Journal Detail Information

1

Cite

Share

An acute lung injury model caused by seawater aspiration in rats

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To establish an acute lung injury model by seawater aspiration in rats.Methods: Twenty-four rats were randomly allocated into 2 groups: control group(n=9) and model group(n=15).The acute lung injury model was established in rats by aspirating 2 ml seawater/kg body weight after 9-10 seconds of breath occlusion.The aspiration was repeated by the same means 2 min later.Rats in control group aspirated no seawater.Arterial blood gases and respiratory rates were observed before aspiration and 10 min,30 min,1 h,2 h and 4 h after aspiration.The survival times of animal were also recorded.The rats were sacrificed 4 h after aspiration;the lung index,total protein(TP) contents,white blood cell(WBC) count in bronchoalveolar lavage fluid(BALF),and the pulmonary histopathology were observed.Results: Compared with control group,model group had a significantly increased respiratory rate and a decreased PaO_(2)(P

2

Cite

Share

Role of bone marrow cells in renewal of renal tubular epithelial cells under physiological condition

Baochun LI

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To observe whether bone marrow cells (BMCs) can differentiate into the renal tubular epithelial cells under physiological condition.Methods: Transgenic C57BL/6 mice labeled with green flouresent protein(GFP) served as bone marrow donors and C57BL/6 mice without fluorescence labeling were divided into 3 groups: normal group(N,n=15),total body irradiation group(TBI,n=15),and bone marrow transplantation(BMT,n=15) group.The mice in Group N received no treatment,those in Group TBI were irradiated with lethal dose of ? rays but received no transplant(sacrificed within 1 week after irradiation),and those in Group BMT were transplanted with BMCs after the irradiation(sacrificed at 58 and 84 days after transplantation).Blood samples were obtained from all 3 groups for routine analysis and analysis of urea nitrogen and plasma creatinine.Meanwhile,mice kidneys were harvested for H-E staining.The amount of GFP positive BMCs in mice of BMT group was determined by flow cytometry;the distribution and amount of GFP positive cells in renal tissues were observed by immunohistochemistry.Results: There was no difference in the levels of urea nitrogen and plasma creatinine between the 3 groups.Lethal-dose total body irradiation resulted in no significant change in renal histological structure and function,but RBC,WBC and PLT of the mice in TBI group were markedly reduced compared with those in the other 2 groups(P0.05).Conclusion: Bone marrow cells may participate in renewal of tubular epithelial cells even without the renal tubular damage.

3

Cite

Share

Development and validation of a finite element model for lumbar motion segment (L_4-L_5)

Yaosheng LIU

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To develop and validate an accurate three-dimensional geometrical and mechanical finite element(FE)model of the lumbar L_(4)-L_(5) segment using a new computer-aided designing(CAD) method.Methods: First,a modified "non-seed region segmentation" was done to extract the interest region in the CT image and to obtain a binary image,from which the iso-surface of vertebral body was produced by a discretized marching cubes algorithm.Second,"best cross-section planes" representing the morphologic characteristics of physiological lordosis were used for the initial iso-surface model,forming a "non-regular piecewise subspace".This subspace and the embedded iso-surface model were subsequently transformed by local affine transforms to a "regular subspace",in which a surface mesh of high quality was generated quickly.Finally,a reverse transform procedure was employed to restore the original three-dimensional(3D) image of the lumbar surface mesh of lumbar L_(4)-L_(5).All coordinate dada of nodal points and message of triangular patches of the surface model were then subjected to ANSYS for the three-dimensional FE mesh construction.An accurate 3D non-linear FE model of lumbar motion segment(L_(4)-L_(5)) was developed and validated against published data.Results: The constructed FE model of lumbar L_(4)-L_(5) consisted of 94 794 solid elements,1 196 link elements,1 170 shell elements,768 target elements and 464 contact elements,and included geometrical,material and contact non-linearities.The predicted results of lumbar L_(4)-L_(5) segment were closely correlated with published results of experimental biomechanics in terms of axial displacement,segment rotation and intradiscal pressure under similar load condition.Conclusion: Based on advanced algorithm,this constructed surface model of L_(4)-L_(5) segment is capable to perform whole digitalized binary image extraction and reconstruction of the lumbar surface with excellent simulation results.

4

Cite

Share

Reactive oxygen species in regulation of hypoxia-inducible factor

Xuejun SUN ; Zhaoyun PENG ; Xiaoying CHEN ; Hengyi TAO

Academic Journal of Second Military Medical University.1985;0(06):-.

