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Determination of icariin and adenosine in Cordyceps Vigorine Tablet by HPLC

Qiang MA ; Yuxin ZHOU ; Haimin LEI ; Changhai WANG

Chinese Traditional Patent Medicine.2005;27(6):648-650.

AIM: To establish HPLC for the determination of icariin and adenosine in Cordyceps Vigorine Tablet. METHODS:MetaChem Polaris ODS C18-A column(4.6 mm × 250 mm, 5 μm) was adopted; acetonitrile-water was the mobile phase; detection wavelength was set at 272 nm for icariin and at 260 nm for adenosine; column linearity within the range of 0. 374-2. 246 μg for icariin and 0. 348-1. 392 μg for adenosine, respectively. The average recovery and RSD were found to be (99.4 ± 1.84) % for icariin ( n = 6), (99.63 ± 1.29 ) % for adenosine,respectively. CONCLUSION: The method is convenient, fast, reliable to operate and suitable for the quality control of Cordyceps Vigorine Tablet.

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Determination of tanshinone Ⅱ A in Compound Dangguifukang Capsule by RP-HPLC

Xinchun WANG ; Zhigeng YU ; Weijun CHEN ; Zhilin HU

Chinese Traditional Patent Medicine.2005;27(4):424-426.

AIM: To establish a HPLC method for the determination of tanshinone Ⅱ A in Compound Danguifukang Capsule. METHODS: Analytic column: Reliasil C18(5μm, 250mm×4.6mm), protective column: (5μm, 12.50mm×4.6mm); mobile phase: methnaol-ultrapure water (80: 20); detective wavelength was at 270a good linearity within the range of 1×10-2-0.16μg, r=0.9995. The average recovery was 99.73% and RSD was 2.91%. CONCLUSION: The method is feasible, and simple to operate and suitable for the quality control of Compound Dangguifukang Capsule.

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Comparative analysis of ursolic acid in Hawthorn leaves by HPLC

Ronghua LIU ; Boyang YU ; Shengxiang QIU ; Dan ZHENG

Chinese Traditional Patent Medicine.2005;27(3):318-322.

AIM: To develop a simple HPLC for the determination of ursolic acid in Hawthorn leaves, and to compare ursolic acid content in Hawthorn leaves of different species, locations and growth stages, so as to supply some evidences for the exploitation and utilization of Hawthorn leaves reasonably. METHODS: By high-performance liquid chromatography method. Lichrospher C18 column (250 ×4.6 mm I. D. 5 μm); mobile phase, acetonitrile-water-orthophosphoric acid (85: 14.95: 0.05) with a flow-rate of 1.00 ml/min; column temperature at 30 ℃; injection volume, 5μl; UV detector at 210 nm. RESULTS: The detection limit (S/N=3) was less than 4. 024 μg/ml and the limit of quantification( S/N =10) was less than 12.05 μg/ml. The calibration curve showed good linear regression(r =0. 9999) within measurement ranges( 16.09 - 1030 μg/ml). The intra-day and interday variation were 0.71% and 6. 15%, respectively. The recoveries at low to high concentration were 89%-105%. Under these conditions, the ursolic acid content in different Hawthorn leaves were determined: 1.90%-1.95% in C. scabrifolia (Franch.) Rehd, 1.00%-1.45% in C. cuneata Sieb. & Zucc, 0.45%-0.65% in C.pinnatifida Bge. var. major N. E. Br.; In differnet growth stages of C. pinnatifida Bge. var. major N. E. Br. , the young leaves contain higher content of ursolic acid. CONCLUSION: The method is successfully applied to quantify ursolic acid in Hawthorn leaves. And the ursolic acid contents in Hawthorn leaves of differnent species are very different; C. scabrifolia (Franch.) Rehd contains the highest ursolic acid content in them. However, there is a little difference among different locations and growth stages for same species.

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Dynamic changes of five constituents in Ligustri lucidi Fructus at five picking time

Fucheng WANG ; Donghan JI ; Chengwu FANG ; Daofeng GONG ; Chengkai XIA

Chinese Traditional Patent Medicine.2017;39(5):1011-1014. doi:10.3969/j.issn.1001-1528.2017.05.027

AIM To analyze the dynamic changes of five constituents in Ligustri lucidi Fructus at five picking time (August,September,October,November,December).METHODS The HPLC analysis of Ligustri lucidi Fructus ethanol extract was performed on a 25 ℃ thermostatic Aglient Zorbax SB-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 224 nm.RESULTS Salidroside,tyrosol,luteolin-7-O-glucoside,ligustroflavone and specnuezhenide showed good linear relationships within their own ranges (r ＞0.999 0),whose average recoveries were 99.56％-100.30％ with the RSDs of 0.89％-1.23％.The contents of various constituents (except for tyrosol) were the highest in samples picked up in September,followed by those picked up in October.CONCLUSION The suitable picking time of Ligustri lucidi Fructus is September and October.

