Chinese Journal of Information on Traditional Chinese Medicine 2017;24(7):66-71

doi:10.3969/j.issn.1005-5304.2017.07.016

Optimization of Compound Enzyme Extraction Technology of Total Flavonoids from Lycii Fructus by Response Surface Method

Jiande GAO ; Xiaoyu ZHU ; Xiong LIU ; Hui CHEN ; Kairong SONG ; Tingting SHI

Keywords

response surface method; technology optimization; compound enzyme extraction; total flavonoids from Lycii Fructus

Country

China

Language

Chinese

Abstract

Objective To optimize the extraction technology of total flavonoids from Lycii Fructus by response surface methodology (RSM).Methods On the basis of single-factor tests, enzyme concentration, enzymolysis time, and enzyme solution temperature were selected as influencing factors during extraction to conduct three-factor three-level center combination design. The effects of three factors on the yield of total flavonoids from Lycii Fructus were analyzed by RSM.Results The optimum conditions for extraction technology of total flavonoids from Lycii Fructus were enzyme concentration 0.30%, enzymolysis time 1.0 h, enzyme solution temperature 60℃. The predicted extraction yield of total flavonoids was 0.9492%.ConclusionUsing RSM to optimize the extraction technology of total flavonoids from Lycii Fructus is stable, feasible, and simple, which can provide references for further study and application.