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Establishment of a Research and Assessment System Using High Quality Non-Human Primates.

Byung Hwa HYUN ; Byeong Cheol KANG ; Chuel Kyu KIM ; Heui Soo KIM ; Jun Gyo SUH ; Fumiaki CHO

Laboratory Animal Research.2010;26(4):449-457. doi:10.5625/lar.2010.26.4.449

At present, eight non-human primate research facilities exist in Korea to examine the validity and safety of new bio-products, and to generate model animal systems using primates of low health status (low quality primates). However specific-pathogen free (SPF) primates (high quality primates) are the preferred choice for emerging disease studies and for numerous other research areas, including cell/gene therapy, stem cell research, regenerative studies, and brain science. Although international primate centers in developed countries have utilized high quality primate resources for many years, there has been little or no collaboration with less developed countries on primate research. Due to this, the establishment of a high quality primate research capacity is a core priority for the advancement of the biomedical research field in less developed countries. In this study, we investigated the demand for, and opportunities to support the development of this research capability.
Animals ; Brain ; Cooperative Behavior ; Developed Countries ; Developing Countries ; Korea ; Primates ; Stem Cell Research

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Comparison on the Embryological Terms between the Korean Veterinary Anatomical Terminology (2008) and the Korean Anatomical Terminology (1996)/the Korean Medical Association: Medical Terminology (2009).

Dae Joong KIM

Laboratory Animal Research.2010;26(4):439-447. doi:10.5625/lar.2010.26.4.439

Since the first edition of the Korean Veterinary Anatomical Terminology (KVAT) in 1993, the new edition of KVAT has been revised and harmonized with the recent version of the Korean-English Anatomical Terminology (KAT, 1996 and 2005). The Korean Association of Veterinary Anatomists (KAVA) had been decided the new revised edition based on the Japanese-English Veterinary Anatomical Terminology (JVAT, third edition, 2000) at the first. The references had been changed from JVAT (2000) into the Nomina Anatomica Veterinaria (NAV, fifth edition, 2005) and the Nomina Embryologica Veterinaria (NEV, revised second edition, 2006) except the Nomina Histologica (NH, revised 2nd edition, 1994). Terminologia Histologica (TH), international terms for human cytology and histology, had been published by the Federative International Committee on Anatomical Terminology (FICAT) and approved by the International Federation of Associations of Anatomists (IFAA) at 2008. The histological-related terms have to changed by the harmonization and the unification. It has more perfect Korean spelling standardization system compared with the Korean embryological terms of the fourth edition of the Korean-English Anatomical Terminology (1996) because of lacking the recent revised references in the embryological terms in humans. The fifth edition of the Korean Medical Association: Medical Terminology (KMA-MT) has been published at 2009. The Korean version of the NEV terms has some inadequate terms that should be corrected with the harmonization with KMA-MT (2009). I hope that the harmonization and standardization of the English/Korean of the veterinary anatomical terminology will be useful in the future evolution of terminology.
Anatomists ; Humans

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The Need for the Introduction of Assessment Program of Good Animal Care and Use Facility.

Sang Koo LEE ; Shin Woo CHA

Laboratory Animal Research.2010;26(4):433-438. doi:10.5625/lar.2010.26.4.433

The humane care and use of animals should be the prime concern and responsibility of all those individuals using animals for research, teaching or testing. The use of animals as experimental subjects in the 20th century has contributed to many important advances in scientific and medical knowledge. Although scientists have also developed non-animal models for research, these models often cannot completely mimic the complex human or animal body. Nevertheless, efforts to develop and use scientifically valid alternatives, adjuncts, and refinements to animal research should continue. Laboratory Animal Law that was prepared to improve the reliability and reproducibility of research data using laboratory animals also required an institutes to care and use animal in ways judged to be scientifically, technically, and humanely appropriate. The law introduced Assessment Program of Good Animal Care and Use Facility. The primary goal of the Assessment Program is to provide quality assurance for the care and use of experimental animals on a national basis. Although, an effective surveillance protects animals, promotes animal welfare and ensures the quality of science that uses animals, it is the responsibility of investigators to conduct animal experiment on the basis of the highest scientific, humane, and ethical principles. It is expected that the Program will accelerate the introduction of mind concerning humane and ethical animal experiment and will enhance animal well-being, the quality of biomedical research, and the advancement of biologic knowledge that is relevant to humans or animals.
Academies and Institutes ; Animal Experimentation ; Animal Welfare ; Animals ; Animals, Laboratory ; Humans ; Hydrazines ; Jurisprudence ; Research Personnel

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Rectal Prolapse Associated with Recurrent Diarrhea in a Laboratory Cynomolgus Monkey (Macaca fascicularis).

