OBJECTIVE: To investigate the consistency between FCM and PCR on the detecting of MRD in TCF3-PBX1 METHODS: 55 cases of paediatric TCF3-PBX1 RESULTS: Among the 55 children with TCF3-PBX1 CONCLUSION The detection result of MRD in TCF3-PBX1 detect by FCM and PCR shows better consistency. MRD positivity detected by FCM at the end of induction therapy (day 33) predicts a high risk of relapse in TCF3-PBX1 ALL patients.
Adolescent ; Bone Marrow ; Child ; Child, Preschool ; Female ; Humans ; Male ; Neoplasm, Residual ; Oncogene Proteins, Fusion/genetics* ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; Prognosis ; Recurrence

Germ-free animals and metagenomic technology are the two major wheels for the study of microbiome in human health. Without a transplantation model of germ?free animals inoculated with microbiota (bacterial strains), it is impossible to establish the causal relationship between microbiota and diseases. Without germ?free animals, there will be no rapid development of research on the relationship between microbiota and human diseases today and tomorrow. Application of germ?free animals has formed four kinds of general research models, including comparison of conventional and germ?free animals, bacteria ( strain) transplantation with germ?free animals, rederivation of germ?free genetic engineering animals, and development of germ?free animals. Standardization of research mode will increase the scale and speed of application of germ?free animals. After ten years of efforts, Professor Wei Hong' s group has built the largest scale, the most stable tech?nical system, most widely used platform of germ?free animal in our country, which have provided a strong support in our country for scientific problem solving in the fields including animal husbandry, biomedicine, and food microbiology. At present, the germ?free animals are in small scale, low in efficiency, and the platform is separated from the application con?ditions. The basic theory and technical system of germ?free animals are still to be developed, and it is difficult to meet the growing demand for application. Establishment of an efficient large?scale research and application system of germ?free ani?mals will meet the needs of rapid development of microbiome research in China.

Neonatal necrotizing enterocolitis ( NEC) is a severe gastrointestinal disease with high mortality. Stud-ies have suggested that NEC are associated with prematurity and very?low birth weight, but the exact etiology and pathogen?esis has not been elucidated. Therefore, it is important to build an ideal NEC model to explore the pathogenesis and pre?vention measures of NEC to reduce the incidence and severity of NEC.

The role of intestinal microbiota in mammals and humans are gaining increasing attention. The health of piglets requires a dynamically balanced intestinal microbiota. However, there are temporal and spatial changes in the distri-bution and composition of microbiota from the esophagus to the rectum during the life cycle of the pig. The intestinal micro?biota has various beneficial functions in pig, like nutrient metabolism, intestinal mucosal barrier, immune responses and pathogen infection. A variety of factors play an important role in the formation and stabilization of intestinal microbiota, in?cluding the ways of delivery ( vaginal or caesarean) , the diet during infancy ( breast milk or formula feeds) and the usage of antibiotics or antibiotic-like molecules. In this review, we mainly discussed the relationship between intestinal microbi?ota and the intestinal health of piglets from the aspects of the composition and colonization of intestinal microbiota, as well as the functions and influencing factors of intestinal microbiota, so as to further understanding the importance of intestinal microbiota in intestinal function of piglets.

The effect of dietary intervention on intestinal flora and health has become a research focus in the medi-cal and health field. In terms of development, intestinal flora may become an important target for the study of the influence of dietary styl, health food and traditional Chinese medicine on human health. However, due to the complexity of intestinal flora,high standard for animal models that is applied to researches on the relationship between intestinal flora and dietary in?terference is required. It has been claimed that there is no living microorganisms and parasites inside germ?free animals, thus they are the most widely used basic animal models in the study of intestinal flora. Therefore, it is a common way to ap?ply germ?free animal for generating human flora animal model to study the relationship between diet, flora and health. In this paper we will review the researches and applications of human source flora animal models established by germ?free ani?mals and the influence of dietary intervention on gut microbiota.

