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Thawing Fresh Frozen Plasma Using a Microwave Oven.

Hyun Sook CHI ; Seog Woon KWON ; Joon NAH

Korean Journal of Blood Transfusion.1995;6(2):217-223.

Thawing fresh frozen plasma(FFP) by waterbath(WB) requires about 30 minutes, which is too slow in emergency situations and carries the risk of bacterial contamination of FFP. To solve these problems, a new thawing method using a microwave oven(MWO) has been developed. Twenty units of equally divided plasma from 10 units of plasma were frozen, stored at -55 degrees C, and thawed in parallel using microwave oven or waterbath. Coagulation factors, plasma proteins and thawing time were measured. Except for antithrombin III(MWO: 85.2+/-6.94%, WB : 90.8+/-9.14%, p<0.05), no significant differences were observed in the 18 other coagulation parameters and the plasma proteins studied. Mean thawing time by MWO was 5.9 minutes per 1 unit, 10.4 minutes per 2 units and 12.5 minutes per 3 units; by WB, it was 19.0, 20.0 and 22.0 minutes, respectively. In conclusion, FFP can be thawed faster using a microwave oven than using 37 degrees C waterbath and the thawed plasma proteins were generally equivalent to those of FFP thawed by waterbath.
Blood Coagulation Factors ; Blood Proteins ; Emergencies ; Microwaves* ; Plasma*

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Analysis of Important Factors Influe_ncing the Filtration Efficiency of Bedside Filter for Leukocyte Removal.

Seog Woon KWON

Korean Journal of Blood Transfusion.1995;6(2):207-216.

Bedside filtration for white celI(WBC) reduction of red cell concentrates(RBCs) has been performed to reduce the workload of blood bank personnel. However, a high incidence of filtration failure with bedside filters was recognized by several investigators. We therefore evaluated the efficiency of the bedside filter under various conditions that might be important variables such as temperature of the blood units, flow rate and possibly, filtration techniques performed. We used 29 bedside filters(RCXL2, Pall Corporation, USA) and total of 58 units of CPDA-1 RBCs for this study. Thirty eight refrigerated RBCs were filtered at fast flow with or without mixing the blood cells in a bag to resuspend the possible blood cell aggregates. And another 20 units were filtered at slow flow(2 hours) with or without mixing after 1 hour incubation at room temperature. Pre-, intra-, and post-filtration blood samples were collected and residual white cells were counted using Nageotte hemocytometer. A higher contamination of white cells was found in the units filtered after mixing at slow flow(mean 2.99 x 105/unit) than at fast flow(mean 1.31 x 10S/unit) and in the units filtered at slow flow without mixing(mean 6.11 x 10S/unit) than with mixing(mean 2.46x 10S/unit). Our data suggested that filtration at slow flow decrease the efficiency of WBC reduction, especially after omitting the mixing process before filtration.
Blood Banks ; Blood Cells ; Filtration* ; Humans ; Incidence ; Leukocytes* ; Research Personnel

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A Family with -D- Phenotype Associated with Fatal Hemolytic Disease of the Newborn.

Kyou Sup HAN ; Hee Chung KIM ; Woo Sup SHIM ; Mi Sook YOON ; Kye Won JOO ; Kwang Soo HAHN ; Bok Yeun HAN

Korean Journal of Blood Transfusion.1995;6(2):201-206.

-D- is a very rare haplotype which determines D without C, c, E or e and exalted D activity. The extremely rare homozygote propositi(-D-/-D-) are usually ascertained through their immune antibodies which react with red cells of all common Rh phenotypes. Authors experienced a woman with -D- phenotype for the first time in Korea. She had a history of abortion and intrauterine fetal death. She delivered a baby with severe hemolytic disease of the newborn at the third pregnancy. In spite of intensive medical interventions, the baby died of hydrops fetalis. An immune antibody to high incidence Rh antigen, namely anti-Hro, was demonstrated in the woman's serum. Family study revealed that all of the family had -D- gene complex and one of her sisters also was -D-homozygote. The sister also had anti-Hro in the serum.
Antibodies ; Female ; Fetal Death ; Haplotypes ; Homozygote ; Humans ; Hydrops Fetalis ; Incidence ; Infant, Newborn* ; Korea ; Phenotype* ; Pregnancy ; Siblings

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Two Cases of Hemolytic Disease of the Newbom due to Anti-E Antibody in Sisters.

Sang Woo KIM ; Jung Ho LEE ; Yong Won PARK ; Bo Moon SIN

Korean Journal of Blood Transfusion.1995;6(2):193-200.

