Objective: To eplore the effective measure to prevent postopelative deep vein thrombosis (DVT).Methods:510 cases of hospitalzed surgical patients from January 2005 to December 2007 were divied into observation group(without any preventive measure) and control group(including physiotheragy group,elastic socks with gradient pressure group and intermittent pressure device group).To observe the patients changes in 10 days after operation.Results:There were 6 cases of DVT in 125 cases of observation group(with the occurrence rate of 4.8%).In control group,there was I case of DVT in 125 cases of physiotherapy group(with the occurrence rate of 0.8%) and no cases of DVT in the other two observation groups.Conclusion:The elastic soks with gradient pressure and the intermittent pressure device can effectively prevent the occurrence of postoperative DVT.

Objective: To discuss the effects of nursing intervention in the rebleed rate of the cirrhosis patients after endoscopic esophageal varix ligation.Methods:60 cirrhosis patients with endoscopic esophageal varix ligation were randomly divided into intervention(n=30)and control group(n=30).Both groups received usual care and the intervention group did additional nursing intervention as psychological nursing health education relaxation training,effective coping strategy,familial and social support system.Resets:The rebleed rate was significantly lower.the time of hospital stay shorter and the hospital expense less in the intervention than in the control group(P<0.05). Conclusion:The nursing intervention in the patients with endoscopic esophageal varix ligation can obviously decrease the rebleed rate, abate reduce their economic burden and improve their quality of life.

Objective: To explore the clinical value of color Doppler ultrasound in evaluation of carotid artery before and after treatment of diabetes cases.Methods:A total of 80 cases of Type 2 Diabetes were examined with color Doppler ultrasound before and after treatment in Carotidor. Their carotid artery wall,lumen,intima-media thickness and plaque size,and flow conditions were analyzed respectively, ResuLts: In 80 cases of type 2 diabetes detection rates of carotid artery atherosclerotic plaque after treatment were lower than those before treatment,and the carotid artery diameter,Intima-media thickness and resistance index were reduced too. Conclusion:Using color Doppler ultrasound in carotid artery,can be directly observe large vascular wall and luminal lesions,and has a important clinical value in the prevention of diabetic carotid atherosclerosis and formulation the means of intervention,and it has broad application prospects in suppression of diabetic macrovascular disease development.

Objective: To investigate the relationship between glycated albumin levels and diabetic nephropathy in patients with type 2 diabetes mellitus.Methods: From January 2006 to June 2009,78 patients with type 2 diabetes mellitus in our hospital were enrolled in the study. We used liquid enzymatic to detect glycated albumin,and compare with 50 cases of healthy in the same period as the control group.Results: The glycated albumin was different between the control group and the diabetic nephropathy groups(F=8.953,P=0.000). Glycated albumin in each diabetes group was higher than that in the control group;glycated albumin in diabetic nephropathy groups was higher than that in non-diabetic nephropathy group;glycated albumin in end-stage diabetic nephropathy group was higher than that in the rest of groups.The severity of diabetic nephropathy and level of glycated albumin were related,rs was 0.754(t=6.547,P=0.008).Logistic regression showed that glycated albumin was an independent risk factor impacting on the incidence of diabetic nephropathy and the severity of the disease,multi-adjusted OR value was 2.326,and 95%CI was 2.114-2.538.Conclusions:Glyeated albumin plays an important role in the pathogenesis of diabetic nephropathy ,and it is closely related to the phases of diabetic nephropathy.

Objective: To discuss the effect of bone marrow mobilization on chemotaxis of aggregation of macrophages in venous thrombosis. Methods:SD adult rats were divided randomly into normal group,sham operation group,thrombus group and rhG-CSF group.Peripheral blood was harvested at 30min before operation and on the 7th and 14th day after operation.Level of MCP-3 in peripheral blood was detected by ELISA.Inferior vena cava specimens of thromus group and rhG-CSF group were collected at postoperative 7th and 14th day. With the application of RT-PCR,expression of MCP-3mRNA in thrombus was detected.Results:The level of MCP-3 in peripheral blood of rhG-CSF group was significantly higher than that before operation and also higher than the levels of MCP-3 in peripheral blood of sham operation group and thrombus group on the 14th day after operation(P<0.05),and the difference was significant.On the 7th and 14th day after operation,compared with thrombus group,the expression of MCP-3 mRNA in rhG-CSF group remarkablely increased,and the difference Was significant beween these two groups(P<0.01).Conclusion:Brone marrow cell mobilization enhances the chemotaxis of MCP-3 on macrophages.