Sang Rae LEE ; Yong Hoon LEE ; Kyoung Min KIM ; Sung Woo KIM ; Kang Jin JUNG ; Young Hyun KIM ; Hwa Young SON ; Kyu Tae CHANG

Laboratory Animal Research.2010;26(4):429-432. doi:10.5625/lar.2010.26.4.429

Rectal prolapse is a protrusion of one or more layers of the rectum through the anus. A 5-year-old laboratory cynomolgus monkey who had suffered from recurrent diarrhea died after surgical resection of a prolapsed rectum. On examination, the prolapsed rectum was a cylinder-shaped tissue whose surface was moist and dark red with a small amount of hemorrhage. Histologically, the rectum was characterized by a segmental to diffuse cellular infiltration in the submucosa and muscle layers. Inflammation in the rectum resulted in irritation of the myenteric plexus, which could cause hypermotility of the intestines, leading to chronic diarrhea. Rectal prolapse would result in economical loss or death of laboratory animals. However, rectal prolapse in the laboratory monkey could be easily overlooked because diarrhea or other symptoms resulting from rectal prolapse could be sometimes misunderstood as a primary problem. Therefore, researchers should suspect rectal prolapse if intestinal symptoms in the laboratory monkey are untreatable.
Anal Canal ; Animals, Laboratory ; Diarrhea ; Haplorhini ; Hemorrhage ; Inflammation ; Intestines ; Macaca fascicularis ; Muscles ; Myenteric Plexus ; Preschool Child ; Rectal Prolapse ; Rectum

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Laboratory Rodents Negatively Affected by Construction Environment.

Byeong Han LEE ; Hyun Don JUNG ; Byung Noh LEE ; Kyu Sang PARK ; Jin Soo HAN

Laboratory Animal Research.2010;26(4):425-428. doi:10.5625/lar.2010.26.4.425

This report describes rodents in a laboratory animal facility that was adversely affected by a noisy environment during construction work. There was much noise and vibration as well as dust caused by the drilling and hammering. The noise levels, frequencies, and length of time when occurring in the drilling and hammering, were all measured. The drilling showed noise levels ranging from 50-90 decibels (dB) (A-filter, A), and the hammering presented 60-70 dB (A). Some researchers raised problems regarding animal experiments, including skin injuries resulted from self-mutilation, and increase of mortality. This gives useful information to people who plan to renovate laboratory animal facilities as it is a very rare case.
Animal Experimentation ; Animal Welfare ; Animals ; Animals, Laboratory ; Dust ; Mandrillus ; Mice ; Noise ; Rats ; Rodentia ; Skin ; Vibration

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Establishment of Leukemia Mouse Model Using Mouse-Derived A20 Leukemic Cells, and Detection of Tumor Cells in Bone Marrow.

