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Clinical Experience of WANG Yan-gang in Using Huazhuo Jiedu Method for Treating Stomach Cold

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):111-113. doi:10.3969/j.issn.1005-5304.2017.07.027

This article briefly discussed the academic thoughts and clinical experience of Professor WANG Yan-gang in treating the stomach cold from the theory of Zhuodu. According to the theory of Zhuodu, Professor WANG Yan-gang usesHuazhuo Jiedumethod to relieve or significantly ease the symptoms of stomach cold by exploring the reasons through syndrome differentiation and treatment based on reason.

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Review on Experience of CUI Shu-sheng's Practice in Treating Insomnia

Zongxian YU ; Honglei DING

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):108-110. doi:10.3969/j.issn.1005-5304.2017.07.026

Insomnia is the important factor affecting people's lives. The long-term lacking of sleep can not only affects people's daily life, but also can lead to a variety of mental illness. With the development of society, the life and working styles of people have changed a lot, and the number of patients with insomnia is increasing. However, the main therapy of insomnia is the Western medicine with calming and sedative efficacy. This kind of medicine has side effects, and long-term application can cause resistance and addiction. This article introduced the fourth batch of 100 old TCM doctors Professor CUI Shu-sheng's profound understanding of insomnia etiology and pathogenesis. Combined with the function features of Tuina, he summarized the Ten Methods of head massage. The Ten Methods of head massage has the characteristics which are light but not float, heavy but not hurt, and gentle and strong. The main operation places are head and face; thus, it can relieve the scalp and dredge qi and blood of head and face. Because the doctor can guide patients to calm down and focus on their public region and soothe their body, it has the effects of harmonizing yin and yang, calming, and soothing.

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Experience of Professor LI Ying-cun in Using Dunhuang Zisu Decoction for Treating Pediatric Internal Injury Cough

Zheng GE ; Xinhao LI ; Xiaoyi YANG ; Lijuan LIANG

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):105-108. doi:10.3969/j.issn.1005-5304.2017.07.025

Professor LI Ying-cun believes that pediatric internal injury cough is due to the weakness of lung and spleen and endogenous phlegm. The lung loses dispersing and descending to retrograde and cause cough; spleen deficiency produces phlegm to exist in the lung. The children have delicate organs need strengthening the body resistance, dispersing lung and invigorating spleen, supplementing qi and consolidating the exterior.ZisuDecoction is one of the TCM prescriptions that found from Dunhuang ancient medical prescriptions. Professor LI Ying-cun flexiblely uses this ancient prescription in modern clinic, which has achieved good efficacy in treatment of pediatric internal injury cough.

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Discussion on DU Ke-kuan's Academic Thoughts and Clinical Experience in Treating Children with Spleen and Stomach Diseases

Yishi TU

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):102-104. doi:10.3969/j.issn.1005-5304.2017.07.024

Professor DU Ke-kuan pays attention to the special role of the spleen and stomach in pediatric diseases, good at grasping the relationship between physiological and pathological features of spleen and stomach in childre with relative viscera. He also thinks that eliminating pathogenic factors is very important, which should be combined with supporting healthy, inhibiting the liver to strengthen the spleen, understanding the pathogenesis and combining internal and external treatment for the spleen-stomach diseases in children, with a strong guidance and practicality.

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Discussion on Medication Rules of Cinnamomi Ramulus inShang Han Lun from Dose-Response Relationship

Wei ZHANG

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):98-101. doi:10.3969/j.issn.1005-5304.2017.07.023