Objective: The aim of this study was to investigate the immune function and prognosis for patients with primary hepatic cancer who implemented the perioperative enteral immunonutrition support for hepatectomy.Methods: 58 cases of patients with primary hepatic cancer in our hospital in 2003 treated with liver resection were randomly divided into nutritional support fur immune nutrition group and conventional control group.Immune function was detected on admission,1st day and 7th after surgery;5-year follow-up was performed to compare the prognosis of the two groups. Results: Concentration level of CD3+,CD4+,CD8+and CD4+ICD8+in preoperative hepatectomy were the same between the two groups. On the first day after surgery,CD3+,CD4+,CD8+content decreased significantly; there was no difference between the two groups. On the 7th day after surgery,CD3+,CD4+,CD8+content increased;and the intervention group increased more significantly.1,2,3,4,and 5-year survival rates in intervention group and control group were 82.8%,70.2%, 39.6%,22.6%.20.6%and 74.6%,50.1%,25.4%,21.5%,19.5%.The survival rate was higher in the intervention group than the control group at 1-year,2-year and 3-year,while the 4 and 5-yearsurvival rate of the two groups was same,and the intervention group had better overall survival compared with the control group(X~2=3.547,P=0.025).The OR value for enteral immunonutrition support impacting postoperative survival was 0.649 after multivariate analysis adjusted.with 95.0%CI of 0.614-0.686.Conclusion:Enteral immunonutrition support can ameliorate immune suppression in hepatectomy for patients with primary liver cancer during perioperative period,and it also Can improve postoperative survival.

By means of crossed immuno-affinoelectrophresis,serum fucosylated AFP(Fuc AFP)was determined in 167 cases, including primary hepatic carcinoma (PHC) (72 cases), various liver diseases(54 cases), other gastrointestinal tumors (4 cases),and healthy controls (37 cases) .The results showed that serum fucosylation index of AFP (FucI) was significantly higher in PHC than in controls and other diseases (P

The authers employed combinations of sequential (NH4)2 SO4 precipitation, DE-52 ion exchange chromatography,Sephadex G-200 gel filtration to isolate SP1 from retroplacental blood collected during labour. Anti-SP1 affinity chromatography has proven helpful in purification. The final product was shown about 207 fold of purification. Purified SP1 reacts with anti-SP1 serum from Behringwerke AG, migrates as p globulin in immunoelectrophoresis. SDS polyacrylamide gradient gel electroph-oresis indicates a major band with molecular weight around 90000. The antiserum obtained by immunization of rabbits with purified SP1 after appropriate absorption shows a single peak in cross immunoelectrophoresis, fails to react with serum from normal nonpregnant females and males, yields single line showing identity with SP1 antiserum from Behringwerke AG in immunodiffusion with pregnant plasma. This antiserum has been successfully used in establishing ELISA for SP1

By using the method of eross-sectional anatomy, we examined human eerebral asymmetries in 31 Chinese adult brain.The left cortical language areas is larger than the right. The distribution of the hemispheric asymmetries is compatible with that reported by CT scan.The asymmetry of the posterior language areas mainly reflect the asymmetry of the occipital length and the asymmetries of the wide occipital lobe and the wide occipital horn might be in relation to the handedness. Though both the anterior and the posterior language area are mostly larger on the left hemisphere,there is no relationship between them

The GABA hypothesis of hepatic encephalopathy (H.E) was supported by many reports with regard to its content, metabolism, permeability against blood brain barrier, etc. But the experimental data which reflect the changes of GABA recep-ptors in H.E. were limitted only to rabbits and rats.We established a method with a small concentration of membrane protein (50ug/ 0.2ml) for the analysis of GABA receptors with satisfactory results (NSB, TB, SB: CV