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Behavioral Manifestation of Neuropathic Pain Following Sciatic Nerve Section in Rats.

Dong Jin YOO ; Heung Sik NA ; Young Wook YOON

Experimental Neurobiology.2008;17(2):79-86. doi:10.5607/en.2008.17.2.79

The present study was performed to observe the time course of behavioral signs of painful sensations in sciatic neurectomy animal model and to test the effects of sympathectomy and saphenous nerve section on these behavioral signs. Sciatic nerve was ligated and cut at the mid-thigh level under gaseous anesthesia. The application of von Frey filaments to the medial plantar surface of foot revealed weak and long-lasting mechanical allodynia (until end of test period, 20 weeks PO). Acetone application to the plantar surface of foot was used ti measure the sensitivity to cold stimulation. Cold allodynia which is interpreted as increased response to acetone application developed fairly well and lasted the end of test period (20 weeks PO). The cumulative duration of foot lifts off neutral or cold plate was used to test spontaneous, ongoing pain and was increased until 16 weeks PO and 20 weeks PO respectively. These results suggest that sciatic neurectomy which has been widely used as chronic pain model shows behavioral signs suggsting painful sensations except autotomy, which has been used as index of pain in experimental animal. Surgical sympathectomy performed 1 week after sciatic neurectomy partially reduced the behavioral signs of mechanical allodynia and cold allodynia, suggesting behavioral changes developed following section of sciatic nerve was partially sympathetic dependent. Saphenous nerve section 1 week after sciatic neurectomy almost completely reduced mechanical allodynia and cold allodynia, but did not change spontaneous, ongoing pain. These results suggest that evoked responses such as mechanical and cold allodynia are mediated by saphenous nerve activity and activating and/or maintaining mechanisms of spontanous, ongoing pain and evoked pain may be different.
Acetone ; Anesthesia ; Animals ; Chronic Pain ; Cold Temperature ; Foot ; Hyperalgesia ; Models, Animal ; Neuralgia ; Rats ; Sciatic Nerve ; Sensation ; Sympathectomy

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Effects of Armeniacae Semen and Amygdalin on the Lipopolysaccaride-induced Prostaglandin E2 Synthesis and Nitric Oxide Production in Mouse BV2 Microglial Cells.

Hyung Jin JUNG ; Young Sick KIM ; Mal Soon SHIN ; Chang Ju KIM ; Youn Sub KIM

Experimental Neurobiology.2008;17(2):71-78. doi:10.5607/en.2008.17.2.71

Armeniacae semen has been used in traditional medicine for the treatment of pain and inflammatory diseases. Amygdalin is the major compound of Armeniacae semen, and it is used for treatment of pain and cancers. In the present study, we compared the effects of aqueous extract of Armeniacae semen and a solution of amygdalin extracted from Armeniacae semen on the lipopolysaccharide (LPS)-stimulated cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) mRNA expressions in mouse BV2 microglial cells. We also compared the effects of these compounds on the prostaglandin E2 synthesis and the nitric oxide production in mouse BV2 microglial cells. In the present results, Armeniacae semen and amygdalin suppressed prostaglandin E2 synthesis and nitric oxide production by inhibiting the LPS-induced enhancement of COX-2 mRNA and iNOS mRNA expressions in mouse BV2 cells. For the COX-1 expression, Armeniacae semen showed more potent suppression effect compared to the amygdalin. However, amygdalin more potently suppressed the LPS-induced COX-2 mRNA expression compared to aqueous extract of Armeniacae semen. In the case of iNOS mRNA expression, Armeniacae semen and amygdalin showed similar suppressing effects. For the LPS-induced PGE2 synthesis, amygdalin showed more potent suppressing effect, meanwhile, Armeniacae semen and amygdalin showed similar suppressing effect on NO production. Based on the present results, amygdalin may exert anti-inflammatory and analgesic effect though mainly the inhibition of COX-2 pathway, in contrast Armeniacae semen may exert such effect though both the inhibition of COX-2 and iNOS pathways.
Amygdalin ; Animals ; Cyclooxygenase 1 ; Cyclooxygenase 2 ; Dinoprostone ; Medicine, Traditional ; Mice ; Nitric Oxide ; Nitric Oxide Synthase Type II ; Prostaglandin-Endoperoxide Synthases ; RNA, Messenger ; Semen

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Protective Effect of Orally Administrated Dandelion Against Carrageenan-induced Ankle Arthritis in Rats.

