Journal Detail Information

1

Cite

Share

THE EFFECTS OF LENTINAN ON THE PROLIFERATIVE RESPONSE OF MOUSE SPLEEN LYMPHOCYTES IN VITRO

Chinese Pharmacological Bulletin.1986;0(05):-.

Lentinan could enhance the proliferative response of the mouse spleen lymphocytes to FHA, LPS ( Lipopolysaccharides ) and MLR ( Mixed Lymphocyte Reaction ) in vitro in a concentration-dependent manner. At the concentration of 25M-g/ml, it had the most prominent effects and the enhancing rates were 42.1%, 24.1% and 27.1% respectively. At much higher or lower concentrations, however, it had almost no effects. But lentinan at 25H-g/ml could inhibit the prolifera-tire response to Con A ( Concanavalin A ) and the inhibitory action increased with the increase in the concentration. At the concentration of 200 p-g/ml, the inhibition rate was 52.2%.

2

Cite

Share

INHIBITION OF MITOGEN-INDUCED LYMPHOCYTE PROLI-FERATION BY BIMOLANE

Ning DAN ; Tanmu ZHANG

Chinese Pharmacological Bulletin.1986;0(05):-.

It has been reported that bimolane can inhibit either humoral or cell-mediated immunological responses in vivo in mice & the inhibition of humoral immunity (by bimolane) is stronger than that of cellular immunity. Author investigated the influence of bimolane on the mitogen-induced mouse lymphocyte proliferation. Bimolane inhibited the lymphocyte proliferation induced by Con A(concana-valin A), PHA ( phy tohemagglutinin ) or LPS(lipopoiysaccharide) . Bimolane dose-dependently inhibited LPS-induced lymphocyte proliferation with remarkable inhibition at 1.56 mg/L bimolane. The inhibition of Con A-induced or PHA-induced lymphocyte proliferation by bimolane, however, began to appear at above 6.25 mg/L bimolane. The bimolane-mediated inhibition of the proliferation of the lymphocytes was not due to a nonspecific toxicity, because the preincubation of spleen cells with 25 mg/L bimolane for 24h did not make the cells died tested by trypanblau exclusion method.The data suggest that B lymphocytes are more sensitive to the bimolane-mediated inhibition than T lymphocytes.

3

Cite

Share

STUDY OF THE FRACTION XVII-B_2 FROM THE VENOM OF NAJA NAJA ATRA

Ze ZHANG ; Benyong HU

Chinese Pharmacological Bulletin.1986;0(05):-.

The venom of Naja naja atra from Guangdong province has been separated into 27 fractions by CM-Sephadex C-25 column chromatog-raphy. One lethal toxin fraction was further purified by ion-exchange rechromatography on CM-Sephadex C-50 and by gel filtration on Sep-hadex G-50 and was named fraction XVII-B2. It can abolish the responses of chikea biventer cervisis muscle to indirect stimulation or to Ach. but not affects the responses to direct stimulation Or K+. On the other hand, it has no effects on Straub's frog hearts in vitro,but its LD50 for mice is about 75ug/kg.Slab-PAGE, SDS-PAGE and isoelectrofocusing were performed to test the homogeneity of the fraction. Some immunological methods were also used. The molecular weight and pl for this fraction are about 7200 daltons by SDS-PAGE and pH 8.5 by isoelectrofocusing. The resemblance between the fraction and cobratoxin has been analysed by comparing the amino acid composition. We have used it successfully in radio ligand assay of nicotinic receptor preparations from Narcine maculata electric organ.The experiment data show that the fraction may be a short post-synaptic neurotoxin and may be an effective tool In studies of acetyl-choline receptor.

4

Cite

Share

EFFECTS OF POLYACTIN A ON THE HEMOLYSIN ANTIBODY PRODUCTION IN MICE

Tianran WANG ; Zhixun XIE

Chinese Pharmacological Bulletin.1987;0(03):-.

In this report, the immunopharmacological effects of Polyactin A ( PAA ) were presented. When the SRBC immunized mice were administered daily 2. 5~40mg/kg ( ip ) of PAA for 3 or 5 days, the serum level of hemolysin, the antibody production in spleen and the number of nucleated cells of spleen were enhanced significantly; and the efficacy-dose dependency was evident in these experiments。 In spite of these effects, PAA didn't affect the kinetics of circulating antibody level.In other experiments, it was found that the efficiency was more potent when PAA was given before or simultaneously with SRBC immunization than given after.

5

Cite

Share

Design and discovery of novel small-molecule inhibitor of CDK9

Li QIN ; Qing JI ; Yingdai GAO ; Juanni LIU ; Ming YANG ; Xiaofeng SHAO ; Dongsheng XIONG

Chinese Pharmacological Bulletin.2003;0(10):-.

Aim To look for novel small-molecule inhibitors of CDK9 through structure-based virtual screening and biological activity determination.Methods Homology modeling of CDK9 was based on the 3-D structure of other cyclin-dependent kinase family members,and then virtual screening by DOCK(molecular docking)of database of small molecule was carried on.MTT method was used in inhibition of tumor cell growth in vitro,while Western blot was used for further study of molecular mechanisms.Results From the top 1000 compounds with the best DOCK energy score,27 compounds were selected for biological assay based on the diversity of chemical structure and functional group.12 of 27 selected compounds showed significantly inhibition activity on tumor cell proliferation,and only one compound in 12 with half-maximum inhibition concentration(IC50)values less than 20 ?mol?L-1 named C-21 was selected for further molecular mechanism study.The western blotting data showed C-21 compound could effectively inhibit CDK9 from phosphorylating large subunit C-terminal of RNA polymerase Ⅱ in a dose-dependent manner.Conclusions Through homology modeling,virtual screening by computer,determination of biological activity and experimental studies of molecular mechanism,a new promising lead compound targeted for CDK9 was found and confirmed.