The relationship between intestinal microbiome and host growth and development, immunity, metabo-lism and other aspects is very close. But the complex interaction between intestinal microbiota and host is still largely un?known. At present, germ?free animal models have become an important tool for exploring the interaction between intestinal microbiome and host. Many studies used germ?free animal models to explore the role of gut microbiome in host metabolism, the development of the immune system, including the role of gut microbiome in the pathogenesis and prognosis of autoim?mune diseases. It has been found that intestinal microbiome as one of the environmental factors may be involved in the pathogenesis of rheumatoid arthritis, but its causal relationship is unknown. This paper will review the correlation between the intestinal microbiota and pathogenesis of rheumatoid arthritis by using germ?free animal models, and provide a theoreti?cal basis for further study of the role of intestinal microbiome in the pathogenesis of rheumatoid arthritis.

OBJECTIVETo investigate the incidence of karyotypes and gene mutations for elder acute myeloid leukemia and to explore the relationship between each other.

METHODSClinical data and bone marrow samples of elder AML patients were collected. Karyotype and gene mutation (FLT3, NPM1, C-Kit, CEBPα, DNMT3A) test were performed, characteristics of karyotypes and gene mutations were analysed.

RESULTSThe incidence of better risk karyotype was 16.6%, in which the incidences of t(15;17), t(8;21) and inv (16)/t(16;16) were 3.90%, 10.73%, and 1.95% respectively; the incidence of intermediate risk karyotype was 72.2%, in which the incidence of normal karyotype was 57.86%; the incidence of poor risk karyotype was 11.20%, in which the incidence of of MLL/11q23, complex karyotype and monosomal karyotype were 1.95%, 6.34%, 5.85% respectively; the incidences of FLT3, NPM1, C-Kit, CEBPα, DNMT3A mutation were 12.57%, 22.06%, 2.16%, 14.71%, 15.71% respectively. Compared with patients older than 60 years, patients with age of 55-60 years were with less complex karyotype (1.09% vs 10.62%)(P=0.003) and monosomal karyotype (2.17% vs 8.85%)(P=0.032), and more t(8;21)(17.39% vs 5.31%)(P=0.008) and inv (16)/t(16;16)(4.35% vs 0.00%)(P=0.045).

CONCLUSIONFor older AML patients, great difference in the distribution of karyotyes was found between the patients older than 60 years and patients with age of 55-60 years, while no such characteristics was found for gene mutations. Good elucidation of karyotypes and gene mutations are key for the treatment of older acute myeloid leukemia patients.

Humans ; Incidence ; Karyotype ; Karyotyping ; Middle Aged ; Mutation ; Proto-Oncogene Proteins c-kit

OBJECTIVETo explore the effect of Wnt signaling suppression on proliferation of non small cell lung cancer to gefitinib, and its related mechanisms.

METHODSPC9 and PC9/AB2 cells of both gefitinib sensitive and resistant were treated with different concentrations of gefitinib, and the proliferation index was measured using CCK8 kit. The members of Wnt signaling pathway were detected by Western blot. Dual luciferase reportor gene assay (TOP Flash) was used to document the transcriptional level of β-catenin. β-catenin siRNA was transfected into PC9/AB2 cells to suppress the Wnt signaling transcription, followed by treatment with different concentrations of gefitinib. Western blot was then used to detect the expression of EGFR and its downstream signaling after inhibit the expression of β-catenin.

RESULTSTreating with different concentrations of gefitinib, the resistance of PC9/AB2 cells to gefitinib was significantly increased (P < 0.05). The members of Wnt signaling expressed at higher level in PC9/AB2 cells than in PC9 cells (t = 24.590, P = 0.000). TOP Flash examination showed that the endogenous transcriptional activity of Wnt signaling was higher in PC9/AB2 cell than that in PC9 cell (t = 4.983, P = 0.008). Compared with the negative control group, apoptotic rate and sensitivity to gefitinib significantly increased in interfered group (P < 0.05). The expression of p-ERK1/2 significantly decreased after Wnt signaling suppression, although other proteins showed no significant alterations.

CONCLUSIONSuppressing the activity of Wnt signaling can partly reverse the celluar resistance to gefitinib in non small cell lung cancer.