Bong Jun SUR ; Yoon Jeong KOH ; Hee Don CHOI ; Insop SHIM ; Hyejung LEE ; Dae Hyun HAHM

Experimental Neurobiology.2008;17(2):63-69. doi:10.5607/en.2008.17.2.63

Dandelion (DA) possesses the therapeutic ability to eliminate heat and alleviating swelling, choleresis, dieresis, and inflammation. In order to investigate the anti-arthritic effect of DA, several behavioral parameters such as paw volume, squeaking score, and weight distribution ratio were investigated in a carrageenan-induced arthritis rat model. At the maximum severity of arthritis, the daily administration of DA was initiated and lasted for 9 days. The therapeutic effects of DA were observed on 9th day after the arthritis induction, as compared to saline-treated control group. Oral administration of DA significantly alleviated apparent symptoms of paw volume, squeaking score, and weight distribution ratio in rats. In conclusion, DA was found to be effective in alleviating the inflammatory response and thus arthritic symptoms in carrageenan-induced arthritic rats.
Administration, Oral ; Animals ; Ankle ; Arthritis ; Carrageenan ; Hot Temperature ; Inflammation ; Rats ; Taraxacum

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Protective Effect of Shenqi-wan on Traumatic Brain Injury-induced Delayed Apoptosis in Rat Hippocampal Dentate Gyrus.

Oh Bong KWON ; Hyung Ho LIM ; Yun Kyung SONG ; Jin Woo LEE ; Young Sick KIM ; Chang Ju KIM

Experimental Neurobiology.2008;17(2):55-62. doi:10.5607/en.2008.17.2.55

Shenqi-wan, Oriental herbal medicine formulation, has been traditionally used for delayed mental and physical development in children, complications of diabetes, and glomerulonephritis. In the present study, the protective effect of the aqueous extract of Shenqi-wan against traumatic brain injury (TBI) in the rat hippocampal dentate gyrus was investigated. For this study, step-down avoidance task, terminal deoxynuclotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, Bax immunohistochemistry, and 5-bromo-2'-deoxyuridine (BrdU) immunohistochemistry were conducted. In the present results, it was shown that apoptotic cell death and cell proliferation in the dentate gyrus were significantly increased following TBI in rats and that the aqueous extract of Shenqi-wan suppressed the TBI-induced increase in apoptosis and cell proliferation in the dentate gyrus. Based on the present results, it is possible that the aqueous extract of Shenqi-wan has a neuroprotective effect on TBI-induced neuronal cell death.
Animals ; Apoptosis ; Brain ; Brain Injuries ; Bromodeoxyuridine ; Cell Death ; Cell Proliferation ; Child ; Dentate Gyrus ; Glomerulonephritis ; Herbal Medicine ; Humans ; Immunohistochemistry ; Neurons ; Neuroprotective Agents ; Rats ; Transferases

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The Central and Peripheral Production of Pro-inflammatory Cytokine, IL-1b after Immune and Stress Stimulation in Rats.

Eun Yee JUNG ; Kyung Soo KIM ; Insop SHIM

Experimental Neurobiology.2008;17(2):47-54. doi:10.5607/en.2008.17.2.47

Interleukin-1beta (IL-1beta), one of the pro-inflammatory cytokines, acts as an endogenous pyrogen and is an important mediator of behavioral and physiological responses to immune stimulation as well as exposure to stressors. The objective of the present study was to examine the pattern of central or peripheral IL-1beta response to lipopolysaccharide (LPS) or exposure to the foot shock stress (FS) in rats. After treatment of LPS (100microgram/kg) or exposure to the FS [ten times (0.8 mA) foot shocks for 5 sec each and 90 sec interval], body temperature and IL-1beta levels in plasma, spleen and brain were measured. Both LPS and FS stimuli elicited increased body temperature but showed different patterns of peripheral IL-1beta levels. LPS produced a widespread increase in IL-1beta levels in the plasma, spleen and brain, whereas FS produced a significant increase in IL-1beta levels only in the brain regions but not in plasma and spleen. The present study suggests that IL-1beta is, centrally or peripherally in different patterns, regulated by immune stimulation or exposure to stressors and IL-1beta plays an important role in mediating responses of sickness-like behaviors induced by immune stimuli or stressors.
Animals ; Body Temperature ; Brain ; Cytokines ; Foot ; Interleukin-1 ; Interleukin-1beta ; Negotiating ; Plasma ; Rats ; Shock ; Spleen

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Striatal CREB Phosphorylation Following Cued/Response Learning in the Water Maze Differ in Two Inbred Strains of Mice.

