Objective:To construct a replicating adenovirus vector CNHK600-p53 carrying p53 gene and investigate its effect on the chemosensitivity of hepatocarcinoma cell line.Methods: Chemotherapy of hepatocarcinoma cells BEL-7404 were carried out using Fluorouracil,Mitomycin,Epirubicin,CNHK600-p53,CNHK600-p53 + Fluorouracil,CNHK600-p53 + Mitomycin,and CNHK600-p53 + Epirubicin,separately.MTT assay was used to evaluate the killing effects after therapy.Results: The inhibition rate on BEL-7404 cells was(47?3)% when using 100 ?g/ml 5-Fu,was(20?4)% when using 1 ?g/ml MMC,and was(73?2)% when using 2.5 ?g/ml EPI.The inhibition rates of BEL-740 cells in CNHK600-p53(MOI=10) + Fluorouracil(100 ?g/ml),CNHK600-p53(MOI=10) + Mitomycin(1 ?g/ml),and CNHK600-p53(MOI=10)+ Epirubicin(2.5 ?g/ml) groups were(59?4)%,(44?4)% and(86?2)%,respectively(P