Oxygen is a mandatory for all aerobic organisms. Oxygen-containing free radicals are produced when oxygen is not completely reduced to water in energy-producing oxidation reaction.The radicals may also transform into other reactive compounds through electron transfer and all the compounds with similar functions are referred as reactive oxygen species(ROS).Increased ROS is known to cause damage to proteins,DNA and lipids.Much evidence showed that changes in partial oxygen pressure,hormone,cytokine and chemical stimulation could increase ROS,and ROS,acting as signaling molecules,mediates cell functions.Hypoxia-inducing factor(HIF),a key transcriptional factor for most hypoxia-inducible genes,is a heterodimer consisting of 2 subunits.Recent study found that ROS plays an important role in HIF activity regulation under hypoxic and non-hypoxic conditions.This paper reviews the production of ROS and its role in the regulation of HIF activity.

5

Cite

Share

Mitochondrial nitric oxide synthase: biological function

Wenwu LIU ; Xuejun SUN

Academic Journal of Second Military Medical University.1985;0(06):-.

Increasing evidences have shown the existance of a mitochondrial nitric oxide synthase(mtNOS),which binds to the matrix face of the mitochondrial inner membrane and produces nitric oxide(NO) through a Ca~(2+)sensitive pathway.Under physiological condition,the NO catalyzed by mtNOS regulates mitochondrial oxygen consumption and transmembrane potential via reversible competition with cytochrome C oxidase.The reaction of NO with superoxide anion,which was produced by mitochondrial respiratory chain,yields peroxynitrite.Peroxynitrite irreversibly modifies susceptible targets in mitochondria and induces oxidative and/or nitrative stress.In addition,NO has also been implicated in the programmed cell death.This article reviews the current understanding of mtNOS's role in the regulation of mitochondrial functions.

6

Cite

Share

Real-time three dimensional echocardiography in evaluation of left ventricular volume and ejection fraction in patients with aneurysm combined with myocardial infarction

Yong XU ; Guang ZHI ; Tingshu YANG ; Luyue GE ; Qi SUN ; Xiaoxia WU

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To assess the feasibility and accuracy of real-time three dimensional echocardiography(RT-3DE) in quantitative evaluation of left ventricular(LV) volume and ejection fraction(EF) in patients with ventricular aneurysm and myocardial infarction.Methods: Twenty-three patients with left ventricular aneurysm combined with myocardial infarction were examined by RT-3DE,two dimensional echocardiography Simpson's method,and M-mode Teichholz method separately.The following parameters: left ventricular end diastolic volume(LVEDV),end systolic volume(ESV),stroke volume(SV) and EF were obtained by each method and the results were compared with those obtained by left ventriculography(LVG).Results: The values of LVEDV,LVESV,SV,and EF determined by RT-3DE showed good correlations with those determined by LVG(r=0.92,0.90,0.88,and 0.91,respectively;P(0.05).) The values of LVEDV,LVESV,SV,and EF determined by Simpson's method also showed good correlations with those determined by LVG(r=0.85,0.87,0.86,0.91,respectively;P

7

Cite

Share

Three strategies for hemostasis at femoral artery access site: a comparison of safety and efficacy

Hairong XU

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To assess the safety and efficacy of the standard manual compression and 2 arterial puncture closing devices,Angioseal and Perclose,for hemostasis at the femoral artery access site in patients undergoing coronary angiography(CAG) or percutaneous coronary interventions(PCI).Methods: Totally 366 patients undergoing coronary angiography or PCI were assigned to receive either Angioseal(n=128),Perclose(n=110) or standard manual compression(n=128).The efficacy endpoint(immediate hemostasis,successful hemostasis rate,operating time,time of leg immobilization and time to hemostasis) and safety endpoint(vasovagal reflex,major complications,local complications,hematocrit drop,etc.) were evaluated.Risk factors(gender,age,body mass index,comorbid conditions,antiplatelet agents,and anticoagulant agents,etc.) were also analyzed.Results: The successful hemostasis rates were similar between Angioseal group and Perclose group,but the rate of immediate hemostasis of Angioseal group was higher than that of Perclose group(P

8

Cite

Share

Effect of percutaneous coronary intervention on mitral regurgitation in patients with acute myocardial infarction: a 6 months follow-up

