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Effects of hyperbaric oxygen on plasma endothelin and nitric oxide in patients with intrauterine growth retardation

Fang LIU ; Guirong LYU ; Yuan CAO ; Hengyuan AN

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(1):83-86. doi:10.3760/cma.j.issn.1009-6906.2020.01.022

Objective:To investigate the effect of hyperbaric oxygen on plasma endothelin-1 (ET-1) and nitric oxide (NO) in patients with intrauterine growth retardation (IUGR) as well as the clinical efficacy.Methods:A total of 90 IUGR patients, treated in the First Affiliated Hospital of Zhengzhou University from January 2018 to May 2019, were selected and divided into the observation group and the control group according to the double-blind lottery method, with 45 patients in each group. The patients in the control group were only given routine treatment, while those in the observation group were treated with hyperbaric oxygen combined with the routine treatment as same as the control group. The therapeutic effects of the two groups were observed and recorded. The level of ET-1 was analyzed by enzyme linked immunosorbent assay, and the level of NO was tested by nitrate reductase colorimetry. The values of peak-to-diastolic ratio (S/D), resistant index (RI), and pulsation index (PI) at the end of umbilical artery vasoconstriction were detected by ultrasonic doppler umbilical cord blood flow analyzer. The levels of plasma TXB2 and 6-Keto-PGF1α were detected by radioimmunoassay.Results:After 3 weeks treatment, the total effective rate of the observation group (95.56%) was significantly higher than that of the control group (77.78%) ( χ2=7.168, P<0.05); compared with those before treatment, the levels of ET-1 and NO of both groups were significantly up-regulated; the ET-1 level of the observation group after treatment was significantly lower than that of the control group, while the NO level was significantly higher ( P<0.05). After treatment, the values of S/D, RI, and PI at the end of umbilical artery vasoconstriction of both the groups after treatment were all lower than those before treatment, especially the values of S/D, RI, and PI of the observation group after treatment were significantly lower than those of the control group ( P<0.05). Compared with those before treatment, the levels of patients′ plasma TXB2 and 6-keto-PGF1α of both the groups were significantly up-regulated; the level of patients′ plasma TXB2 of the observation group after treatment was significantly lower than that of the control group, while the level of 6-keto-PGF1α was significantly increased( P<0.05 or P<0.01). Conclusion:Hyperbaric oxygen could significantly improve the clinical effect on IUGR, and improve the fetal development. This treatment, therefore, is worthy of popularization and application in clinic.

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Empirical study of standardized wounded soldier grading evaluation indicator system in training of pre-hospital care for naval battle injury

Siyue ZHU ; Yixin WANG ; Yan HUANG ; Li GUI

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(1):32-36. doi:10.3760/cma.j.issn.1009-6906.2020.01.010

Objective:To apply the standardized wounded soldier (SWS) grading evaluation indicator system in naval troops and naval colleges of China respectively to test its effectiveness.Methods:A total of 30 soldiers from 15 naval troops were trained as basic-level SWS, and 6 students from a naval college were trained as professional-level SWS. The indicator system was verified in the form of knowledge questionnaire and quality report. Convenience sampling was used to select medics, instructors and SWS as subjects for evaluation.Results:The reliability, discrimination and difficulty of the knowledge questionnaire were good, and the reliability and validity of quality report were good. The general evaluation of the professional-level SWS was better than that of the basic-level SWS.Conclusion:SWS grading evaluation indicator system is suitable and feasible for reasonably evaluating the simulation quality of SWS and stepping up CPLA military training in real combat condition, and is conducive to promoting the popularization and application of SWS in military medical simulation training.