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Determination of two constituents in rat liver microsomal incubation system and their metabolism

Shaodong DENG ; Ying WANG ; Wenchang ZHAO ; Fengxia XIAO ; Jingran LIN ; Qianqian DUAN

Chinese Traditional Patent Medicine.2017;39(5):1002-1005. doi:10.3969/j.issn.1001-1528.2017.05.025

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Determination of three constituents in Polygonum aviculare by UPLC

Junli YANG ; Yongsheng BAN ; Yazhong ZHANG ; Lidan HUANG

Chinese Traditional Patent Medicine.2017;39(5):998-1002. doi:10.3969/j.issn.1001-1528.2017.05.024

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Determination of four constituents in the productive process of Gardeniae Fructus intermediates in Reduning Injection

Xiaoqian XU ; Yang MA ; Wenzhe HUANG ; Yuan BI ; Zhenzhong WANG ; Wei XIAO ; Gang DING

Chinese Traditional Patent Medicine.2017;39(5):993-997. doi:10.3969/j.issn.1001-1528.2017.05.023

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Determination of chemical consituents in Lycii Fructus from Qaidam Basin

Youfeng WU ; Shizhen MA ; Liang TAN ; Haisheng FENG ; Caixia LI

Chinese Traditional Patent Medicine.2017;39(5):984-989. doi:10.3969/j.issn.1001-1528.2017.05.021

AIM To determine the contents of chemical consituents in Lycii Fructus from Qaidam Basin.METHODS Spectrophotometry was adopted in the content determination of polysaccharides,total flavonoids and carotenoid.HPLC was applied to the content determination of betaine and scopoletin.Kjeldahl method was used for the content determination of protein.Then principal component analysis was performed.RESULTS The contents of carotenoid,betaine and scopoletin in samples from six growing areas showed obvious differences (P ＜ 0.05),while those of polysaccharides,total flavonoids and protein exhibited no obvious differences (P ＞ 0.05).The contents of various constituents in samples at three picking time also had no obvious differences (P ＞ 0.05).The comprehensive score of principal components of samples from Delingha City was the highest,followed by that from Ulan County.CONCLUSION The quality of Lycii Fructus from Qaidam Basin from Delingha City is the best.

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Analysis of constituents migrating to blood in Suoquan Pills after stir-frying with salt-water of Alpinia oxyphylla

Mengqi LI ; Xiaomeng GONG ; Shanshan WU ; Lin HU ; Changjiang HU

Chinese Traditional Patent Medicine.2017;39(8):1675-1678. doi:10.3969/j.issn.1001-1528.2017.08.028

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Determination of three oligosaccharide esters in Polygalae Radix and its three processed products by HPLC

Ruqiao LUAN ; Xuelan ZHANG ; Peng WU ; Huaixing KANG ; Huifen LI ; Jiawei DIAO ; Baoxin XU ; Xin ZHAO

Chinese Traditional Patent Medicine.2017;39(8):1666-1669. doi:10.3969/j.issn.1001-1528.2017.08.026

AIM To determine the contents of arillanin A,tenuifoliside A and tenuifoliside C in raw Polygalae Radix (root barks),Polygalae Radix duramen,Glycyrrhizae Radix et Rhizoma-processed Polygalae Radix,waterboiling Polygalae Radix and honey-processed Polygalae Radix.METHODS The analyses of 50％ methanol extracts from samples were performed on a 30 ℃ thermostatic Kromasil C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 330 nm.RESULTS The contents of three oligosaccharide esters were the highest in raw Polygalae Radix,followed by those in honey-processed Polygalae Radix,and those in water-boiling Polygalae Radix were the lowest.These constituents also existed in Polygalae Radix duramen,but their contents were lower than those in root barks.CONCLUSION The ester bonds of oligosaccharide esters in Polygalae Radix may be hydrolyzed during processing,followed by the generation of small molecular organic acids.The medication of whole Polygalae Radix (root barks and duramen) can be taken into consideration in clinical practice to reduce toxicity and enhance efficacy.

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