Yong XU ; Qi SUN ; Guang ZHI ; Baoshi HAN ; Luyue GE ; Tingshu YANG

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To evaluate the effect of percutaneous coronary intervention(PCI) on mitral regurgitation(MR) in patients with acute myocardial infarction(AMI).Methods: A total of 213 AMI patients were divided into PCI group(n=87,(PCI +) medication) and medication group(n=126,medication) according to the treatments they received.Echocardiographic examination was conducted in patients during admission and 6 months follow-up.Color Doppler was used to determine the degree of MR.Echocardiogram indices included MR degree,left ventricular end-diastolic diameter(LVEDd),left ventricular end-systolic diameter(LVEDs),left ventricular end-diastolic volume(LVEDV),left ventricular end-systolic volume(LVESV),and left ventricular ejection fraction(LVEF).Results: The overall incidence of MR was 28.6% in 213 patients during admission.The MR incidence in patients with acute inferior myocardial infarction was higher than that in patients with other parts of infarction((34.5%) vs 22.3%,P0.05),while the incidence in medication group increased significantly than that during admission(43.7% vs 30.2%,P

9

Cite

Share

Consistency between histopathological results of routine endoscopy and biopsy in observing gastric mucosa of patients with non-malignant gastroduodenal diseases

Haiyun YANG ; Zhizheng GE ; Shengliang CHEN ; Suying CHEN

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To analyze the consistency between the histopathological results of routine endoscopy and biopsy in observing the gastric mucosa of patients with non-malignant gastroduodenal diseases,and to evaluate the necessity of biopsy following gastric endoscopy.Methods: From Jan.2005 to Dec.2005,320 patients who received upper gastrointestinal endoscopy and biopsy because of upper abdominal symptoms were included in this study.The patients were selected consecutively according to their disorders diagnosed by macroscopic endoscopy and were divided into 64 groups.The 5 patients in each group had reflux esophagitis,non-atrophic gastritis,atrophic gastritis,gastric ulcer and duodenal ulcer,respectively.Patients in the same group were matched with each other in gender,age and their history of diseases.The results of endoscopy,including the exudation,congestion,erosion,roughness,bile reflux,etc.,were read by 2 experienced endoscopists.The biopsy was performed by an experienced pathologist and pathological variables included active inflammation,chronic inflammation,atrophy,intestinal metaplasia and atypical hyperplasia.The status of Helicobacter pylori(H.pylori) infection was evaluated by rapid urea test,silver staining and histological methods;the result was deemed positive when the results of either 2 tests were positive.Results: Erosion,exudation,roughness,and H.pylori infection were related with active inflammation;erosion and H.pylori infection were related with chronic inflammation;roughness of mucosa was related with atrophy;roughness and H.pylori infection were related with intestinal metaplasia;and obsolete hemorrhage,H.pylori infection,roughness mucosa,and bile reflux were related with atypical hyperplasia.Macroscopic diagnosis rate of atrophic gastritis was 71.9%(46/64) with a false positive rate of 28.2%(18/64) and a false negative rate of 34.38%(22/64).Conclusion: Macroscopic diagnosis is indicative to pathological changes of gastric mucosa,but the predictive value is relatively poor,making biopsy and pathological examination necessary in the diagnosis of gastric mucosa disorders during routine endoscopic examination.

10

Cite

Share

Effect of replicating adenovirus harboring p53 gene on chemosensitivity of hepatocarcinoma cell line

Jicheng DUAN

Academic Journal of Second Military Medical University.1985;0(06):-.

Objective:To construct a replicating adenovirus vector CNHK600-p53 carrying p53 gene and investigate its effect on the chemosensitivity of hepatocarcinoma cell line.Methods: Chemotherapy of hepatocarcinoma cells BEL-7404 were carried out using Fluorouracil,Mitomycin,Epirubicin,CNHK600-p53,CNHK600-p53 + Fluorouracil,CNHK600-p53 + Mitomycin,and CNHK600-p53 + Epirubicin,separately.MTT assay was used to evaluate the killing effects after therapy.Results: The inhibition rate on BEL-7404 cells was(47?3)% when using 100 ?g/ml 5-Fu,was(20?4)% when using 1 ?g/ml MMC,and was(73?2)% when using 2.5 ?g/ml EPI.The inhibition rates of BEL-740 cells in CNHK600-p53(MOI=10) + Fluorouracil(100 ?g/ml),CNHK600-p53(MOI=10) + Mitomycin(1 ?g/ml),and CNHK600-p53(MOI=10)+ Epirubicin(2.5 ?g/ml) groups were(59?4)%,(44?4)% and(86?2)%,respectively(P

DISPLAY OPTIONS

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share

Cite

Share