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Effects of hyperbaric oxygen combined with selegiline on motor function, serum miR-124, and IGF-1 in patients with Parkinson′s disease

Yumin CHEN ; Xuan ZHANG ; Qingyan FENG ; Bingwang YAN ; Jingjing WEI ; Liying ZHAO ; Wenting ZHAO ; Xinyan YI

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(2):200-204. doi:10.3760/cma.j.cn311847-20191018-00271

Objective:To investigate the effects of hyperbaric oxygen (HBO) combined with selegiline on motor function, serum microRNA-124 (miR-124), insulin-like growth factor-1 (IGF-1) in patients with Parkinson′s disease (PD).Methods:A total of 120 PD patients, admitted in the Department of Neurology of Jining No.1 People′s Hospital from the March of 2017 to the March of 2019, were selected and divided into control group and HBO group according to the random number table method with each group consisting of 60 patients. The patients in the control group received levodopa and selegiline treatment, while the patients of HBO group were treated with HBO combined with the same medication of the control group. After three courses of treatment, clinical symptoms of the two groups were observed; motor function was assessed by the unified Parkinson′s disease rating scale (UPDRS); non-motor function was assessed by the PD non-motor symptom questionnaire (NMSQuest) and the Parkinson′s disease sleep scale (PDSS); the levels of serum oxidative stress index, inflammatory factor, IGF-1 and miR-124 before and after treatment were compared.Results:After 30 d treatment, the incidences of mental disorders, sensory disturbances, autonomic nervous dysfunction and sleep disorders of the two groups were significantly lower than those before treatment, and those of HBO group after treatment were significantly lower than those of the control group ( P<0.01). The UPDRS II and UPDRS III scores of both groups after treatment were significantly lower than those before treatment ( P<0.05). The UPDRS II and UPDRS III scores of the HBO group after treatment were significantly lower than those of the control group ( P<0.05 or P<0.01). Compared with those before treatment, the NMSQuest score of both two groups decreased and the PDSS score of both two groups increased. After treatment, the NMSQuest score of the HBO group was significantly lower than that of the control group; while the PDSS score of the HBO group was significantly higher than that of the control group ( P<0.05 or P<0.01). Compared with those before treatment, the levels of hs-CRP, IL-6, and MDA of the HBO group were significantly lower than those of the control group, while the SOD was significantly higher than that of the control group ( P<0.05). After treatment, the relative quantitative expressions of miR-124 and the levels of IGF-1 of both two groups were significantly higher than those before treatment, and the relative quantitative expressions of miR-124 and the levels of IGF-1 of the HBO group was significantly higher than those of the control group ( P<0.05 or P<0.01). Conclusion:HBO combined with selegiline can significantly improve the oxidative stress disorder in PD patients, promote the expressions of miR-124 and IGF-1, strengthen neuroprotective effects, and significantly improve motor function and non-motor disorders, so as to improve the therapeutic effect.

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Clinical study of hyperbaric oxygen combined with medication on postherpetic neuralgia

Dezhi ZHANG ; Xiaorong LIU ; Meigui QIAN

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(2):192-195,f4. doi:10.3760/cma.j.cn311847-20190115-00007

Objective:To investigate the clinical curative effect of hyperbaric oxygen (HBO) on postherpetic neuralgia (PHN).Methods:A total of 80 patients, who were treated in Dermatology Department of Naval Anqing Hospital from the August of 2016 to the August of 2018 and met the inclusion criteria, were enrolled and randomly divided into control group and study group according to the random number table method, with 40 cases per group. The patients in the control group were treated with modified Tuolixiaodu powder. The patients in the study group were treated with HBO combined with modified Tuolixiaodu powder. The efficacy, visual analogue scale (VAS) score, quality of life score and brain-derived neurotrophic factor (BDNF) expression of the two groups were observed.Results:The effective rate of the study group was 95.0% (38/40), and that of the control group was 70.0% (28/40). The difference between the two groups was statistically significant ( P=0.019). The efficacy of the study group was obviously better, and the difference between the two groups was statistically significant ( P=0.032). Compared with those before treatment, the VAS scores of both groups after treatment decreased, with statistically significant difference ( P<0.05). Compared with those before treatment, the scores of the life quality of both groups obviously increased, with statistically significant difference ( P<0.05). The patients of the study group gained better curative effects, e. g. at their work, study, and daily life, and the two groups had significant differences ( P<0.05). The BDNF expression levels in the study group and the control group both increased after treatment, with statistically significant difference ( P<0.05). Conclusion:The combination of HBO to the treatment of modified Tuolixiaodu powder on PHN can improve its action of replenishing qi and blood, promoting blood circulation, and relieving pain by dispersing stasis, to improve patients' life quality and the expression level of BDNF.