Isoimmune hemolytic disease of the newborn due to anti-E is a disease characterized by the progressive neonatal hyperbilimbinemia and anemia which is caused by the IgG antibody transmitted from the mother to the ferns. Authors have experienced two cases of isoimmune hemolytic disease due to anti-E, who presented with jaundice on the first and the second day of life, respectively. The ABO and Rh blood types of their mother and father were B, CcDee and O, ccDEE, respectively. Both babies showed positive direct Coombs test and the mother showed strong positive indirect Coombs test. Antibody identification tests were made only in the first case and the mother, both of which revealed anti-E antibody. The first case was treated with phototherapy for 3 days and two blood transfusions. The second case was treated with phototherapy for 6 days and two exchange transfusions.
Anemia ; Blood Transfusion ; Coombs Test ; Fathers ; Ferns ; Humans ; Immunoglobulin G ; Infant, Newborn ; Jaundice ; Mothers ; Phototherapy ; Siblings*

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A Family Case of A3B and A3.

Dong Hoon SHIN ; Sung Ran CHO ; Kap Jun YOON ; Deok Woo PARK ; Sang Ok KWON ; Il Gu PARK

Korean Journal of Blood Transfusion.1995;6(2):189-192.

Authors experienced a case of A3B in a 46-year-old patient with liver cirrhosis and two cases of A3 in her children by family study. A3 subgroups were confirmed by delayed and weak positive with anti-A and anti-A,B, negative in anti-A1 lectin, adsorption-elution test, and family study. We report a family case of A3B and A3 with brief review of literatures.
Child ; Humans ; Liver Cirrhosis ; Middle Aged

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A Severe Case of Hemolytic Disease of the Newborn due to Anti-M Antibody.

Kyou Sup HAN ; Jung Hwan CHOI ; Hee Seok KIM ; Dong Hee WHANG

Korean Journal of Blood Transfusion.1996;7(1):71-76.

Anti-M antibody is usually a naturally occurring antibody reacting optimally 4 degrees C and is not considered to be clinically significant. Rarely has anti-M been implicated in hemolytic disease of the newborn(HDN) and the true incidence of HDN due to anti-M has not been well delineated. Authors report the second case of hemolytic disease of the newborn due to anti-M in Korea. A 3-days old baby boy was admitted due to jaundice and severe anemia which were developed at birth. The blood type of his mother was A, CcDEe, Ns, while the blood type of the infant of was A, CcDEe, MNs. The mother's serum had anti-M which wits strongnly positive in room temperature and albumin phase. The reaction was only weakly positive in the antiglobulin phase. Direct antiglobulin test of baby's red cells was negative, while the serum was weakly positive in polyethylene glycol-Coombs test. The antibody was found to be partially IgG through the treatment with dithiothreitol. After an exchange transfusion and phototherapy, the anemia and jaundice were corrected and and the patient discharged at the age of 16.
Anemia ; Coombs Test ; Dithiothreitol ; Humans ; Immunoglobulin G ; Incidence ; Infant ; Infant, Newborn* ; Jaundice ; Korea ; Male ; Mothers ; Parturition ; Phototherapy ; Polyethylene

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Changes in Platelet Activation Markers by Leukocyte-Removal Filters.

Seok Lae CHAE ; Young Joo CHA ; Ae Ja PARK

Korean Journal of Blood Transfusion.1996;7(1):65-70.

Twenty one units of platelet-rich plasma(PRP) were prepared from healthy volunteer blood donors, and each unit of the PRP was divided into two aliquots by using transfer bags. Using SEPACELLTM leukocyte-removal filters, each one aliquot of the PRP was filtered immediately after preparation, and the other aliquot was filtered after a 48-hour storage at a room temperature with continuous agitation. Belbre and after filtration, platelet numbers and two platelet activation markers, CD62 and CD63, were measured using hematologic autoanalyzer and'flow cytometry, respectively. The results were as follows: 1. The platelet numbers in the PRP were reduced after filtration. 2. On the point of the preparation of PRP, the mean percentage of CD63-posititve platelets(s32.86+/- 11.3.5%) was highehr than that of CD62-positive platelets(14.63+/-11.22%). 3. When filtered immediately after preparation of PRP, the CD62-positive platelets were significantly reduced(13.23+/-10.43%), however, CD63-positive platelets were not significantly reduced(29.83+/-11.05%). 4. After 48-hour storage, both two activation markers were increased, and the markers were significantly higher in the PRPs stored after filtration than in those stored without filtration. In conclusion CD63 would be a more sensitive platelet activation marker than CD62, and the platelets expressing CD62 seemed to be removed more than those expressing CD63 during filtration.
Blood Donors ; Blood Platelets* ; Dihydroergotamine ; Filtration ; Healthy Volunteers ; Humans ; Platelet Activation* ; Platelet Count

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Clinical Experiences of Therapeutic Leukapheresis.

Kyou Sup HAN ; Young Kyung LEE

Korean Journal of Blood Transfusion.1996;7(1):57-63.