6

Cite

Share

Relationship between amnestic effect of enflurane or isoflurane and NMDA receptor

Dan WANG ; Tijun DAI ; Tao MA ; Jing MENG

Chinese Pharmacological Bulletin.2003;0(10):-.

Aim To investigate the relationship between amnestic effect of enflurane or isoflurane and NMDA receptor.Methods Amnestic model was established by intraperitoneal injection of enflurane(0.4 ml?kg-1)or isoflurane(0.3 ml?kg-1)respectively in mice before intracerebroventricular injection of different doses of NMDA(25,50,75 ng),then the error times,step down latency and step through latency were observed in the step down test and step through test.Results NMDA(50,75 ng)by intracerebroventricular injection could decrease the error times,and increase the step down latency and step through latency of amnestic mice induced by enflurane or isoflurane in the step down test and step through test.Conclusions NMDA by intracerebroventricular injection can improve amnestic effect of enflurane or isoflurane partially.NMDA receptor may be an important target for amnestic effect of enflurane or isoflurane.

7

Cite

Share

Current research progress in glutamate transporter subtype GLT-1 and its regulated drugs

Chengmin LI ; Hui YAN ; Zehui GONG

Chinese Pharmacological Bulletin.2003;0(10):-.

The homeostasis of extracellular glutamate concentration is critically regulated by glutamate transporters(GTs).Malfunction or decreased expression of GTs has been implicated in the pathogenesis of various nervous system diseases.And among the GTs,glutamate transporter 1(GLT-1)plays a critical role as "glutamate pump".Recent research also finds some GLT-1 variants which alternate C-terminal splicings.Altered expression of proteins encoded by splice variants of GLT-1 has been noted in a number of disease states.Maintaining a physiological range of extracellular glutamate through regulating GTs expression or function may improve some pathological conditions.Many drugs are reported that can regulate GTs.For example,ceftriaxone,phencyclidine,citicoline,riluzole,AKT,thrombosin can up-regulate the expression or function of glutamate transporters;etomidate,clozapine,aspartic acid analogs,endothelin can down-regulate the expression or function of glutamate transporters.In this paper,the drugs which effect GTs will be summarized in order to provide a new insight into the drug design and clinical treatment of neurological diseases.

8

Cite

Share

Effects of prostaglandin on osteoporosis in ovariectomized rats

Yuyu LIU ; Liao CUI ; Weimin YAO ; Bilian XU ; Tie WU

Chinese Pharmacological Bulletin.2003;0(10):-.

Aim To investigate the preventive effects of misoprostol on osteoporosis in aged ovariectomized(OVX)rats.Methods Female,10-month-old SD rats were ovariectomized(OVX)and,2 months later,were treated with misoprostol or controls for 2 months.The static and dynamic parameters in trabecular bone of the forth lumbar vertebrae(LV4)were examined with histomorphometrical analyses;the fifth lumbar vertebrae(LV5)was used to perform the compression test.Results Compared with the data from the sham-operated rats,the percent trabecular area and elastic modulus significantly decreased in OVX rats.Correspondingly,the bone break load and break stress decreased of post OVX was compared with those of sham-operated rats.Misoprostol increased the percent trabecular area by 21.6% compared with OVX rats,but it couldn't meet the statistical significance.Misoprostol enhanced the break load and elastic modulus compared with OVX rats.Conclusion Misoprostol can improve biomechanics of bone in ovariectomized osteoporosis rats.

9

Cite

Share

The apoptosis-inducing effect of MnSOD_m on K562 cells and its molecular mechanism

Linlan FAN ; Hulai WEI ; Wei DOU ; Weisheng LIU ; Huifang ZHANG

Chinese Pharmacological Bulletin.2003;0(10):-.

Aim To explore the apoptotic effect of mimics of manganese superoxide dismutase(MnSODm)on human leukemia cell line K562 in vitro and the possible molecular mechanisms.Methods Human leukemia K562 cells were used as the target cells.The cell proliferating activity was examined by a MTT colorimetric assay,and the apoptosis of K562 cells was assessed with FITC-Annexin V and propidium iodide(PI)double staining and morphological changes.The expressions of bcl-2 and bax mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR),and flow cytometry(FCM)was employed to measure the expressions of Bcl-2 and Bax protein,mitochondrial inner membrane potential(??m),Cytochrome C(Cyt C)release and Caspase-3 activity.Results The proliferation of K562 cells was obviously inhibited by 0.5~10 mg?L-1 MnSODm(P

10

Cite

Share

The expression of TRAIL in psoriatic patients and healthy subjects

Jishun YANG ; Jianhua WU ; Quangang ZHU ; Jiyong LIU ; Jinhong HU

Chinese Pharmacological Bulletin.2003;0(10):-.

Aim To explore the expression of TNF-related apoptosis-inducing ligand(TRAIL)between psoriatic patients and healthy subjects.Methods Immunohistochemical staining and Real-Time PCR methods were established to detect the expression of TRAIL.Results Immunohistochemical staining results indicated a significant difference of the expression of TRAIL between psoriatic patients and healthy subjects.Real-Time PCR results indicated that the quantities were 0.42?0.07,1.01?0.16 respectively of two type skins.Thus there were significant differences(P

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share