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Inhibitory effect of hyperbaric oxygen on growth of transplanted nasopharyngeal carcinoma in nude mice and possible mechanism involved

Yan ZHANG ; Julin LI ; Jian ZHAO ; Ting WANG ; Junli WANG

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(2):234-238. doi:10.3760/cma.j.cn311847-20191030-00291

Objective:To investigate the inhibitory effect of hyperbaric oxygen (HBO) on the growth of transplanted nasopharyngeal carcinoma (NPC) in nude mice and its mechanism.Methods:After the transplanted tumor model of human NPC cell line (CNE-2) in nude mice was established, twenty tumor-bearing nude mice were divided into control and HBO group by using a random number table method, each with 10 mice. The nude mice in the HBO group were exposed to one-hour-session of HBO a day for two consecutive weeks. After two weeks treatments, the mice in both groups were sacrificed, and tumor mass and volume were measured to calculate the tumor growth inhibition rate. Real-time polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in transplanted tumor cells. Immunohistochemistry analysis was used to detect the expression levels of VEGF and bFGF proteins and tumor microvessel density (MVD). Spearman correlation analysis was used to assess the correlation between VEGF/bFGF expressions and MVD.Results:After 14 consecutive days of HBO treatment, the inhibition rates of tumor mass and tumor volume in the transplanted tumor model mice of the HBO group were respectively 43.12% and 43.06%, both were significantly lower than those of the control group ( P<0.01). The expression levels of VEGF mRNA and bFGF mRNA in the transplanted tumor tissue of the HBO group were significantly lower than those of the control group ( P<0.01). Immunohistochemistry detection results showed that, regarding the VEGF and bFCG expressions in the cytoplasm of tumor cells, the cytoplasmic staining of positive cells was brownish yellow, and the nucleus staining was light blue. The expression levels of VEGF and bFGF in the HBO group were significantly lower than those in the control group ( P<0.01). The immunohistochemistry detection results also indicated that, regarding the CD-34 protein expression in vascular endothelial cells, the staining was brownish yellow. The microvessels were of different shapes and sizes, and no regular vascular lumen was formed. The MVD value of the HBO group (33.16±4.89) was significantly lower than that of the control group (45.69±5.26), with statistical significance ( P<0.01). Spearman correlation analysis demonstrated that VEGF protein expression was positively correlated with MVD expression ( r=0.862, P=0.001), and bFGF protein expression was also positively correlated with MVD expression ( r=0.745, P=0.013). Conclusion:HBO has a significant growth inhibitory effect on the transplanted NPC in nude mice, the mechanism of which might be related to the reduction of the expression levels of VEGF and bFGF.

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Efficacy analysis of endovascular therapy combined with hyperbaric oxygen intervention in acute anterior circulation macrovascular occlusive stroke

Dongzhang XUE ; Jincheng CHENG ; Shiquan YANG ; Yiqun WANG ; Tian XIA ; Hanchun WANG ; Xiaolin LI ; Jingjing LIU ; Rui AI ; Shaodan WU ; Bensong YU

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(2):175-179. doi:10.3760/cma.j.cn311847-20191115-00321

