxic activity of the isolated strains to different microorganism types of bacteria,actinomycetes and fungi,among which thebacteria showed highest percentage of cytotoxic activity.

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Neuroprotective effects and mechanism of phycocyanin in cerebral ischemia reperfusion injury in rats

Yunliang GUO ; Xiaojie DING ; Feng SUN ; Chao WANG

Chinese Journal of Marine Drugs.2000;0(06):-.

Objective To investigate the neuroprotective effects and mechanism of phycocyanin in cerebral ischemia reperfusion injury in rats.Methods The model of middle cerebral artery occlusion reperfusion(MCAO/R) was established using the intraluminal filament occlusion with healthy adult male Wistar rats treated by phycocyanin.The apoptosis and the expression of bFGF and FGFR-1,IGF-1 and IGF-1R,iNOS and SOD were respectively determined by TUNEL and immunohistochemical staining to evaluate the effects of phycocyanin on above indexes.Results ① The rats showed neurobehavioral function disorders and the number of nerve cells reduced while apoptotic cells increased in ischemic area after ischemic reperfusion.In phycocyanin group,the number of apoptotic cells reduced siginificantly during reperfusion 12h~3d and the neurobehavioral function was better than that those in control group during reperfusion 7~14d.② In control group,the expressions of bFGF and FGFR-1 increased successfully from reperfusion 6h and reached a maximum at 1d,then subsided gradually in cortex and striatum.In phycocyanin group,the numbers of bFGF and FGFR-1 positive cells were higher than those in control group at the same time-points,which were significantly at reperfusion 1d and 12h~3d respectively.③ The expressions of IGF-1 and IGF-1R increased in cortex and striatum following cerebral ischemic and reperfusion.In phycocyanin group,the numbers of IGF-1 and IGF-1R positive cells in each time-point were higher than those in control group,which was significantly during reperfusion 6h~1d.④ In cotex and striatum,the iNOS and SOD expressed strongly and keep high level during 6h~7d with the maximum at reperfusion 1d.In phycocyanin group,iNOS expressed significantly higher during 12h~1d whlie SOD lower during 6h~1d than those in control group at the same time-point.Conclusion Phycocyanin might play a intrinsic antioxide effect by up-regulating SOD and down-regulating iNOS to inhibit neuronal apoptosis,and enhance the neuronal repairation by means of inducing the expressions of bFGF and IGF-1 following cerebral ischemic reperfusion in rats.

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