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Antioxidation and chemical characterization of essential oil from Pelargonium graveolens.L'Her

Chunkai WANG ; Weijing QU ; Wei SUN ; Yuju LI

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To study medical effect of the antioxidation activity of essential oil from smell storusbill(Pelargonium graveolens,L'Her)(PLO) METHODS: Free radicals(O?_2、?OH and DPPH?)scavenging activities were investigated in different special chemical systems.Its anti-lipoperoxidantion activity was studied with MDA generation model in vitro.The effect of the hemolysis of cavy RBC induced by H_2O_2 was also studied.The components of PLO was identified by GC-FID. RESULTS: It was found that PLO showed higher anti-lipoperoxidantion on MDA generation spontaneously in homogenates of cavy heart,liver and kidney.There were significant differences on lipid peroxidant of the cavy liver homogenate induced by CCl_4 between control group and PLO.There were higher scavenging effect of the extracts of foliage on O?_2 and DPPH?PLO could strongly inhibit the hemolysis of cavy RBC induced by H_2O_2.The result of GC-FID and DPPH? scavenging activities indicated that the antioxidantion of citronellol was similar with PLO. CONCLUSION: PLO is a good antioxidation and citronellol is the main antioxidated substance in PLO.

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Protectiv eeffect of Shexiang Baoxin Pills on the heart function and cardiac pathologic changes of STZ rats

Xi WU ; Lin ZHANG ; Linuo ZHOU ; Hong LIU ; Renming HU ; Xixing ZHU

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM:To observe the effect of Shexiang Baoxin Pills on pathologic changes and heart function of streptozotocin(STZ)-induced diabetic rats. METHODS:The rats were randomly divided into the control group,Shexiang Baoxin Pill group and STZ group.The rats in Shexiang Baoxin Pill group took Shexiang Baoxin Pill(50 mg/kg) to perfuse the stomach every day.The cardiac functions in 8 weeksafter treatment were determined.Meantime,the cardiac pathologic changes were observed through electron microscope and immunohistochemistry. RESULTS: Shexiang Baoxin Pills could reduce the interstitial fibrosis of myocardium in STZ rats.it could obviously improve the cardiac contraction and diastolic functions in STZ rats. CONCLUSION: Shexiang Baoxin Pills can(amendment) cardiac pathologic changes and improve cardiac function in STZ rats.

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Effects of Corydalis decumbens Pers Injection on Ang-1 expression of hippocampus in vascular dementia rats

Limei YU

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To observe the effects of Corydalis decumbens Pers Injection(CDPI) on the learning,memory and Ang-1 expression of hippocampus in vascular dementia rats,and to explore the protective mechanisms. METHOD: The VD model rat model was established by the ligation of both carotids of the rat.Morris water-maze was used to examine the ability of spatial learning and memory of VD model rats.Hippocampal histopathological change was observed through optical microscope after being stained with Hematoxylin-eosin(HE).The semi quantitative analysis of Ang-1 expression in hippocampus was analysed by BI2000 image analysis system after immunohistochemistry staining. RESULTS: In the place naviga test,the mean escape latency significantly shorted and the explporing distance obviously decreased in CDPI 2 mg/kg、5 mg/kg groups after one month and two months compared with model group.After two months with CDPI treatment,Ang-1 exprssion of hippocampus increased in VD rats. CONCLUSIONS: CDPI can improve the ability of spatial learning and memory.The mechanism may be related to increasing expression of Ang-1 in hippocampus.

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Effects of the total paeony glycoside on the focal cerebral ischemia in rats

Renqiang MA ; Banghao ZHU ; Jianwen CHEN ; Canhua QIU ; Peiqing LIU

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To investigate the protective effects of the total paeony glycoside(TPG) on the focal cerebral ischemia and regional cerebral blood flow(rCBF) in rats. METHOD: The rat model of focal cerebral ischemia was made by middle cerebral artery occlusion(MCAO) with nylon suture.TPG was injected into every group rats once a day before 48 h,and injected before MCAO 30 min and after 4 h,12 h.After 24 h the effects of the drug were studied about neurological deficit,the water content of brain tissue,the cerebral infarcted zone,under microscopic examination,as well as rCBF on each rat with laser Doppler fiowmeter(LDF). RESULTS: The sympton of brain ischemia was obvious in model rats by contrast to the sham rats,and the model rats rCBF decreased markedly after MCAO.50 mg/kg and 100 mg/kg TPG injection could obviously promote neurological deficit,decrease the water content of brain tissue and the cerebral infarcted zone.And the pathological slices also proved its protective effect on neuron.The laser Doppler flowmeter detected result indicated that 100 mg/kg TPG inject could greatly increase MCAO rats rCBF. CONCLUSIONS: TPG injection has a marked prospective activity on rat focal brain ischemia in rats,and the increase of rCBF may be one of the protection mechanism.