Therapeutic leukapheresis is a treatment modality which selectively remove abnormal leukocytes and relieve symptoms produced by abnormally high concentration of leukocytes in the blood stream. Authors report the experiences of therapeutic leukapheresis at Seoul National University Hospital during the past 7 years(1988 to 1995). A total of 48 procedures were done for 29 patients(24 males and 5 females). The age distribution was from 16 years to 60 years and mean age was 30 years. The most common diagnosis of patients were acute lymphoblastic leukemia(7/15, 46.7%) and major indications for therapeutic leukapheresis were dyspnea, headache, abdominal pain and mental alteration. The mean leukocyte count before and after leukapheresis were 277,300/micro liter and 220,700/micro liter, respectively(20% decrease, P<0.001). Hemoglobin concentration was also decreased from 7.7g/dL to 7.1g/dL after leukapheresis(P<0.01). The mean number of leukocytes removed per procedure was 3.1x1011. Adverse reactions such as abdominal pain and dizziness were observed in two cases. The leukocyte count was decreased in all patients and improvement of symptoms was observed in four patients. In conclusion, therapeutic leukapheresis is relatively safe and can be used to relieve leukostatic symptoms in leukemic patients.
Abdominal Pain ; Age Distribution ; Diagnosis ; Dizziness ; Dyspnea ; Headache ; Humans ; Leukapheresis* ; Leukocyte Count ; Leukocytes ; Male ; Rivers ; Seoul

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CS-3000 Plus Cell Separator; A-35 Collection Chamber vs PLT-30TM Collection Chamber.

Oh Hun KWON ; Hyun Ok KIM ; Seun Moo LEE ; Hee Bong SHIN ; Chun Hyun NAHM

Korean Journal of Blood Transfusion.1996;7(1):49-55.

BACKGROUND: Recently, it has been established that plateletpheresis needs more efficiency and shorter processing time. Fenwall laboratories developed a new collection chamber for CS-3000 Plus, PLT-30TM collection chamber, which can reduce the processing time with efficient collection. We evaluated the PLT-30TM collection chamber by comparing it with A-35 collection chamber that has been used as a standard collection chamber of CS-3000 Plus us. METHODS: Thirty platelet collection procedures were performed using the CS 3000 Plus with A-35 collection chamber and PLT-30TM collection chamber. The changes of the hematologic parameters between pre- and post-donation in donors and the total platelets yields and the contaminated WBCs in the plateletpheresis products were evaluated. In processing, the yield predictor calibration was adjusted to 1.00 and 1.13 in A-35 and PLT-30TM respectively. Yield predictors of pheresis were the same as 3.5x1011 in both and end point volumes were calculated from the CS-3000 Plus. Processing volume and processing times were compared between A-35 and PLT-30TM groups. RESULTS: With PLT-30TM collection chamber, 3.38+/-0.72x1011/L platelets were harvested, whereas 3.20+/- 0.73x1011/L were collected with A-35 collection chamber, which was not significantly different. But processing time with the PLT-30TM collection chamber was more reduced than that with the A-35 collection chamber by about 20 minutes (PLT-30TM : 88.6+/-8.4 min, A-35 : 106.7+/-11.7min). Collection efficiency of PLT-30TM chamber was 50.7+/-12.5% and that of A-35 chamber was 44.4 + 8.8%. The leukocyte contamination of the platelet concentrates were not statistically different(PLT-30TM: 0.0-3.6x106, A-35 : 0.1-4.1x106). CONCLUSIONS: PLT-30TM collection chamber has the advantages of shortening the donation time and decreasing the processing volume with better collection efficiency and flexibility of platelet concentrate volume.
Blood Component Removal ; Blood Platelets ; Calibration ; Humans ; Leukocytes ; Plateletpheresis ; Pliability ; Tissue Donors

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Correction of Inappropriate Use of Fresh Frozen Plasma by the Hospital Transfusion Control Program.

Kap No LEE ; Chae Seung LIM ; Young Kee KIM

Korean Journal of Blood Transfusion.1996;7(1):39-47.

Hospital transfusion service should be directed toward the optimization of transfusion therapy, but in practice the hospital transfusions, especially of fresh frozen plasma(FFP), were practiced inappropriately against the real need. So we evaluated the usage pattern of fresh frozen plasma in the Capital Armed Forces General Hostal during the period of January through December in 1994. And then, from 1995, after education of the clinician upon the basis of the survey in 1994, we regulated the use of FFP, by adapting the transfusion control program. We compared the result of 1995 to those of 1994, including the total used FFP number, the usage pattem in various disease and the combined use with other blood components. The results were as follow. 1. About 50% of total FFP was used inappropriately at 1994, and the main misuse was done to the patients who were suffering from renal disease and bum. About 14% of FFP usage combined with the packed RBCs was also seemed to be inappropriate in 1994. 2. In 1995, with the transfusion control program, there was 72% fall in the total hospital's usage of FFP and the misused conditions such as bum and nephrotic syndrome were virtually corrected. We concluded that the education, guideline and regulation by the director of the transfusion service could remarkably correct the inappropriate use of FFP without any special costs.
Arm ; Education ; Humans ; Nephrotic Syndrome ; Plasma*

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