Objective:To explore efficacy and safety of endovascular therapy combined with hyperbaric oxygen (HBO) intervention in acute anterior circulation macrovascular occlusion stroke.Methods:A total of 150 patients with acute anterior circulation macrovascular occlusive stroke admitted in No.902 Hospital of the CPLA Joint Logistic Support Force were selected and randomly divided into endovascular treatment group and combined treatment group (endovascular+ HBO). Endovascular therapy was adopted in the treatment of all patients. Modified thrombolytic flow grading (mTICI) was used to evaluate vascular recanalization after operation, and the incidences of complications of hemorrhage transformation and vascular re-occlusion were recorded. National institutes of health stroke scale (NIHSS) was used to evaluate the neurological functions on admission, at 24 hours after the operation, and one course after HBO therapy. The prognosis was evaluated by observing the changes of blood homocysteine (HCY) and high-sensitivity C-reactive protein (hs-CRP) levels before and after treatment. The patients had been followed up by the modified Rankin Scale (mRS) for 90 days. At the same time, 75 patients with acute anterior circulation macrovascular occlusive stroke without taking endovascular treatment were selected as the control group.Results:Among 150 patients, 126 cases had a grade of mTICI≥2b, while 24 cases had a grade of mTICI<2b. Postoperative vascular re-occlusion occurred in 3 cases, and symptomatic intracranial hematoma occurred in 6 cases. The NIHSS score at 24 hours after the operation was significantly lower than that on admission, and the NIHSS score was further improved after HBO treatment. Ninety days after the operation, mRS showed 98 cases of good prognoses, while 52 cases of poor prognoses. The levels of HCY and hs-CRP were significantly lower than those before the treatment.Conclusion:Endovascular therapy for acute anterior circulation macrovascular occlusive stroke is safe and effective. Postoperative HBO therapy can significantly improve the prognosis and the quality of life of patients.

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Effects of hyperbaric oxygen therapy on differentiation of endogenous neural stem cells in rats with focal cerebral ischemia at different stages

Yongjun MAI ; Xiang LI ; Liangzuo ZHENG ; Hong YANG ; Xiaomou WEI ; Lianbi XUE

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(2):224-229. doi:10.3760/cma.j.cn311847-20190918-00228