Objective To provide references for clinical medication through the analysis on the dose-response relationship and compatibility medication rules of Cinnamomi Ramulus.Methods It systematically arranged the provisions containing Cinnamomi Ramulus inShang Han Lun. Relationship between the dose of Cinnamomi Ramulus and the related factors were analyzed by single factor logistic regression analysis and binary correlation analysis by SPSS20.0 statistical software.Results After screening, 40 prescriptions including Cinnamomi Ramulus were selected, accounting for 35.40% in the total prescriptions ofShang Han Lun. The taken dose of every time of Cinnamomi Ramulus had a close relationship with the number of medicine and water consumption, with statistical significance (P=0.000,P=0.004). However, it had no relationship with residual water, water consumption per time and use frequency, without statistical significance (P>0.05). Single factor logistic regression analysis showed whether Cinnamomi Ramulus as a mainmedicine had the close relationship with the taken dose of every time of Cinnamomi Ramulus and use frequency (P=0.008,P=0.043). When Cinnamomi Ramulus and Glycyrrhizae Radix et Rhizoma Praeparata cum Mella were in compatibility, compared with when Cinnamomi Ramulus was not the main medicine, the taken dose of every time of Cinnamomi Ramulus as the main medicine in the group was more frequently used, with statistical significance (P=0.046); however, the number of medicine was statistically lower than when Cinnamomi Ramulus was not main medicine (P=0.043). When Cinnamomi Ramulus and Ephedrae Herba were in compatibility, there was no statistical difference in the taken dose of every time of Cinnamomi Ramulus or the number of medicine (P>0.05). When Cinnamomi Ramulus was the main medicine, it was not in compatibility with Poria or Bupleuri Radix.ConclusionFrom the dose-response relationship, it can be found that the use and compatibility of Cinnamomi Ramulus inShang Han Lun are strict and flexible, and dose law can provide a basis for clinical application.

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Comparison Study on Fingerprint Chromatograms of Dipsaci Radix Decoction Pieces, Aqueous Decoction and Formula Granules by HPLC

Le GAO ; Shuaishuai FAN ; Xinguo WANG ; Wei TIAN ; Zhong CHEN ; Liying NIU

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):81-85. doi:10.3969/j.issn.1005-5304.2017.07.019

Objective To establish and compare HPLC fingerprint chromatograms of Dipsaci Radix decoction pieces, aqueous decoction and formula granules.Methods The HPLC analysis was carried out in Wondasil C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase of acetonitrile-0.1% phosphoric acid by gradient elution. The flow rate was 1.0 mL/min; the detection wavelength was set at 212 nm; the column temperature was kept at 30℃. Results The fingerprint chromatograms from 12 batches of Dipsaci Radix decoction pieces, aqueous decoction and formula granules were established respectively. 14 common peaks in the fingerprint chromatogram in the formula granules could be tracked in the aqueous decoction, and 13 common peaks in the fingerprint chromatogram could be tracked in the decoction pieces. 2 chemical compounds were identified, such as asperosaponinⅥ and chlorogenic acid.ConclusionThe method of HPLC fingerprint chromatograms is stable and with good repeatability. Dipsaci Radix decoction pieces, aqueous decoction and formula granules are basically the same chemical composition.

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Optimization of Ethanol Extraction Technology ofLichong ShengsuiDecoction by Multi-index Orthogonal Test

Yanhong WANG ; Juanping ZHAO ; Dongxue SHANG ; Xue ZHAO ; Miao TIAN ; Fengjuan HAN

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):76-80. doi:10.3969/j.issn.1005-5304.2017.07.018

Objective To optimize the ethanol extraction technology ofLichong ShengsuiDecoction.Methods An L9(34) orthogonal test was used in the study. The extraction rates of calycosin glycoside, icariin, baohuosideⅠ, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, astragaloside, and ginsenoside Rd were set as indexes. The influence of ethanol concentration, extraction time, extraction temperature, and amount of ethanol on the yield of Lichong ShengsuiDecoction were detected by comprehensive scoring method.Results The optimal ethanol extraction technology forLichong ShengsuiDecoction was soaking for 2 h with ten times of 60% ethanol and then reflux extracting for two times; extraction time was 1.5 h each time at 80℃.ConclusionThe optimal extraction technology is efficient, stable and feasible, which can provides data for the further study ofLichong ShengsuiDecoction.

