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Determination of icariin and adenosine in Cordyceps Vigorine Tablet by HPLC

Qiang MA ; Yuxin ZHOU ; Haimin LEI ; Changhai WANG

Chinese Traditional Patent Medicine.2005;27(6):648-650.

AIM: To establish HPLC for the determination of icariin and adenosine in Cordyceps Vigorine Tablet. METHODS:MetaChem Polaris ODS C18-A column(4.6 mm × 250 mm, 5 μm) was adopted; acetonitrile-water was the mobile phase; detection wavelength was set at 272 nm for icariin and at 260 nm for adenosine; column linearity within the range of 0. 374-2. 246 μg for icariin and 0. 348-1. 392 μg for adenosine, respectively. The average recovery and RSD were found to be (99.4 ± 1.84) % for icariin ( n = 6), (99.63 ± 1.29 ) % for adenosine,respectively. CONCLUSION: The method is convenient, fast, reliable to operate and suitable for the quality control of Cordyceps Vigorine Tablet.

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Determination of tanshinone Ⅱ A in Compound Dangguifukang Capsule by RP-HPLC

Xinchun WANG ; Zhigeng YU ; Weijun CHEN ; Zhilin HU

Chinese Traditional Patent Medicine.2005;27(4):424-426.

AIM: To establish a HPLC method for the determination of tanshinone Ⅱ A in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C18(5μm, 250mm×4.6mm), protective column: (5μm, 12.50mm×4.6mm); mobile phase: methnaol-ultrapure water (80: 20); detective wavelength was at 270a good linearity within the range of 1×10-2-0.16μg, r=0.9995. The average recovery was 99.73% and RSD was 2.91%. CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.

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Comparative analysis of ursolic acid in Hawthorn leaves by HPLC

Ronghua LIU ; Boyang YU ; Shengxiang QIU ; Dan ZHENG

Chinese Traditional Patent Medicine.2005;27(3):318-322.

AIM: To develop a simple HPLC for the determination of ursolic acid in Hawthorn leaves, and to compare ursolic acid content in Hawthorn leaves of different species, locations and growth stages, so as to supply some evidences for the exploitation and utilization of Hawthorn leaves reasonably. METHODS: By high-performance liquid chromatography method. Lichrospher C18 column (250 ×4.6 mm I. D. 5 μm); mobile phase, acetonitrile-water-orthophosphoric acid (85: 14.95: 0.05) with a flow-rate of 1.00 ml/min; column temperature at 30 ℃; injection volume, 5μl; UV detector at 210 nm. RESULTS: The detection limit (S/N=3) was less than 4. 024 μg/ml and the limit of quantification( S/N =10) was less than 12.05 μg/ml. The calibration curve showed good linear regression(r =0. 9999) within measurement ranges( 16.09 - 1030 μg/ml). The intra-day and interday variation were 0.71% and 6. 15%, respectively. The recoveries at low to high concentration were 89%-105%. Under these conditions, the ursolic acid content in different Hawthorn leaves were determined: 1.90%-1.95% in C. scabrifolia (Franch.) Rehd, 1.00%-1.45% in C. cuneata Sieb. & Zucc, 0.45%-0.65% in C.pinnatifida Bge. var. major N. E. Br.; In differnet growth stages of C. pinnatifida Bge. var. major N. E. Br. , the young leaves contain higher content of ursolic acid. CONCLUSION: The method is successfully applied to quantify ursolic acid in Hawthorn leaves. And the ursolic acid contents in Hawthorn leaves of differnent species are very different; C. scabrifolia (Franch.) Rehd contains the highest ursolic acid content in them. However, there is a little difference among different locations and growth stages for same species.

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Accelerated Test on Stability of Chaitong Tablets (Radix Bupleuri)

Qinglong DING ; Lianming XU

Chinese Traditional Patent Medicine.1992;0(03):-.

Study on stability of Caitong Tablets with the constant temperature accelerated test has been carried out. Using the flavone component in Caitong Tablets as an index,their content was determined. It was predetermined that the shelf life was 4. 11 years.

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Comparative study on crystal structures and contents of inorganic elements in prevention solution of mica before and after being calcined

Xia DING

Chinese Traditional Patent Medicine.1992;0(03):-.