Yoo Kyeong HWANG ; Jung Soo HAN

Experimental Neurobiology.2008;17(2):41-46. doi:10.5607/en.2008.17.2.41

Much evidence shows that the hippocampus and striatum play roles as important neural substrates for spatial/place and cued/response learning, respectively. This experiment was conducted to investigate the engagement of the striatum in cued/response learning. The engagement of the striatum was assessed after either place or cue training by determining levels of cAMP response element-binding protein (CREB) and phosphorylated CREB (pCREB) in these two mouse strains. Results revealed that striatal CREB levels in both strains of mice were not significantly increased after cued/response learning comparing to place training mediated by the hippocampus. However, striatal pCREB of DBA/2 mice was significantly higher after cued/response training in comparison to place learning, while striatal pCREB levels on C57BL/6 mice did not differ in cued learning versus place learning. These findings indicate that striatal pCREB, specifically associated with cued/response learning, is closely tied to differences in cued/responses strategy preference between C57BL/6 and DBA/2 mice.
Animals ; Cues ; Cyclic AMP Response Element-Binding Protein ; Hippocampus ; Hypogonadism ; Learning ; Maze Learning ; Mice ; Mice, Inbred Strains ; Mitochondrial Diseases ; Ophthalmoplegia ; Phosphorylation

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Classification of BMI Control Commands Using Extreme Learning Machine from Spike Trains of Simultaneously Recorded 34 CA1 Single Neural Signals.

Youngbum LEE ; Hyunjoo LEE ; Yiran LANG ; Jinkwon KIM ; Myoungho LEE ; Hyung Cheul SHIN

Experimental Neurobiology.2008;17(2):33-39. doi:10.5607/en.2008.17.2.33

A recently developed machine learning algorithm referred to as Extreme Learning Machine (ELM) was used to classify machine control commands out of time series of spike trains of ensembles of CA1 hippocampus neurons (n=34) of a rat, which was performing a target-to-goal task on a two-dimensional space through a brain-machine interface system. Performance of ELM was analyzed in terms of training time and classification accuracy. The results showed that some processes such as class code prefix, redundancy code suffix and smoothing effect of the classifiers' outputs could improve the accuracy of classification of robot control commands for a brain-machine interface system.
Aniline Compounds ; Animals ; Brain-Computer Interfaces ; Hippocampus ; Learning ; Neural Prostheses ; Neurons ; Rats ; Machine Learning

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Interactions of Dopamine D1 and N-methyl-D-Aspartate Receptors are Required for Acute Cocaine-Evoked Nitric Oxide Efflux in the Dorsal Striatum.

Dong Kun LEE ; Sung Min AHN ; Yoon Bo SHIM ; Wei Choon Alvin KOH ; Insop SHIM ; Eun Sang CHOE

Experimental Neurobiology.2011;20(2):116-122. doi:10.5607/en.2011.20.2.116

Alterations in nitric oxide (NO) release in response to psychostimulants in the striatum cause a plastic change contributing to the development and expression of addiction. In this study, regulation of NO efflux evoked by acute cocaine in the dorsal striatum was investigated using real-time detection of NO in vivo. We found that acute systemic injection of cocaine (20 mg/kg) increased NO efflux, which was reduced by the intrastriatal infusion of the dopamine D1 receptor antagonist, SCH23390 (7.5 nmol), and the dopamine D2 receptor agonist, quinpirole (5 nmol). Increased levels of NO efflux by acute cocaine were also reduced by the intrastriatal infusion of the N-methyl-D-aspartate (NMDA) receptor antagonists, MK801 (2 nmol) and AP5 (2 nmol). These findings suggest that interactions of dopamine D1 receptors and NMDA receptors after acute exposure to cocaine participate in the upregulation of NO efflux in the dorsal striatum.
Benzazepines ; Cocaine ; Dizocilpine Maleate ; Dopamine ; Glutamic Acid ; N-Methylaspartate ; Nitric Oxide ; Plastics ; Quinpirole ; Receptors, Dopamine D1 ; Receptors, Dopamine D2 ; Receptors, N-Methyl-D-Aspartate ; Up-Regulation

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Simple and Novel Three Dimensional Neuronal Cell Culture Using a Micro Mesh Scaffold.