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Optimization of Compound Enzyme Extraction Technology of Total Flavonoids from Lycii Fructus by Response Surface Method

Jiande GAO ; Xiaoyu ZHU ; Xiong LIU ; Hui CHEN ; Kairong SONG ; Tingting SHI

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):66-71. doi:10.3969/j.issn.1005-5304.2017.07.016

Objective To optimize the extraction technology of total flavonoids from Lycii Fructus by response surface methodology (RSM).Methods On the basis of single-factor tests, enzyme concentration, enzymolysis time, and enzyme solution temperature were selected as influencing factors during extraction to conduct three-factor three-level center combination design. The effects of three factors on the yield of total flavonoids from Lycii Fructus were analyzed by RSM.Results The optimum conditions for extraction technology of total flavonoids from Lycii Fructus were enzyme concentration 0.30%, enzymolysis time 1.0 h, enzyme solution temperature 60℃. The predicted extraction yield of total flavonoids was 0.9492%.ConclusionUsing RSM to optimize the extraction technology of total flavonoids from Lycii Fructus is stable, feasible, and simple, which can provide references for further study and application.

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Effects of Modified Danshen Decoction on SSAT Activity of Rats with Myocardial Ischemia/Reperfusion Injury

Ting REN ; Chunmei RAO ; Xihua CHENG ; Shenghui YANG ; Yanbo SUN ; Cong CHEN ; Xia PENG ; Zhengde HUANG

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):62-65. doi:10.3969/j.issn.1005-5304.2017.07.015

Objective To observe the effects of modifiedDanshen Decoction on spermidine/spermine acetyltransferase (SSAT) /polyamine pathways of SD rats with IRI; To investigate its protective mechanism. Methods The model of IRI was established by ligating left anterior descending coronary artery for 30 min followed by reperfusion for 90 min. The SD rats were randomly divided into the control group, sham-operation group, model group and modifiedDanshen Decoction group, with 10 rats in each group. The myocardial infarction size was measured by using TTC staining. The contents of SSAT were measured by ELISA. The SSAT mRNA and SSAT protein expression level were detected with real-time fluorescent quantitative PCR method and Western blot, respectively. The contents of polyamines (putrescine, spermidine, spermine) in cardiac tissue were detected by HPLC. Results Compared with sham-operation group, the myocardial infarction size, the SSAT content, the SSAT mRNA and SSAT protein expression levels of model group increased significantly, the contents of polyamines decreased significantly, with statistical significance (P<0.01); Compared with model group, the myocardial infarction size of modifiedDanshen Decoction group was significantly reduced, while the SSAT content and SSAT mRNA and protein expression level decreased significantly, the contents of polyamines increased, with statistical significance (P<0.05, P<0.01).ConclusionModifiedDanshen Decoction can adjust the SSAT polyamine pathways and increase polyamine content in cardiomyocytes, and thus play a role of protection of myocardial ischemia-reperfusion injury.

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Applicability of Zebra Fish Thrombosis Model in Antithrombotic Activity Screening of Chinese Materia Medica

Jiaojiao FAN ; Yihan QIAO ; Chongjun ZHAO ; Yuanyuan NI ; Ran YANG ; Yaru FENG ; Zhiqiang MA ; Ruichao LIN

Chinese Journal of Information on Traditional Chinese Medicine.2017;24(7):58-61. doi:10.3969/j.issn.1005-5304.2017.07.014

Objective To investigate the applicability of zebra fish thrombosis model in antithrombotic activity screening of Chinese materia medica.Methods The living zebra fish thrombosis model was induced by adrenaline hydrochloride. Zebra fish were randomly divided into blank control group, model group, positive medicine group and medication group. Each group was given the corresponding medicine or embryo culture water. O-anisidine staining solution was used to stain and calculate the staining intensity of erythrocytes in zebra fish heart, and quantitative analysis was carried out. The platelet aggregation of transgenic zebra fish was observed and under qualitative analysis. Results Compared with the model group, 100μg/mL salvianolic acid B, 300, 900μg/mL aqueous extract of Salvia miltiorrhiza, 45μg/mL 95% ethanol extract and 400, 1200μg/mL hypothalamus could significantly inhibited the formation of zebra fish thrombosis (P<0.01).ConclusionZebra fish thrombosis model has good applicability in antithrombotic activity screening of Chinese materia medica.

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