AIM: To explore the machanism of calcined mica by comparing the difference in crystal structure and inorganic elements of raw mica and calcined ones. METHODS: Crystal structures were compared by IR spectrum and X-ray crystallography and the contents of element in the prevention were determined with ICP-AES. RESULTS: After being calcined,crystal phase transformation changed in gold mica and did not change in white mica.Both of their chemical composition changed,crystal water decreased and crystal defects increased.There were obvious differences in the contents of inorganic elements.The content in white mica was obviously more than that in gold mica.After being calcined,the value of K/Na in white mica decreased and the contents of Ca,Mg increased,while the content of Mg increased and the content of Ca decreased in gold mica. CONCLUSION: There are(actually) many differences in contents of elements and chemical structure in white and gold mica before and after being calcined,which may explain the mechanism of calcined mica.

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Determination simultaneously of gentiopicroside and geniposide in Longdan Xiegan Pill by HPLC

Ruixin LIU

Chinese Traditional Patent Medicine.1992;0(08):-.

AIM:To establish a HPLC method for determining simultaneously gentiopicroside and geniposide in Longdan Xiegan Pill(Radix et rhizoma Gentianae,Fructus Gardeniae,Radix Scutellariae,etc.).METHODS:C_ 18 column(5 ?m,4.6 mm?250 mm)was used.The mobile phase was methanol-water(23∶77).The detecting wavelength and the temperature of column were at 238 nm and at 40 ℃ respectively.RESULTS:The linear detection range of gentiopicroside was 3.03-30.30 ?g/mL(r=1.000 0),and the average recovery was 98.43%(RSD=1.08%).The linear detection range of geniposide was 9.76-97.60 ?g/mL(r=0.999 9),and the average recovery was 99.95%(RSD=0.91%).CONCLUSION:Two ingredients in Longdan Xiegan Pill could be determined simultaneously by this method which is convenient,rapid and accurate.It can be used for quality control in the production of Longdan Xiegan Pill.

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Effect of Shujinhuoxuedingtong Wine on Microcircuiation of Experimental Mice

Ruixing WEN ; Xiangdong ZHOU

Chinese Traditional Patent Medicine.1992;0(01):-.

The method for examining the microcirculation bloodflow of mice mesentery was used to evaluate the action of Shujinhuoxuedingtong wine against microcirculation disturbance caused by adrenaline. The result indicates that the wine can obviously improve the microcirculation function and is responsible for the antistatic action observed. The wine's effect is found to be superior to that of Shujinhuoxuedingtong Powder. The above fingings suggest that the wine have the action of promoting blood circulation to remove blood stasis and improving microcirculation and the effect of promoting the bloodflow perfusion quantity in tissue organs. It's effect is prior to that of powder.

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Content De termination of Ephedrine Hydrochloride in Xiaoqingiong Oral Liquid

Laige TONG ; Guigen YAO

Chinese Traditional Patent Medicine.1992;0(01):-.

Isolate the ephedrine from other compositions in Xiaoqinglong Oral Liquid with the steam distillation utilizing its volatility* then determine the ephedrine hydro-chloride content in this preparation with bromothymol blue colorimetry.The methods are accurate and their results are satisfactory. It is suggested that they could be used in quality control for the preparation.

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Content Determination of Icariin in Fuchun Tablets with Reversed-phase High-efficiency Liquid Chromatography

Jian NI ; Yuan ZHOU

Chinese Traditional Patent Medicine.1992;0(01):-.

A simple, rapid and accurate method is described for the determination of icariin in Fuchun Tablets by reversed-phase high-efficiency liquid chromatography. The samples were first extracted with 70% alcohol for 50 minutes The resulting solutions were chromatographed on aYWG-C18 column.By using a mobile phase of methanoltetrahydrofuran-water (26.5:15.9:56.7) and a detection wavelength at 274 nm, the recovery for 101.02%, CV=2.4%,are obtained.The method can be employed for the quality control of Fuchun Tablets.

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Pharmaceutical Study on Bushen Oral Liquid

Zhirong WU ; Jiansheng TAO

Chinese Traditional Patent Medicine.1992;0(02):-.

The content determination of betaine in Lycium Chinese Mill with TLC-scanner have been carried out.The betaine content can be regarded as an index for studying the preparation process and quality control of Bushen Oral Liquid.

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