Sang Jin YOO ; Jongmin KIM ; Chang Soo LEE ; Yoonkey NAM

Experimental Neurobiology.2011;20(2):110-115. doi:10.5607/en.2011.20.2.110

Conventional method of cell culture studies has been performed on two-dimensional substrates. Recently, three-dimensional (3D) cell culture platforms have been a subject of interest as cells in 3D has significant differences in cell differentiation and behavior. Here we report a novel approach of 3D cell culture using a nylon micro mesh (NMM) as a cell culture scaffold. NMM is commonly used in cell culture laboratory, which eliminates the requirement of special technicality for biological laboratories. Furthermore, it is made of a micro-meter thick nylon fibers, which was adequate to engineer in cellular scales. We demonstrate the feasibility of the NMM as a 3D scaffold using E18 rat hippocampal neurons. NMM could be coated with cell adhesive coatings (polylysine or polyelectrolyte) and neurons showed good viability. Cells were also encapsulated in an agarose hydrogel and cultured in 3D using NMM. In addition, the 3D pattern of NMM could be used as a guidance cue for neurite outgrowth. The flexible and elastic properties of NMMs made it easier to handle the scaffold and also readily applicable for large-scale tissue engineering applications.
Adhesives ; Animals ; Cell Culture Techniques ; Cell Differentiation ; Cues ; Hydrogel ; Neurites ; Neurons ; Nylons ; Rats ; Sepharose ; Tissue Engineering ; Weights and Measures

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Protective Effect of Coriolus versicolor Cultivated in Citrus Extract Against Nitric Oxide-Induced Apoptosis in Human Neuroblastoma SK-N-MC Cells.

Byung Chul KIM ; Youn Sub KIM ; Jin Woo LEE ; Jin Hee SEO ; Eun Sang JI ; Hyejung LEE ; Yong Il PARK ; Chang Ju KIM

Experimental Neurobiology.2011;20(2):100-109. doi:10.5607/en.2011.20.2.100

Nitric oxide (NO) is a reactive free radical and a messenger molecule in many physiological functions. However, excessive NO is believed to be a mediator of neurotoxicity. The medicinal plant Coriolus versicolor is known to possess anti-tumor and immune-potentiating activities. In this study, we investigated whether Coriolus versicolor possesses a protective effect against NO donor sodium nitroprusside (SNP)-induced apoptosis in the human neuroblastoma cell line SK-N-MC. We utilized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme activity assay in SK-N-MC cells. MTT assay showed that SNP treatment significantly reduces the viability of cells, and the viabilities of cells pre-treated with the aqueous extract of Coriolus versicolor cultivated in citrus extract (CVEcitrus) was increased. However, aqueous extract of Coriolus versicolor cultivated in synthetic medium (CVEsynthetic) showed no protective effect and aqueous citrus extract (CE) had a little protective effect. The cell treated with SNP exhibited several apoptotic features, while those pre-treated for 1 h with CVEcitrus prior to SNP expose showed reduced apoptotic features. The cells pre-treated for 1 h with CVEcitrus prior to SNP expose inhibited p53 and Bax expressions and caspase-3 enzyme activity up-regulated by SNP. We showed that CVEcitrus exerts a protective effect against SNP-induced apoptosis in SK-N-MC cells. Our study suggests that CVEcitrus has therapeutic value in the treatment of a variety of NO-induced brain diseases.
Apoptosis ; Blotting, Western ; Brain Diseases ; Caspase 3 ; Cell Line ; Citrus ; DNA Fragmentation ; DNA Nucleotidylexotransferase ; Flow Cytometry ; Humans ; Indoles ; Neuroblastoma ; Nitric Oxide ; Nitroprusside ; Plants, Medicinal ; Tetrazolium Salts ; Thiazoles ; Tissue Donors

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