Lan Ying WEN ; Su Mi BAE ; Woong Shick AHN

Laboratory Animal Research.2010;26(4):415-423. doi:10.5625/lar.2010.26.4.415

Objectives of this study were to establish a leukemia mouse model in the Balb/c mouse based upon the A20 cell line (murine B-lymphoma/leukemia cell line, H-2d). Here we demonstrate for the first time that A20 cells were infiltrated into tissue and bone marrow, thereby evaluate the feasibility of using A20 leukemic cells as a leukemia model. In the study, changes of behavior, survival rate and histological changes of major organs after intravenous injection of A20 cells (1x105, 1x106 or 1x107) into Balb/c mice were observed. After inoculation of 1x106 cells, animals survived up to 38.3 days, although there were no significant correlation between the number of injected cells and life-span. At 21 and 28 days post-injection, both hematoxylin-eosin and CD45R immunohistochemical stains showed diffuse large B-cell lymphoma in the liver. FACS analysis was performed after injection of fluorescent nanomaterial (MNPs@SiO2 RITC)-labeled A20 cells. The labeled A20 cells were detected in bone marrow from 6 hours post-inoculation, indicative of the cellular infiltration. This is the first study that demonstrated the invasion of A20 cells into the bone marrow of Balb/c model using A20 cells. With the occurrence of systemic lesions following metastasis of the cells into lymph nodes and neighboring tissues via bone marrow infiltration, it is suggested that the A20 cell-inoculated Balb/c miouse could be an animal model of acute lymphocytic leukemia.
Animals ; Bone Marrow ; Cell Line ; Coloring Agents ; Injections, Intravenous ; Leukemia ; Liver ; Lymph Nodes ; Lymphoma, B-Cell ; Mice ; Models, Animal ; Nanostructures ; Neoplasm Metastasis ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; Survival Rate

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Antioxidative and Antidiarrheal Effects of Persimmon Extracts.

Dongsun PARK ; Sun Hee LEE ; Dae Kwon BAE ; Young Jin CHO ; Tea Kyun KIM ; Yun Hui YANG ; Goeun YANG ; Sang Chul KWON ; Do Ik LEE ; Sung Soo JOO ; Yun Bae KIM

Laboratory Animal Research.2010;26(4):407-413. doi:10.5625/lar.2010.26.4.407

Since oxidative stresses are involved in gastroenteritis and diarrhea, we investigated antioxidative and antidiarrheal activities of persimmon flesh extract (PFE) and persimmon calyx extract (PCE) in vitro and in vivo, respectively. PCE significantly scavenged 1,1-diphenyl-2-picrylhydrazyl hydrate and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) from 500 microg/mL, although PFE was ineffective. In addition, PFE and PCE exhibited strong nitric oxide-scavenging effects from 1 microg/mL, in which PCE was superior to ascorbic acid (50 microM). Furthermore, PFE and PCE significantly inhibited FeCl3-induced lipid peroxidation as well as Cu2+/H2O2-induced protein oxidation from 10 microg/mL. In vivo charcoal-propulsion assay, in contrast to a negligible effect of PFE, treatment with PCE (160-500 mg/kg) markedly inhibited intestinal motility. The results indicate that extracts of persimmon, especially PCE, possess antioxidative, antiinflammatory and antidiarrheal activities. Therefore, it is suggested that persimmon extracts could be used for the relief of gastroenteritis and diarrhea.
Ascorbic Acid ; Biphenyl Compounds ; Diarrhea ; Diospyros ; Gastroenteritis ; Gastrointestinal Motility ; Lipid Peroxidation ; Oxidative Stress ; Picrates

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Pharmacokinetic and Toxicokinetic Studies of Potential Antifungal Compounds, KAF-200522 and KAF-200522 . HCl, in Animal Models.

Kwang Han KONG ; Ju Young JUNG ; Kyo Hwan KOO ; Si Whan SONG ; Kap Ho KIM ; Zhong Ze HAN ; Seon Hwa LEE ; Ho Chul SHIN

Laboratory Animal Research.2010;26(4):399-405. doi:10.5625/lar.2010.26.4.399

Recent researches on clinically used triazole antifungal reagents are focused on their pharmacokinetic disadvantage which increases the probability of inducing adverse effects in patients. For this point, in the present laboratory, Chemon Inc., has investigated new antifungal reactive compounds, KAF-200522 and its chloride form, KAF-200522 . HCl, which has a modified triazole structure. Pharmacokinetic data were measured with LC-MS/MS in male mice which were orally treated with the above compounds at 10 mg/kg. Tmax and t1/2 of KAF-200522 . HCl were comparable to KAF-200522, but AUC and Cmax were 1.4 and 1.6 times higher than those of KAF-200522, respectively. In beagle dogs, AUC and Cmax of KAF-200522 . HCl were 2.7 and 1.4 times higher than those of KAF-200522, and t1/2 was 3.5 times higher than that of KAF-200522. Moreover, in beagle dogs, the oral bioavailability value of KAF-200522 . HCl was revealed as 31.0% to contrast to 6.2% of KAF-200522. In 1-week repeated oral treatment toxicity study of KAF-200522 in male rats, inhibition of body weight gain was observed in 120 mg/kg treatment group, and loss of body weight was observed in 600 mg/kg treatment group. In the toxicokinetic study of KAF-200522, no accumulation after the systemic exposure was observed. In conclusion, as to the new antifungal drug development, KAF-200522 . HCl was considered to be advantageous in pharmacokinetic characteristics compared to KAF-200522.
Animals ; Area Under Curve ; Biological Availability ; Body Weight ; Dogs ; Humans ; Indicators and Reagents ; Male ; Mice ; Models, Animal ; Rats