Objective:To observe the differentiation of endogenous neural stem cells (NSCs) in the ischemic brain of rats with focal cerebral ischemia at different stages with various treatments.Methods:The model of focal cerebral ischemia was established by middle cerebral artery occlusion (MCAO). A total of 105 rats were randomly divided into sham-surgery group (SS), ischemia-reperfusion group (IR), receiving hyperbaric oxygen (HBO) 1 day after modeling group (IR1d+ HBO), receiving HBO 7 days after modeling group (IR7d+ HBO group), receiving HBO 14 days after modeling group (IR14d+ HBO group), and receiving HBO 28 days after modeling group (IR28d+ HBO group). At the 2nd, 4th, 6th and 8th week after modeling, the cells of newly generated neurons and astrocyte in the ischemic area were positive in both immunofluorescence BrdU/β-tubulin and BrdU/glial fibrillary acidic protein (GFAP) test were detected.Results:Two weeks after modeling, the expressions of BrdU/β-tubulin were 11.40±1.52 in the SS group, 36.60±2.51 in the IR group, 48.60±3.21 in the IR1d+ HBO group, and 42.60±2.30 in the IR7d+ HBO group; four weeks after modeling, it was 12.20±1.92 in the SS group, 26.20±2.28 in the IR group, 39.80±2.17 in the IR1d+ HBO group, 33.20±1.92 in the IR7d+ HBO group, 29.20±1.48 in the IR14d+ HBO group. Six weeks after modeling, it was 12.40±1.67 in the SS group, 18.60±1.82 in the IR group, 30.20±2.49 in the IR1d+ HBO Group, 25.40±2.79 in the IR7d+ HBO group, 21.40±2.50 in the IR14d+ HBO group, 19.60±1.67 in the IR28d+ HBO group. Eight weeks after modeling, it was 11.80±1.64 in the SS group, 16.40±2.07 in the IR group, 24.40±1.95 in the IR1d+ HBO group, 21.20±1.48 in the IR7d+ HBO group, 18.40±1.67 in the IR14d+ HBO group, and 15.60±1.82 in the IR28d+ HBO group. The differences in the expressions of BrdU/β-tubulin between these groups were statistically significant ( P<0.01). Compared with the SS group, the expression of BrdU/β-tubulin increased in the IR group at each time point ( P<0.05). The expressions of BrdU/β-tubulin in the IR1d+ HBO and the IR7d+ HBO were higher than those in the IR group at each time point; while there were statistical differences comparing those in the IR14d+ HBO group and the IR28d+ HBO group with those in the IR group at each time point ( P>0.05). The expressions of BrdU/β-tubulin were at the highest level at the second week in all the groups except the SS group ( P<0.05). (2) Two weeks after modeling, the expression of BrdU/GFAP was 22.60±1.82 in the SS group, 59.00±3.67 in the IR group, 50.60±2.51 in the IR1d+ HBO group, and 55.20±2.58 in the IR7d+ HBO group. Four weeks after modeling, it was 22.20±1.48 in the SS group, 45.00±2.24 in the IR group, 35.80±1.64 in the IR1d+ HBO group, 40.20±2.16 in the IR7d+ HBO group, and 44.60±2.51 in the IR14d+ HBO group. Six weeks after modeling, it was 22.80±1.64 in the SS group, 26.68±1.78 in the IR group, 27.40±1.67 in the IR1d+ HBO group, 28.40±1.51 in the IR7d+ HBO group, 26.20±1.78 in the IR14d+ HBO group, and 26.20±1.48 in the IR28d+ HBO group. Eight weeks after modeling, it was 21.60±1.81 in the SS group, 21.40±1.14 in the IR group, 24.00±1.58 in the IR1d+ HBO group, 24.80±1.92 in the IR7d+ HBO group, 23.40±1.67 in the IR14d+ HBO group, and 22.20±1.30 in the IR28d+ HBO group. At each time point, the differences of BrdU/GFAP expression between each group were statistically significant ( P<0.05). Furthermore, the BrdU/GFAP expression in the IR group was higher than that in the SS group at each time point except the 8th week. The expressions of BrdU/GFAP in the IR1d+ HBO group and the IR7d+ HBO group were higher than those in the IR group at the 2nd and the 4th weeks ( P<0.05). There was no statistically significant difference in the expressions of BrdU/GFAP between the IR1d+ HBO group and the IR7d+ HBO group at the 6th and the 8th week( P>0.05). There was no statistically significance comparing the expressions of BrdU/GFAP in the IR14d+ HBO and the IR 28d+ HBO group respectively with those in the SS group( P>0.05) at each time point. All the expressions of BrdU/GFAP in each group reached the highest level at the 2nd week ( P<0.05). Conclusion:In the early and the recovery period, HBO can promote the differentiation of NSCs in the ischemic areas into neurons and inhibit its differentiation into astrocytes. An early long-term treatment of HBO can maintain NSCs differentiation in the ischemic area into neurons for a long time.

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Investigation and cause analysis of seasickness in participants of along oceangoing voyage mission

Jinping WANG ; Haiyan LI ; Ruilin YU ; Feifei HUANG ; Huohong QIAN

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(1):22-24,28. doi:10.3760/cma.j.issn.1009-6906.2020.01.007