Antineoplastic Agents ; administration & dosage ; pharmacology ; Apoptosis ; Carcinoma, Non-Small-Cell Lung ; metabolism ; pathology ; Cell Line, Tumor ; Cell Proliferation ; Dose-Response Relationship, Drug ; Drug Resistance, Neoplasm ; Humans ; Lung Neoplasms ; metabolism ; pathology ; Mitogen-Activated Protein Kinase 1 ; metabolism ; Mitogen-Activated Protein Kinase 3 ; metabolism ; Phosphorylation ; Quinazolines ; administration & dosage ; pharmacology ; Wnt Signaling Pathway ; drug effects ; beta Catenin ; metabolism

Objective To observe the effect of Nur77 on lipid loading in macrophages exposed to 40 μg/ml oxidized low density iipoprotein (ox-LDL).Methods Stable RAW264.7 strain expressing green fluorescent protein (GFP) or GFP-Nur77 was established by G418 screening after transfection with corresponding plasmids and identified by Western blot.After 24 h stimulation with ox-LDL.intracellular lipid loading of each strain was observed by Oil Red O dyeing.and the intracellular cholesterol level was measured by liquid chromatographic-mass spectrometry (LC-MS).The transcriptional changes of CD36 and ABCA1 were monitored by Real Time Quantitative-PCR,while the expressions of these two proteins were assayed by flow cytometry and Western blot,respectively.Results After 24 h stimulation with ox-LDL,intracellular total cholesterol and esterified cholesterol concentration in GFP-Nur77-RAW264.7 were significantly dropped by 26.15% and 30.93% restxectively (P＜0.05 vs.GFP-RAW264.7).The transcription and expression of ABCA1 in GFP-Nur77-RAW264.7 were significantly increased while the transcription and expression of CD36 were significantly reduced (all P＜0.05 vs.GFP-RAW264.7).Conclusion Orphan nuclear receptor Nur77 reduced ox-LDL induced intracellular lipid loading in macrophages by inhibiting lipi d influx and enhancing lipid efflux.

OBJECTIVETo investigate the effect and related mechanism of retinoid X receptor (RXR) activation on oxidized low-density lipoprotein (ox-LDL) induced differentiation of macrophage into dendritic cell.

METHODSRAW264.7 murine macrophage cell line was cultured with ox-LDL for 48 h in the absence and presence of RXR activator 9-cisRA or SR11237. Cell morphology was observed by phase contrast microscope and cell surface markers involved in dendritic cell immune maturation and activation was analyzed by FACS. Cellular reactive oxygen species production was detected by CM-H2DCFDA fluorescent probe.

RESULTSox-LDL-treated RAW264.7 murine macrophage cell line differentiated into dendritic like cells after 48 h and cell surface markers CD40, CD86, CD83, MHC Class II and CD1d were upregulated. These changes could be attenuated by cotreatment with 9-cisRA or SR11237. Upregulated cell surface markers CD40, CD86, CD83, MHC Class II and CD1d by ox-LDL were decreased about 47%, 43%, 48%, 32% and 17% respectively by 9-cisRA and 38%, 38%, 46%, 36% and 32% respectively by SR11237. The effect of 9-cisRA and SR11237 was dose dependent. Cellular reactive oxygen species were significantly increased in ox-LDL-treated RAW264.7 cells (MFI 38.24 +/- 4.20 vs. 4.46 +/- 0.39, P < 0.05) and which was significantly reduced by 9-cisRA (10(-7) mol/L) and SR11237 (10(-6) mol/L) to 12.60 +/- 1.52 and 17.89 +/- 1.91 respectively (all P < 0.05).

CONCLUSIONRXR activation partly inhibits the differentiation of ox-LDL induced macrophage into dendritic cell by reducing oxidative stress injury.

Animals ; Benzoates ; pharmacology ; Cell Differentiation ; drug effects ; Cell Line ; Dendritic Cells ; cytology ; drug effects ; Lipoproteins, LDL ; metabolism ; Macrophages ; cytology ; drug effects ; Mice ; Retinoid X Receptors ; metabolism ; Retinoids ; pharmacology ; Tretinoin ; pharmacology