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An Improved Method for Intrathecal Catheterization in the Rat.

Young Chan JEONG ; Young Bae KWON

Laboratory Animal Research.2010;26(4):393-397. doi:10.5625/lar.2010.26.4.393

Catheterization of the subarachnoid space provides a useful tool to deliver drugs to spinal cord in rat. However, the passage of a long intrathecal catheter inserted via the cisterna magnum to lumbar spinal levels increases the risk of spinal cord trauma and the mislocation of catether into other places. Thus, present study was aimed to introduce direct subarachnoid catheterization of the rat lumbar spinal cord with novel shape of catheter. An intrathecal PE-10 catheter was fabricated with coil (four-time helix with 2 mm diameter) on either end and attached small silicon beads on both end of the coil. Using the silicon bead at insertion part as an anchorage, the catheter was advanced into the subarachnoid space until the silicon bead was embedded on a drilled hole over interspinous space between L4 and L5 vertebra. And then, silicon bead was tightly attached by surgical glue and dental resin with vertebra. Using the other side of silicon bead, catheter was tightly suturing with muscle layer. Finally, external portion of the catheter was placed into stainless steel protector on neck to reduce the damage by rat. Following this catheterization, it is not significantly affect the general physiology (i.e. motor function, weight gain and mechanical allodynia) of the rat without catheter loss over 2 weeks. Moreover, intrathecal injection either fluorescent dye or lidocaine further confirmed that drug diffusion site was appropriated for intended target.
Adhesives ; Animals ; Catheterization ; Catheters ; Diffusion ; Injections, Spinal ; Lidocaine ; Mandrillus ; Muscles ; Neck ; Rats ; Resins, Synthetic ; Silicones ; Spinal Cord ; Spinal Cord Injuries ; Spine ; Stainless Steel ; Subarachnoid Space ; Weight Gain

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Biological Activity and Single Oral Dose Toxicity of Dansam-samultang.

In Chul LEE ; Bae Hwan KIM ; Mee Kyung KIM

Laboratory Animal Research.2010;26(4):385-391. doi:10.5625/lar.2010.26.4.385

In this study, we investigated the in vitro antioxidative effects, antimicrobial activities and single oral dose toxicity of the extracts from Dansam-samultang to evaluate its use as a functional ingredient in cosmetics. In the antioxidative effect, the ethanol extract from Dansam-samultang (DSE) had higher antioxidant values of 92.0% at 1,000 microg/mL than that of water extract from Dansam-samultang (DSW, 86.0%) when evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The superoxide dismutase-like activity of DSW and DSE were 16.3% and 21.3% at 1,000 microg/mL in concentration, respectively. Xanthine oxidase inhibition activity of the DSE was higher 51.5% than that of the DSW (21.4%). This study was also undertaken to test the in vitro antimicrobial activity with the extracts of Dansam-samultang. In general, the DSE showed the significant antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermiders and Escherichia coli. In single oral dose toxicity study, in vivo, there were no differences between control and treated groups in clinical signs, body weight gains, and gross finding. The results indicated that DSE did not show any toxic effects at 10 mL/kg in mice, and the LD50 of DSE was found to be higher than 10 mL/kg in this experiment. In conclusion, the extracts from Dansam-samultang may act as a natural subsistence for functional cosmetics.
Animals ; Biphenyl Compounds ; Body Weight ; Cosmetics ; Escherichia coli ; Ethanol ; Lethal Dose 50 ; Mice ; Picrates ; Staphylococcus ; Staphylococcus aureus ; Superoxides ; Water ; Xanthine Oxidase

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