Objective:To analyze the causes of seasickness in the mission participants of the hospital ship Peace Ark during along oceangoing voyage, so as to provide a reference basis for optimizing mission participants drafting and reducing the incidence of seasickness in future.Methods:We recruited 260 volunteers from the mission participants of the hospital ship Peace Ark, and carried out a survey with a self-made questionnaire. The questionnaire includes two parts: Part 1 collected the demographic information, e. g. sex, age, body mass, and smoking history, and Part 2 was for analyzing the factors that might affect the occurrence of seasickness, e. g. the history of participating oceangoing missions, the current onboard job, the location of living cabin, the history of daily physical exercise before the mission, the history of daily physical exercise during the current mission, etc. A total of 251 responses were collected. All the data were analyzed by SPSS19.0 software.Results:Univariate analysis showed that there were significant correlations between seasickness and sex, weight, smoking, the history of participating oceangoing missions, the location of living cabin, and the current onboard job ( P<0.05). The results of multivariate regression analysis showed that the sex of female and the history of participating any oceangoing missions were the risk factors of seasickness ( P<0.05). The analysis of threshold saturation effect showed that from the second stage of the voyage on, the incidence of seasickness decreased by 0.51% after each stage. Conclusion:When drafting participants for oceangoing missions, the basic conditions of participants should be comprehensively evaluated. Those who were easily suffering from seasickness should be allocated to the living cabins as much at the middle or lower part of the ship as possible. The mission participants should pay attention to physical exercise, well-balanced nutritional intake, and the adaptive training of anti-seasickness.

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Effect of hyperbaric oxygen combined with medication on postoperative delirium after spine surgery

Zaiqing ZHANG ; Yulong WU ; Bo CHEN

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(1):50-52. doi:10.3760/cma.j.issn.1009-6906.2020.01.014

Objective:To investigate the effect of hyperbaric oxygen(HBO) combined with medication in the treatment of postoperative delirium after spine surgery.Methods:A total of 124 patients with postoperative delirium after spine surgery hospitalized from December 2012 to September 2018 were divided into the experimental group and the control group according to the preferences of patients or their relatives. Each group consisted of 62 patients. The control group received conventional medication, while the experimental group received HBO combined with conventional medication. After 7 days treatment, the clinical effects were evaluated by Brief Psychiatric Rating Scale(BPRS) and Clinical Global Impression Scale (CGIS).Results:Before the treatments, there was no statistical significance of BPRS and CGIS between the experimental group and the control group ( P>0.05). After 7 days treatment, the BPRS (20.2±2.7) and CGIS (2.05±0.73) of experimental group were significantly lower than those of the control group (29.7±4.7 and 3.99±1.70); the difference between the two groups was statistically significant ( P<0.05). Conclusion:HBO combined with medication in the treatment of postoperative delirium after spine surgery could better relieve clinical symptoms, therefore, improve patients′ life quality.

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Clinical effects of hyperbaric oxygen combined with citalopram on sleep disorders in patients with depression

Huijun DUAN ; Pingzhi WANG ; Aiping LI ; Xiaoqing CHEN

Chinese journal of nautical medicine and hyperbaric medicine.2020;27(1):46-49. doi:10.3760/cma.j.issn.1009-6906.2020.01.013

Objective:To investigate the clinical effect and action mechanism of hyperbaric oxygen (HBO) combined with citalopram on sleep disorders.Methods:One hundred and forty patients with depression suffering from sleep disorder, admitted to Shanxi Bethune Hospital from March 2016 to June 2019, were enrolled and randomly divided into the observation group and the control group according to random number table method with 70 cases in each group. The control group was treated with citalopram, while the observation group was treated with HBO combined with citalopram. After 4 weeks treatment, the clinical effect, depression score, sleep disorder, serum interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and cortisol levels of the two groups were observed.Results:After 4 weeks of treatment, the total effective rate of the observation group (94.29%) was significantly higher than that of the control group (77.14%), with statistically significant difference ( P<0.05). After treatment, the scores of sleep quality, sleep time, and depressive state of the two groups decreased significantly than those before treatment, and the decrease in the observation group was more significant after treatment, with statistically significant differences ( P<0.05 or P<0.01). The levels of IL-1, IL-6, TNF-α and cortisol in the two groups decreased significantly after treatment, and the decrease of the observation group was more significant, with statistically significant differences ( P<0.05 or P<0.01). Conclusion:HBO combined with citalopram could reduce the levels of serum IL-1, IL-6, TNF-α, and cortisol in patients with depression suffering from sleep disorders, and significantly improve depression and sleep disorders, indicating it can achieve satisfactory clinic effect.

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