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Clinical study on therapeutic effect of Supplemented Sijunzi Decoction on chronic ulcerativ colitis

Shouya YU

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To study the clinical effect of Supplemented Sijunzi Decoction on chronic ulcerativ colitis. METHODS: 124 cases as treament group were treated with Supplemented Sijunzi Decoction,56 cases as control group were treated with azulfidine and fluperacid.A period of treatment was ten weeks. RESULTS: The total(effective) rates of clinical symptom improvement in the treament group and the control group were 90.32% and 75% respectively,and there was significant difference between two groups(P

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Determination of cinobufagin and resibufogenin in Xinkening Capsules by HPLC

Shuying LI ; Hailin DONG ; Xiao LIANG

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To establish the method of determining cinobufagin and resibufogenin in Xinkening Capsules(Venenum Bufonis,etc.). METHODS: HPLC was applied to determining cinobufagin and resibufogenin.The determination was carried out using an ODS column with a solvent system: methanol-water(50∶40).The flow rate was 1.0 mL/min,detection wavelength was at 296 nm. RESULTS: The linear ranges of cinobufagin and resibufogenin were 3.5 ?g-0.28 ?g and 3.75 ?g-0.30 ?g,respectively.The average recoveries of cinobufagin and resibufogenin were 99.86%(RSD=1.8%,n=5),and 99.84%(RSD=1.2%,n=5),respectively. CONCLUSION: This method is simple,accurate with strong specificity and can be used to control the quality of Xinkening Capsules.

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Determination of psoralen and isopsoralen in Bushen Yinao Capsules

Xuesong LI ; Xingdong FAN ; Zhubang YUAN ; Changcheng ZHUO ; Xiaolin LI

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To establish the method of determining psoralen and isopsoralen in Bushen Yinao Capsules(Fructus Psoraleae,Radix et Rhizoma Ginseng Rubra,Poria,etc.). METHODS: HPLC conditions were as(follows:) Chromasil C_(18) column,a mobile phase of CH_3OH-H_2O(55∶45),and the wavelength at 247 nm. RESULTS: This method showed a good linear relationship in the ranges of 0.038-0.309 ?g(psoralen) and 0.036-0.295 ?g(isopsoralen).The average recoveries were 99.50% for psoralen(RSD was 0.91%,n=5) and 100.52% for isopsoralen(RSD was 1.70%,n=5). CONCLUSION: The method is simple,swift,accurate and practical in quality control of Bushen Yinao Capsules.

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Determination of(+)-catechin and(-)-epicatechin and berberine hydrochloride in Wangying Capsule by RP-HPLC

Fenyun SONG ; Fuhai WU ; Zhaojian ZHONG

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To determine the content of(+)-catechin and (-)-epicatechin and berberine hydrochloride in Wangying Capsule(Rhizoma Picrorhizae,Rhizoma Coptidis,Catechu,etc.). METHODS: A RP-HPLC method was established.The chromatographic column was Diamonsil-C_(18).The mobile phase was 0.04 mol/L citric acid-N,N-dimethylformamide-tetrahydrofuran(40∶8∶2) for determining(+)-catechin and(-)-epicatechin with the flow rate of 1.0 mL/min,and the detection wavelength of 280 nm.The mobile phase was acetonitrile-water(52∶48)(including 0.34% potassium dihydrogen phosphate and 0.17% sodium dodecyl sulfate) for determining berberine hydrochloride with the flow rate of 1.0 mL/min,and the detection wavelength of 265 nm. RESULTS: The average recoveries for(+)-catechin and(-)-epicatechin and berberine hydrochloride were 100.9% and 99.9% and 97.0%,precision of the method were 2.52% and 2.53% and 2.70%(RSD,n=6),respectively. CONCLUSION: The method can be used for quantitative determination of the preparation.

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Quality standard of Haimo Blood-Stanching Tablets

Yunyun JIANG ; Guorong FAN

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To establish the quality standard for Haimo Blood-Stanching Tablets(cuttle fish sepia) by means of quantitative and qualitative experiments. METHODS: Melanin was identified by physics and chemistry reaction.Gly,Asp,Glu and Ala in Haimo Blood-Stanching Tablets were determined by HPLC-AccQ-Tag method and iron was determined by atomic absorption spectrophotometer. RESULTS: Melanin in sepia could be dissolved in 2 mol/L NaOH and there was something black separated out when in 2 mol/L HCl.The recoveries of four kinds of(amino) acids and iron were all in the range of 95%-105%.Each RSD was below 5%(n=6). CONCLUSION: The quantitative and qualitative analysis methods are accurate and reproducible.This study provides a method for the quality control of Haimo Blood-Stanching Tablets.

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Extraction and seperation of indirubin in Folium Isatidis

Guoxiang XIE ; Liguo ZHANG ; Mingfeng QIU ; Wei JIA

Chinese Traditional Patent Medicine.1992;0(06):-.

AIM: To study the suitable conditions of the extraction-separation process of the indirubin in Folium Isatidis with NIR. METHODS: The indirubin was chromatographed on an aluminium oxide column and chloroform-ethyl acetate as eluting agent,eluate was recrystalized by methanol-acetone. RESULTS: The indirubin extracted by this method was of high purity (97.78%).The sample purity was similar to reference substance one((98.01%)).The spectra of NMR and IR are the same. CONCLUSION: This method is proved to be fast,safe,lower cost,simple and has good results.

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